AcbaC.17369.c

ATP synthase F1, beta subunit

CENTER ID: AcbaC.17369.c
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17369.c.B1.GE38752 full length 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3653
GenBank: AKA33407.1
PGAT: acinetobacter:ABUW_3732
RefSeq: WP_000094481.1
UniProt: V5VHQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSGRIIQII GAVIDVEFER TSVPKIYDAL QVDGTETTLE VQQQLGDGVV RTIAMGSTEG LKRGLTVTST NAPISVPVGT ATLGRIMDVL GRPIDEAGPV ATEERLPIHR QAPSYAEQAA STDLLETGIK VIDLLCPFAK GGKVGLFGGA GVGKTVNMME LINNIAKAHS GLSVFAGVGE RTREGNDFYH EMKDSNVLDK VAMVYGQMNE PPGNRLRVAL TGLTMAEYFR DEKDENGKGR DVLLFVDNIY RYTLAGTEVS ALLGRMPSAV GYQPTLAEEM GVLQERITST KSGSITSIQA VYVPADDLTD PSPATTFAHL DATVVLSRDI ASSGIYPAID PLDSTSRQLD PLVVGQEHYE IARAVQNVLQ RYKELKDIIA ILGMDELAEE DKLVVYRARK IQRFFSQPFH VAEVFTGAPG KLVPLKETIR GFKGLLAGEY DHIPEQAFYM VGGIDEVIAK AEKL
NT Sequence
ATGAGTAGCG GTCGTATCAT TCAGATCATC GGCGCGGTTA TCGACGTCGA GTTTGAGCGC ACAAGCGTTC CAAAGATCTA TGACGCTCTC CAAGTTGACG GTACTGAAAC TACTTTGGAA GTTCAGCAAC AACTTGGTGA TGGTGTTGTT CGTACCATCG CAATGGGTTC TACAGAAGGT CTTAAACGTG GTCTTACTGT AACTAGCACA AACGCACCGA TCTCTGTTCC AGTTGGTACA GCCACTCTTG GCCGTATCAT GGACGTTTTA GGTCGTCCTA TCGATGAAGC AGGTCCTGTT GCGACTGAAG AACGTTTGCC GATTCACCGT CAAGCGCCTT CTTATGCTGA ACAAGCAGCT TCTACTGACC TTTTAGAAAC TGGTATTAAA GTAATTGACT TACTTTGCCC GTTTGCGAAA GGTGGTAAAG TTGGTTTATT CGGTGGTGCT GGTGTTGGTA AAACCGTTAA CATGATGGAA TTGATCAACA ACATCGCGAA AGCACACTCA GGTTTATCTG TGTTTGCTGG TGTTGGTGAG CGTACTCGTG AAGGTAACGA CTTCTATCAC GAAATGAAAG ATTCTAACGT TCTTGACAAA GTAGCAATGG TCTACGGTCA GATGAACGAG CCACCAGGTA ACCGTTTACG CGTAGCGTTA ACTGGTTTGA CTATGGCTGA ATACTTCCGT GATGAAAAAG ACGAAAACGG TAAAGGTCGT GACGTATTAT TATTCGTCGA CAACATCTAC CGTTATACAC TTGCAGGTAC TGAAGTATCA GCATTGTTAG GTCGTATGCC ATCTGCGGTA GGTTACCAAC CTACACTTGC AGAAGAAATG GGTGTTCTTC AAGAGCGTAT TACATCTACT AAGTCTGGTT CGATCACTTC GATCCAAGCA GTATATGTAC CTGCCGATGA CTTAACAGAC CCATCGCCTG CAACTACATT TGCTCACTTA GATGCAACAG TAGTATTGAG CCGTGACATC GCATCTTCTG GTATCTATCC AGCGATCGAT CCACTTGACT CAACTTCACG TCAGTTAGAC CCATTAGTTG TTGGTCAAGA GCACTATGAA ATTGCGCGCG CAGTACAAAA CGTATTGCAA CGCTATAAAG AGCTTAAAGA CATCATCGCA ATCTTGGGTA TGGATGAATT GGCAGAAGAA GATAAATTGG TTGTTTACCG TGCGCGTAAA ATCCAACGTT TCTTCTCTCA ACCATTCCAC GTAGCTGAAG TATTTACTGG TGCTCCTGGT AAATTAGTAC CACTTAAAGA AACGATTCGT GGCTTTAAAG GTCTATTAGC TGGTGAATAC GATCACATCC CAGAACAAGC GTTCTATATG GTTGGTGGTA TTGACGAAGT GATTGCTAAA GCTGAGAAAC TCTAA
Details for AcbaC.17369.c.B1.GE38752
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 51kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS SGRIIQIIGA VIDVEFERTS VPKIYDALQV DGTETTLEVQ QQLGDGVVRT IAMGSTEGLK RGLTVTSTNA PISVPVGTAT LGRIMDVLGR PIDEAGPVAT EERLPIHRQA PSYAEQAAST DLLETGIKVI DLLCPFAKGG KVGLFGGAGV GKTVNMMELI NNIAKAHSGL SVFAGVGERT REGNDFYHEM KDSNVLDKVA MVYGQMNEPP GNRLRVALTG LTMAEYFRDE KDENGKGRDV LLFVDNIYRY TLAGTEVSAL LGRMPSAVGY QPTLAEEMGV LQERITSTKS GSITSIQAVY VPADDLTDPS PATTFAHLDA TVVLSRDIAS SGIYPAIDPL DSTSRQLDPL VVGQEHYEIA RAVQNVLQRY KELKDIIAIL GMDELAEEDK LVVYRARKIQ RFFSQPFHVA EVFTGAPGKL VPLKETIRGF KGLLAGEYDH IPEQAFYMVG GIDE
Validated NT Sequence
cacttcgtca ataccaccaa ccatatagaa cgcttgttct gggatgtgat cgtattcacc agctaataga cctttaaagc cacgaatcgt ttctttaagt ggtactaatt taccaggagc accagtaaat acttcagcta cgtggaatgg ttgagagaag aaacgttgga ttttacgcgc acggtaaaca accaatttat cttcttctgc caattcatcc atacccaaga ttgcgatgat gtctttaagc tctttatagc gttgcaatac gttttgtact gcgcgcgcaa tttcatagtg ctcttgacca acaactaatg ggtctaactg acgtgaagtt gagtcaagtg gatcgatcgc tggatagata ccagaagatg cgatgtcacg gctcaatact actgttgcat ctaagtgagc aaatgtagtt gcaggcgatg ggtctgttaa gtcatcggca ggtacatata ctgcttggat cgaagtgatc gaaccagact tagtagatgt aatacgctct tgaagaacac ccatttcttc tgcaagtgta ggttggtaac ctaccgcaga tggcatacga cctaacaatg ctgatacttc agtacctgca agtgtataac ggtagatgtt gtcgacgaat aataatacgt cacgaccttt accgttttcg tctttttcat cacggaagta ttcagccata gtcaaaccag ttaacgctac gcgtaaacgg ttacctggtg gctcgttcat ctgaccgtag accattgcta ctttgtcaag aacgttagaa tctttcattt cgtgatagaa gtcgttacct tcacgagtac gctcaccaac accagcaaac acagataaac ctgagtgtgc tttcgcgatg ttgttgatca attccatcat gttaacggtt ttaccaacac cagcaccacc gaataaacca actttaccac ctttcgcaaa cgggcaaagt aagtcaatta ctttaatacc agtttctaaa aggtcagtag aagctgcttg ttcagcataa gaaggcgctt gacggtgaat cggcaaacgt tcttcagtcg caacaggacc tgcttcatcg ataggacgac ctaaaacgtc catgatacgg ccaagagtgg ctgtaccaac tggaacagag atcggtgcgt ttgtgctagt tacagtaaga ccacgtttaa gaccttctgt agaacccatt gcgatggtac gaacaacacc atcaccaagt tgttgctgaa cttccaaagt agtttcagta ccgtcaactt ggagagcgtc atagatcttt ggaacgcttg tgcgctcaaa ctcgacgtcg ataaccgcgc cgatgatctg aatgatacga ccgctactca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMS SGRIIQIIGA VIDVEFERTS VPKIYDALQV DGTETTLEVQ QQLGDGVVRT IAMGSTEGLK RGLTVTSTNA PISVPVGTAT LGRIMDVLGR PIDEAGPVAT EERLPIHRQA PSYAEQAAST DLLETGIKVI DLLCPFAKGG KVGLFGGAGV GKTVNMMELI NNIAKAHSGL SVFAGVGERT REGNDFYHEM KDSNVLDKVA MVYGQMNEPP GNRLRVALTG LTMAEYFRDE KDENGKGRDV LLFVDNIYRY TLAGTEVSAL LGRMPSAVGY QPTLAEEMGV LQERITSTKS GSITSIQAVY VPADDLTDPS PATTFAHLDA TVVLSRDIAS SGIYPAIDPL DSTSRQLDPL VVGQEHYEIA RAVQNVLQRY KELKDIIAIL GMDELAEEDK LVVYRARKIQ RFFSQPFHVA EVFTGAPGKL VPLKETIRGF KGLLAGEYDH IPEQAFYMVG GIDEVIAKAE KL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta gcggtcgtat cattcagatc atcggcgcgg ttatcgacgt cgagtttgag cgcacaagcg ttccaaagat ctatgacgct ctccaagttg acggtactga aactactttg gaagttcagc aacaacttgg tgatggtgtt gttcgtacca tcgcaatggg ttctacagaa ggtcttaaac gtggtcttac tgtaactagc acaaacgcac cgatctctgt tccagttggt acagccactc ttggccgtat catggacgtt ttaggtcgtc ctatcgatga agcaggtcct gttgcgactg aagaacgttt gccgattcac cgtcaagcgc cttcttatgc tgaacaagca gcttctactg accttttaga aactggtatt aaagtaattg acttactttg cccgtttgcg aaaggtggta aagttggttt attcggtggt gctggtgttg gtaaaaccgt taacatgatg gaattgatca acaacatcgc gaaagcacac tcaggtttat ctgtgtttgc tggtgttggt gagcgtactc gtgaaggtaa cgacttctat cacgaaatga aagattctaa cgttcttgac aaagtagcaa tggtctacgg tcagatgaac gagccaccag gtaaccgttt acgcgtagcg ttaactggtt tgactatggc tgaatacttc cgtgatgaaa aagacgaaaa cggtaaaggt cgtgacgtat tattattcgt cgacaacatc taccgttata cacttgcagg tactgaagta tcagcattgt taggtcgtat gccatctgcg gtaggttacc aacctacact tgcagaagaa atgggtgttc ttcaagagcg tattacatct actaagtctg gttcgatcac ttcgatccaa gcagtatatg tacctgccga tgacttaaca gacccatcgc ctgcaactac atttgctcac ttagatgcaa cagtagtatt gagccgtgac atcgcatctt ctggtatcta tccagcgatc gatccacttg actcaacttc acgtcagtta gacccattag ttgttggtca agagcactat gaaattgcgc gcgcagtaca aaacgtattg caacgctata aagagcttaa agacatcatc gcaatcttgg gtatggatga attggcagaa gaagataaat tggttgttta ccgtgcgcgt aaaatccaac gtttcttctc tcaaccattc cacgtagctg aagtatttac tggtgctcct ggtaaattag taccacttaa agaaacgatt cgtggcttta aaggtctatt agctggtgaa tacgatcaca tcccagaaca agcgttctat atggttggtg gtattgacga agtgattgct aaagctgaga aactctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat