AcbaC.01370.a

phosphoglucosamine mutase

CENTER ID: AcbaC.01370.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01370.a.B1.GE38689 full length 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.159
GenBank: AKA29958.1
PGAT: acinetobacter:ABUW_0163
RefSeq: WP_000119870.1
UniProt: A0A059ZEY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYFGTDGIR GKFGQMPITP EFALKLGFAA GKVLKRTSPK NKPLVVLGKD TRLSGYILES ALQAGLNAAG VYVHLLGPLP TPAIAHLTRA LHAHAGIVIS ASHNPYFDNG IKFFSSEGKK LPDSLQEEIN KELEKDLFIE DTANLGKSVR VTDANGRYIE FCKSTFPYHF DLNNLKIVVD CAHGAAYSVG PSVFRELGAK VVALYNEPDG LNINENCGST HPESLQKAVV EHGADLGIAF DGDADRVVMV DKFGNLIDGD HILYILATQA KNKPAGVVGT VMSNMALEVA LEKANVGFVR AKVGDRYVLQ ALEENGWVTG GEPSGHILTL DKSTTGDAII AALQVLTVMV EQNKALHELV NGFKLYPQVL VNVRLEQMLD PYSIPALVAE FNKAEEQLKG RGRILIRKSG TEPVIRVMVE GDNEQEVKTL AEHLANAVRS QAQVA
NT Sequence
ATGAGTTATT TTGGAACGGA TGGTATTCGT GGAAAATTTG GGCAAATGCC GATTACGCCA GAGTTTGCTT TAAAACTCGG TTTTGCTGCA GGGAAGGTAT TAAAACGAAC GAGTCCGAAA AATAAACCAC TTGTTGTATT AGGAAAAGAT ACTCGTTTAT CTGGTTATAT TTTAGAGTCT GCTTTGCAGG CGGGATTAAA TGCTGCTGGT GTATATGTTC ACTTACTTGG ACCATTACCT ACACCGGCTA TTGCACATCT TACCCGTGCC TTACATGCAC ATGCAGGGAT TGTTATTTCT GCATCACATA ACCCATATTT TGATAACGGG ATTAAGTTCT TCTCAAGTGA AGGTAAAAAA TTACCAGATT CATTGCAGGA AGAAATTAAT AAAGAATTAG AAAAAGATTT ATTTATCGAA GACACTGCAA ACTTAGGTAA AAGTGTTCGT GTGACCGATG CAAATGGTCG TTACATCGAA TTTTGTAAAT CTACATTCCC ATATCATTTT GATTTAAATA ATTTAAAAAT TGTTGTGGAT TGTGCTCACG GCGCTGCTTA CAGCGTTGGC CCTTCGGTTT TCCGTGAACT CGGCGCTAAA GTTGTCGCTT TATACAATGA GCCAGATGGT TTAAATATTA ATGAAAACTG TGGTTCAACT CATCCAGAGA GCTTACAAAA AGCGGTTGTG GAACATGGGG CTGACTTAGG TATTGCCTTT GATGGTGATG CTGACCGTGT AGTGATGGTT GATAAGTTCG GTAACTTAAT TGACGGTGAC CACATCTTAT ATATTTTAGC GACTCAGGCA AAAAATAAAC CAGCAGGTGT TGTTGGTACG GTAATGAGTA ACATGGCACT TGAAGTGGCA CTTGAAAAAG CAAATGTTGG CTTTGTTCGT GCAAAAGTCG GTGACCGTTA TGTATTACAA GCCCTAGAAG AAAATGGATG GGTAACAGGT GGTGAGCCTT CTGGTCATAT TCTGACTTTA GACAAGAGCA CAACAGGTGA TGCGATTATT GCAGCATTAC AAGTGTTAAC CGTGATGGTT GAGCAGAACA AAGCGCTGCA CGAATTGGTT AACGGCTTTA AACTGTACCC ACAAGTTCTT GTAAATGTAC GTTTGGAACA AATGCTTGAT CCATATTCTA TTCCTGCATT AGTTGCTGAG TTCAATAAAG CTGAAGAGCA GCTCAAAGGA CGTGGACGTA TTTTAATTCG TAAGTCTGGA ACTGAGCCAG TCATTCGTGT CATGGTTGAA GGTGATAACG AGCAAGAAGT GAAAACTTTA GCTGAACATT TAGCAAATGC AGTTCGTTCA CAGGCACAAG TCGCATAA
Details for AcbaC.01370.a.B1.GE38689
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS YFGTDGIRGK FGQMPITPEF ALKLGFAAGK VLKRTSPKNK PLVVLGKDTR LSGYILESAL QAGLNAAGVY VHLLGPLPTP AIAHLTRALH AHAGIVISAS HNPYFDNGIK FFSSEGKKLP DSLQEEINKE LEKDLFIEDT ANLGKSVRVT DANGRYIEFC KSTFPYHFDL NNLKIVVDCA HGAAYSVGPS VFRELGAKVV ALYNEPDGLN INENCGSTHP ESLQKAVVEH GADLGIAFDG DADRVVMVDK FGNLIDGDHI LYILATQAKN KPAGVVGTVM SNMALEVALE KANVGFVRAK VGDRYVLQAL EENGWVTGGE PSGHILTLDK STTGDAIIAA LQVLTVMVEQ NKALHELVNG FKLYPQVLVN VRLEQMLDPY SIPALVAEFN KAEEQLKGRG RILIRKSGTE PVIRVMVEGD NEQEVKTLAE HLANA
Validated NT Sequence
actgcatttg ctaaatgttc agctaaagtt ttcacttctt gctcgttatc accttcaacc atgacacgaa tgactggctc agttccagac ttacgaatta aaatacgtcc acgtcctttg agctgctctt cagctttatt gaactcagca actaatgcag gaatagaata tggatcaagc atttgttcca aacgtacatt tacaagaact tgtgggtaca gtttaaagcc gttaaccaat tcgtgcagcg ctttgttctg ctcaaccatc acggttaaca cttgtaatgc tgcaataatc gcatcacctg ttgtgctctt gtctaaagtc agaatatgac cagaaggctc accacctgtt acccatccat tttcttctag ggcttgtaat acataacggt caccgacttt tgcacgaaca aagccaacat ttgctttttc aagtgccact tcaagtgcca tgttactcat taccgtacca acaacacctg ctggtttatt ttttgcctga gtcgctaaaa tatataagat gtggtcaccg tcaattaagt taccgaactt atcaaccatc actacacggt cagcatcacc atcaaaggca atacctaagt cagccccatg ttccacaacc gctttttgta agctctctgg atgagttgaa ccacagtttt cattaatatt taaaccatct ggctcattgt ataaagcgac aactttagcg ccgagttcac ggaaaaccga agggccaacg ctgtaagcag cgccgtgagc acaatccaca acaattttta aattatttaa atcaaaatga tatgggaatg tagatttaca aaattcgatg taacgaccat ttgcatcggt cacacgaaca cttttaccta agtttgcagt gtcttcgata aataaatctt tttctaattc tttattaatt tcttcctgca atgaatctgg taatttttta ccttcacttg agaagaactt aatcccgtta tcaaaatatg ggttatgtga tgcagaaata acaatccctg catgtgcatg taaggcacgg gtaagatgtg caatagccgg tgtaggtaat ggtccaagta agtgaacata tacaccagca gcatttaatc ccgcctgcaa agcagactct aaaatataac cagataaacg agtatctttt cctaatacaa caagtggttt atttttcgga ctcgttcgtt ttaatacctt ccctgcagca aaaccgagtt ttaaagcaaa ctctggcgta atcggcattt gcccaaattt tccacgaata ccatccgttc caaaataact catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS YFGTDGIRGK FGQMPITPEF ALKLGFAAGK VLKRTSPKNK PLVVLGKDTR LSGYILESAL QAGLNAAGVY VHLLGPLPTP AIAHLTRALH AHAGIVISAS HNPYFDNGIK FFSSEGKKLP DSLQEEINKE LEKDLFIEDT ANLGKSVRVT DANGRYIEFC KSTFPYHFDL NNLKIVVDCA HGAAYSVGPS VFRELGAKVV ALYNEPDGLN INENCGSTHP ESLQKAVVEH GADLGIAFDG DADRVVMVDK FGNLIDGDHI LYILATQAKN KPAGVVGTVM SNMALEVALE KANVGFVRAK VGDRYVLQAL EENGWVTGGE PSGHILTLDK STTGDAIIAA LQVLTVMVEQ NKALHELVNG FKLYPQVLVN VRLEQMLDPY SIPALVAEFN KAEEQLKGRG RILIRKSGTE PVIRVMVEGD NEQEVKTLAE HLANAVRSQA QVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt attttggaac ggatggtatt cgtggaaaat ttgggcaaat gccgattacg ccagagtttg ctttaaaact cggttttgct gcagggaagg tattaaaacg aacgagtccg aaaaataaac cacttgttgt attaggaaaa gatactcgtt tatctggtta tattttagag tctgctttgc aggcgggatt aaatgctgct ggtgtatatg ttcacttact tggaccatta cctacaccgg ctattgcaca tcttacccgt gccttacatg cacatgcagg gattgttatt tctgcatcac ataacccata ttttgataac gggattaagt tcttctcaag tgaaggtaaa aaattaccag attcattgca ggaagaaatt aataaagaat tagaaaaaga tttatttatc gaagacactg caaacttagg taaaagtgtt cgtgtgaccg atgcaaatgg tcgttacatc gaattttgta aatctacatt cccatatcat tttgatttaa ataatttaaa aattgttgtg gattgtgctc acggcgctgc ttacagcgtt ggcccttcgg ttttccgtga actcggcgct aaagttgtcg ctttatacaa tgagccagat ggtttaaata ttaatgaaaa ctgtggttca actcatccag agagcttaca aaaagcggtt gtggaacatg gggctgactt aggtattgcc tttgatggtg atgctgaccg tgtagtgatg gttgataagt tcggtaactt aattgacggt gaccacatct tatatatttt agcgactcag gcaaaaaata aaccagcagg tgttgttggt acggtaatga gtaacatggc acttgaagtg gcacttgaaa aagcaaatgt tggctttgtt cgtgcaaaag tcggtgaccg ttatgtatta caagccctag aagaaaatgg atgggtaaca ggtggtgagc cttctggtca tattctgact ttagacaaga gcacaacagg tgatgcgatt attgcagcat tacaagtgtt aaccgtgatg gttgagcaga acaaagcgct gcacgaattg gttaacggct ttaaactgta cccacaagtt cttgtaaatg tacgtttgga acaaatgctt gatccatatt ctattcctgc attagttgct gagttcaata aagctgaaga gcagctcaaa ggacgtggac gtattttaat tcgtaagtct ggaactgagc cagtcattcg tgtcatggtt gaaggtgata acgagcaaga agtgaaaact ttagctgaac atttagcaaa tgcagttcgt tcacaggcac aagtcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat