AcbaC.17085.a
coproporphyrinogen III oxidase, aerobic
| CENTER ID: | AcbaC.17085.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17085.a.B1.GE38743 | full length | 1 | 316 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.17085.a.B1.PS38131 | full length | 1 | 316 |
Structures
5EO6
DEPOSITED: 11/10/2015
DETERMINATION: XRay
CLONE: AcbaC.17085.a.B1.GE38743
PROTEIN: AcbaC.17085.a.B1.PS02404
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30163.1 |
| PATRIC ID: | fig|470.1345.peg.363 |
| PGAT: | acinetobacter:ABUW_0377 |
| RefSeq: | WP_001164614.1 |
| UniProt: | A0A0D5YE19 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQHPTSTDIQ RVREFLLDLQ ARICAGLEQQ EKAGGGTAEF IIDDWERPEG GGGRSRVLQN GTVIEKGGVM FSHINISKLP ASATERHPQI AGAKAQALGV SLVIHPKNPN IPTSHANVRL FVAEREDQDP IWWFGGGFDL TPFYPDDQDV LNWHQAAYDL CKPFGDNVYA EHKKWCDDYF YLKHRDEQRG VGGLFFDDLN CWDFETCFKY IQAVGNGYLN AILPIFEKHR EQPYTEAQRE FQLYRRGRYV EYNLVYDRGT LFGLQTGGRI ESILVSLPNL AAWSYRPEWD EDSPEKRLTD YYLKPRDWLG LEEKVA
NT Sequence
ATGCAACATC CGACTTCAAC AGATATTCAA CGTGTCCGCG AATTTCTCCT CGATTTACAA GCCCGTATTT GTGCCGGTTT AGAACAACAA GAAAAAGCTG GTGGTGGGAC AGCAGAATTT ATCATTGATG ATTGGGAGCG CCCTGAAGGC GGTGGCGGCC GTTCACGTGT ACTGCAAAAT GGTACAGTGA TTGAAAAAGG CGGCGTGATG TTTTCACATA TCAACATTAG TAAACTGCCT GCTTCTGCAA CAGAACGTCA CCCTCAAATT GCAGGGGCAA AAGCTCAAGC GCTAGGTGTG TCTTTAGTCA TTCACCCTAA AAACCCGAAT ATTCCAACCT CGCATGCGAA TGTCCGCTTA TTCGTTGCAG AGCGTGAAGA TCAAGATCCG ATTTGGTGGT TTGGTGGAGG TTTTGACTTA ACGCCATTTT ATCCAGATGA CCAAGATGTT TTAAATTGGC ACCAAGCCGC TTATGACCTC TGTAAGCCTT TTGGTGACAA TGTTTATGCT GAACATAAAA AATGGTGCGA TGACTATTTC TATTTAAAGC ATCGCGATGA ACAACGTGGT GTGGGCGGTT TATTCTTTGA TGATTTAAAT TGCTGGGATT TCGAAACCTG CTTTAAATAT ATTCAAGCCG TAGGTAATGG CTATTTAAAT GCGATCTTGC CAATTTTCGA AAAACACCGC GAACAGCCTT ATACCGAAGC TCAACGCGAA TTTCAGCTTT ATCGCCGTGG CAGATATGTC GAATATAACT TGGTGTATGA CCGCGGTACG TTGTTTGGAT TACAAACAGG TGGCCGTATT GAGTCGATTT TAGTTAGCTT ACCTAATCTA GCTGCATGGT CTTATCGCCC TGAATGGGAT GAAGACTCTC CAGAGAAGCG TTTAACTGAC TACTATTTAA AACCTCGTGA TTGGTTAGGT TTAGAAGAAA AAGTCGCCTA A
Details for AcbaC.17085.a.B1.GE38743
| HARVESTED ON: | 1/22/2015 |
| SEQUENCED ON: | 1/15/2015 |
| EXPECTED MW: | 37kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMQ HPTSTDIQRV REFLLDLQAR ICAGLEQQEK AGGGTAEFII DDWERPEGGG GRSRVLQNGT VIEKGGVMFS HINISKLPAS ATERHPQIAG AKAQALGVSL VIHPKNPNIP TSHANVRLFV AEREDQDPIW WFGGGFDLTP FYPDDQDVLN WHQAAYDLCK PFGDNVYAEH KKWCDDYFYL KHRDEQRGVG GLFFDDLNCW DFETCFKYIQ AVGNGYLNAI LPIFEKHREQ PYTEAQREFQ LYRRGRYVEY NLVYDRGTLF GLQTGGRIES ILVSLPNLAA WSYRPEWDED SPEKRLTDYY LKPRDWLGLE EKVA
Validated NT Sequence
tnnnttaggc gactttttct tctaaaccta accaatcacg aggttttaaa tagtagtcag ttaaacgctt ctctggagag tcttcatccc attcagggcg ataagaccat gcagctagat taggtaagct aactaaaatc gactcaatac ggccacctgt ttgtaatcca aacaacgtac cgcggtcata caccaagtta tattcgacat atctgccacg gcgataaagc tgaaattcgc gttgagcttc ggtataaggc tgttcgcggt gtttttcgaa aattggcaag atcgcattta aatagccatt acctacggct tgaatatatt taaagcaggt ttcgaaatcc cagcaattta aatcatcaaa gaataaaccg cccacaccac gttgttcatc gcgatgcttt aaatagaaat agtcatcgca ccatttttta tgttcagcat aaacattgtc accaaaaggc ttacagaggt cataagcggc ttggtgccaa tttaaaacat cttggtcatc tggataaaat ggcgttaagt caaaacctcc accaaaccac caaatcggat cttgatcttc acgctctgca acgaataagc ggacattcgc atgcgaggtt ggaatattcg ggtttttagg gtgaatgact aaagacacac ctagcgcttg agcttttgcc cctgcaattt gagggtgacg ttctgttgca gaagcaggca gtttactaat gttgatatgt gaaaacatca cgccgccttt ttcaatcact gtaccatttt gcagtacacg tgaacggccg ccaccgcctt cagggcgctc ccaatcatca atgataaatt ctgctgtccc accaccagct ttttcttgtt gttctaaacc ggcacaaata cgggcttgta aatcgaggag aaattcgcgg acacgttgaa tatctgttga agtcggatgt tgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMQ HPTSTDIQRV REFLLDLQAR ICAGLEQQEK AGGGTAEFII DDWERPEGGG GRSRVLQNGT VIEKGGVMFS HINISKLPAS ATERHPQIAG AKAQALGVSL VIHPKNPNIP TSHANVRLFV AEREDQDPIW WFGGGFDLTP FYPDDQDVLN WHQAAYDLCK PFGDNVYAEH KKWCDDYFYL KHRDEQRGVG GLFFDDLNCW DFETCFKYIQ AVGNGYLNAI LPIFEKHREQ PYTEAQREFQ LYRRGRYVEY NLVYDRGTLF GLQTGGRIES ILVSLPNLAA WSYRPEWDED SPEKRLTDYY LKPRDWLGLE EKVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac atccgacttc aacagatatt caacgtgtcc gcgaatttct cctcgattta caagcccgta tttgtgccgg tttagaacaa caagaaaaag ctggtggtgg gacagcagaa tttatcattg atgattggga gcgccctgaa ggcggtggcg gccgttcacg tgtactgcaa aatggtacag tgattgaaaa aggcggcgtg atgttttcac atatcaacat tagtaaactg cctgcttctg caacagaacg tcaccctcaa attgcagggg caaaagctca agcgctaggt gtgtctttag tcattcaccc taaaaacccg aatattccaa cctcgcatgc gaatgtccgc ttattcgttg cagagcgtga agatcaagat ccgatttggt ggtttggtgg aggttttgac ttaacgccat tttatccaga tgaccaagat gttttaaatt ggcaccaagc cgcttatgac ctctgtaagc cttttggtga caatgtttat gctgaacata aaaaatggtg cgatgactat ttctatttaa agcatcgcga tgaacaacgt ggtgtgggcg gtttattctt tgatgattta aattgctggg atttcgaaac ctgctttaaa tatattcaag ccgtaggtaa tggctattta aatgcgatct tgccaatttt cgaaaaacac cgcgaacagc cttataccga agctcaacgc gaatttcagc tttatcgccg tggcagatat gtcgaatata acttggtgta tgaccgcggt acgttgtttg gattacaaac aggtggccgt attgagtcga ttttagttag cttacctaat ctagctgcat ggtcttatcg ccctgaatgg gatgaagact ctccagagaa gcgtttaact gactactatt taaaacctcg tgattggtta ggtttagaag aaaaagtcgc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for AcbaC.17085.a.B1.PS38131
| PURIFICATION DATe: | 11/29/2016 |
| CONCENTRATION: | 44.21mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ HPTSTDIQRV REFLLDLQAR ICAGLEQQEK AGGGTAEFII DDWERPEGGG GRSRVLQNGT VIEKGGVMFS HINISKLPAS ATERHPQIAG AKAQALGVSL VIHPKNPNIP TSHANVRLFV AEREDQDPIW WFGGGFDLTP FYPDDQDVLN WHQAAYDLCK PFGDNVYAEH KKWCDDYFYL KHRDEQRGVG GLFFDDLNCW DFETCFKYIQ AVGNGYLNAI LPIFEKHREQ PYTEAQREFQ LYRRGRYVEY NLVYDRGTLF GLQTGGRIES ILVSLPNLAA WSYRPEWDED SPEKRLTDYY LKPRDWLGLE EKVA
Validated NT Sequence
tnnnttaggc gactttttct tctaaaccta accaatcacg aggttttaaa tagtagtcag ttaaacgctt ctctggagag tcttcatccc attcagggcg ataagaccat gcagctagat taggtaagct aactaaaatc gactcaatac ggccacctgt ttgtaatcca aacaacgtac cgcggtcata caccaagtta tattcgacat atctgccacg gcgataaagc tgaaattcgc gttgagcttc ggtataaggc tgttcgcggt gtttttcgaa aattggcaag atcgcattta aatagccatt acctacggct tgaatatatt taaagcaggt ttcgaaatcc cagcaattta aatcatcaaa gaataaaccg cccacaccac gttgttcatc gcgatgcttt aaatagaaat agtcatcgca ccatttttta tgttcagcat aaacattgtc accaaaaggc ttacagaggt cataagcggc ttggtgccaa tttaaaacat cttggtcatc tggataaaat ggcgttaagt caaaacctcc accaaaccac caaatcggat cttgatcttc acgctctgca acgaataagc ggacattcgc atgcgaggtt ggaatattcg ggtttttagg gtgaatgact aaagacacac ctagcgcttg agcttttgcc cctgcaattt gagggtgacg ttctgttgca gaagcaggca gtttactaat gttgatatgt gaaaacatca cgccgccttt ttcaatcact gtaccatttt gcagtacacg tgaacggccg ccaccgcctt cagggcgctc ccaatcatca atgataaatt ctgctgtccc accaccagct ttttcttgtt gttctaaacc ggcacaaata cgggcttgta aatcgaggag aaattcgcgg acacgttgaa tatctgttga agtcggatgt tgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMQ HPTSTDIQRV REFLLDLQAR ICAGLEQQEK AGGGTAEFII DDWERPEGGG GRSRVLQNGT VIEKGGVMFS HINISKLPAS ATERHPQIAG AKAQALGVSL VIHPKNPNIP TSHANVRLFV AEREDQDPIW WFGGGFDLTP FYPDDQDVLN WHQAAYDLCK PFGDNVYAEH KKWCDDYFYL KHRDEQRGVG GLFFDDLNCW DFETCFKYIQ AVGNGYLNAI LPIFEKHREQ PYTEAQREFQ LYRRGRYVEY NLVYDRGTLF GLQTGGRIES ILVSLPNLAA WSYRPEWDED SPEKRLTDYY LKPRDWLGLE EKVA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC ATCCGACTTC AACAGATATT CAACGTGTCC GCGAATTTCT CCTCGATTTA CAAGCCCGTA TTTGTGCCGG TTTAGAACAA CAAGAAAAAG CTGGTGGTGG GACAGCAGAA TTTATCATTG ATGATTGGGA GCGCCCTGAA GGCGGTGGCG GCCGTTCACG TGTACTGCAA AATGGTACAG TGATTGAAAA AGGCGGCGTG ATGTTTTCAC ATATCAACAT TAGTAAACTG CCTGCTTCTG CAACAGAACG TCACCCTCAA ATTGCAGGGG CAAAAGCTCA AGCGCTAGGT GTGTCTTTAG TCATTCACCC TAAAAACCCG AATATTCCAA CCTCGCATGC GAATGTCCGC TTATTCGTTG CAGAGCGTGA AGATCAAGAT CCGATTTGGT GGTTTGGTGG AGGTTTTGAC TTAACGCCAT TTTATCCAGA TGACCAAGAT GTTTTAAATT GGCACCAAGC CGCTTATGAC CTCTGTAAGC CTTTTGGTGA CAATGTTTAT GCTGAACATA AAAAATGGTG CGATGACTAT TTCTATTTAA AGCATCGCGA TGAACAACGT GGTGTGGGCG GTTTATTCTT TGATGATTTA AATTGCTGGG ATTTCGAAAC CTGCTTTAAA TATATTCAAG CCGTAGGTAA TGGCTATTTA AATGCGATCT TGCCAATTTT CGAAAAACAC CGCGAACAGC CTTATACCGA AGCTCAACGC GAATTTCAGC TTTATCGCCG TGGCAGATAT GTCGAATATA ACTTGGTGTA TGACCGCGGT ACGTTGTTTG GATTACAAAC AGGTGGCCGT ATTGAGTCGA TTTTAGTTAG CTTACCTAAT CTAGCTGCAT GGTCTTATCG CCCTGAATGG GATGAAGACT CTCCAGAGAA GCGTTTAACT GACTACTATT TAAAACCTCG TGATTGGTTA GGTTTAGAAG AAAAAGTCGC CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT