AcbaC.17683.a
3-dehydroquinate synthase
| CENTER ID: | AcbaC.17683.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17683.a.B1.GE38759 | full length | 1 | 360 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17683.a.B1.PS38052 | full length | 1 | 360 |
Structures
5EKS
DEPOSITED: 11/4/2015
DETERMINATION: XRay
CLONE: AcbaC.17683.a.B1.GE38759
PROTEIN: AcbaC.17683.a.B1.PS02371
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30082.1 |
| PATRIC ID: | fig|470.1345.peg.283 |
| PGAT: | acinetobacter:ABUW_0296 |
| RefSeq: | WP_001193997.1 |
| UniProt: | V5V8R5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQTLHVELGE RRYPIFIGSQ LDPKQLLEPY IHGQQVMIVS NVTVAPLYLS HYQEALESLG KTVATCILPD GEKYKDIQHL NLIFDALLEA GFNRDCTVLA LGGGVIGDMA GFASACFQRG VYFVQVPTTL LSQVDSSVGG KTGINHPLGK NMLGAFQQPQ VVLADMAQLN TLPERELSAG LAEVIKYALL GDEDFLVWLE ENMDGLVARD ADLLAEAVYR SCAHKARIVA NDEKEQGERA LLNLGHTFGH AIESYLGYGT WLHGEAVATG MVMAADLSQR LGWISNEDVA RTKKIIQRAN LPISCPQIPL DDFLGYMAHD KKVLNGQLRL VLLKQLGQAV ITKDFDVELM KQAILANQHG
NT Sequence
ATGCAAACTT TACATGTTGA ATTAGGCGAA CGCCGTTATC CTATTTTCAT TGGTAGCCAA CTTGACCCGA AACAATTACT AGAGCCTTAT ATTCATGGTC AACAGGTCAT GATTGTGAGT AATGTTACGG TTGCGCCTTT ATATTTAAGC CACTATCAGG AAGCTTTGGA AAGTCTAGGT AAAACGGTTG CGACCTGTAT TTTACCTGAT GGCGAAAAGT ATAAAGATAT TCAGCATTTA AACTTAATTT TTGATGCATT GCTTGAAGCT GGTTTTAACC GCGATTGTAC GGTTTTAGCT TTAGGTGGTG GCGTCATTGG TGATATGGCT GGTTTTGCTT CTGCTTGTTT CCAACGTGGC GTTTACTTTG TACAGGTTCC TACGACCTTA CTTTCACAAG TAGACTCAAG TGTTGGTGGA AAAACAGGAA TTAATCATCC GCTTGGCAAG AATATGTTAG GTGCTTTTCA GCAACCACAA GTCGTTTTAG CTGACATGGC ACAATTGAAT ACGCTGCCAG AACGTGAGCT TTCGGCCGGT TTAGCCGAAG TAATTAAATA TGCACTTTTA GGTGATGAAG ATTTCTTGGT TTGGCTTGAA GAAAATATGG ATGGCCTGGT TGCAAGAGAT GCTGACCTTT TAGCAGAGGC TGTTTATCGT TCATGTGCTC ACAAAGCCCG CATTGTTGCC AATGATGAGA AAGAGCAAGG TGAACGTGCT TTACTTAACT TAGGTCATAC TTTTGGTCAT GCGATTGAGT CGTATCTAGG TTATGGAACT TGGTTGCATG GTGAAGCTGT AGCAACTGGA ATGGTTATGG CGGCAGATTT ATCACAACGT TTGGGTTGGA TCTCAAATGA AGATGTGGCA CGTACAAAAA AAATCATTCA ACGTGCAAAT TTGCCGATAT CATGTCCACA AATTCCATTG GATGATTTCC TTGGATATAT GGCTCATGAC AAAAAAGTTC TTAATGGGCA ATTACGTTTG GTGTTGTTAA AACAACTCGG ACAAGCTGTA ATTACTAAGG ACTTTGATGT TGAACTTATG AAACAAGCTA TTTTGGCGAA TCAACACGGA TAA
Details for AcbaC.17683.a.B1.GE38759
| HARVESTED ON: | 1/22/2015 |
