AcbaC.01348.a

glutamyl-tRNA synthetase

CENTER ID: AcbaC.01348.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01348.a.B2.GE40447 truncation1 1 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.265
GenBank: AKA30064.1
PGAT: acinetobacter:ABUW_0278
RefSeq: WP_000868422.1
UniProt: A0A059ZGG1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVRTRIAPS PTGFPHVGTA YIALFNMCFA KQHGGEFILR IEDTDQLRST PESEKMILDS LRWLGLNWSE GPDVGGPHAP YRQSERMGIY KQYALELVEK GHAFYCFATA EELDQMRAEQ QARGETPKYD GRGLKLSQEE VARRLEAGEP HVIRMKVPEE GVCKFNDLLR GEVEIPWAQV DMQVLLKTDG LPTYHLANVV DDHLMEITHV LRGEEWLPSA PKHQLLYQYF GWEMPTLCHM PLLRNPDKSK LSKRKNPTSI NYYRDIGVLP EALLNYLGRM GWSMPDEREV FTLQDMMDNF DIQRVSLGGP IFDVEKLNWL NGQWIKGLTP GQLLDRLLTW KSDRSTLEDI AAAIQPRINL LSEAVNWAGF YFNHMPQITA EMFESKKLTQ EQVRQSLQFA IWRLESQFTW NNDTVSQTLM DLANQMGIKL RDFMPTFFIA IAGSTSSTPV MQSMVTLGPD LTFARLRHAL EIVGAPSKKE VKNWEKLNES LKLPKNEATS EA
NT Sequence
ATGAAAGTTC GTACTCGTAT TGCACCTTCT CCTACCGGTT TCCCACATGT TGGTACTGCC TACATCGCCC TATTCAATAT GTGTTTTGCG AAACAGCATG GCGGGGAATT CATTTTAAGA ATTGAAGATA CAGACCAGCT TCGCTCTACG CCAGAATCTG AAAAAATGAT TTTAGACTCT CTACGCTGGT TAGGCTTAAA TTGGTCTGAA GGTCCGGATG TAGGTGGTCC TCACGCTCCT TACCGCCAAT CAGAGCGCAT GGGAATTTAT AAGCAATACG CGTTAGAACT TGTTGAAAAA GGTCATGCTT TTTATTGTTT TGCTACAGCA GAAGAACTTG ACCAAATGCG TGCCGAGCAG CAAGCTCGTG GCGAAACACC AAAATACGAT GGTCGTGGTT TAAAGCTGTC TCAAGAAGAA GTAGCTCGAC GTCTTGAAGC GGGTGAACCT CATGTTATCC GTATGAAAGT GCCTGAAGAA GGCGTATGTA AATTTAACGA CTTACTCCGT GGGGAAGTTG AAATTCCTTG GGCACAAGTC GATATGCAAG TGCTTCTTAA AACAGATGGT TTACCAACCT ACCATTTAGC TAACGTTGTT GATGACCATT TAATGGAAAT CACTCATGTA TTACGTGGTG AAGAATGGCT TCCATCTGCA CCGAAGCATC AATTGCTTTA CCAATACTTT GGTTGGGAAA TGCCAACGCT TTGCCATATG CCGTTGCTTC GTAATCCAGA TAAATCGAAG TTATCTAAAC GCAAAAACCC GACCTCAATT AACTACTACC GTGATATCGG CGTTTTACCA GAAGCCTTAC TCAACTATTT AGGCCGTATG GGTTGGTCAA TGCCGGATGA AAGAGAAGTT TTCACATTAC AAGACATGAT GGATAACTTT GATATTCAAC GCGTATCACT AGGTGGACCA ATTTTTGACG TTGAAAAACT CAATTGGTTA AATGGTCAAT GGATTAAAGG GTTAACCCCA GGACAACTAT TAGATCGTTT ATTGACATGG AAAAGTGATC GCAGCACTTT AGAAGACATT GCTGCCGCTA TTCAACCACG TATTAATTTA CTTTCTGAAG CTGTAAATTG GGCTGGTTTC TACTTTAACC ATATGCCACA AATTACGGCT GAAATGTTTG AAAGTAAAAA ATTGACTCAA GAGCAAGTTC GTCAAAGTTT ACAATTCGCA ATTTGGCGTC TTGAAAGTCA GTTTACTTGG AATAACGACA CAGTTAGTCA AACTTTAATG GATTTGGCAA ATCAGATGGG CATTAAATTA CGTGACTTCA TGCCAACGTT CTTTATTGCA ATTGCTGGCT CAACAAGTTC AACACCAGTT ATGCAGTCAA TGGTTACGCT TGGCCCTGAC TTAACCTTTG CTCGCTTACG ACATGCACTA GAAATTGTAG GTGCACCGAG TAAAAAAGAG GTAAAAAACT GGGAAAAGCT TAATGAAAGT TTAAAATTGC CGAAAAATGA AGCAACTAGC GAAGCTTAA
Details for AcbaC.01348.a.B2.GE40447
HARVESTED ON: 8/10/2016
SEQUENCED ON: 8/11/2016
EXPECTED MW: 55kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK VRTRIAPSPT GFPHVGTAYI ALFNMCFAKQ HGGEFILRIE DTDQLRSTPE SEKMILDSLR WLGLNWSEGP DVGGPHAPYR QSERMGIYKQ YALELVEKGH AFYCFATAEE LDQMRAEQQA RGETPKYDGR GLKLSQEEVA RRLEAGEPHV IRMKVPEEGV CKFNDLLRGE VEIPWAQVDM QVLLKTDGLP TYHLANVVDD HLMEITHVLR GEEWLPSAPK HQLLYQYFGW EMPTLCHMPL LRNPDKSKLS KRKNPTSINY YRDIGVLPEA LLNYLGRMGW SMPDEREVFT LQDMMDNFDI QRVSLGGPIF DVEKLNWLNG QWIKGLTPGQ LLDRLLTWKS DRSTLEDIAA AIQPRINLLS EAVNWAGFYF NHMPQITAEM FESKKLTQEQ VRQSLQFAIW RLESQFTWNN DTVSQTLMDL ANQMGIKLRD FMPTFFIAIA GSTSSTPVMQ SMVTLGPDLT FARLRHALE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag ttcgtactcg tattgcacct tctcctaccg gtttcccaca tgttggtact gcctacatcg ccctattcaa tatgtgtttt gcgaaacagc atggcgggga attcatttta agaattgaag atacagacca gcttcgctct acgccagaat ctgaaaaaat gattttagac tctctacgct ggttaggctt aaattggtct gaaggtccgg atgtaggtgg tcctcacgct ccttaccgcc aatcagagcg catgggaatt tataagcaat acgcgttaga acttgttgaa aaaggtcatg ctttttattg ttttgctaca gcagaagaac ttgaccaaat gcgtgccgag cagcaagctc gtggcgaaac accaaaatac gatggtcgtg gtttaaagct gtctcaagaa gaagtagctc gacgtcttga agcgggtgaa cctcatgtta tccgtatgaa agtgcctgaa gaaggcgtat gtaaatttaa cgacttactc cgtggggaag ttgaaattcc ttgggcacaa gtcgatatgc aagtgcttct taaaacagat ggtttaccaa cctaccattt agctaacgtt gttgatgacc atttaatgga aatcactcat gtattacgtg gtgaagaatg gcttccatct gcaccgaagc atcaattgct ttaccaatac tttggttggg aaatgccaac gctttgccat atgccgttgc ttcgtaatcc agataaatcg aagttatcta aacgcaaaaa cccgacctca attaactact accgtgatat cggcgtttta ccagaagcct tactcaacta tttaggccgt atgggttggt caatgccgga tgaaagagaa gttttcacat tacaagacat gatggataac tttgatattc aacgcgtatc actaggtgga ccaatttttg acgttgaaaa actcaattgg ttaaatggtc aatggattaa agggttaacc ccaggacaac tattagatcg tttattgaca tggaaaagtg atcgcagcac tttagaagac attgctgccg ctattcaacc acgtattaat ttactttctg aagctgtaaa ttgggctggt ttctacttta accatatgcc acaaattacg gctgaaatgt ttgaaagtaa aaaattgact caagagcaag ttcgtcaaag tttacaattc gcaatttggc gtcttgaaag tcagtttact tggaataacg acacagttag tcaaacttta atggatttgg caaatcagat gggcattaaa ttacgtgact tcatgccaac gttctttatt gcaattgctg gctcaacaag ttcaacacca gttatgcagt caatggttac gcttggccct gacttaacct ttgctcgctt acgacatgca ctagaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t