AcbaC.01294.a

transketolase

CENTER ID: AcbaC.01294.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01294.a.B1.GE38685 full length 1 662
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2250
GenBank: AKA32023.1
PGAT: acinetobacter:ABUW_2295
RefSeq: WP_000211068.1
UniProt: V5XVR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTPLNERRI ANAIRVLAMD AVQQANSGHP GAPMGMADIA DVVWREFLNH NPNNPQWANR DRFVLSNGHG SMLQYALLHL TGYDLSIEDL KQFRQLHSKT PGHPEYGYAP GIETTTGPLG QGIANAVGFA LAEKTLAAQF NKDDLKVVDH FTYCFLGDGC LMEGISHEVC SLAGTLQLGK LIAYYDDNGI SIDGEVEGWF SDDTEERFKS YGWQVLRVDG HDADAIRQAT VEAKAETQKP TIIICKTIIG LGSPNKQGKE DCHGAPLGKD EITLTREALG WTEEAFVIPA DVYAAWDAKA KGNEAEAAWN EVFAQYQAKY PTEAAELLRR ISGDLPAEFS AQADAFIRET NAKAETVATR KASQNTLQAF GPLLPELLGG SADLAGSNLT LWKGCQGVQE NPAGNYVYYG VREFGMTAIA NGVALHGGFI PYVATFLMFM EYARNAVRMS ALMKQRVIHV YTHDSIGLGE DGPTHQPIEQ IASLRGTPNL NTWRPADTVE TAIAWKSALE RKDGPTALIF SRQNLPFQTR TEEQIQNAAK GGYVLAQEKG ELKAIIIATG SEVSLAMEAY AQLEGVRVVS MPCAEEFMKQ DAAYREAVLP AHIRARVAVE AAHVDYWWKF VGLDGKVIGM TTYGESAPAK DLFQFFGITT EAVVAAVKEL TA
NT Sequence
ATGACGACCC CTTTAAACGA ACGTCGTATT GCAAACGCAA TTCGTGTATT GGCAATGGAT GCTGTGCAAC AGGCAAACTC AGGGCATCCG GGTGCTCCAA TGGGGATGGC AGATATTGCT GATGTCGTCT GGCGCGAGTT TTTAAATCAC AACCCAAATA ATCCACAATG GGCAAACCGT GACCGTTTTG TTTTATCAAA TGGCCATGGT TCAATGTTGC AATATGCTTT GCTTCATTTA ACAGGTTATG ATCTTTCAAT TGAAGATTTA AAACAATTCC GTCAATTGCA CTCAAAAACT CCTGGTCACC CTGAATATGG TTATGCTCCA GGAATTGAAA CAACGACAGG TCCTTTGGGT CAAGGTATTG CGAACGCCGT TGGTTTTGCA TTAGCAGAAA AAACTTTAGC TGCTCAGTTC AATAAAGATG ACTTAAAAGT AGTTGATCAC TTTACCTATT GTTTCTTAGG TGATGGCTGT TTAATGGAAG GTATTTCTCA TGAAGTATGT TCTTTAGCAG GTACTTTACA GCTTGGTAAA TTGATTGCTT ATTATGATGA CAACGGCATT TCGATTGATG GTGAAGTAGA AGGCTGGTTC AGTGATGACA CTGAAGAGCG TTTCAAGTCT TATGGTTGGC AAGTTCTTCG TGTAGATGGC CACGATGCTG ATGCGATTCG TCAAGCAACA GTTGAAGCTA AAGCAGAAAC TCAAAAACCA ACGATTATTA TTTGTAAAAC AATTATTGGC TTAGGTTCTC CAAACAAGCA AGGTAAAGAA GACTGTCACG GTGCACCGCT GGGTAAAGAT GAAATTACCT TAACTCGTGA AGCTTTAGGC TGGACTGAAG AAGCGTTTGT TATTCCTGCT GATGTATATG CTGCATGGGA TGCTAAAGCA AAAGGTAATG AAGCTGAAGC AGCTTGGAAT GAAGTATTTG CTCAATACCA AGCAAAATAT CCAACGGAAG CGGCTGAGTT ACTTCGTCGT ATTAGCGGTG ACTTACCAGC AGAATTTTCA GCTCAAGCAG ATGCATTTAT TCGTGAAACA AATGCTAAAG CTGAGACTGT AGCAACTCGT AAAGCGAGCC AAAACACATT ACAAGCATTT GGTCCTTTAT TACCTGAATT ATTAGGTGGT TCTGCGGACT TGGCAGGTTC TAACTTAACA CTTTGGAAAG GTTGCCAAGG CGTTCAAGAA AACCCGGCTG GTAACTACGT ATATTATGGT GTACGTGAGT TCGGTATGAC TGCAATCGCT AACGGTGTTG CATTGCATGG CGGCTTCATT CCTTACGTTG CAACGTTCTT AATGTTTATG GAATATGCAC GTAATGCGGT ACGTATGTCT GCATTAATGA AACAGCGCGT GATTCATGTG TATACACATG ACTCAATCGG TTTAGGTGAA GATGGTCCAA CACACCAACC AATTGAACAA ATTGCTTCTT TACGTGGCAC ACCAAACTTA AATACTTGGC GTCCTGCTGA TACTGTAGAA ACAGCAATTG CGTGGAAATC TGCTTTAGAG CGTAAAGATG GCCCTACAGC ACTTATTTTC TCTCGACAAA ACTTACCATT CCAAACGCGT ACTGAAGAGC AAATCCAAAA TGCGGCAAAA GGTGGTTATG TTCTTGCTCA AGAGAAAGGT GAGTTGAAAG CAATTATTAT TGCTACTGGT TCAGAAGTTT CTTTAGCAAT GGAAGCTTAT GCTCAACTTG AAGGTGTTCG TGTTGTTTCT ATGCCATGTG CAGAAGAGTT CATGAAACAA GATGCTGCAT ATCGTGAAGC AGTATTACCA GCTCACATTC GTGCACGTGT TGCTGTAGAA GCAGCTCACG TTGACTACTG GTGGAAGTTT GTTGGTCTTG ATGGCAAAGT AATTGGTATG ACAACTTATG GTGAGTCAGC ACCAGCAAAA GACTTGTTCC AATTCTTCGG TATTACAACT GAGGCTGTAG TTGCTGCGGT GAAAGAGTTA ACTGCTTAA
Details for AcbaC.01294.a.B1.GE38685
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 73kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT TPLNERRIAN AIRVLAMDAV QQANSGHPGA PMGMADIADV VWREFLNHNP NNPQWANRDR FVLSNGHGSM LQYALLHLTG YDLSIEDLKQ FRQLHSKTPG HPEYGYAPGI ETTTGPLGQG IANAVGFALA EKTLAAQFNK DDLKVVDHFT YCFLGDGCLM EGISHEVCSL AGTLQLGKLI AYYDDNGISI DGEVEGWFSD DTEERFKSYG WQVLRVDGHD ADAIRQATVE AKAETQKPTI IICKTIIGLG SPNKQGKEDC HGAPLGKDEI TLTREALGWT EEAFVIPADV YAAWDAKAKG NEAEAAWNEV FAQYQAKYPT EAAELLRRIS GDLPAEFSAQ ADAFIRETNA KAETVATRKA SQNTLQAFGP LLPELLGGSA DLAGSNLTLW KGCQGVQENP AGNYVYYGVR EFGMTAIANG VALHGGFIPY VATFLMFMEY ARNAVRMSAL MKQRVIHVYT HDSIGLGEDG PTHQPIEQIA SLRGTPNLNT WRPADTVETA IAWKSALERK DGPTALIFSR QNLPFQTRTE EQIQNAAKGG YVLAQEKGEL KAIIIATGSE VSLAMEAYAQ LEGVRVVSMP CAEEFMKQDA AYREAVLPAH IRARVAVEAA HVDYWWKFVG LDGKVIGMTT YGESAPAKDL FQFFGITTEA VVAAVKELTA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cccctttaaa cgaacgtcgt attgcaaacg caattcgtgt attggcaatg gatgctgtgc aacaggcaaa ctcagggcat ccgggtgctc caatggggat ggcagatatt gctgatgtcg tctggcgcga gtttttaaat cacaacccaa ataatccaca atgggcaaac cgtgaccgtt ttgttttatc aaatggccat ggttcaatgt tgcaatatgc tttgcttcat ttaacaggtt atgatctttc aattgaagat ttaaaacaat tccgtcaatt gcactcaaaa actcctggtc accctgaata tggttatgct ccaggaattg aaacaacgac aggtcctttg ggtcaaggta ttgcgaacgc cgttggtttt gcattagcag aaaaaacttt agctgctcag ttcaataaag atgacttaaa agtagttgat cactttacct attgtttctt aggtgatggc tgtttaatgg aaggtatttc tcatgaagta tgttctttag caggtacttt acagcttggt aaattgattg cttattatga tgacaacggc atttcgattg atggtgaagt agaaggctgg ttcagtgatg acactgaaga gcgtttcaag tcttatggtt ggcaagttct tcgtgtagat ggccacgatg ctgatgcgat tcgtcaagca acagttgaag ctaaagcaga aactcaaaaa ccaacgatta ttatttgtaa aacaattatt ggcttaggtt ctccaaacaa gcaaggtaaa gaagactgtc acggtgcacc gctgggtaaa gatgaaatta ccttaactcg tgaagcttta ggctggactg aagaagcgtt tgttattcct gctgatgtat atgctgcatg ggatgctaaa gcaaaaggta atgaagctga agcagcttgg aatgaagtat ttgctcaata ccaagcaaaa tatccaacgg aagcggctga gttacttcgt cgtattagcg gtgacttacc agcagaattt tcagctcaag cagatgcatt tattcgtgaa acaaatgcta aagctgagac tgtagcaact cgtaaagcga gccaaaacac attacaagca tttggtcctt tattacctga attattaggt ggttctgcgg acttggcagg ttctaactta acactttgga aaggttgcca aggcgttcaa gaaaacccgg ctggtaacta cgtatattat ggtgtacgtg agttcggtat gactgcaatc gctaacggtg ttgcattgca tggcggcttc attccttacg ttgcaacgtt cttaatgttt atggaatatg cacgtaatgc ggtacgtatg tctgcattaa tgaaacagcg cgtgattcat gtgtatacac atgactcaat cggtttaggt gaagatggtc caacacacca accaattgaa caaattgctt ctttacgtgg cacaccaaac ttaaatactt ggcgtcctgc tgatactgta gaaacagcaa ttgcgtggaa atctgcttta gagcgtaaag atggccctac agcacttatt ttctctcgac aaaacttacc attccaaacg cgtactgaag agcaaatcca aaatgcggca aaaggtggtt atgttcttgc tcaagagaaa ggtgagttga aagcaattat tattgctact ggttcagaag tttctttagc aatggaagct tatgctcaac ttgaaggtgt tcgtgttgtt tctatgccat gtgcagaaga gttcatgaaa caagatgctg catatcgtga agcagtatta ccagctcaca ttcgtgcacg tgttgctgta gaagcagctc acgttgacta ctggtggaag tttgttggtc ttgatggcaa agtaattggt atgacaactt atggtgagtc agcaccagca aaagacttgt tccaattctt cggtattaca actgaggctg tagttgctgc ggtgaaagag ttaactgctt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat