SSGCID: Target Page

AcbaC.17231.a

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

CENTER ID:	AcbaC.17231.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17231.a.B1.GE38746	full length		1	371

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.17231.a.B1.PS38072	full length		1	371

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3312
GenBank:	AKA33073.1
PGAT:	acinetobacter:ABUW_3377
RefSeq:	WP_000572095.1
UniProt:	V5VGK9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIENPIKRRP TRKIRVGSVY VGGDAPISVQ SMTNTETCDV DATVAQIERC VDAGADIMRV SVPSMEAAEA FGAIRKRVSV PLVADIHFDH RIALAVADYG ADCLRINPGN IGSDQKVREV VAAARHHGIS MRIGVNAGSL EKDLQKKYGE PTGQALLESA LRHIDILDRL DFHEFKVSVK ASNVFLTMDA YRLLSQQIDN PLHLGVTEAG IYRTGTVKSA IALGGLLMEG IGDTMRISLA AEPEDEIKIG FDILKSLGLR SNGINFIACP SCSRQEFNVI QVMQALEERL EDIRTPMDVS VIGCKVNGPG EAKEADIGVV GAAPRSLVYR NGEKSHLIDT NQLVDEIETM VRQRVQELEE AKSKEIIRSS S

NT Sequence

ATGATAGAGA ACCCAATTAA ACGTCGACCA ACACGTAAAA TCCGTGTTGG TTCGGTCTAT GTCGGTGGCG ATGCACCTAT TAGTGTGCAA AGTATGACAA ATACCGAAAC TTGCGATGTT GACGCAACTG TGGCTCAGAT TGAGCGCTGT GTTGATGCAG GTGCAGATAT TATGCGTGTT TCAGTGCCTT CTATGGAAGC TGCTGAAGCG TTTGGTGCAA TCCGTAAGCG TGTTTCAGTT CCATTAGTTG CTGATATCCA TTTTGACCAT AGAATTGCTT TGGCAGTTGC AGATTATGGT GCGGACTGCT TGCGTATTAA CCCGGGCAAT ATCGGTTCAG ACCAGAAAGT TCGTGAAGTT GTGGCTGCGG CACGTCATCA CGGTATTTCT ATGCGTATTG GCGTGAACGC GGGTTCTCTA GAAAAAGATT TACAGAAAAA ATATGGCGAG CCTACAGGGC AGGCACTTCT TGAGTCAGCT TTACGTCATA TTGATATTTT AGACCGTCTT GACTTCCATG AGTTTAAAGT CAGCGTAAAA GCATCAAATG TGTTTTTAAC TATGGATGCT TATCGTTTAC TTTCTCAACA AATTGATAAT CCATTACACC TTGGAGTGAC TGAAGCTGGT ATTTACCGTA CAGGTACTGT GAAATCAGCG ATTGCTCTTG GTGGGTTATT AATGGAAGGC ATTGGCGATA CGATGCGTAT TTCGCTTGCT GCTGAACCTG AAGATGAAAT CAAGATCGGT TTTGATATCT TAAAATCGCT TGGCCTACGT TCTAACGGTA TTAACTTTAT TGCTTGTCCA AGTTGTTCTC GCCAAGAATT TAACGTGATT CAGGTGATGC AGGCTTTAGA AGAGCGTTTA GAAGATATCC GTACACCAAT GGACGTTTCG GTTATTGGCT GTAAGGTAAA TGGCCCAGGT GAAGCAAAAG AAGCAGATAT TGGGGTCGTT GGGGCTGCGC CTCGTTCATT GGTTTATCGT AATGGTGAGA AAAGCCATTT AATTGATACC AATCAATTGG TTGATGAAAT CGAAACTATG GTTCGTCAAC GTGTTCAAGA GCTTGAAGAA GCTAAATCTA AAGAAATTAT TCGTAGTTCA TCATGA

Details for AcbaC.17231.a.B1.GE38746

HARVESTED ON:	1/22/2015
SEQUENCED ON:	1/15/2015
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMI ENPIKRRPTR KIRVGSVYVG GDAPISVQSM TNTETCDVDA TVAQIERCVD AGADIMRVSV PSMEAAEAFG AIRKRVSVPL VADIHFDHRI ALAVADYGAD CLRINPGNIG SDQKVREVVA AARHHGISMR IGVNAGSLEK DLQKKYGEPT GQALLESALR HIDILDRLDF HEFKVSVKAS NVFLTMDAYR LLSQQIDNPL HLGVTEAGIY RTGTVKSAIA LGGLLMEGIG DTMRISLAAE PEDEIKIGFD ILKSLGLRSN GINFIACPSC SRQEFNVIQV MQALEERLED IRTPMDVSVI GCKVNGPGEA KEADIGVVGA APRSLVYRNG EKSHLIDTNQ LVDEIETMVR QRVQELEEAK S

Validated NT Sequence

tttagattta gcttcttcaa gctcttgaac acgttgacga accatagttt cgatttcatc aaccaattga ttggtatcaa ttaaatggct tttctcacca ttacgataaa ccaatgaacg aggcgcagcc ccaacgaccc caatatctgc ttcttttgct tcacctgggc catttacctt acagccaata accgaaacgt ccattggtgt acggatatct tctaaacgct cttctaaagc ctgcatcacc tgaatcacgt taaattcttg gcgagaacaa cttggacaag caataaagtt aataccgtta gaacgtaggc caagcgattt taagatatca aaaccgatct tgatttcatc ttcaggttca gcagcaagcg aaatacgcat cgtatcgcca atgccttcca ttaataaccc accaagagca atcgctgatt tcacagtacc tgtacggtaa ataccagctt cagtcactcc aaggtgtaat ggattatcaa tttgttgaga aagtaaacga taagcatcca tagttaaaaa cacatttgat gcttttacgc tgactttaaa ctcatggaag tcaagacggt ctaaaatatc aatatgacgt aaagctgact caagaagtgc ctgccctgta ggctcgccat attttttctg taaatctttt tctagagaac ccgcgttcac gccaatacgc atagaaatac cgtgatgacg tgccgcagcc acaacttcac gaactttctg gtctgaaccg atattgcccg ggttaatacg caagcagtcc gcaccataat ctgcaactgc caaagcaatt ctatggtcaa aatggatatc agcaactaat ggaactgaaa cacgcttacg gattgcacca aacgcttcag cagcttccat agaaggcact gaaacacgca taatatctgc acctgcatca acacagcgct caatctgagc cacagttgcg tcaacatcgc aagtttcggt atttgtcata ctttgcacac taataggtgc atcgccaccg acatagaccg aaccaacacg gattttacgt gttggtcgac gtttaattgg gttctctatc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatag agaacccaat taaacgtcga ccaacacgta aaatccgtgt tggttcggtc tatgtcggtg gcgatgcacc tattagtgtg caaagtatga caaataccga aacttgcgat gttgacgcaa ctgtggctca gattgagcgc tgtgttgatg caggtgcaga tattatgcgt gtttcagtgc cttctatgga agctgctgaa gcgtttggtg caatccgtaa gcgtgtttca gttccattag ttgctgatat ccattttgac catagaattg ctttggcagt tgcagattat ggtgcggact gcttgcgtat taacccgggc aatatcggtt cagaccagaa agttcgtgaa gttgtggctg cggcacgtca tcacggtatt tctatgcgta ttggcgtgaa cgcgggttct ctagaaaaag atttacagaa aaaatatggc gagcctacag ggcaggcact tcttgagtca gctttacgtc atattgatat tttagaccgt cttgacttcc atgagtttaa agtcagcgta aaagcatcaa atgtgttttt aactatggat gcttatcgtt tactttctca acaaattgat aatccattac accttggagt gactgaagct ggtatttacc gtacaggtac tgtgaaatca gcgattgctc ttggtgggtt attaatggaa ggcattggcg atacgatgcg tatttcgctt gctgctgaac ctgaagatga aatcaagatc ggttttgata tcttaaaatc gcttggccta cgttctaacg gtattaactt tattgcttgt ccaagttgtt ctcgccaaga atttaacgtg attcaggtga tgcaggcttt agaagagcgt ttagaagata tccgtacacc aatggacgtt tcggttattg gctgtaaggt aaatggccca ggtgaagcaa aagaagcaga tattggggtc gttggggctg cgcctcgttc attggtttat cgtaatggtg agaaaagcca tttaattgat accaatcaat tggttgatga aatcgaaact atggttcgtc aacgtgttca agagcttgaa gaagctaaat ctaaagaaat tattcgtagt tcatcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for AcbaC.17231.a.B1.PS38072

PURIFICATION DATe:	10/12/2016
CONCENTRATION:	31.2mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAG AGAACCCAAT TAAACGTCGA CCAACACGTA AAATCCGTGT TGGTTCGGTC TATGTCGGTG GCGATGCACC TATTAGTGTG CAAAGTATGA CAAATACCGA AACTTGCGAT GTTGACGCAA CTGTGGCTCA GATTGAGCGC TGTGTTGATG CAGGTGCAGA TATTATGCGT GTTTCAGTGC CTTCTATGGA AGCTGCTGAA GCGTTTGGTG CAATCCGTAA GCGTGTTTCA GTTCCATTAG TTGCTGATAT CCATTTTGAC CATAGAATTG CTTTGGCAGT TGCAGATTAT GGTGCGGACT GCTTGCGTAT TAACCCGGGC AATATCGGTT CAGACCAGAA AGTTCGTGAA GTTGTGGCTG CGGCACGTCA TCACGGTATT TCTATGCGTA TTGGCGTGAA CGCGGGTTCT CTAGAAAAAG ATTTACAGAA AAAATATGGC GAGCCTACAG GGCAGGCACT TCTTGAGTCA GCTTTACGTC ATATTGATAT TTTAGACCGT CTTGACTTCC ATGAGTTTAA AGTCAGCGTA AAAGCATCAA ATGTGTTTTT AACTATGGAT GCTTATCGTT TACTTTCTCA ACAAATTGAT AATCCATTAC ACCTTGGAGT GACTGAAGCT GGTATTTACC GTACAGGTAC TGTGAAATCA GCGATTGCTC TTGGTGGGTT ATTAATGGAA GGCATTGGCG ATACGATGCG TATTTCGCTT GCTGCTGAAC CTGAAGATGA AATCAAGATC GGTTTTGATA TCTTAAAATC GCTTGGCCTA CGTTCTAACG GTATTAACTT TATTGCTTGT CCAAGTTGTT CTCGCCAAGA ATTTAACGTG ATTCAGGTGA TGCAGGCTTT AGAAGAGCGT TTAGAAGATA TCCGTACACC AATGGACGTT TCGGTTATTG GCTGTAAGGT AAATGGCCCA GGTGAAGCAA AAGAAGCAGA TATTGGGGTC GTTGGGGCTG CGCCTCGTTC ATTGGTTTAT CGTAATGGTG AGAAAAGCCA TTTAATTGAT ACCAATCAAT TGGTTGATGA AATCGAAACT ATGGTTCGTC AACGTGTTCA AGAGCTTGAA GAAGCTAAAT CTAAAGAAAT TATTCGTAGT TCATCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T