AcbaC.01416.a
fructose-bisphosphate aldolase, class II, Calvin cycle subtype
| CENTER ID: | AcbaC.01416.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01416.a.B1.GE38690 | full length | 1 | 345 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01416.a.B1.PS02365 | full length | 1 | 345 |
Structures
5U7S
DEPOSITED: 12/12/2016
DETERMINATION: XRay
CLONE: AcbaC.01416.a.B1.GE38690
PROTEIN: AcbaC.01416.a.B1.PS02365
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA31997.1 |
| PATRIC ID: | fig|470.1345.peg.2224 |
| PGAT: | acinetobacter:ABUW_2269 |
| RefSeq: | WP_001174011.1 |
| UniProt: | V5VDS0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALISMRQLL DHAAEHNYGV PAFNVNNLEQ MRAIMLAADA TNSPVIVQAS AGARKYAGAP FLRHLILAAI EEWPHIPVVM HQDHGTSPDV CQRSIQLGFS SVMMDGSLGA DGKTPTTYDY NVDVTRQVVA MAHACGVSVE GEIGCLGSLE TGMAGEEDGV GAEGVLDHSQ LLTSVEEAKQ FVADTNVDAL AIAVGTSHGA YKFTRPPTGD ILAIDRIKEI HAALPNTHLV MHGSSSVPQE WLKVINEFGG NIGETYGVPV EQLVEAIKHG VRKINIDTDL RLASTGAIRR FMAENPAEFD PRKYFAKTVD SMKQICIDRY EAFGTAGNAD KIRPISLEKM VDRYK
NT Sequence
ATGGCACTCA TTTCAATGCG CCAGCTCTTG GATCACGCTG CGGAACATAA CTACGGCGTA CCAGCATTTA ACGTAAATAA CTTAGAACAA ATGCGTGCAA TTATGCTTGC CGCAGATGCT ACCAATTCAC CTGTAATTGT TCAGGCATCT GCTGGAGCTC GCAAATATGC AGGCGCTCCA TTCTTACGCC ATCTTATTTT GGCTGCAATT GAAGAATGGC CACACATTCC AGTGGTAATG CACCAAGACC ATGGTACAAG CCCTGATGTA TGCCAACGTT CTATTCAACT TGGTTTTAGC TCAGTGATGA TGGACGGTTC ATTAGGCGCT GATGGTAAAA CTCCTACAAC TTACGATTAC AACGTAGATG TAACTCGTCA AGTGGTAGCA ATGGCTCATG CTTGTGGTGT ATCTGTTGAA GGTGAGATCG GTTGCTTAGG TAGCCTTGAA ACAGGTATGG CTGGAGAAGA AGACGGTGTG GGTGCAGAAG GTGTACTTGA CCATTCTCAG CTTTTAACCT CTGTTGAAGA AGCAAAACAA TTCGTTGCTG ATACAAATGT TGATGCTTTA GCAATTGCTG TAGGTACTTC ACACGGTGCT TATAAATTTA CACGTCCACC TACAGGGGAT ATTTTAGCAA TTGACCGTAT TAAAGAAATT CATGCGGCAT TGCCAAATAC TCACCTTGTA ATGCACGGTT CAAGCTCTGT ACCACAAGAA TGGTTAAAAG TAATTAACGA GTTTGGTGGT AATATTGGTG AGACTTATGG TGTACCAGTT GAACAGCTTG TAGAAGCGAT TAAACATGGT GTACGTAAAA TCAATATCGA TACTGACTTG CGTTTAGCTT CAACAGGTGC AATTCGTCGT TTCATGGCGG AAAACCCAGC TGAATTTGAC CCACGTAAGT ATTTCGCTAA AACTGTCGAT TCAATGAAAC AAATTTGTAT CGATCGTTAT GAAGCTTTCG GTACAGCTGG TAATGCTGAC AAGATTCGTC CAATTTCTTT AGAGAAAATG GTTGATCGTT ATAAATAA
Details for AcbaC.01416.a.B1.GE38690
| HARVESTED ON: | 12/9/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMA LISMRQLLDH AAEHNYGVPA FNVNNLEQMR AIMLAADATN SPVIVQASAG ARKYAGAPFL RHLILAAIEE WPHIPVVMHQ DHGTSPDVCQ RSIQLGFSSV MMDGSLGADG KTPTTYDYNV DVTRQVVAMA HACGVSVEGE IGCLGSLETG MAGEEDGVGA EGVLDHSQLL TSVEEAKQFV ADTNVDALAI AVGTSHGAYK FTRPPTGDIL AIDRIKEIHA ALPNTHLVMH GSSSVPQEWL KVINEFGGNI GETYGVPVEQ LVEAIKHGVR KINIDTDLRL ASTGAIRRFM AENPAEFDPR KYFAKTVDSM KQICIDRYEA FGTAGNADKI RPISLE
Validated NT Sequence
tctctaaaga aattggacga atcttgtcag cattaccagc tgtaccgaaa gcttcataac gatcgataca aatttgtttc attgaatcga cagttttagc gaaatactta cgtgggtcaa attcagctgg gttttccgcc atgaaacgac gaattgcacc tgttgaagct aaacgcaagt cagtatcgat attgatttta cgtacaccat gtttaatcgc ttctacaagc tgttcaactg gtacaccata agtctcacca atattaccac caaactcgtt aattactttt aaccattctt gtggtacaga gcttgaaccg tgcattacaa ggtgagtatt tggcaatgcc gcatgaattt ctttaatacg gtcaattgct aaaatatccc ctgtaggtgg acgtgtaaat ttataagcac cgtgtgaagt acctacagca attgctaaag catcaacatt tgtatcagca acgaattgtt ttgcttcttc aacagaggtt aaaagctgag aatggtcaag tacaccttct gcacccacac cgtcttcttc tccagccata cctgtttcaa ggctacctaa gcaaccgatc tcaccttcaa cagatacacc acaagcatga gccattgcta ccacttgacg agttacatct acgttgtaat cgtaagttgt aggagtttta ccatcagcgc ctaatgaacc gtccatcatc actgagctaa aaccaagttg aatagaacgt tggcatacat cagggcttgt accatggtct tggtgcatta ccactggaat gtgtggccat tcttcaattg cagccaaaat aagatggcgt aagaatggag cgcctgcata tttgcgagct ccagcagatg cctgaacaat tacaggtgaa ttggtagcat ctgcggcaag cataattgca cgcatttgtt ctaagttatt tacgttaaat gctggtacgc cgtagttatg ttccgcagcg tgatccaaga gctggcgcat tgaaatgagt gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA LISMRQLLDH AAEHNYGVPA FNVNNLEQMR AIMLAADATN SPVIVQASAG ARKYAGAPFL RHLILAAIEE WPHIPVVMHQ DHGTSPDVCQ RSIQLGFSSV MMDGSLGADG KTPTTYDYNV DVTRQVVAMA HACGVSVEGE IGCLGSLETG MAGEEDGVGA EGVLDHSQLL TSVEEAKQFV ADTNVDALAI AVGTSHGAYK FTRPPTGDIL AIDRIKEIHA ALPNTHLVMH GSSSVPQEWL KVINEFGGNI GETYGVPVEQ LVEAIKHGVR KINIDTDLRL ASTGAIRRFM AENPAEFDPR KYFAKTVDSM KQICIDRYEA FGTAGNADKI RPISLEKMVD RYK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac tcatttcaat gcgccagctc ttggatcacg ctgcggaaca taactacggc gtaccagcat ttaacgtaaa taacttagaa caaatgcgtg caattatgct tgccgcagat gctaccaatt cacctgtaat tgttcaggca tctgctggag ctcgcaaata tgcaggcgct ccattcttac gccatcttat tttggctgca attgaagaat ggccacacat tccagtggta atgcaccaag accatggtac aagccctgat gtatgccaac gttctattca acttggtttt agctcagtga tgatggacgg ttcattaggc gctgatggta aaactcctac aacttacgat tacaacgtag atgtaactcg tcaagtggta gcaatggctc atgcttgtgg tgtatctgtt gaaggtgaga tcggttgctt aggtagcctt gaaacaggta tggctggaga agaagacggt gtgggtgcag aaggtgtact tgaccattct cagcttttaa cctctgttga agaagcaaaa caattcgttg ctgatacaaa tgttgatgct ttagcaattg ctgtaggtac ttcacacggt gcttataaat ttacacgtcc acctacaggg gatattttag caattgaccg tattaaagaa attcatgcgg cattgccaaa tactcacctt gtaatgcacg gttcaagctc tgtaccacaa gaatggttaa aagtaattaa cgagtttggt ggtaatattg gtgagactta tggtgtacca gttgaacagc ttgtagaagc gattaaacat ggtgtacgta aaatcaatat cgatactgac ttgcgtttag cttcaacagg tgcaattcgt cgtttcatgg cggaaaaccc agctgaattt gacccacgta agtatttcgc taaaactgtc gattcaatga aacaaatttg tatcgatcgt tatgaagctt tcggtacagc tggtaatgct gacaagattc gtccaatttc tttagagaaa atggttgatc gttataaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for AcbaC.01416.a.B1.PS02365
| PURIFICATION DATe: | 3/5/2015 |
| CONCENTRATION: | 36mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA LISMRQLLDH AAEHNYGVPA FNVNNLEQMR AIMLAADATN SPVIVQASAG ARKYAGAPFL RHLILAAIEE WPHIPVVMHQ DHGTSPDVCQ RSIQLGFSSV MMDGSLGADG KTPTTYDYNV DVTRQVVAMA HACGVSVEGE IGCLGSLETG MAGEEDGVGA EGVLDHSQLL TSVEEAKQFV ADTNVDALAI AVGTSHGAYK FTRPPTGDIL AIDRIKEIHA ALPNTHLVMH GSSSVPQEWL KVINEFGGNI GETYGVPVEQ LVEAIKHGVR KINIDTDLRL ASTGAIRRFM AENPAEFDPR KYFAKTVDSM KQICIDRYEA FGTAGNADKI RPISLE
Validated NT Sequence
tctctaaaga aattggacga atcttgtcag cattaccagc tgtaccgaaa gcttcataac gatcgataca aatttgtttc attgaatcga cagttttagc gaaatactta cgtgggtcaa attcagctgg gttttccgcc atgaaacgac gaattgcacc tgttgaagct aaacgcaagt cagtatcgat attgatttta cgtacaccat gtttaatcgc ttctacaagc tgttcaactg gtacaccata agtctcacca atattaccac caaactcgtt aattactttt aaccattctt gtggtacaga gcttgaaccg tgcattacaa ggtgagtatt tggcaatgcc gcatgaattt ctttaatacg gtcaattgct aaaatatccc ctgtaggtgg acgtgtaaat ttataagcac cgtgtgaagt acctacagca attgctaaag catcaacatt tgtatcagca acgaattgtt ttgcttcttc aacagaggtt aaaagctgag aatggtcaag tacaccttct gcacccacac cgtcttcttc tccagccata cctgtttcaa ggctacctaa gcaaccgatc tcaccttcaa cagatacacc acaagcatga gccattgcta ccacttgacg agttacatct acgttgtaat cgtaagttgt aggagtttta ccatcagcgc ctaatgaacc gtccatcatc actgagctaa aaccaagttg aatagaacgt tggcatacat cagggcttgt accatggtct tggtgcatta ccactggaat gtgtggccat tcttcaattg cagccaaaat aagatggcgt aagaatggag cgcctgcata tttgcgagct ccagcagatg cctgaacaat tacaggtgaa ttggtagcat ctgcggcaag cataattgca cgcatttgtt ctaagttatt tacgttaaat gctggtacgc cgtagttatg ttccgcagcg tgatccaaga gctggcgcat tgaaatgagt gccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMA LISMRQLLDH AAEHNYGVPA FNVNNLEQMR AIMLAADATN SPVIVQASAG ARKYAGAPFL RHLILAAIEE WPHIPVVMHQ DHGTSPDVCQ RSIQLGFSSV MMDGSLGADG KTPTTYDYNV DVTRQVVAMA HACGVSVEGE IGCLGSLETG MAGEEDGVGA EGVLDHSQLL TSVEEAKQFV ADTNVDALAI AVGTSHGAYK FTRPPTGDIL AIDRIKEIHA ALPNTHLVMH GSSSVPQEWL KVINEFGGNI GETYGVPVEQ LVEAIKHGVR KINIDTDLRL ASTGAIRRFM AENPAEFDPR KYFAKTVDSM KQICIDRYEA FGTAGNADKI RPISLEKMVD RYK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC TCATTTCAAT GCGCCAGCTC TTGGATCACG CTGCGGAACA TAACTACGGC GTACCAGCAT TTAACGTAAA TAACTTAGAA CAAATGCGTG CAATTATGCT TGCCGCAGAT GCTACCAATT CACCTGTAAT TGTTCAGGCA TCTGCTGGAG CTCGCAAATA TGCAGGCGCT CCATTCTTAC GCCATCTTAT TTTGGCTGCA ATTGAAGAAT GGCCACACAT TCCAGTGGTA ATGCACCAAG ACCATGGTAC AAGCCCTGAT GTATGCCAAC GTTCTATTCA ACTTGGTTTT AGCTCAGTGA TGATGGACGG TTCATTAGGC GCTGATGGTA AAACTCCTAC AACTTACGAT TACAACGTAG ATGTAACTCG TCAAGTGGTA GCAATGGCTC ATGCTTGTGG TGTATCTGTT GAAGGTGAGA TCGGTTGCTT AGGTAGCCTT GAAACAGGTA TGGCTGGAGA AGAAGACGGT GTGGGTGCAG AAGGTGTACT TGACCATTCT CAGCTTTTAA CCTCTGTTGA AGAAGCAAAA CAATTCGTTG CTGATACAAA TGTTGATGCT TTAGCAATTG CTGTAGGTAC TTCACACGGT GCTTATAAAT TTACACGTCC ACCTACAGGG GATATTTTAG CAATTGACCG TATTAAAGAA ATTCATGCGG CATTGCCAAA TACTCACCTT GTAATGCACG GTTCAAGCTC TGTACCACAA GAATGGTTAA AAGTAATTAA CGAGTTTGGT GGTAATATTG GTGAGACTTA TGGTGTACCA GTTGAACAGC TTGTAGAAGC GATTAAACAT GGTGTACGTA AAATCAATAT CGATACTGAC TTGCGTTTAG CTTCAACAGG TGCAATTCGT CGTTTCATGG CGGAAAACCC AGCTGAATTT GACCCACGTA AGTATTTCGC TAAAACTGTC GATTCAATGA AACAAATTTG TATCGATCGT TATGAAGCTT TCGGTACAGC TGGTAATGCT GACAAGATTC GTCCAATTTC TTTAGAGAAA ATGGTTGATC GTTATAAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT