AcbaC.01238.a

seryl-tRNA synthetase

CENTER ID: AcbaC.01238.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01238.a.B1.GE38683 full length 1 423
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01238.a.B1.PW37672 full length 1 423

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.818
GenBank: AKA30612.1
PGAT: acinetobacter:ABUW_0852
RefSeq: WP_000566842.1
UniProt: A0A0D5YE82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDPKLLRNN IEVVNAGLAK RGVQLDVQEW ASLETRRKDL QSKTEKLQAE RNAGAKQVGQ IKKSGGDASE IMARMQAIGD EIKAAEVALS ELQNEIEQKA LSIPNLPDES VPAGKDENDN VEISKWGTPR QFDFEIKDHT DLGEWMGGLE FETATKLTGS RFSVLKGPLA RLQRALTQFM LDTHTLKNGY TEAYVPYLVN ADSLRGTGQL PKFEEDLFKL QGEKEYYLIP TAEVPVTNFV RDEIIDADRL PLKYAAHTPC FRSEAGSYGR DTRGLIRQHQ FDKVEMVQIV KPETSMQALE ELTAHAEGIL QALGLPYRKI LLCGGDMGFG ATKTYDLEVW VPSQNTYREI SSCSNMGDFQ ARRMKARYRM DQKKTELVHT LNGSGLAVGR TLLAVMENYQ REDGSIEIPE VLRPYMGGAT FID
NT Sequence
ATGATCGACC CTAAGTTACT CAGAAATAAT ATTGAAGTCG TTAATGCAGG TTTGGCAAAA CGTGGTGTTC AGTTAGATGT TCAAGAATGG GCATCTTTAG AGACTCGCCG TAAAGACTTG CAATCAAAAA CTGAAAAGTT ACAGGCAGAG CGAAATGCCG GTGCTAAACA AGTGGGTCAG ATTAAAAAAT CCGGTGGTGA TGCTTCTGAA ATCATGGCAC GTATGCAAGC CATTGGTGAT GAAATTAAAG CTGCCGAAGT TGCTTTATCA GAGCTACAAA ACGAGATTGA GCAAAAAGCG CTTAGCATTC CTAACTTGCC GGACGAATCT GTACCAGCTG GTAAAGATGA AAATGATAAC GTTGAAATCT CAAAATGGGG TACACCTCGT CAGTTCGATT TTGAAATCAA AGATCATACT GATTTAGGTG AGTGGATGGG CGGACTGGAG TTTGAAACTG CCACTAAACT CACAGGCTCA CGTTTTAGTG TGTTGAAGGG ACCTTTAGCG CGTCTACAAC GTGCTTTAAC TCAGTTCATG CTCGATACCC ATACTTTAAA GAATGGTTAT ACCGAAGCGT ATGTGCCTTA TCTGGTAAAT GCTGATTCAT TACGTGGGAC AGGTCAGTTA CCAAAATTTG AAGAAGATTT ATTTAAACTT CAAGGCGAAA AAGAATACTA CCTCATCCCT ACAGCAGAAG TTCCAGTCAC TAACTTTGTC CGTGATGAAA TTATTGATGC AGACCGTTTA CCATTAAAAT ATGCGGCTCA TACACCATGT TTCCGTAGTG AAGCGGGTTC TTATGGTCGT GATACACGTG GTTTAATTCG TCAGCACCAA TTTGATAAAG TTGAAATGGT GCAAATCGTT AAGCCTGAAA CATCTATGCA GGCACTTGAA GAGCTTACTG CACATGCTGA GGGCATTTTA CAGGCGCTTG GTTTGCCTTA CCGCAAAATT TTGCTCTGTG GTGGTGATAT GGGCTTCGGT GCGACTAAAA CTTATGATTT AGAAGTTTGG GTTCCAAGTC AAAATACTTA CCGTGAAATT TCAAGCTGCT CTAATATGGG TGATTTCCAG GCACGTCGTA TGAAAGCACG CTACCGTATG GATCAGAAGA AGACCGAATT GGTGCACACT CTAAATGGTT CAGGCCTTGC TGTAGGGCGT ACTTTACTTG CAGTGATGGA AAACTACCAA CGCGAAGACG GTTCAATTGA GATTCCAGAA GTATTACGCC CATATATGGG TGGTGCAACG TTTATTGATT AA
Details for AcbaC.01238.a.B1.GE38683
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI DPKLLRNNIE VVNAGLAKRG VQLDVQEWAS LETRRKDLQS KTEKLQAERN AGAKQVGQIK KSGGDASEIM ARMQAIGDEI KAAEVALSEL QNEIEQKALS IPNLPDESVP AGKDENDNVE ISKWGTPRQF DFEIKDHTDL GEWMGGLEFE TATKLTGSRF SVLKGPLARL QRALTQFMLD THTLKNGYTE AYVPYLVNAD SLRGTGQLPK FEEDLFKLQG EKEYYLIPTA EVPVTNFVRD EIIDADRLPL KYAAHTPCFR SEAGSYGRDT RGLIRQHQFD KVEMVQIVKP ETSMQALEEL TAHAEGILQA LGLPYRKILL CGGDMGFGAT KTYDLEVWVP SQNTYREISS CSNMGDFQAR RMKARYRMDQ KKTELVHTLN GSGLAVGRTL LAVMENYQRE DGSIEIPEVL RPY
Validated NT Sequence
catatatggg cgtaatactt ctggaatctc aattgaaccg tcttcgcgtt ggtagttttc catcactgca agtaaagtac gccctacagc aaggcctgaa ccatttagag tgtgcaccaa ttcggtcttc ttctgatcca tacggtagcg tgctttcata cgacgtgcct ggaaatcacc catattagag cagcttgaaa tttcacggta agtattttga cttggaaccc aaacttctaa atcataagtt ttagtcgcac cgaagcccat atcaccacca cagagcaaaa ttttgcggta aggcaaacca agcgcctgta aaatgccctc agcatgtgca gtaagctctt caagtgcctg catagatgtt tcaggcttaa cgatttgcac catttcaact ttatcaaatt ggtgctgacg aattaaacca cgtgtatcac gaccataaga acccgcttca ctacggaaac atggtgtatg agccgcatat tttaatggta aacggtctgc atcaataatt tcatcacgga caaagttagt gactggaact tctgctgtag ggatgaggta gtattctttt tcgccttgaa gtttaaataa atcttcttca aattttggta actgacctgt cccacgtaat gaatcagcat ttaccagata aggcacatac gcttcggtat aaccattctt taaagtatgg gtatcgagca tgaactgagt taaagcacgt tgtagacgcg ctaaaggtcc cttcaacaca ctaaaacgtg agcctgtgag tttagtggca gtttcaaact ccagtccgcc catccactca cctaaatcag tatgatcttt gatttcaaaa tcgaactgac gaggtgtacc ccattttgag atttcaacgt tatcattttc atctttacca gctggtacag attcgtccgg caagttagga atgctaagcg ctttttgctc aatctcgttt tgtagctctg ataaagcaac ttcggcagct ttaatttcat caccaatggc ttgcatacgt gccatgattt cagaagcatc accaccggat tttttaatct gacccacttg tttagcaccg gcatttcgct ctgcctgtaa cttttcagtt tttgattgca agtctttacg gcgagtctct aaagatgccc attcttgaac atctaactga acaccacgtt ttgccaaacc tgcattaacg acttcaatat tatttctgag taacttaggg tcgatcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMI DPKLLRNNIE VVNAGLAKRG VQLDVQEWAS LETRRKDLQS KTEKLQAERN AGAKQVGQIK KSGGDASEIM ARMQAIGDEI KAAEVALSEL QNEIEQKALS IPNLPDESVP AGKDENDNVE ISKWGTPRQF DFEIKDHTDL GEWMGGLEFE TATKLTGSRF SVLKGPLARL QRALTQFMLD THTLKNGYTE AYVPYLVNAD SLRGTGQLPK FEEDLFKLQG EKEYYLIPTA EVPVTNFVRD EIIDADRLPL KYAAHTPCFR SEAGSYGRDT RGLIRQHQFD KVEMVQIVKP ETSMQALEEL TAHAEGILQA LGLPYRKILL CGGDMGFGAT KTYDLEVWVP SQNTYREISS CSNMGDFQAR RMKARYRMDQ KKTELVHTLN GSGLAVGRTL LAVMENYQRE DGSIEIPEVL RPYMGGATFI D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg accctaagtt actcagaaat aatattgaag tcgttaatgc aggtttggca aaacgtggtg ttcagttaga tgttcaagaa tgggcatctt tagagactcg ccgtaaagac ttgcaatcaa aaactgaaaa gttacaggca gagcgaaatg ccggtgctaa acaagtgggt cagattaaaa aatccggtgg tgatgcttct gaaatcatgg cacgtatgca agccattggt gatgaaatta aagctgccga agttgcttta tcagagctac aaaacgagat tgagcaaaaa gcgcttagca ttcctaactt gccggacgaa tctgtaccag ctggtaaaga tgaaaatgat aacgttgaaa tctcaaaatg gggtacacct cgtcagttcg attttgaaat caaagatcat actgatttag gtgagtggat gggcggactg gagtttgaaa ctgccactaa actcacaggc tcacgtttta gtgtgttgaa gggaccttta gcgcgtctac aacgtgcttt aactcagttc atgctcgata cccatacttt aaagaatggt tataccgaag cgtatgtgcc ttatctggta aatgctgatt cattacgtgg gacaggtcag ttaccaaaat ttgaagaaga tttatttaaa cttcaaggcg aaaaagaata ctacctcatc cctacagcag aagttccagt cactaacttt gtccgtgatg aaattattga tgcagaccgt ttaccattaa aatatgcggc tcatacacca tgtttccgta gtgaagcggg ttcttatggt cgtgatacac gtggtttaat tcgtcagcac caatttgata aagttgaaat ggtgcaaatc gttaagcctg aaacatctat gcaggcactt gaagagctta ctgcacatgc tgagggcatt ttacaggcgc ttggtttgcc ttaccgcaaa attttgctct gtggtggtga tatgggcttc ggtgcgacta aaacttatga tttagaagtt tgggttccaa gtcaaaatac ttaccgtgaa atttcaagct gctctaatat gggtgatttc caggcacgtc gtatgaaagc acgctaccgt atggatcaga agaagaccga attggtgcac actctaaatg gttcaggcct tgctgtaggg cgtactttac ttgcagtgat ggaaaactac caacgcgaag acggttcaat tgagattcca gaagtattac gcccatatat gggtggtgca acgtttattg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for AcbaC.01238.a.B1.PW37672
PURIFICATION DATe: 3/10/2015
CONCENTRATION: 33mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI DPKLLRNNIE VVNAGLAKRG VQLDVQEWAS LETRRKDLQS KTEKLQAERN AGAKQVGQIK KSGGDASEIM ARMQAIGDEI KAAEVALSEL QNEIEQKALS IPNLPDESVP AGKDENDNVE ISKWGTPRQF DFEIKDHTDL GEWMGGLEFE TATKLTGSRF SVLKGPLARL QRALTQFMLD THTLKNGYTE AYVPYLVNAD SLRGTGQLPK FEEDLFKLQG EKEYYLIPTA EVPVTNFVRD EIIDADRLPL KYAAHTPCFR SEAGSYGRDT RGLIRQHQFD KVEMVQIVKP ETSMQALEEL TAHAEGILQA LGLPYRKILL CGGDMGFGAT KTYDLEVWVP SQNTYREISS CSNMGDFQAR RMKARYRMDQ KKTELVHTLN GSGLAVGRTL LAVMENYQRE DGSIEIPEVL RPY
Validated NT Sequence
catatatggg cgtaatactt ctggaatctc aattgaaccg tcttcgcgtt ggtagttttc catcactgca agtaaagtac gccctacagc aaggcctgaa ccatttagag tgtgcaccaa ttcggtcttc ttctgatcca tacggtagcg tgctttcata cgacgtgcct ggaaatcacc catattagag cagcttgaaa tttcacggta agtattttga cttggaaccc aaacttctaa atcataagtt ttagtcgcac cgaagcccat atcaccacca cagagcaaaa ttttgcggta aggcaaacca agcgcctgta aaatgccctc agcatgtgca gtaagctctt caagtgcctg catagatgtt tcaggcttaa cgatttgcac catttcaact ttatcaaatt ggtgctgacg aattaaacca cgtgtatcac gaccataaga acccgcttca ctacggaaac atggtgtatg agccgcatat tttaatggta aacggtctgc atcaataatt tcatcacgga caaagttagt gactggaact tctgctgtag ggatgaggta gtattctttt tcgccttgaa gtttaaataa atcttcttca aattttggta actgacctgt cccacgtaat gaatcagcat ttaccagata aggcacatac gcttcggtat aaccattctt taaagtatgg gtatcgagca tgaactgagt taaagcacgt tgtagacgcg ctaaaggtcc cttcaacaca ctaaaacgtg agcctgtgag tttagtggca gtttcaaact ccagtccgcc catccactca cctaaatcag tatgatcttt gatttcaaaa tcgaactgac gaggtgtacc ccattttgag atttcaacgt tatcattttc atctttacca gctggtacag attcgtccgg caagttagga atgctaagcg ctttttgctc aatctcgttt tgtagctctg ataaagcaac ttcggcagct ttaatttcat caccaatggc ttgcatacgt gccatgattt cagaagcatc accaccggat tttttaatct gacccacttg tttagcaccg gcatttcgct ctgcctgtaa cttttcagtt tttgattgca agtctttacg gcgagtctct aaagatgccc attcttgaac atctaactga acaccacgtt ttgccaaacc tgcattaacg acttcaatat tatttctgag taacttaggg tcgatcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMI DPKLLRNNIE VVNAGLAKRG VQLDVQEWAS LETRRKDLQS KTEKLQAERN AGAKQVGQIK KSGGDASEIM ARMQAIGDEI KAAEVALSEL QNEIEQKALS IPNLPDESVP AGKDENDNVE ISKWGTPRQF DFEIKDHTDL GEWMGGLEFE TATKLTGSRF SVLKGPLARL QRALTQFMLD THTLKNGYTE AYVPYLVNAD SLRGTGQLPK FEEDLFKLQG EKEYYLIPTA EVPVTNFVRD EIIDADRLPL KYAAHTPCFR SEAGSYGRDT RGLIRQHQFD KVEMVQIVKP ETSMQALEEL TAHAEGILQA LGLPYRKILL CGGDMGFGAT KTYDLEVWVP SQNTYREISS CSNMGDFQAR RMKARYRMDQ KKTELVHTLN GSGLAVGRTL LAVMENYQRE DGSIEIPEVL RPYMGGATFI D
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCG ACCCTAAGTT ACTCAGAAAT AATATTGAAG TCGTTAATGC AGGTTTGGCA AAACGTGGTG TTCAGTTAGA TGTTCAAGAA TGGGCATCTT TAGAGACTCG CCGTAAAGAC TTGCAATCAA AAACTGAAAA GTTACAGGCA GAGCGAAATG CCGGTGCTAA ACAAGTGGGT CAGATTAAAA AATCCGGTGG TGATGCTTCT GAAATCATGG CACGTATGCA AGCCATTGGT GATGAAATTA AAGCTGCCGA AGTTGCTTTA TCAGAGCTAC AAAACGAGAT TGAGCAAAAA GCGCTTAGCA TTCCTAACTT GCCGGACGAA TCTGTACCAG CTGGTAAAGA TGAAAATGAT AACGTTGAAA TCTCAAAATG GGGTACACCT CGTCAGTTCG ATTTTGAAAT CAAAGATCAT ACTGATTTAG GTGAGTGGAT GGGCGGACTG GAGTTTGAAA CTGCCACTAA ACTCACAGGC TCACGTTTTA GTGTGTTGAA GGGACCTTTA GCGCGTCTAC AACGTGCTTT AACTCAGTTC ATGCTCGATA CCCATACTTT AAAGAATGGT TATACCGAAG CGTATGTGCC TTATCTGGTA AATGCTGATT CATTACGTGG GACAGGTCAG TTACCAAAAT TTGAAGAAGA TTTATTTAAA CTTCAAGGCG AAAAAGAATA CTACCTCATC CCTACAGCAG AAGTTCCAGT CACTAACTTT GTCCGTGATG AAATTATTGA TGCAGACCGT TTACCATTAA AATATGCGGC TCATACACCA TGTTTCCGTA GTGAAGCGGG TTCTTATGGT CGTGATACAC GTGGTTTAAT TCGTCAGCAC CAATTTGATA AAGTTGAAAT GGTGCAAATC GTTAAGCCTG AAACATCTAT GCAGGCACTT GAAGAGCTTA CTGCACATGC TGAGGGCATT TTACAGGCGC TTGGTTTGCC TTACCGCAAA ATTTTGCTCT GTGGTGGTGA TATGGGCTTC GGTGCGACTA AAACTTATGA TTTAGAAGTT TGGGTTCCAA GTCAAAATAC TTACCGTGAA ATTTCAAGCT GCTCTAATAT GGGTGATTTC CAGGCACGTC GTATGAAAGC ACGCTACCGT ATGGATCAGA AGAAGACCGA ATTGGTGCAC ACTCTAAATG GTTCAGGCCT TGCTGTAGGG CGTACTTTAC TTGCAGTGAT GGAAAACTAC CAACGCGAAG ACGGTTCAAT TGAGATTCCA GAAGTATTAC GCCCATATAT GGGTGGTGCA ACGTTTATTG ATTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT