AcbaC.01331.a
phosphoglycerate kinase
| CENTER ID: | AcbaC.01331.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01331.a.B1.GE38686 | full length | 1 | 395 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5BT8
DEPOSITED: 6/2/2015
DETERMINATION: XRay
CLONE: AcbaC.01331.a.B1.GE38686
PROTEIN: AcbaC.01331.a.B1.PS02354
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|470.1345.peg.2226 |
| GenBank: | AKA31999.1 |
| PGAT: | acinetobacter:ABUW_2271 |
| RefSeq: | WP_001011093.1 |
| UniProt: | A0A086HXS4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFQRMTDLN LAGKRVLIRE DLNVPVKNGV ITSDARLRAA LPTIKAALEK GAAVMVFSHL GRPVEGEPKP EQSLAPVAAY LTEALGQEVK LFTDYLDGVE VEAGQVVLLE NVRFNPGEKK NNPELAQKYA ALCDVFVMDA FGTAHRAEAS TEGVARFAPV AAAGPLLAAE LDALGRAMQT PEKPMVAIVA GSKVSTKLDV LNSLSGICDQ LIVGGGIANT FLAAAGYNVG KSLYEADLVE TAKQIAAKVS VPLPTDVVVA DASQINFEDF LGSLAAAQAV IKKVEDVTAN DMILDVGPET AKAFANILTT SKTILWNGPV GVFEVDQFGE GTKALSLAVA QSDAFSIAGG GDTLAAIDKY NVADQIGYIS TGGGAFLEFV EGKTLPAVAV LLERA
NT Sequence
ATGAATTTTC AGCGCATGAC TGACCTTAAT TTAGCAGGTA AACGTGTCCT TATTCGTGAA GATTTAAACG TTCCTGTTAA AAATGGTGTG ATTACCAGCG ATGCTCGTTT ACGTGCCGCA CTTCCAACAA TAAAAGCTGC TTTAGAAAAA GGCGCTGCTG TAATGGTATT TTCACATTTA GGTCGTCCTG TGGAAGGTGA GCCAAAGCCT GAACAATCAC TTGCACCAGT GGCTGCATAT TTAACTGAAG CATTAGGTCA AGAAGTTAAA TTATTTACTG ATTATTTAGA TGGTGTTGAA GTTGAAGCAG GACAAGTTGT TTTGCTTGAA AATGTTCGTT TTAATCCGGG TGAAAAGAAA AATAATCCTG AACTTGCACA AAAATATGCA GCTCTATGTG ATGTGTTTGT AATGGATGCT TTTGGTACTG CTCACCGTGC AGAAGCTTCT ACAGAAGGTG TAGCACGTTT TGCACCTGTT GCAGCGGCAG GCCCATTATT GGCTGCTGAA TTAGATGCAC TTGGCCGTGC AATGCAAACA CCTGAAAAAC CAATGGTGGC AATTGTCGCT GGTTCTAAAG TTTCAACAAA ACTTGATGTA TTAAATTCAT TATCTGGTAT TTGTGACCAA CTGATTGTGG GAGGTGGTAT TGCAAATACT TTCTTGGCGG CTGCTGGTTA CAATGTTGGT AAATCTTTAT ATGAAGCTGA CTTGGTTGAA ACTGCAAAAC AAATTGCTGC TAAAGTAAGT GTTCCACTTC CTACAGACGT AGTTGTAGCT GATGCCTCAC AAATTAACTT CGAAGATTTC TTAGGTTCTT TGGCAGCAGC GCAAGCAGTA ATTAAGAAAG TTGAAGATGT CACAGCAAAT GATATGATTT TGGATGTGGG TCCTGAAACA GCTAAAGCAT TTGCAAATAT TTTAACAACT TCAAAAACGA TTCTTTGGAA TGGTCCTGTT GGTGTATTTG AAGTTGATCA ATTTGGTGAA GGTACTAAAG CACTTTCTCT TGCAGTTGCA CAATCTGATG CATTCTCAAT TGCTGGTGGT GGTGATACTT TAGCTGCGAT CGATAAATAT AATGTAGCTG ATCAAATTGG TTATATTTCA ACAGGTGGTG GTGCATTCCT TGAATTTGTT GAAGGAAAAA CATTGCCAGC AGTTGCCGTT TTACTTGAAC GTGCTTAA
Details for AcbaC.01331.a.B1.GE38686
| HARVESTED ON: | 12/9/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMN FQRMTDLNLA GKRVLIREDL NVPVKNGVIT SDARLRAALP TIKAALEKGA AVMVFSHLGR PVEGEPKPEQ SLAPVAAYLT EALGQEVKLF TDYLDGVEVE AGQVVLLENV RFNPGEKKNN PELAQKYAAL CDVFVMDAFG TAHRAEASTE GVARFAPVAA AGPLLAAELD ALGRAMQTPE KPMVAIVAGS KVSTKLDVLN SLSGICDQLI VGGGIANTFL AAAGYNVGKS LYEADLVETA KQIAAKVSVP LPTDVVVADA SQINFEDFLG SLAAAQAVIK KVEDVTANDM ILDVGPETAK AFANILTTSK TILWNGPVGV FEVDQFGEGT KALSLAVAQS DAFSIAGGGD TLAAIDKYNV ADQIGYISTG GGAFLEFVEG KTLPA
Validated NT Sequence
ctgctggcaa tgtttttcct tcaacaaatt caaggaatgc accaccacct gttgaaatat aaccaatttg atcagctaca ttatatttat cgatcgcagc taaagtatca ccaccaccag caattgagaa tgcatcagat tgtgcaactg caagagaaag tgctttagta ccttcaccaa attgatcaac ttcaaataca ccaacaggac cattccaaag aatcgttttt gaagttgtta aaatatttgc aaatgcttta gctgtttcag gacccacatc caaaatcata tcatttgctg tgacatcttc aactttctta attactgctt gcgctgctgc caaagaacct aagaaatctt cgaagttaat ttgtgaggca tcagctacaa ctacgtctgt aggaagtgga acacttactt tagcagcaat ttgttttgca gtttcaacca agtcagcttc atataaagat ttaccaacat tgtaaccagc agccgccaag aaagtatttg caataccacc tcccacaatc agttggtcac aaataccaga taatgaattt aatacatcaa gttttgttga aactttagaa ccagcgacaa ttgccaccat tggtttttca ggtgtttgca ttgcacggcc aagtgcatct aattcagcag ccaataatgg gcctgccgct gcaacaggtg caaaacgtgc tacaccttct gtagaagctt ctgcacggtg agcagtacca aaagcatcca ttacaaacac atcacataga gctgcatatt tttgtgcaag ttcaggatta tttttctttt cacccggatt aaaacgaaca ttttcaagca aaacaacttg tcctgcttca acttcaacac catctaaata atcagtaaat aatttaactt cttgacctaa tgcttcagtt aaatatgcag ccactggtgc aagtgattgt tcaggctttg gctcaccttc cacaggacga cctaaatgtg aaaataccat tacagcagcg cctttttcta aagcagcttt tattgttgga agtgcggcac gtaaacgagc atcgctggta atcacaccat ttttaacagg aacgtttaaa tcttcacgaa taaggacacg tttacctgct aaattaaggt cagtcatgcg ctgaaaattc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN FQRMTDLNLA GKRVLIREDL NVPVKNGVIT SDARLRAALP TIKAALEKGA AVMVFSHLGR PVEGEPKPEQ SLAPVAAYLT EALGQEVKLF TDYLDGVEVE AGQVVLLENV RFNPGEKKNN PELAQKYAAL CDVFVMDAFG TAHRAEASTE GVARFAPVAA AGPLLAAELD ALGRAMQTPE KPMVAIVAGS KVSTKLDVLN SLSGICDQLI VGGGIANTFL AAAGYNVGKS LYEADLVETA KQIAAKVSVP LPTDVVVADA SQINFEDFLG SLAAAQAVIK KVEDVTANDM ILDVGPETAK AFANILTTSK TILWNGPVGV FEVDQFGEGT KALSLAVAQS DAFSIAGGGD TLAAIDKYNV ADQIGYISTG GGAFLEFVEG KTLPAVAVLL ERA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt ttcagcgcat gactgacctt aatttagcag gtaaacgtgt ccttattcgt gaagatttaa acgttcctgt taaaaatggt gtgattacca gcgatgctcg tttacgtgcc gcacttccaa caataaaagc tgctttagaa aaaggcgctg ctgtaatggt attttcacat ttaggtcgtc ctgtggaagg tgagccaaag cctgaacaat cacttgcacc agtggctgca tatttaactg aagcattagg tcaagaagtt aaattattta ctgattattt agatggtgtt gaagttgaag caggacaagt tgttttgctt gaaaatgttc gttttaatcc gggtgaaaag aaaaataatc ctgaacttgc acaaaaatat gcagctctat gtgatgtgtt tgtaatggat gcttttggta ctgctcaccg tgcagaagct tctacagaag gtgtagcacg ttttgcacct gttgcagcgg caggcccatt attggctgct gaattagatg cacttggccg tgcaatgcaa acacctgaaa aaccaatggt ggcaattgtc gctggttcta aagtttcaac aaaacttgat gtattaaatt cattatctgg tatttgtgac caactgattg tgggaggtgg tattgcaaat actttcttgg cggctgctgg ttacaatgtt ggtaaatctt tatatgaagc tgacttggtt gaaactgcaa aacaaattgc tgctaaagta agtgttccac ttcctacaga cgtagttgta gctgatgcct cacaaattaa cttcgaagat ttcttaggtt ctttggcagc agcgcaagca gtaattaaga aagttgaaga tgtcacagca aatgatatga ttttggatgt gggtcctgaa acagctaaag catttgcaaa tattttaaca acttcaaaaa cgattctttg gaatggtcct gttggtgtat ttgaagttga tcaatttggt gaaggtacta aagcactttc tcttgcagtt gcacaatctg atgcattctc aattgctggt ggtggtgata ctttagctgc gatcgataaa tataatgtag ctgatcaaat tggttatatt tcaacaggtg gtggtgcatt ccttgaattt gttgaaggaa aaacattgcc agcagttgcc gttttacttg aacgtgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat