SSGCID: Target Page

AcbaC.10014.a

lipoprotein releasing system, ATP-binding protein

CENTER ID:	AcbaC.10014.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.10014.a.B1.GE38741	full length		1	228

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.940
GenBank:	AKA30729.1
PGAT:	acinetobacter:ABUW_0975
RefSeq:	WP_000049406.1
UniProt:	V5VC90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKVVLEAKD IYKHFDDGKS KVEVIKGLSL QVEAGQFVSI VGASGSGKST LLHVLGGLDQ PTKGQVFLNG QRFDNLGEAE RGFQRNQYLG FVYQFHHLLP EFTALENVAM PLMLRADSQY KSVKAQAEYL LDRVGLSHRM DHKPGELSGG ERQRVALARA LVTKPAVVLA DEPTGNLDRK TAVGIFELLT DLKKELNMAM LIVTHDEQLA QAADSILHME DGLWVNGS

NT Sequence

ATGAGTAAAG TCGTTTTAGA AGCAAAAGAT ATTTATAAAC ATTTTGATGA CGGTAAATCT AAGGTTGAAG TCATTAAAGG TCTCTCTTTA CAAGTGGAAG CAGGGCAGTT TGTTTCGATT GTCGGGGCAA GTGGTTCAGG TAAAAGTACT TTGTTGCATG TACTTGGTGG CTTAGATCAG CCGACTAAAG GACAGGTATT TTTGAATGGT CAGCGCTTTG ATAATTTAGG TGAAGCAGAA CGTGGCTTTC AACGTAACCA ATACTTAGGT TTTGTTTACC AGTTCCACCA TTTATTACCT GAGTTTACCG CTCTTGAAAA TGTAGCGATG CCTTTAATGC TTCGAGCAGA CAGTCAATAT AAGTCGGTAA AGGCACAGGC TGAATATTTG CTAGATCGAG TTGGGCTATC TCATCGTATG GACCATAAAC CAGGTGAACT TTCAGGTGGT GAGCGTCAAC GTGTTGCGCT TGCTCGTGCA TTAGTGACTA AGCCTGCGGT AGTACTAGCT GATGAGCCTA CGGGTAACCT AGATCGTAAA ACAGCAGTAG GTATTTTCGA GTTGCTCACC GATCTTAAGA AAGAACTAAA CATGGCAATG TTAATTGTGA CCCATGACGA GCAATTAGCT CAGGCAGCAG ATTCAATCTT GCATATGGAA GATGGGCTGT GGGTAAACGG TTCTTAA

Details for AcbaC.10014.a.B1.GE38741

HARVESTED ON:	data unavailable
SEQUENCED ON:	1/15/2015
EXPECTED MW:	26kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS KVVLEAKDIY KHFDDGKSKV EVIKGLSLQV EAGQFVSIVG ASGSGKSTLL HVLGGLDQPT KGQVFLNGQR FDNLGEAERG FQRNQYLGFV YQFHHLLPEF TALENVAMPL MLRADSQYKS VKAQAEYLLD RVGLSHRMDH KPGELSGGER QRVALARALV TKPAVVLADE PTGNLDRKTA VGIFELLTDL KKELNMAMLI VTHDEQLAQA ADSILHMEDG LWVNGS

Validated NT Sequence

atcttactca cttaagaacc gtttacccac agcccatctt ccatatgcaa gattgaatct gctgcctgag ctaattgctc gtcatgggtc acaattaaca ttgccatgtt tagttctttc ttaagatcgg tgagcaactc gaaaatacct actgctgttt tacgatctag gttacccgta ggctcatcag ctagtactac cgcaggctta gtcactaatg cacgagcaag cgcaacacgt tgacgctcac cacctgaaag ttcacctggt ttatggtcca tacgatgaga tagcccaact cgatctagca aatattcagc ctgtgccttt accgacttat attgactgtc tgctcgaagc attaaaggca tcgctacatt ttcaagagcg gtaaactcag gtaataaatg gtggaactgg taaacaaaac ctaagtattg gttacgttga aagccacgtt ctgcttcacc taaattatca aagcgctgac cattcaaaaa tacctgtcct ttagtcggct gatctaagcc accaagtaca tgcaacaaag tacttttacc tgaaccactt gccccgacaa tcgaaacaaa ctgccctgct tccacttgta aagagagacc tttaatgact tcaaccttag atttaccgtc atcaaaatgt ttataaatat cttttgcttc taaaacgact ttactcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta aagtcgtttt agaagcaaaa gatatttata aacattttga tgacggtaaa tctaaggttg aagtcattaa aggtctctct ttacaagtgg aagcagggca gtttgtttcg attgtcgggg caagtggttc aggtaaaagt actttgttgc atgtacttgg tggcttagat cagccgacta aaggacaggt atttttgaat ggtcagcgct ttgataattt aggtgaagca gaacgtggct ttcaacgtaa ccaatactta ggttttgttt accagttcca ccatttatta cctgagttta ccgctcttga aaatgtagcg atgcctttaa tgcttcgagc agacagtcaa tataagtcgg taaaggcaca ggctgaatat ttgctagatc gagttgggct atctcatcgt atggaccata aaccaggtga actttcaggt ggtgagcgtc aacgtgttgc gcttgctcgt gcattagtga ctaagcctgc ggtagtacta gctgatgagc ctacgggtaa cctagatcgt aaaacagcag taggtatttt cgagttgctc accgatctta agaaagaact aaacatggca atgttaattg tgacccatga cgagcaatta gctcaggcag cagattcaat cttgcatatg gaagatgggc tgtgggtaaa cggttcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at