AcbaC.17399.a

oxidoreductase

CENTER ID: AcbaC.17399.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17399.a.B1.GE38755 full length 1 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17399.a.B1.PS02414 full length 1 356

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1069
GenBank: AKA30856.1
PGAT: acinetobacter:ABUW_1104
RefSeq: WP_001147890.1
UniProt: A0A0D5YGH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQAIEKRNSL FNSLTQAVFD KNMANFWLQK INPLWSVQHG LVQIVKKEFV AHDTVSLTLK CNRLVKMGAA GQHHPVIVEI AGRRYERTYS LTQIDAEHLR LTVKKVADGI VSNWFIAESK IGDVFELGQP YGDMQENIQT PKLIMLAAGS GITPMLSLIT AIKQSQQLEK TQVQLLYWVK QRSDAAFTEY FEQVAEQNPN FSYQVFYTQE TPNDERLNAE HLALVDGIEN STVYACGPSG FVSTVEQLFE KAPTVLTEAF SLTNESSADD IGYVNVTLTQ SNKVIAIPKG QSILVSLEHE GLKPTHGCRM GICNKCVCSK TQGSTRNLLN GSQNTEPSQL LKICVNSAQS DLVIDL
NT Sequence
ATGCAAGCAA TCGAAAAGAG AAATTCTCTA TTCAATTCAT TAACACAAGC TGTATTTGAC AAAAATATGG CGAACTTCTG GCTACAAAAA ATAAATCCTT TATGGTCTGT GCAGCATGGA CTAGTTCAAA TTGTAAAAAA AGAATTCGTA GCTCATGACA CTGTGAGCCT GACATTAAAA TGCAACCGCT TGGTGAAAAT GGGTGCGGCA GGTCAACATC ATCCAGTGAT TGTTGAAATT GCGGGCCGTC GTTATGAACG TACTTATAGC TTGACGCAAA TTGATGCAGA GCACTTACGT TTAACAGTTA AAAAAGTTGC TGATGGTATT GTCAGTAACT GGTTTATTGC AGAAAGCAAA ATTGGTGATG TTTTTGAGCT TGGGCAGCCT TATGGTGACA TGCAAGAAAA CATTCAGACA CCGAAATTAA TTATGTTAGC TGCTGGTAGT GGTATTACAC CAATGTTGAG CTTAATCACA GCAATTAAGC AAAGCCAACA GTTAGAAAAA ACGCAGGTTC AGCTTTTATA TTGGGTCAAA CAGCGTTCTG ATGCTGCTTT TACAGAGTAC TTTGAACAAG TGGCTGAACA AAACCCAAAC TTTAGCTATC AGGTGTTTTA CACACAAGAA ACACCAAATG ATGAACGTTT AAATGCCGAA CATCTTGCAC TGGTTGATGG TATTGAAAAC AGCACAGTTT ATGCATGTGG TCCATCGGGC TTTGTTTCAA CTGTTGAACA GCTTTTTGAA AAAGCACCAA CAGTACTTAC TGAAGCATTT AGTTTAACTA ATGAAAGTTC TGCGGATGAC ATTGGTTATG TGAATGTCAC ATTAACCCAG TCTAATAAAG TGATTGCAAT TCCTAAAGGC CAATCCATTT TGGTCAGCCT TGAGCATGAA GGCCTTAAGC CGACACATGG CTGTCGTATG GGAATTTGTA ATAAATGTGT TTGTAGTAAA ACGCAAGGTT CTACACGTAA TTTACTAAAC GGTAGCCAAA ATACGGAACC AAGCCAATTA CTTAAAATTT GTGTGAACTC AGCACAATCC GATCTAGTTA TTGATCTTTA A
Details for AcbaC.17399.a.B1.GE38755
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ AIEKRNSLFN SLTQAVFDKN MANFWLQKIN PLWSVQHGLV QIVKKEFVAH DTVSLTLKCN RLVKMGAAGQ HHPVIVEIAG RRYERTYSLT QIDAEHLRLT VKKVADGIVS NWFIAESKIG DVFELGQPYG DMQENIQTPK LIMLAAGSGI TPMLSLITAI KQSQQLEKTQ VQLLYWVKQR SDAAFTEYFE QVAEQNPNFS YQVFYTQETP NDERLNAEHL ALVDGIENST VYACGPSGFV STVEQLFEKA PTVLTEAFSL TNESSADDIG YVNVTLTQSN KVIAIPKGQS ILVSLEHEGL KPTHGCRMGI CNKCVCSKTQ GSTRNLLNGS QNTEPSQLLK ICVNSAQ
Validated NT Sequence
attgtgctga gttcacacaa attttaagta attggcttgg ttccgtattt tggctaccgt ttagtaaatt acgtgtagaa ccttgcgttt tactacaaac acatttatta caaattccca tacgacagcc atgtgtcggc ttaaggcctt catgctcaag gctgaccaaa atggattggc ctttaggaat tgcaatcact ttattagact gggttaatgt gacattcaca taaccaatgt catccgcaga actttcatta gttaaactaa atgcttcagt aagtactgtt ggtgcttttt caaaaagctg ttcaacagtt gaaacaaagc ccgatggacc acatgcataa actgtgctgt tttcaatacc atcaaccagt gcaagatgtt cggcatttaa acgttcatca tttggtgttt cttgtgtgta aaacacctga tagctaaagt ttgggttttg ttcagccact tgttcaaagt actctgtaaa agcagcatca gaacgctgtt tgacccaata taaaagctga acctgcgttt tttctaactg ttggctttgc ttaattgctg tgattaagct caacattggt gtaataccac taccagcagc taacataatt aatttcggtg tctgaatgtt ttcttgcatg tcaccataag gctgcccaag ctcaaaaaca tcaccaattt tgctttctgc aataaaccag ttactgacaa taccatcagc aactttttta actgttaaac gtaagtgctc tgcatcaatt tgcgtcaagc tataagtacg ttcataacga cggcccgcaa tttcaacaat cactggatga tgttgacctg ccgcacccat tttcaccaag cggttgcatt ttaatgtcag gctcacagtg tcatgagcta cgaattcttt ttttacaatt tgaactagtc catgctgcac agaccataaa ggatttattt tttgtagcca gaagttcgcc atatttttgt caaatacagc ttgtgttaat gaattgaata gagaatttct cttttcgatt gcttgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMQ AIEKRNSLFN SLTQAVFDKN MANFWLQKIN PLWSVQHGLV QIVKKEFVAH DTVSLTLKCN RLVKMGAAGQ HHPVIVEIAG RRYERTYSLT QIDAEHLRLT VKKVADGIVS NWFIAESKIG DVFELGQPYG DMQENIQTPK LIMLAAGSGI TPMLSLITAI KQSQQLEKTQ VQLLYWVKQR SDAAFTEYFE QVAEQNPNFS YQVFYTQETP NDERLNAEHL ALVDGIENST VYACGPSGFV STVEQLFEKA PTVLTEAFSL TNESSADDIG YVNVTLTQSN KVIAIPKGQS ILVSLEHEGL KPTHGCRMGI CNKCVCSKTQ GSTRNLLNGS QNTEPSQLLK ICVNSAQSDL VIDL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag caatcgaaaa gagaaattct ctattcaatt cattaacaca agctgtattt gacaaaaata tggcgaactt ctggctacaa aaaataaatc ctttatggtc tgtgcagcat ggactagttc aaattgtaaa aaaagaattc gtagctcatg acactgtgag cctgacatta aaatgcaacc gcttggtgaa aatgggtgcg gcaggtcaac atcatccagt gattgttgaa attgcgggcc gtcgttatga acgtacttat agcttgacgc aaattgatgc agagcactta cgtttaacag ttaaaaaagt tgctgatggt attgtcagta actggtttat tgcagaaagc aaaattggtg atgtttttga gcttgggcag ccttatggtg acatgcaaga aaacattcag acaccgaaat taattatgtt agctgctggt agtggtatta caccaatgtt gagcttaatc acagcaatta agcaaagcca acagttagaa aaaacgcagg ttcagctttt atattgggtc aaacagcgtt ctgatgctgc ttttacagag tactttgaac aagtggctga acaaaaccca aactttagct atcaggtgtt ttacacacaa gaaacaccaa atgatgaacg tttaaatgcc gaacatcttg cactggttga tggtattgaa aacagcacag tttatgcatg tggtccatcg ggctttgttt caactgttga acagcttttt gaaaaagcac caacagtact tactgaagca tttagtttaa ctaatgaaag ttctgcggat gacattggtt atgtgaatgt cacattaacc cagtctaata aagtgattgc aattcctaaa ggccaatcca ttttggtcag ccttgagcat gaaggcctta agccgacaca tggctgtcgt atgggaattt gtaataaatg tgtttgtagt aaaacgcaag gttctacacg taatttacta aacggtagcc aaaatacgga accaagccaa ttacttaaaa tttgtgtgaa ctcagcacaa tccgatctag ttattgatct ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for AcbaC.17399.a.B1.PS02414
PURIFICATION DATe: 6/3/2015
CONCENTRATION: 33.72mg/ml
OBSERVED MW: 41kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ AIEKRNSLFN SLTQAVFDKN MANFWLQKIN PLWSVQHGLV QIVKKEFVAH DTVSLTLKCN RLVKMGAAGQ HHPVIVEIAG RRYERTYSLT QIDAEHLRLT VKKVADGIVS NWFIAESKIG DVFELGQPYG DMQENIQTPK LIMLAAGSGI TPMLSLITAI KQSQQLEKTQ VQLLYWVKQR SDAAFTEYFE QVAEQNPNFS YQVFYTQETP NDERLNAEHL ALVDGIENST VYACGPSGFV STVEQLFEKA PTVLTEAFSL TNESSADDIG YVNVTLTQSN KVIAIPKGQS ILVSLEHEGL KPTHGCRMGI CNKCVCSKTQ GSTRNLLNGS QNTEPSQLLK ICVNSAQ
Validated NT Sequence
attgtgctga gttcacacaa attttaagta attggcttgg ttccgtattt tggctaccgt ttagtaaatt acgtgtagaa ccttgcgttt tactacaaac acatttatta caaattccca tacgacagcc atgtgtcggc ttaaggcctt catgctcaag gctgaccaaa atggattggc ctttaggaat tgcaatcact ttattagact gggttaatgt gacattcaca taaccaatgt catccgcaga actttcatta gttaaactaa atgcttcagt aagtactgtt ggtgcttttt caaaaagctg ttcaacagtt gaaacaaagc ccgatggacc acatgcataa actgtgctgt tttcaatacc atcaaccagt gcaagatgtt cggcatttaa acgttcatca tttggtgttt cttgtgtgta aaacacctga tagctaaagt ttgggttttg ttcagccact tgttcaaagt actctgtaaa agcagcatca gaacgctgtt tgacccaata taaaagctga acctgcgttt tttctaactg ttggctttgc ttaattgctg tgattaagct caacattggt gtaataccac taccagcagc taacataatt aatttcggtg tctgaatgtt ttcttgcatg tcaccataag gctgcccaag ctcaaaaaca tcaccaattt tgctttctgc aataaaccag ttactgacaa taccatcagc aactttttta actgttaaac gtaagtgctc tgcatcaatt tgcgtcaagc tataagtacg ttcataacga cggcccgcaa tttcaacaat cactggatga tgttgacctg ccgcacccat tttcaccaag cggttgcatt ttaatgtcag gctcacagtg tcatgagcta cgaattcttt ttttacaatt tgaactagtc catgctgcac agaccataaa ggatttattt tttgtagcca gaagttcgcc atatttttgt caaatacagc ttgtgttaat gaattgaata gagaatttct cttttcgatt gcttgcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMQ AIEKRNSLFN SLTQAVFDKN MANFWLQKIN PLWSVQHGLV QIVKKEFVAH DTVSLTLKCN RLVKMGAAGQ HHPVIVEIAG RRYERTYSLT QIDAEHLRLT VKKVADGIVS NWFIAESKIG DVFELGQPYG DMQENIQTPK LIMLAAGSGI TPMLSLITAI KQSQQLEKTQ VQLLYWVKQR SDAAFTEYFE QVAEQNPNFS YQVFYTQETP NDERLNAEHL ALVDGIENST VYACGPSGFV STVEQLFEKA PTVLTEAFSL TNESSADDIG YVNVTLTQSN KVIAIPKGQS ILVSLEHEGL KPTHGCRMGI CNKCVCSKTQ GSTRNLLNGS QNTEPSQLLK ICVNSAQSDL VIDL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAG CAATCGAAAA GAGAAATTCT CTATTCAATT CATTAACACA AGCTGTATTT GACAAAAATA TGGCGAACTT CTGGCTACAA AAAATAAATC CTTTATGGTC TGTGCAGCAT GGACTAGTTC AAATTGTAAA AAAAGAATTC GTAGCTCATG ACACTGTGAG CCTGACATTA AAATGCAACC GCTTGGTGAA AATGGGTGCG GCAGGTCAAC ATCATCCAGT GATTGTTGAA ATTGCGGGCC GTCGTTATGA ACGTACTTAT AGCTTGACGC AAATTGATGC AGAGCACTTA CGTTTAACAG TTAAAAAAGT TGCTGATGGT ATTGTCAGTA ACTGGTTTAT TGCAGAAAGC AAAATTGGTG ATGTTTTTGA GCTTGGGCAG CCTTATGGTG ACATGCAAGA AAACATTCAG ACACCGAAAT TAATTATGTT AGCTGCTGGT AGTGGTATTA CACCAATGTT GAGCTTAATC ACAGCAATTA AGCAAAGCCA ACAGTTAGAA AAAACGCAGG TTCAGCTTTT ATATTGGGTC AAACAGCGTT CTGATGCTGC TTTTACAGAG TACTTTGAAC AAGTGGCTGA ACAAAACCCA AACTTTAGCT ATCAGGTGTT TTACACACAA GAAACACCAA ATGATGAACG TTTAAATGCC GAACATCTTG CACTGGTTGA TGGTATTGAA AACAGCACAG TTTATGCATG TGGTCCATCG GGCTTTGTTT CAACTGTTGA ACAGCTTTTT GAAAAAGCAC CAACAGTACT TACTGAAGCA TTTAGTTTAA CTAATGAAAG TTCTGCGGAT GACATTGGTT ATGTGAATGT CACATTAACC CAGTCTAATA AAGTGATTGC AATTCCTAAA GGCCAATCCA TTTTGGTCAG CCTTGAGCAT GAAGGCCTTA AGCCGACACA TGGCTGTCGT ATGGGAATTT GTAATAAATG TGTTTGTAGT AAAACGCAAG GTTCTACACG TAATTTACTA AACGGTAGCC AAAATACGGA ACCAAGCCAA TTACTTAAAA TTTGTGTGAA CTCAGCACAA TCCGATCTAG TTATTGATCT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT