AcbaC.17391.a

RNA polymerase sigma factor sigma-70

CENTER ID: AcbaC.17391.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17391.a.B1.GE43939 full length 1 628
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.828
GenBank: AKA30622.1
PGAT: acinetobacter:ABUW_0862
RefSeq: WP_001281941.1
UniProt: V5V9Y3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDMHSPTSQ VAALISRGKE QGYLTYAEVN DHLPDSITES EQIEDIIQML QDVGIPVHER APESDDTMFG ESADATDEVA EEEAAAVLAS VENEPGRTTD PVRMYMREMG TVELLTREGE ISIAKRIEEG IRDVLNSIAY WPNAVEVVLK EYNDFLTGER RLADILSGYL DPETDEDIPE VLEDVEELEE EDESSTKSTK EVKLDDDDEE EESEGDDDSE GDSGPDPEVA KVRFAELEAA WAQTKAVIEK HGRNSPEANE ALESLATVFM MFKFTPRLFD IISEMIRGTH EQIRANEREI MRYAVRRGRM DRNQFRTSFP KQESNPAWLD EQIAKAPAEI KAHLEKVRPD VLAFQQKIAD IEKELGLNVS EIKDISKRMA IGEAKARRAK KEMVEANLRL VISIAKKYTN RGLQFLDLIQ EGNIGLMKAV DKFEYRRGYK FSTYATWWIR QAITRSIADQ ARTIRIPVHM IETINKINRV SRQLLQEMGR EPTPEELGER LEMDEVKVRK VLKIAKEPIS METPIGDDED SHLGDFIEDQ NITSPIDAAT SEGLKEATRE VLENLTEREA KVLKMRFGID MPTDHTLEEV GKQFDVTRER IRQIEAKALR KLRHPSRSEH LRSFLEND
NT Sequence
ATGAGCGATA TGCATTCCCC TACTTCTCAA GTAGCGGCTC TGATTAGCCG AGGCAAAGAA CAAGGTTACT TAACTTACGC TGAGGTTAAC GATCATCTGC CGGACTCGAT TACTGAAAGC GAACAGATTG AAGACATCAT CCAGATGCTT CAAGACGTTG GTATTCCTGT ACACGAGCGT GCACCTGAAT CTGACGATAC CATGTTCGGC GAATCAGCTG ATGCAACTGA TGAAGTTGCT GAAGAAGAAG CTGCTGCCGT ACTTGCATCT GTTGAAAATG AGCCTGGACG TACGACAGAC CCTGTACGTA TGTACATGCG TGAAATGGGT ACAGTAGAAC TGTTAACCCG TGAAGGTGAA ATCAGCATTG CTAAACGTAT TGAAGAAGGC ATTCGCGATG TCTTGAACTC AATCGCTTAC TGGCCAAATG CTGTTGAAGT TGTATTAAAA GAATATAATG ATTTTTTAAC TGGCGAACGC CGTCTTGCCG ATATCTTATC TGGTTATTTA GACCCTGAAA CTGATGAAGA TATTCCTGAA GTTTTAGAAG ACGTTGAAGA GCTTGAGGAA GAAGACGAAT CATCTACAAA GTCAACGAAA GAAGTTAAGC TTGATGATGA CGATGAAGAA GAAGAGTCTG AAGGTGATGA TGATTCTGAA GGTGATTCAG GTCCAGACCC TGAAGTTGCA AAAGTTCGTT TTGCTGAATT AGAAGCTGCA TGGGCTCAAA CTAAAGCTGT CATCGAAAAA CATGGCCGTA ATAGCCCTGA AGCAAATGAA GCTCTTGAGT CTTTAGCAAC TGTATTTATG ATGTTTAAAT TTACTCCACG TTTATTTGAC ATCATTTCAG AAATGATTCG CGGAACTCAT GAACAAATTC GTGCCAATGA ACGTGAAATT ATGCGTTATG CAGTACGTCG TGGCCGTATG GACCGTAACC AGTTCCGTAC ATCTTTCCCG AAACAGGAAT CTAATCCTGC TTGGTTAGAT GAACAAATTG CTAAAGCTCC AGCTGAAATC AAGGCTCACT TGGAAAAAGT TCGTCCTGAT GTTTTAGCCT TCCAGCAAAA AATTGCAGAT ATCGAAAAAG AGCTTGGCCT AAACGTTAGT GAAATTAAAG ATATTTCTAA ACGTATGGCA ATTGGTGAAG CCAAAGCTCG TCGTGCGAAA AAAGAAATGG TAGAGGCCAA CCTTCGTTTG GTAATTTCGA TTGCGAAAAA ATATACCAAC CGTGGTTTAC AGTTCCTTGA CTTGATTCAA GAAGGGAACA TTGGTTTGAT GAAAGCCGTA GACAAGTTTG AATACCGTCG TGGTTATAAA TTCTCGACTT ATGCGACTTG GTGGATTCGT CAGGCAATTA CCCGTTCTAT TGCCGATCAG GCTCGTACAA TTCGTATTCC TGTACACATG ATTGAAACGA TTAACAAAAT TAACCGTGTA TCGCGTCAAT TATTACAGGA AATGGGCCGT GAACCTACTC CTGAAGAGTT AGGCGAACGC TTAGAAATGG ATGAGGTTAA AGTTCGTAAG GTTCTTAAAA TTGCGAAAGA GCCAATTTCA ATGGAAACTC CAATCGGTGA TGATGAAGAT TCGCATCTTG GGGATTTTAT TGAAGACCAG AACATCACTT CTCCAATTGA TGCTGCGACT TCAGAAGGCT TAAAAGAAGC AACTCGTGAA GTTTTAGAAA ACTTAACTGA ACGTGAAGCA AAAGTATTGA AGATGCGTTT TGGTATTGAT ATGCCAACCG ATCATACTTT AGAAGAAGTA GGTAAGCAGT TTGATGTAAC ACGTGAGCGT ATTCGTCAGA TTGAAGCGAA AGCATTGCGT AAATTACGTC ACCCTTCTCG TTCTGAACAC TTGCGTTCTT TCCTAGAGAA TGACTAA
Details for AcbaC.17391.a.B1.GE43939
HARVESTED ON: data unavailable
SEQUENCED ON: 12/16/2020
EXPECTED MW: 73kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 47
Validated AA Sequence
MAHHHHHHMS DMHSPTSQVA ALISRGKEQG YLTYAEVNDH LPDSITESEQ IEDIIQMLQD VGIPVHERAP ESDDTMFGES ADATDEVAEE EAAAVLASVE NEPGRTTDPV RMYMREMGTV ELLTREGEIS IAKRIEEGIR DVLNSIAYWP NAVEVVLKEY NDFLTGERRL ADILSGYLDP ETDEDIPEVL EDVEELEEED ESSTKSTKEV KLDDDDEEEE SEGDDDSEGD SGPDPEVAKV RFAELEAAWA QTKAVIEKHG RNSPEANEAL ESLATVFMMF KFTPRLFDXI SEMIRXTHXX NSCQ
Validated NT Sequence
atcttactca cttagtcatt ctctaggaaa gaacgcaagt gttcagaacg agaagggtga cgtaatttac gcaatgcttt cgcttcaatc tgacgaatac gctcacgtgt tacatcaaac tgcttaccta cttcttctaa agtatgatcg gttggcatat caataccaaa acgcatcttc aatacttttg cttcacgttc agttaagttt tctaaaactt cacgagttgc ttcttttaag ccttctgaag tcgcagcatc aattggagaa gtgatgttct ggtcttcaat aaaatcccca agatgcgaat cttcatcatc accgattgga gtttccattg aaattggctc tttcgcaatt ttaagaacct tacgaacttt aacctcatcc atttctaagc gttcgcctaa ctcttcagga gtaggttcac ggcccatttc ctgtaataat tgacgcgata cacggttaat tttgttaatc gtttcaatca tgtgtacagg aatacgaatt gtacgagcct gatcggcaat agaacgggta attgcctgac gaatccacca agtcgcataa gtcgagaatt tataaccacg acggtattca aacttgtcta cggctttcat caaaccaatg ttcccttctt gaatcaagtc aaggaactgt aaaccacggt tggtatattt tttcgcaatc gaaattacca aacgaaggtt ggcctctacc atttcttttt tcgcacgacg agctttggct tcaccaattg ccatacgttt agaaatatct ttaatttcac taacgtttag gccaagctct ttttcgatat ctgcaatttt ttgctggaag gctaaaacat cnnnacgaac tttttccnag tgagccttga tttcagctgg agctttagca atttgttcat ctaaccaagc nggattagat tncntgtttc gggaaagang tannnaacnn nnnnnnnnnn ntannnnnnc nannnactgc nnaacncann atttcacgtt cattggcacg aattngntnc atgagttcnn cgaatcattt ctgaaatgan gtcaaataaa cgnggagtaa atttaaacat cataaataca gttgctaaag actcaagagc ttcatttgct tcagggctat tacggccatg tttttcgatg acagctttag tttgagccca tgcagcttct aattcagcaa aacgaacttt tgcaacttca gggtctggac ctgaatcacc ttcagaatca tcatcacctt cagactcttc ttcttcatcg tcatcatcaa gcttaacttc tttcgttgac tttgtagatg attcgtcttc ttcctcaagc tcttcaacgt cttctaaaac ttcaggaata tcttcatcag tttcagggtc taaataacca gataagatat cggcaagacg gcgttcgcca gttaaaaaat cattatattc ttttaataca acttcaacag catttggcca gtaagcgatt gagttcaaga catcgcgaat gccttcttca atacgtttag caatgctgat ttcaccttca cgggttaaca gttctactgt acccatttca cgcatgtaca tacgtacagg gtctgtcgta cgtccaggct cattttcaac agatgcaagt acggcagcag cttcttcttc agcaacttca tcagttgcat cagctgattc gccgaacatg gtatcgtcag attcaggtgc acgctcgtgt acaggaatac caacgtcttg aagcatctgg atgatgtctt caatctgttc gctttcagta atcgagtccg gcagatgatc gttaacctca gcgtaagtta agtaaccttg ttctttgcct cggctaatca gagccgctac ttgagaagta ggggaatgca tatcgctcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS DMHSPTSQVA ALISRGKEQG YLTYAEVNDH LPDSITESEQ IEDIIQMLQD VGIPVHERAP ESDDTMFGES ADATDEVAEE EAAAVLASVE NEPGRTTDPV RMYMREMGTV ELLTREGEIS IAKRIEEGIR DVLNSIAYWP NAVEVVLKEY NDFLTGERRL ADILSGYLDP ETDEDIPEVL EDVEELEEED ESSTKSTKEV KLDDDDEEEE SEGDDDSEGD SGPDPEVAKV RFAELEAAWA QTKAVIEKHG RNSPEANEAL ESLATVFMMF KFTPRLFDII SEMIRGTHEQ IRANEREIMR YAVRRGRMDR NQFRTSFPKQ ESNPAWLDEQ IAKAPAEIKA HLEKVRPDVL AFQQKIADIE KELGLNVSEI KDISKRMAIG EAKARRAKKE MVEANLRLVI SIAKKYTNRG LQFLDLIQEG NIGLMKAVDK FEYRRGYKFS TYATWWIRQA ITRSIADQAR TIRIPVHMIE TINKINRVSR QLLQEMGREP TPEELGERLE MDEVKVRKVL KIAKEPISME TPIGDDEDSH LGDFIEDQNI TSPIDAATSE GLKEATREVL ENLTEREAKV LKMRFGIDMP TDHTLEEVGK QFDVTRERIR QIEAKALRKL RHPSRSEHLR SFLEND
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg atatgcattc ccctacttct caagtagcgg ctctgattag ccgaggcaaa gaacaaggtt acttaactta cgctgaggtt aacgatcatc tgccggactc gattactgaa agcgaacaga ttgaagacat catccagatg cttcaagacg ttggtattcc tgtacacgag cgtgcacctg aatctgacga taccatgttc ggcgaatcag ctgatgcaac tgatgaagtt gctgaagaag aagctgctgc cgtacttgca tctgttgaaa atgagcctgg acgtacgaca gaccctgtac gtatgtacat gcgtgaaatg ggtacagtag aactgttaac ccgtgaaggt gaaatcagca ttgctaaacg tattgaagaa ggcattcgcg atgtcttgaa ctcaatcgct tactggccaa atgctgttga agttgtatta aaagaatata atgatttttt aactggcgaa cgccgtcttg ccgatatctt atctggttat ttagaccctg aaactgatga agatattcct gaagttttag aagacgttga agagcttgag gaagaagacg aatcatctac aaagtcaacg aaagaagtta agcttgatga tgacgatgaa gaagaagagt ctgaaggtga tgatgattct gaaggtgatt caggtccaga ccctgaagtt gcaaaagttc gttttgctga attagaagct gcatgggctc aaactaaagc tgtcatcgaa aaacatggcc gtaatagccc tgaagcaaat gaagctcttg agtctttagc aactgtattt atgatgttta aatttactcc acgtttattt gacatcattt cagaaatgat tcgcggaact catgaacaaa ttcgtgccaa tgaacgtgaa attatgcgtt atgcagtacg tcgtggccgt atggaccgta accagttccg tacatctttc ccgaaacagg aatctaatcc tgcttggtta gatgaacaaa ttgctaaagc tccagctgaa atcaaggctc acttggaaaa agttcgtcct gatgttttag ccttccagca aaaaattgca gatatcgaaa aagagcttgg cctaaacgtt agtgaaatta aagatatttc taaacgtatg gcaattggtg aagccaaagc tcgtcgtgcg aaaaaagaaa tggtagaggc caaccttcgt ttggtaattt cgattgcgaa aaaatatacc aaccgtggtt tacagttcct tgacttgatt caagaaggga acattggttt gatgaaagcc gtagacaagt ttgaataccg tcgtggttat aaattctcga cttatgcgac ttggtggatt cgtcaggcaa ttacccgttc tattgccgat caggctcgta caattcgtat tcctgtacac atgattgaaa cgattaacaa aattaaccgt gtatcgcgtc aattattaca ggaaatgggc cgtgaaccta ctcctgaaga gttaggcgaa cgcttagaaa tggatgaggt taaagttcgt aaggttctta aaattgcgaa agagccaatt tcaatggaaa ctccaatcgg tgatgatgaa gattcgcatc ttggggattt tattgaagac cagaacatca cttctccaat tgatgctgcg acttcagaag gcttaaaaga agcaactcgt gaagttttag aaaacttaac tgaacgtgaa gcaaaagtat tgaagatgcg ttttggtatt gatatgccaa ccgatcatac tttagaagaa gtaggtaagc agtttgatgt aacacgtgag cgtattcgtc agattgaagc gaaagcattg cgtaaattac gtcacccttc tcgttctgaa cacttgcgtt ctttcctaga gaatgactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at