| SEQUENCED ON: | 1/15/2015 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMQ TLHVELGERR YPIFIGSQLD PKQLLEPYIH GQQVMIVSNV TVAPLYLSHY QEALESLGKT VATCILPDGE KYKDIQHLNL IFDALLEAGF NRDCTVLALG GGVIGDMAGF ASACFQRGVY FVQVPTTLLS QVDSSVGGKT GINHPLGKNM LGAFQQPQVV LADMAQLNTL PERELSAGLA EVIKYALLGD EDFLVWLEEN MDGLVARDAD LLAEAVYRSC AHKARIVAND EKEQGERALL NLGHTFGHAI ESYLGYGTWL HGEAVATGMV MAADLSQRLG WISNEDVART KKIIQRANLP ISCPQIPLDD FLGYMAHDKK VLNGQLRLVL LKQLGQAVIT KDFDVELMK
Validated NT Sequence
ttgtttcata agttcaacat caaagtcctt agtaattaca gcttgtccga gttgttttaa caacaccaaa cgtaattgcc cattaagaac ttttttgtca tgagccatat atccaaggaa atcatccaat ggaatttgtg gacatgatat cggcaaattt gcacgttgaa tgattttttt tgtacgtgcc acatcttcat ttgagatcca acccaaacgt tgtgataaat ctgccgccat aaccattcca gttgctacag cttcaccatg caaccaagtt ccataaccta gatacgactc aatcgcatga ccaaaagtat gacctaagtt aagtaaagca cgttcacctt gctctttctc atcattggca acaatgcggg ctttgtgagc acatgaacga taaacagcct ctgctaaaag gtcagcatct cttgcaacca ggccatccat attttcttca agccaaacca agaaatcttc atcacctaaa agtgcatatt taattacttc ggctaaaccg gccgaaagct cacgttctgg cagcgtattc aattgtgcca tgtcagctaa aacgacttgt ggttgctgaa aagcacctaa catattcttg ccaagcggat gattaattcc tgtttttcca ccaacacttg agtctacttg tgaaagtaag gtcgtaggaa cctgtacaaa gtaaacgcca cgttggaaac aagcagaagc aaaaccagcc atatcaccaa tgacgccacc acctaaagct aaaaccgtac aatcgcggtt aaaaccagct tcaagcaatg catcaaaaat taagtttaaa tgctgaatat ctttatactt ttcgccatca ggtaaaatac aggtcgcaac cgttttacct agactttcca aagcttcctg atagtggctt aaatataaag gcgcaaccgt aacattactc acaatcatga cctgttgacc atgaatataa ggctctagta attgtttcgg gtcaagttgg ctaccaatga aaataggata acggcgttcg cctaattcaa catgtaaagt ttgcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMQ TLHVELGERR YPIFIGSQLD PKQLLEPYIH GQQVMIVSNV TVAPLYLSHY QEALESLGKT VATCILPDGE KYKDIQHLNL IFDALLEAGF NRDCTVLALG GGVIGDMAGF ASACFQRGVY FVQVPTTLLS QVDSSVGGKT GINHPLGKNM LGAFQQPQVV LADMAQLNTL PERELSAGLA EVIKYALLGD EDFLVWLEEN MDGLVARDAD LLAEAVYRSC AHKARIVAND EKEQGERALL NLGHTFGHAI ESYLGYGTWL HGEAVATGMV MAADLSQRLG WISNEDVART KKIIQRANLP ISCPQIPLDD FLGYMAHDKK VLNGQLRLVL LKQLGQAVIT KDFDVELMKQ AILANQHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa ctttacatgt tgaattaggc gaacgccgtt atcctatttt cattggtagc caacttgacc cgaaacaatt actagagcct tatattcatg gtcaacaggt catgattgtg agtaatgtta cggttgcgcc tttatattta agccactatc aggaagcttt ggaaagtcta ggtaaaacgg ttgcgacctg tattttacct gatggcgaaa agtataaaga tattcagcat ttaaacttaa tttttgatgc attgcttgaa gctggtttta accgcgattg tacggtttta gctttaggtg gtggcgtcat tggtgatatg gctggttttg cttctgcttg tttccaacgt ggcgtttact ttgtacaggt tcctacgacc ttactttcac aagtagactc aagtgttggt ggaaaaacag gaattaatca tccgcttggc aagaatatgt taggtgcttt tcagcaacca caagtcgttt tagctgacat ggcacaattg aatacgctgc cagaacgtga gctttcggcc ggtttagccg aagtaattaa atatgcactt ttaggtgatg aagatttctt ggtttggctt gaagaaaata tggatggcct ggttgcaaga gatgctgacc ttttagcaga ggctgtttat cgttcatgtg ctcacaaagc ccgcattgtt gccaatgatg agaaagagca aggtgaacgt gctttactta acttaggtca tacttttggt catgcgattg agtcgtatct aggttatgga acttggttgc atggtgaagc tgtagcaact ggaatggtta tggcggcaga tttatcacaa cgtttgggtt ggatctcaaa tgaagatgtg gcacgtacaa aaaaaatcat tcaacgtgca aatttgccga tatcatgtcc acaaattcca ttggatgatt tccttggata tatggctcat gacaaaaaag ttcttaatgg gcaattacgt ttggtgttgt taaaacaact cggacaagct gtaattacta aggactttga tgttgaactt atgaaacaag ctattttggc gaatcaacac ggatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.17683.a.B1.PS38052
| PURIFICATION DATe: | 9/21/2016 |
| CONCENTRATION: | 40mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ TLHVELGERR YPIFIGSQLD PKQLLEPYIH GQQVMIVSNV TVAPLYLSHY QEALESLGKT VATCILPDGE KYKDIQHLNL IFDALLEAGF NRDCTVLALG GGVIGDMAGF ASACFQRGVY FVQVPTTLLS QVDSSVGGKT GINHPLGKNM LGAFQQPQVV LADMAQLNTL PERELSAGLA EVIKYALLGD EDFLVWLEEN MDGLVARDAD LLAEAVYRSC AHKARIVAND EKEQGERALL NLGHTFGHAI ESYLGYGTWL HGEAVATGMV MAADLSQRLG WISNEDVART KKIIQRANLP ISCPQIPLDD FLGYMAHDKK VLNGQLRLVL LKQLGQAVIT KDFDVELMK
Validated NT Sequence
ttgtttcata agttcaacat caaagtcctt agtaattaca gcttgtccga gttgttttaa caacaccaaa cgtaattgcc cattaagaac ttttttgtca tgagccatat atccaaggaa atcatccaat ggaatttgtg gacatgatat cggcaaattt gcacgttgaa tgattttttt tgtacgtgcc acatcttcat ttgagatcca acccaaacgt tgtgataaat ctgccgccat aaccattcca gttgctacag cttcaccatg caaccaagtt ccataaccta gatacgactc aatcgcatga ccaaaagtat gacctaagtt aagtaaagca cgttcacctt gctctttctc atcattggca acaatgcggg ctttgtgagc acatgaacga taaacagcct ctgctaaaag gtcagcatct cttgcaacca ggccatccat attttcttca agccaaacca agaaatcttc atcacctaaa agtgcatatt taattacttc ggctaaaccg gccgaaagct cacgttctgg cagcgtattc aattgtgcca tgtcagctaa aacgacttgt ggttgctgaa aagcacctaa catattcttg ccaagcggat gattaattcc tgtttttcca ccaacacttg agtctacttg tgaaagtaag gtcgtaggaa cctgtacaaa gtaaacgcca cgttggaaac aagcagaagc aaaaccagcc atatcaccaa tgacgccacc acctaaagct aaaaccgtac aatcgcggtt aaaaccagct tcaagcaatg catcaaaaat taagtttaaa tgctgaatat ctttatactt ttcgccatca ggtaaaatac aggtcgcaac cgttttacct agactttcca aagcttcctg atagtggctt aaatataaag gcgcaaccgt aacattactc acaatcatga cctgttgacc atgaatataa ggctctagta attgtttcgg gtcaagttgg ctaccaatga aaataggata acggcgttcg cctaattcaa catgtaaagt ttgcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMQ TLHVELGERR YPIFIGSQLD PKQLLEPYIH GQQVMIVSNV TVAPLYLSHY QEALESLGKT VATCILPDGE KYKDIQHLNL IFDALLEAGF NRDCTVLALG GGVIGDMAGF ASACFQRGVY FVQVPTTLLS QVDSSVGGKT GINHPLGKNM LGAFQQPQVV LADMAQLNTL PERELSAGLA EVIKYALLGD EDFLVWLEEN MDGLVARDAD LLAEAVYRSC AHKARIVAND EKEQGERALL NLGHTFGHAI ESYLGYGTWL HGEAVATGMV MAADLSQRLG WISNEDVART KKIIQRANLP ISCPQIPLDD FLGYMAHDKK VLNGQLRLVL LKQLGQAVIT KDFDVELMKQ AILANQHG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA CTTTACATGT TGAATTAGGC GAACGCCGTT ATCCTATTTT CATTGGTAGC CAACTTGACC CGAAACAATT ACTAGAGCCT TATATTCATG GTCAACAGGT CATGATTGTG AGTAATGTTA CGGTTGCGCC TTTATATTTA AGCCACTATC AGGAAGCTTT GGAAAGTCTA GGTAAAACGG TTGCGACCTG TATTTTACCT GATGGCGAAA AGTATAAAGA TATTCAGCAT TTAAACTTAA TTTTTGATGC ATTGCTTGAA GCTGGTTTTA ACCGCGATTG TACGGTTTTA GCTTTAGGTG GTGGCGTCAT TGGTGATATG GCTGGTTTTG CTTCTGCTTG TTTCCAACGT GGCGTTTACT TTGTACAGGT TCCTACGACC TTACTTTCAC AAGTAGACTC AAGTGTTGGT GGAAAAACAG GAATTAATCA TCCGCTTGGC AAGAATATGT TAGGTGCTTT TCAGCAACCA CAAGTCGTTT TAGCTGACAT GGCACAATTG AATACGCTGC CAGAACGTGA GCTTTCGGCC GGTTTAGCCG AAGTAATTAA ATATGCACTT TTAGGTGATG AAGATTTCTT GGTTTGGCTT GAAGAAAATA TGGATGGCCT GGTTGCAAGA GATGCTGACC TTTTAGCAGA GGCTGTTTAT CGTTCATGTG CTCACAAAGC CCGCATTGTT GCCAATGATG AGAAAGAGCA AGGTGAACGT GCTTTACTTA ACTTAGGTCA TACTTTTGGT CATGCGATTG AGTCGTATCT AGGTTATGGA ACTTGGTTGC ATGGTGAAGC TGTAGCAACT GGAATGGTTA TGGCGGCAGA TTTATCACAA CGTTTGGGTT GGATCTCAAA TGAAGATGTG GCACGTACAA AAAAAATCAT TCAACGTGCA AATTTGCCGA TATCATGTCC ACAAATTCCA TTGGATGATT TCCTTGGATA TATGGCTCAT GACAAAAAAG TTCTTAATGG GCAATTACGT TTGGTGTTGT TAAAACAACT CGGACAAGCT GTAATTACTA AGGACTTTGA TGTTGAACTT ATGAAACAAG CTATTTTGGC GAATCAACAC GGATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT