AcbaC.01463.a

guanylate kinase

CENTER ID: AcbaC.01463.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01463.a.B2.GE38691 mature protein 23 209
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.298
GenBank: AKA30097.1
PGAT: acinetobacter:ABUW_0311
RefSeq: WP_000015937.1
UniProt: A0A059ZQZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGLLFVVSA ASGTGKTSLV KALLDRVSNL HVSVSHTTRG QRPGELDGVH YHFTTKDEFL DQVNQGGFIE YAEVFGNYYG TSQATVKQQL AQGHDVLLEI DWQGAEQVRK LFPESKQIFI LPPTQFDLRQ RLSNRGTDSV EVIEHRLSCA VEDMQHYVNF DYIIINDDFN KALHELEAVI TANRLVLSQQ AKRHQNLIQD LITPQPKQE
NT Sequence
ATGTCGGGTC TATTGTTTGT CGTTTCTGCT GCATCAGGAA CGGGCAAAAC ATCTTTGGTG AAAGCCCTAC TTGACCGTGT GAGCAATTTA CATGTTTCAG TTTCGCATAC TACCCGAGGT CAACGACCAG GTGAGTTAGA TGGTGTTCAC TATCACTTCA CAACAAAAGA TGAATTTTTA GACCAAGTGA ACCAAGGTGG ATTTATCGAA TATGCTGAAG TATTTGGTAA TTACTATGGC ACTTCGCAAG CAACCGTAAA ACAACAGTTA GCTCAAGGAC ATGATGTCTT GCTTGAAATT GACTGGCAAG GTGCAGAGCA AGTACGCAAG CTGTTTCCTG AATCTAAACA GATCTTTATT TTACCACCTA CTCAATTCGA TTTACGTCAG CGTTTATCTA ACCGTGGTAC AGACTCTGTT GAAGTTATTG AACACCGCTT AAGCTGTGCT GTTGAAGACA TGCAGCATTA TGTAAATTTC GACTATATCA TCATTAATGA TGACTTTAAT AAAGCCCTAC ATGAGCTTGA AGCCGTCATT ACAGCAAATC GTTTAGTCTT ATCTCAACAG GCAAAAAGAC ATCAAAATCT AATTCAAGAC TTGATTACTC CACAACCAAA ACAGGAATAA
Details for AcbaC.01463.a.B2.GE38691
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML LDRVSNLHVS VSHTTRGQRP GELDGVHYHF TTKDEFLDQV NQGGFIEYAE VFGNYYGTSQ ATVKQQLAQG HDVLLEIDWQ GAEQVRKLFP ESKQIFILPP TQFDLRQRLS NRGTDSVEVI EHRLSCAVED MQHYVNFDYI IINDDFNKAL HELEAVITAN RLVLSQQAKR HQNLIQDLIT PQPKQE
Validated NT Sequence
atcttactca cttattcctg ttttggttgt ggagtaatca agtcttgaat tagattttga tgtctttttg cctgttgaga taagactaaa cgatttgctg taatgacggc ttcaagctca tgtagggctt tattaaagtc atcattaatg atgatatagt cgaaatttac ataatgctgc atgtcttcaa cagcacagct taagcggtgt tcaataactt caacagagtc tgtaccacgg ttagataaac gctgacgtaa atcgaattga gtaggtggta aaataaagat ctgtttagat tcaggaaaca gcttgcgtac ttgctctgca ccttgccagt caatttcaag caagacatca tgtccttgag ctaactgttg ttttacggtt gcttgcgaag tgccatagta attaccaaat acttcagcat attcgataaa tccaccttgg ttcacttggt ctaaaaattc atcttttgtt gtgaagtgat agtgaacacc atctaactca cctggtcgtt gacctcgggt agtatgcgaa actgaaacat gtaaattgct cacacggtca agtagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHLL DRVSNLHVSV SHTTRGQRPG ELDGVHYHFT TKDEFLDQVN QGGFIEYAEV FGNYYGTSQA TVKQQLAQGH DVLLEIDWQG AEQVRKLFPE SKQIFILPPT QFDLRQRLSN RGTDSVEVIE HRLSCAVEDM QHYVNFDYII INDDFNKALH ELEAVITANR LVLSQQAKRH QNLIQDLITP QPKQE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctacttg accgtgtgag caatttacat gtttcagttt cgcatactac ccgaggtcaa cgaccaggtg agttagatgg tgttcactat cacttcacaa caaaagatga atttttagac caagtgaacc aaggtggatt tatcgaatat gctgaagtat ttggtaatta ctatggcact tcgcaagcaa ccgtaaaaca acagttagct caaggacatg atgtcttgct tgaaattgac tggcaaggtg cagagcaagt acgcaagctg tttcctgaat ctaaacagat ctttatttta ccacctactc aattcgattt acgtcagcgt ttatctaacc gtggtacaga ctctgttgaa gttattgaac accgcttaag ctgtgctgtt gaagacatgc agcattatgt aaatttcgac tatatcatca ttaatgatga ctttaataaa gccctacatg agcttgaagc cgtcattaca gcaaatcgtt tagtcttatc tcaacaggca aaaagacatc aaaatctaat tcaagacttg attactccac aaccaaaaca ggaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat