AcbaC.17275.a
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
| CENTER ID: | AcbaC.17275.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17275.a.B1.GE38749 | full length | 1 | 515 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17275.a.B1.PS02383 | full length | 1 | 515 | ||
| AcbaC.17275.a.B1.PS38071 | full length | 1 | 515 |
Structures
5VPU
DEPOSITED: 5/5/2017
DETERMINATION: XRay
CLONE: AcbaC.17275.a.B1.GE38749
PROTEIN: AcbaC.17275.a.B1.PS38071
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA33328.1 |
| PATRIC ID: | fig|470.1345.peg.3570 |
| PGAT: | acinetobacter:ABUW_3643 |
| RefSeq: | WP_000128749.1 |
| UniProt: | A0A059ZPG7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDATAGKIP HVLVIMDGVG HREAIEDNAF LAAKTPNLTA MKAKHPNSLI SGSGEDVGLP DGQMGNSEVG HMNLGAGRVL YQDFTRITKD IRTGAFFEHE VLVDAVEKAK AAGGAVHIMG LLSEGGVHSH EDHIVAMCEL ALKRGAKVYL HAFLDGRDTP PRSAQPSLEK LDALFAQYEG KGRIATMIGR YFAMDRDNRW DRVEQAYRLL TEGEAVRTAT TAVEGLELAY AANENDEFVK ATRIGEIAKV QDGDSVVFMN FRADRAREIT RAFVEKDFAG FERKVVPNLS KFVMLTRYQA SIDAPVAYMP EELKNSLGEY LSSLGKTQLR IAETEKYAHV TFFFSGGRED EYPGEKRILI PSPNVATYDL KPEMSAYEVT DELVKAINSG EYDLLVVNYA NGDMVGHTGV FDAAVKAVEA VDTCLGRVYE AVMAKKGHML ITADHGNVEQ MQDYESGQVH TQHTTELVPF IYVGPTQATI AEGGVLADVA PTILNLMQIP VPAEMQGRNL ITLSA
NT Sequence
ATGACGGATG CAACTGCTGG CAAGATCCCT CACGTTCTGG TCATTATGGA TGGTGTTGGA CACCGTGAAG CGATTGAAGA TAATGCTTTT CTTGCAGCAA AAACCCCGAA TTTAACTGCA ATGAAAGCAA AGCATCCAAA TAGTCTCATT TCTGGTTCTG GTGAAGATGT TGGTTTACCT GATGGACAAA TGGGTAACTC TGAAGTTGGT CACATGAACC TTGGCGCTGG CCGAGTGTTA TACCAAGACT TCACACGTAT TACTAAAGAT ATTCGTACTG GTGCTTTTTT TGAACACGAA GTATTGGTTG ATGCCGTAGA AAAAGCAAAA GCAGCGGGTG GCGCAGTTCA TATTATGGGC CTACTCTCTG AAGGCGGTGT TCACTCACAT GAAGATCATA TTGTGGCGAT GTGTGAACTT GCCTTAAAAC GCGGTGCGAA AGTTTACTTA CATGCATTCC TTGATGGTCG TGATACTCCT CCACGTAGTG CTCAACCTTC ATTAGAAAAA TTAGATGCTT TATTCGCTCA ATATGAAGGC AAGGGCCGTA TTGCAACCAT GATTGGTCGC TATTTTGCAA TGGATCGTGA TAACCGTTGG GATCGTGTTG AGCAGGCTTA CCGTTTATTA ACGGAAGGTG AAGCTGTTCG TACTGCAACT ACTGCGGTTG AAGGCTTAGA GCTTGCATAT GCTGCAAATG AAAATGATGA GTTTGTAAAA GCAACCCGTA TTGGTGAGAT CGCTAAAGTT CAAGATGGCG ATAGCGTTGT ATTTATGAAT TTCCGTGCTG ACCGTGCCCG TGAAATTACG CGTGCGTTTG TTGAAAAAGA TTTCGCAGGT TTTGAGCGTA AAGTTGTACC GAACCTTTCT AAATTTGTCA TGTTGACGCG TTATCAGGCA AGTATTGATG CACCTGTGGC ATATATGCCT GAAGAGTTAA AGAACTCGCT TGGTGAATAT TTATCTTCTT TAGGTAAAAC GCAATTACGT ATTGCTGAAA CTGAGAAATA TGCCCATGTG ACTTTCTTCT TTAGTGGTGG TCGTGAAGAC GAATATCCAG GTGAAAAGCG TATTTTAATT CCATCGCCAA ACGTTGCGAC ATATGACTTA AAGCCTGAAA TGAGTGCTTA TGAAGTAACT GATGAGCTAG TCAAAGCAAT TAACTCGGGT GAGTACGACT TACTTGTTGT GAACTATGCC AATGGTGATA TGGTCGGTCA TACAGGCGTA TTTGATGCTG CAGTTAAGGC TGTTGAAGCA GTTGATACAT GTTTAGGTCG TGTATACGAA GCTGTAATGG CGAAAAAAGG CCATATGCTT ATTACGGCTG ACCACGGTAA TGTTGAACAA ATGCAAGATT ATGAAAGTGG TCAGGTTCAT ACTCAGCATA CAACAGAGCT TGTGCCTTTT ATTTATGTGG GTCCAACCCA AGCAACGATT GCAGAAGGTG GCGTGCTTGC TGATGTTGCA CCGACCATTC TGAACTTAAT GCAGATTCCT GTGCCTGCTG AAATGCAAGG TCGTAATCTC ATTACATTGT CTGCTTAA
Details for AcbaC.17275.a.B1.GE38749
| HARVESTED ON: | 1/22/2015 |
| SEQUENCED ON: | 1/15/2015 |
| EXPECTED MW: | 57kDa |
| OBSERVED MW: | 57kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMT DATAGKIPHV LVIMDGVGHR EAIEDNAFLA AKTPNLTAMK AKHPNSLISG SGEDVGLPDG QMGNSEVGHM NLGAGRVLYQ DFTRITKDIR TGAFFEHEVL VDAVEKAKAA GGAVHIMGLL SEGGVHSHED HIVAMCELAL KRGAKVYLHA FLDGRDTPPR SAQPSLEKLD ALFAQYEGKG RIATMIGRYF AMDRDNRWDR VEQAYRLLTE GEAVRTATTA VEGLELAYAA NENDEFVKAT RIGEIAKVQD GDSVVFMNFR ADRAREITRA FVEKDFAGFE RKVVPNLSKF VMLTRYQASI DAPVAYMPEE LKNSLGEYLS SLGKTQLRIA ETEKYAHVTF FFSGGREDEY PGEKRILIPS PNVATYDLKP EMSAYEVTDE LVKAINSGEY DLLVVNYANG DMVGHTGVFD AAVKAVEAVD TCLGRVYEAV MAKKGHMLIT ADHGNVEQMQ DYESGQVHTQ HTTELVPFIY VGPTQATIAE GGVLADVAPT ILNLMQIPVP AEMQG
Validated NT Sequence
acgaccttgc atttcagcag gcacaggaat ctgcattaag ttcagaatgg tcggtgcaac atcagcaagc acgccacctt ctgcaatcgt tgcttgggtt ggacccacat aaataaaagg cacaagctct gttgtatgct gagtatgaac ctgaccactt tcataatctt gcatttgttc aacattaccg tggtcagccg taataagcat atggcctttt ttcgccatta cagcttcgta tacacgacct aaacatgtat caactgcttc aacagcctta actgcagcat caaatacgcc tgtatgaccg accatatcac cattggcata gttcacaaca agtaagtcgt actcacccga gttaattgct ttgactagct catcagttac ttcataagca ctcatttcag gctttaagtc atatgtcgca acgtttggcg atggaattaa aatacgcttt tcacctggat attcgtcttc acgaccacca ctaaagaaga aagtcacatg ggcatatttc tcagtttcag caatacgtaa ttgcgtttta cctaaagaag ataaatattc accaagcgag ttctttaact cttcaggcat atatgccaca ggtgcatcaa tacttgcctg ataacgcgtc aacatgacaa atttagaaag gttcggtaca actttacgct caaaacctgc gaaatctttt tcaacaaacg cacgcgtaat ttcacgggca cggtcagcac ggaaattcat aaatacaacg ctatcgccat cttgaacttt agcgatctca ccaatacggg ttgcttttac aaactcatca ttttcatttg cagcatatgc aagctctaag ccttcaaccg cagtagttgc agtacgaaca gcttcacctt ccgttaataa acggtaagcc tgctcaacac gatcccaacg gttatcacga tccattgcaa aatagcgacc aatcatggtt gcaatacggc ccttgccttc atattgagcg aataaagcat ctaatttttc taatgaaggt tgagcactac gtggaggagt atcacgacca tcaaggaatg catgtaagta aactttcgca ccgcgtttta aggcaagttc acacatcgcc acaatatgat cttcatgtga gtgaacaccg ccttcagaga gtaggcccat aatatgaact gcgccacccg ctgcttttgc tttttctacg gcatcaacca atacttcgtg ttcaaaaaaa gcaccagtac gaatatcttt agtaatacgt gtgaagtctt ggtataacac tcggccagcg ccaaggttca tgtgaccaac ttcagagtta cccatttgtc catcaggtaa accaacatct tcaccagaac cagaaatgag actatttgga tgctttgctt tcattgcagt taaattcggg gtttttgctg caagaaaagc attatcttca atcgcttcac ggtgtccaac accatccata atgaccagaa cgtgagggat cttgccagca gttgcatccg tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMT DATAGKIPHV LVIMDGVGHR EAIEDNAFLA AKTPNLTAMK AKHPNSLISG SGEDVGLPDG QMGNSEVGHM NLGAGRVLYQ DFTRITKDIR TGAFFEHEVL VDAVEKAKAA GGAVHIMGLL SEGGVHSHED HIVAMCELAL KRGAKVYLHA FLDGRDTPPR SAQPSLEKLD ALFAQYEGKG RIATMIGRYF AMDRDNRWDR VEQAYRLLTE GEAVRTATTA VEGLELAYAA NENDEFVKAT RIGEIAKVQD GDSVVFMNFR ADRAREITRA FVEKDFAGFE RKVVPNLSKF VMLTRYQASI DAPVAYMPEE LKNSLGEYLS SLGKTQLRIA ETEKYAHVTF FFSGGREDEY PGEKRILIPS PNVATYDLKP EMSAYEVTDE LVKAINSGEY DLLVVNYANG DMVGHTGVFD AAVKAVEAVD TCLGRVYEAV MAKKGHMLIT ADHGNVEQMQ DYESGQVHTQ HTTELVPFIY VGPTQATIAE GGVLADVAPT ILNLMQIPVP AEMQGRNLIT LSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg atgcaactgc tggcaagatc cctcacgttc tggtcattat ggatggtgtt ggacaccgtg aagcgattga agataatgct tttcttgcag caaaaacccc gaatttaact gcaatgaaag caaagcatcc aaatagtctc atttctggtt ctggtgaaga tgttggttta cctgatggac aaatgggtaa ctctgaagtt ggtcacatga accttggcgc tggccgagtg ttataccaag acttcacacg tattactaaa gatattcgta ctggtgcttt ttttgaacac gaagtattgg ttgatgccgt agaaaaagca aaagcagcgg gtggcgcagt tcatattatg ggcctactct ctgaaggcgg tgttcactca catgaagatc atattgtggc gatgtgtgaa cttgccttaa aacgcggtgc gaaagtttac ttacatgcat tccttgatgg tcgtgatact cctccacgta gtgctcaacc ttcattagaa aaattagatg ctttattcgc tcaatatgaa ggcaagggcc gtattgcaac catgattggt cgctattttg caatggatcg tgataaccgt tgggatcgtg ttgagcaggc ttaccgttta ttaacggaag gtgaagctgt tcgtactgca actactgcgg ttgaaggctt agagcttgca tatgctgcaa atgaaaatga tgagtttgta aaagcaaccc gtattggtga gatcgctaaa gttcaagatg gcgatagcgt tgtatttatg aatttccgtg ctgaccgtgc ccgtgaaatt acgcgtgcgt ttgttgaaaa agatttcgca ggttttgagc gtaaagttgt accgaacctt tctaaatttg tcatgttgac gcgttatcag gcaagtattg atgcacctgt ggcatatatg cctgaagagt taaagaactc gcttggtgaa tatttatctt ctttaggtaa aacgcaatta cgtattgctg aaactgagaa atatgcccat gtgactttct tctttagtgg tggtcgtgaa gacgaatatc caggtgaaaa gcgtatttta attccatcgc caaacgttgc gacatatgac ttaaagcctg aaatgagtgc ttatgaagta actgatgagc tagtcaaagc aattaactcg ggtgagtacg acttacttgt tgtgaactat gccaatggtg atatggtcgg tcatacaggc gtatttgatg ctgcagttaa ggctgttgaa gcagttgata catgtttagg tcgtgtatac gaagctgtaa tggcgaaaaa aggccatatg cttattacgg ctgaccacgg taatgttgaa caaatgcaag attatgaaag tggtcaggtt catactcagc atacaacaga gcttgtgcct tttatttatg tgggtccaac ccaagcaacg attgcagaag gtggcgtgct tgctgatgtt gcaccgacca ttctgaactt aatgcagatt cctgtgcctg ctgaaatgca aggtcgtaat ctcattacat tgtctgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for AcbaC.17275.a.B1.PS02383
| PURIFICATION DATe: | 4/21/2015 |
| CONCENTRATION: | 29mg/ml |
| OBSERVED MW: | 62kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT DATAGKIPHV LVIMDGVGHR EAIEDNAFLA AKTPNLTAMK AKHPNSLISG SGEDVGLPDG QMGNSEVGHM NLGAGRVLYQ DFTRITKDIR TGAFFEHEVL VDAVEKAKAA GGAVHIMGLL SEGGVHSHED HIVAMCELAL KRGAKVYLHA FLDGRDTPPR SAQPSLEKLD ALFAQYEGKG RIATMIGRYF AMDRDNRWDR VEQAYRLLTE GEAVRTATTA VEGLELAYAA NENDEFVKAT RIGEIAKVQD GDSVVFMNFR ADRAREITRA FVEKDFAGFE RKVVPNLSKF VMLTRYQASI DAPVAYMPEE LKNSLGEYLS SLGKTQLRIA ETEKYAHVTF FFSGGREDEY PGEKRILIPS PNVATYDLKP EMSAYEVTDE LVKAINSGEY DLLVVNYANG DMVGHTGVFD AAVKAVEAVD TCLGRVYEAV MAKKGHMLIT ADHGNVEQMQ DYESGQVHTQ HTTELVPFIY VGPTQATIAE GGVLADVAPT ILNLMQIPVP AEMQG
Validated NT Sequence
acgaccttgc atttcagcag gcacaggaat ctgcattaag ttcagaatgg tcggtgcaac atcagcaagc acgccacctt ctgcaatcgt tgcttgggtt ggacccacat aaataaaagg cacaagctct gttgtatgct gagtatgaac ctgaccactt tcataatctt gcatttgttc aacattaccg tggtcagccg taataagcat atggcctttt ttcgccatta cagcttcgta tacacgacct aaacatgtat caactgcttc aacagcctta actgcagcat caaatacgcc tgtatgaccg accatatcac cattggcata gttcacaaca agtaagtcgt actcacccga gttaattgct ttgactagct catcagttac ttcataagca ctcatttcag gctttaagtc atatgtcgca acgtttggcg atggaattaa aatacgcttt tcacctggat attcgtcttc acgaccacca ctaaagaaga aagtcacatg ggcatatttc tcagtttcag caatacgtaa ttgcgtttta cctaaagaag ataaatattc accaagcgag ttctttaact cttcaggcat atatgccaca ggtgcatcaa tacttgcctg ataacgcgtc aacatgacaa atttagaaag gttcggtaca actttacgct caaaacctgc gaaatctttt tcaacaaacg cacgcgtaat ttcacgggca cggtcagcac ggaaattcat aaatacaacg ctatcgccat cttgaacttt agcgatctca ccaatacggg ttgcttttac aaactcatca ttttcatttg cagcatatgc aagctctaag ccttcaaccg cagtagttgc agtacgaaca gcttcacctt ccgttaataa acggtaagcc tgctcaacac gatcccaacg gttatcacga tccattgcaa aatagcgacc aatcatggtt gcaatacggc ccttgccttc atattgagcg aataaagcat ctaatttttc taatgaaggt tgagcactac gtggaggagt atcacgacca tcaaggaatg catgtaagta aactttcgca ccgcgtttta aggcaagttc acacatcgcc acaatatgat cttcatgtga gtgaacaccg ccttcagaga gtaggcccat aatatgaact gcgccacccg ctgcttttgc tttttctacg gcatcaacca atacttcgtg ttcaaaaaaa gcaccagtac gaatatcttt agtaatacgt gtgaagtctt ggtataacac tcggccagcg ccaaggttca tgtgaccaac ttcagagtta cccatttgtc catcaggtaa accaacatct tcaccagaac cagaaatgag actatttgga tgctttgctt tcattgcagt taaattcggg gtttttgctg caagaaaagc attatcttca atcgcttcac ggtgtccaac accatccata atgaccagaa cgtgagggat cttgccagca gttgcatccg tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMT DATAGKIPHV LVIMDGVGHR EAIEDNAFLA AKTPNLTAMK AKHPNSLISG SGEDVGLPDG QMGNSEVGHM NLGAGRVLYQ DFTRITKDIR TGAFFEHEVL VDAVEKAKAA GGAVHIMGLL SEGGVHSHED HIVAMCELAL KRGAKVYLHA FLDGRDTPPR SAQPSLEKLD ALFAQYEGKG RIATMIGRYF AMDRDNRWDR VEQAYRLLTE GEAVRTATTA VEGLELAYAA NENDEFVKAT RIGEIAKVQD GDSVVFMNFR ADRAREITRA FVEKDFAGFE RKVVPNLSKF VMLTRYQASI DAPVAYMPEE LKNSLGEYLS SLGKTQLRIA ETEKYAHVTF FFSGGREDEY PGEKRILIPS PNVATYDLKP EMSAYEVTDE LVKAINSGEY DLLVVNYANG DMVGHTGVFD AAVKAVEAVD TCLGRVYEAV MAKKGHMLIT ADHGNVEQMQ DYESGQVHTQ HTTELVPFIY VGPTQATIAE GGVLADVAPT ILNLMQIPVP AEMQGRNLIT LSA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGG ATGCAACTGC TGGCAAGATC CCTCACGTTC TGGTCATTAT GGATGGTGTT GGACACCGTG AAGCGATTGA AGATAATGCT TTTCTTGCAG CAAAAACCCC GAATTTAACT GCAATGAAAG CAAAGCATCC AAATAGTCTC ATTTCTGGTT CTGGTGAAGA TGTTGGTTTA CCTGATGGAC AAATGGGTAA CTCTGAAGTT GGTCACATGA ACCTTGGCGC TGGCCGAGTG TTATACCAAG ACTTCACACG TATTACTAAA GATATTCGTA CTGGTGCTTT TTTTGAACAC GAAGTATTGG TTGATGCCGT AGAAAAAGCA AAAGCAGCGG GTGGCGCAGT TCATATTATG GGCCTACTCT CTGAAGGCGG TGTTCACTCA CATGAAGATC ATATTGTGGC GATGTGTGAA CTTGCCTTAA AACGCGGTGC GAAAGTTTAC TTACATGCAT TCCTTGATGG TCGTGATACT CCTCCACGTA GTGCTCAACC TTCATTAGAA AAATTAGATG CTTTATTCGC TCAATATGAA GGCAAGGGCC GTATTGCAAC CATGATTGGT CGCTATTTTG CAATGGATCG TGATAACCGT TGGGATCGTG TTGAGCAGGC TTACCGTTTA TTAACGGAAG GTGAAGCTGT TCGTACTGCA ACTACTGCGG TTGAAGGCTT AGAGCTTGCA TATGCTGCAA ATGAAAATGA TGAGTTTGTA AAAGCAACCC GTATTGGTGA GATCGCTAAA GTTCAAGATG GCGATAGCGT TGTATTTATG AATTTCCGTG CTGACCGTGC CCGTGAAATT ACGCGTGCGT TTGTTGAAAA AGATTTCGCA GGTTTTGAGC GTAAAGTTGT ACCGAACCTT TCTAAATTTG TCATGTTGAC GCGTTATCAG GCAAGTATTG ATGCACCTGT GGCATATATG CCTGAAGAGT TAAAGAACTC GCTTGGTGAA TATTTATCTT CTTTAGGTAA AACGCAATTA CGTATTGCTG AAACTGAGAA ATATGCCCAT GTGACTTTCT TCTTTAGTGG TGGTCGTGAA GACGAATATC CAGGTGAAAA GCGTATTTTA ATTCCATCGC CAAACGTTGC GACATATGAC TTAAAGCCTG AAATGAGTGC TTATGAAGTA ACTGATGAGC TAGTCAAAGC AATTAACTCG GGTGAGTACG ACTTACTTGT TGTGAACTAT GCCAATGGTG ATATGGTCGG TCATACAGGC GTATTTGATG CTGCAGTTAA GGCTGTTGAA GCAGTTGATA CATGTTTAGG TCGTGTATAC GAAGCTGTAA TGGCGAAAAA AGGCCATATG CTTATTACGG CTGACCACGG TAATGTTGAA CAAATGCAAG ATTATGAAAG TGGTCAGGTT CATACTCAGC ATACAACAGA GCTTGTGCCT TTTATTTATG TGGGTCCAAC CCAAGCAACG ATTGCAGAAG GTGGCGTGCT TGCTGATGTT GCACCGACCA TTCTGAACTT AATGCAGATT CCTGTGCCTG CTGAAATGCA AGGTCGTAAT CTCATTACAT TGTCTGCTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT
Details for AcbaC.17275.a.B1.PS38071
| PURIFICATION DATe: | 10/11/2016 |
| CONCENTRATION: | 24.7mg/ml |
| OBSERVED MW: | 68kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT DATAGKIPHV LVIMDGVGHR EAIEDNAFLA AKTPNLTAMK AKHPNSLISG SGEDVGLPDG QMGNSEVGHM NLGAGRVLYQ DFTRITKDIR TGAFFEHEVL VDAVEKAKAA GGAVHIMGLL SEGGVHSHED HIVAMCELAL KRGAKVYLHA FLDGRDTPPR SAQPSLEKLD ALFAQYEGKG RIATMIGRYF AMDRDNRWDR VEQAYRLLTE GEAVRTATTA VEGLELAYAA NENDEFVKAT RIGEIAKVQD GDSVVFMNFR ADRAREITRA FVEKDFAGFE RKVVPNLSKF VMLTRYQASI DAPVAYMPEE LKNSLGEYLS SLGKTQLRIA ETEKYAHVTF FFSGGREDEY PGEKRILIPS PNVATYDLKP EMSAYEVTDE LVKAINSGEY DLLVVNYANG DMVGHTGVFD AAVKAVEAVD TCLGRVYEAV MAKKGHMLIT ADHGNVEQMQ DYESGQVHTQ HTTELVPFIY VGPTQATIAE GGVLADVAPT ILNLMQIPVP AEMQG
Validated NT Sequence
acgaccttgc atttcagcag gcacaggaat ctgcattaag ttcagaatgg tcggtgcaac atcagcaagc acgccacctt ctgcaatcgt tgcttgggtt ggacccacat aaataaaagg cacaagctct gttgtatgct gagtatgaac ctgaccactt tcataatctt gcatttgttc aacattaccg tggtcagccg taataagcat atggcctttt ttcgccatta cagcttcgta tacacgacct aaacatgtat caactgcttc aacagcctta actgcagcat caaatacgcc tgtatgaccg accatatcac cattggcata gttcacaaca agtaagtcgt actcacccga gttaattgct ttgactagct catcagttac ttcataagca ctcatttcag gctttaagtc atatgtcgca acgtttggcg atggaattaa aatacgcttt tcacctggat attcgtcttc acgaccacca ctaaagaaga aagtcacatg ggcatatttc tcagtttcag caatacgtaa ttgcgtttta cctaaagaag ataaatattc accaagcgag ttctttaact cttcaggcat atatgccaca ggtgcatcaa tacttgcctg ataacgcgtc aacatgacaa atttagaaag gttcggtaca actttacgct caaaacctgc gaaatctttt tcaacaaacg cacgcgtaat ttcacgggca cggtcagcac ggaaattcat aaatacaacg ctatcgccat cttgaacttt agcgatctca ccaatacggg ttgcttttac aaactcatca ttttcatttg cagcatatgc aagctctaag ccttcaaccg cagtagttgc agtacgaaca gcttcacctt ccgttaataa acggtaagcc tgctcaacac gatcccaacg gttatcacga tccattgcaa aatagcgacc aatcatggtt gcaatacggc ccttgccttc atattgagcg aataaagcat ctaatttttc taatgaaggt tgagcactac gtggaggagt atcacgacca tcaaggaatg catgtaagta aactttcgca ccgcgtttta aggcaagttc acacatcgcc acaatatgat cttcatgtga gtgaacaccg ccttcagaga gtaggcccat aatatgaact gcgccacccg ctgcttttgc tttttctacg gcatcaacca atacttcgtg ttcaaaaaaa gcaccagtac gaatatcttt agtaatacgt gtgaagtctt ggtataacac tcggccagcg ccaaggttca tgtgaccaac ttcagagtta cccatttgtc catcaggtaa accaacatct tcaccagaac cagaaatgag actatttgga tgctttgctt tcattgcagt taaattcggg gtttttgctg caagaaaagc attatcttca atcgcttcac ggtgtccaac accatccata atgaccagaa cgtgagggat cttgccagca gttgcatccg tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMT DATAGKIPHV LVIMDGVGHR EAIEDNAFLA AKTPNLTAMK AKHPNSLISG SGEDVGLPDG QMGNSEVGHM NLGAGRVLYQ DFTRITKDIR TGAFFEHEVL VDAVEKAKAA GGAVHIMGLL SEGGVHSHED HIVAMCELAL KRGAKVYLHA FLDGRDTPPR SAQPSLEKLD ALFAQYEGKG RIATMIGRYF AMDRDNRWDR VEQAYRLLTE GEAVRTATTA VEGLELAYAA NENDEFVKAT RIGEIAKVQD GDSVVFMNFR ADRAREITRA FVEKDFAGFE RKVVPNLSKF VMLTRYQASI DAPVAYMPEE LKNSLGEYLS SLGKTQLRIA ETEKYAHVTF FFSGGREDEY PGEKRILIPS PNVATYDLKP EMSAYEVTDE LVKAINSGEY DLLVVNYANG DMVGHTGVFD AAVKAVEAVD TCLGRVYEAV MAKKGHMLIT ADHGNVEQMQ DYESGQVHTQ HTTELVPFIY VGPTQATIAE GGVLADVAPT ILNLMQIPVP AEMQGRNLIT LSA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGG ATGCAACTGC TGGCAAGATC CCTCACGTTC TGGTCATTAT GGATGGTGTT GGACACCGTG AAGCGATTGA AGATAATGCT TTTCTTGCAG CAAAAACCCC GAATTTAACT GCAATGAAAG CAAAGCATCC AAATAGTCTC ATTTCTGGTT CTGGTGAAGA TGTTGGTTTA CCTGATGGAC AAATGGGTAA CTCTGAAGTT GGTCACATGA ACCTTGGCGC TGGCCGAGTG TTATACCAAG ACTTCACACG TATTACTAAA GATATTCGTA CTGGTGCTTT TTTTGAACAC GAAGTATTGG TTGATGCCGT AGAAAAAGCA AAAGCAGCGG GTGGCGCAGT TCATATTATG GGCCTACTCT CTGAAGGCGG TGTTCACTCA CATGAAGATC ATATTGTGGC GATGTGTGAA CTTGCCTTAA AACGCGGTGC GAAAGTTTAC TTACATGCAT TCCTTGATGG TCGTGATACT CCTCCACGTA GTGCTCAACC TTCATTAGAA AAATTAGATG CTTTATTCGC TCAATATGAA GGCAAGGGCC GTATTGCAAC CATGATTGGT CGCTATTTTG CAATGGATCG TGATAACCGT TGGGATCGTG TTGAGCAGGC TTACCGTTTA TTAACGGAAG GTGAAGCTGT TCGTACTGCA ACTACTGCGG TTGAAGGCTT AGAGCTTGCA TATGCTGCAA ATGAAAATGA TGAGTTTGTA AAAGCAACCC GTATTGGTGA GATCGCTAAA GTTCAAGATG GCGATAGCGT TGTATTTATG AATTTCCGTG CTGACCGTGC CCGTGAAATT ACGCGTGCGT TTGTTGAAAA AGATTTCGCA GGTTTTGAGC GTAAAGTTGT ACCGAACCTT TCTAAATTTG TCATGTTGAC GCGTTATCAG GCAAGTATTG ATGCACCTGT GGCATATATG CCTGAAGAGT TAAAGAACTC GCTTGGTGAA TATTTATCTT CTTTAGGTAA AACGCAATTA CGTATTGCTG AAACTGAGAA ATATGCCCAT GTGACTTTCT TCTTTAGTGG TGGTCGTGAA GACGAATATC CAGGTGAAAA GCGTATTTTA ATTCCATCGC CAAACGTTGC GACATATGAC TTAAAGCCTG AAATGAGTGC TTATGAAGTA ACTGATGAGC TAGTCAAAGC AATTAACTCG GGTGAGTACG ACTTACTTGT TGTGAACTAT GCCAATGGTG ATATGGTCGG TCATACAGGC GTATTTGATG CTGCAGTTAA GGCTGTTGAA GCAGTTGATA CATGTTTAGG TCGTGTATAC GAAGCTGTAA TGGCGAAAAA AGGCCATATG CTTATTACGG CTGACCACGG TAATGTTGAA CAAATGCAAG ATTATGAAAG TGGTCAGGTT CATACTCAGC ATACAACAGA GCTTGTGCCT TTTATTTATG TGGGTCCAAC CCAAGCAACG ATTGCAGAAG GTGGCGTGCT TGCTGATGTT GCACCGACCA TTCTGAACTT AATGCAGATT CCTGTGCCTG CTGAAATGCA AGGTCGTAAT CTCATTACAT TGTCTGCTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT