AcbaC.17309.a

ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family

CENTER ID: AcbaC.17309.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.17309.a.B2.GE38750 mature protein 36 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2808
GenBank: AKA32569.1
PGAT: acinetobacter:ABUW_2854
RefSeq: WP_000005666.1
UniProt: A0A0D5YKW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEVLDVVIV GGGLVGGLTA LLLAQGGVQA TVLDAAPVLD VEKTLSVMNP RVLALSQATI HLLKTVNVWD DLARQMPYTG MQVWNLNGYG EINFGHESVQ RPMPEQALGS MVEPSVLNVA IQQKMLEQLT DYRTQVRVTR IEQGVGCWHI QLADGTTLKT KLLIGADGAN SFVREQAFID LDVLDYKQAA ISCAIKTSKP HHYVARQIFL PTGPLAYLPM ASLEESENGY WQSIVWTLPD DYADEYSALS DAEFMQLLTR ESQHMLGEVL DVRSRAQFPL KARAAKQYVK AGLALIGDAA HVIHPLAGQG VNIGCLDAAV LCDALLHDLG RGVWAHEQTL MRYEHRRKGQ NDAMMHSMSA IGWLESSELF PLIWARNVGL KQAEQISFLK ERFMQQANGL GALQNTQYSR
NT Sequence
ATGAGTGAAG TGTTAGATGT CGTCATTGTT GGTGGTGGAC TAGTTGGTGG CTTAACTGCT TTATTACTTG CACAAGGTGG TGTACAGGCT ACCGTGCTTG ATGCTGCCCC TGTACTTGAT GTTGAAAAAA CATTAAGCGT GATGAACCCG CGTGTTTTAG CACTTAGCCA AGCGACCATC CATTTACTTA AAACAGTCAA TGTTTGGGAT GATCTGGCAC GTCAAATGCC TTATACAGGC ATGCAAGTGT GGAACTTAAA TGGCTATGGT GAAATTAATT TTGGCCATGA GTCTGTACAA AGACCAATGC CAGAGCAAGC CTTGGGTTCT ATGGTTGAGC CAAGTGTATT GAATGTTGCT ATTCAACAAA AAATGCTTGA GCAGTTAACA GATTATCGCA CTCAAGTTCG CGTGACTCGC ATTGAGCAGG GAGTTGGGTG TTGGCATATT CAATTGGCAG ATGGTACGAC ACTTAAAACA AAACTACTTA TTGGTGCTGA TGGCGCCAAT TCATTTGTAC GTGAGCAAGC TTTTATTGAC CTCGACGTAC TAGATTACAA GCAAGCAGCC ATTAGCTGTG CAATAAAGAC TTCCAAACCT CATCACTATG TTGCCCGTCA AATCTTCTTA CCTACAGGGC CTTTAGCATA TTTGCCAATG GCGAGTCTTG AAGAAAGTGA AAACGGCTAC TGGCAGTCCA TTGTCTGGAC ACTACCAGAT GATTATGCCG ATGAATATTC AGCTTTAAGT GATGCTGAGT TTATGCAACT TCTAACCCGT GAAAGCCAAC ATATGTTGGG TGAAGTGTTA GATGTTCGTT CAAGAGCACA ATTTCCATTG AAAGCGCGTG CCGCAAAACA ATACGTTAAA GCTGGCCTAG CCCTAATTGG CGATGCAGCT CATGTTATCC ATCCGCTTGC TGGGCAAGGG GTAAATATTG GTTGTTTAGA TGCTGCGGTA CTCTGTGATG CACTATTGCA TGATTTAGGT CGTGGCGTAT GGGCGCATGA ACAAACCTTA ATGCGTTATG AACACCGCCG TAAAGGACAA AATGATGCGA TGATGCATAG TATGTCGGCA ATTGGTTGGC TAGAAAGCAG TGAGCTATTT CCATTGATTT GGGCCCGGAA TGTGGGACTC AAGCAAGCTG AACAAATTTC GTTCTTAAAA GAGCGTTTTA TGCAACAAGC AAATGGTTTA GGGGCTTTAC AAAACACCCA GTATAGTCGT TAA
Details for AcbaC.17309.a.B2.GE38750
HARVESTED ON: 1/22/2015
SEQUENCED ON: 1/15/2015
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA PVLDVEKTLS VMNPRVLALS QATIHLLKTV NVWDDLARQM PYTGMQVWNL NGYGEINFGH ESVQRPMPEQ ALGSMVEPSV LNVAIQQKML EQLTDYRTQV RVTRIEQGVG CWHIQLADGT TLKTKLLIGA DGANSFVREQ AFIDLDVLDY KQAAISCAIK TSKPHHYVAR QIFLPTGPLA YLPMASLEES ENGYWQSIVW TLPDDYADEY SALSDAEFMQ LLTRESQHML GEVLDVRSRA QFPLKARAAK QYVKAGLALI GDAAHVIHPL AGQGVNIGCL DAAVLCDALL HDLGRGVWAH EQTLMRYEHR RKGQNDAMMH SMSAIGWLES SELFPLIWAR NVGLKQAEQI SFLKERFMQQ ANGLGAL
Validated NT Sequence
gtaaagcccc taaaccattt gcttgttgca taaaacgctc ttttaagaac gaaatttgtt cagcttgctt gagtcccaca ttccgggccc aaatcaatgg aaatagctca ctgctttcta gccaaccaat tgccgacata ctatgcatca tcgcatcatt ttgtccttta cggcggtgtt cataacgcat taaggtttgt tcatgcgccc atacgccacg acctaaatca tgcaatagtg catcacagag taccgcagca tctaaacaac caatatttac cccttgccca gcaagcggat ggataacatg agctgcatcg ccaattaggg ctaggccagc tttaacgtat tgttttgcgg cacgcgcttt caatggaaat tgtgctcttg aacgaacatc taacacttca cccaacatat gttggctttc acgggttaga agttgcataa actcagcatc acttaaagct gaatattcat cggcataatc atctggtagt gtccagacaa tggactgcca gtagccgttt tcactttctt caagactcgc cattggcaaa tatgctaaag gccctgtagg taagaagatt tgacgggcaa catagtgatg aggtttggaa gtctttattg cacagctaat ggctgcttgc ttgtaatcta gtacgtcgag gtcaataaaa gcttgctcac gtacaaatga attggcgcca tcagcaccaa taagtagttt tgttttaagt gtcgtaccat ctgccaattg aatatgccaa cacccaactc cctgctcaat gcgagtcacg cgaacttgag tgcgataatc tgttaactgc tcaagcattt tttgttgaat agcaacattc aatacacttg gctcaaccat agaacccaag gcttgctctg gcattggtct ttgtacagac tcatggccaa aattaatttc accatagcca tttaagttcc acacttgcat gcctgtataa ggcatttgac gtgccagatc atcccaaaca ttgactgttt taagtaaatg gatggtcgct tggctaagtg ctaaaacacg cgggttcatc acgcttaatg ttttttcaac atcaagtaca ggggccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHAP VLDVEKTLSV MNPRVLALSQ ATIHLLKTVN VWDDLARQMP YTGMQVWNLN GYGEINFGHE SVQRPMPEQA LGSMVEPSVL NVAIQQKMLE QLTDYRTQVR VTRIEQGVGC WHIQLADGTT LKTKLLIGAD GANSFVREQA FIDLDVLDYK QAAISCAIKT SKPHHYVARQ IFLPTGPLAY LPMASLEESE NGYWQSIVWT LPDDYADEYS ALSDAEFMQL LTRESQHMLG EVLDVRSRAQ FPLKARAAKQ YVKAGLALIG DAAHVIHPLA GQGVNIGCLD AAVLCDALLH DLGRGVWAHE QTLMRYEHRR KGQNDAMMHS MSAIGWLESS ELFPLIWARN VGLKQAEQIS FLKERFMQQA NGLGALQNTQ YSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcccctg tacttgatgt tgaaaaaaca ttaagcgtga tgaacccgcg tgttttagca cttagccaag cgaccatcca tttacttaaa acagtcaatg tttgggatga tctggcacgt caaatgcctt atacaggcat gcaagtgtgg aacttaaatg gctatggtga aattaatttt ggccatgagt ctgtacaaag accaatgcca gagcaagcct tgggttctat ggttgagcca agtgtattga atgttgctat tcaacaaaaa atgcttgagc agttaacaga ttatcgcact caagttcgcg tgactcgcat tgagcaggga gttgggtgtt ggcatattca attggcagat ggtacgacac ttaaaacaaa actacttatt ggtgctgatg gcgccaattc atttgtacgt gagcaagctt ttattgacct cgacgtacta gattacaagc aagcagccat tagctgtgca ataaagactt ccaaacctca tcactatgtt gcccgtcaaa tcttcttacc tacagggcct ttagcatatt tgccaatggc gagtcttgaa gaaagtgaaa acggctactg gcagtccatt gtctggacac taccagatga ttatgccgat gaatattcag ctttaagtga tgctgagttt atgcaacttc taacccgtga aagccaacat atgttgggtg aagtgttaga tgttcgttca agagcacaat ttccattgaa agcgcgtgcc gcaaaacaat acgttaaagc tggcctagcc ctaattggcg atgcagctca tgttatccat ccgcttgctg ggcaaggggt aaatattggt tgtttagatg ctgcggtact ctgtgatgca ctattgcatg atttaggtcg tggcgtatgg gcgcatgaac aaaccttaat gcgttatgaa caccgccgta aaggacaaaa tgatgcgatg atgcatagta tgtcggcaat tggttggcta gaaagcagtg agctatttcc attgatttgg gcccggaatg tgggactcaa gcaagctgaa caaatttcgt tcttaaaaga gcgttttatg caacaagcaa atggtttagg ggctttacaa aacacccagt atagtcgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat