AcbaC.01152.a
uroporphyrinogen decarboxylase
| CENTER ID: | AcbaC.01152.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01152.a.B1.GE38681 | full length | 1 | 357 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01152.a.B1.PW37671 | full length | 1 | 357 |
Structures
4ZR8
DEPOSITED: 5/11/2015
DETERMINATION: XRay
CLONE: AcbaC.01152.a.B1.GE38681
PROTEIN: AcbaC.01152.a.B1.PW37671
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30941.1 |
| PATRIC ID: | fig|470.1345.peg.1154 |
| PGAT: | acinetobacter:ABUW_1190 |
| RefSeq: | WP_000209397.1 |
| UniProt: | V5VBG6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTLKNDRFL RALLREPVDT TPIWMMRQAG RYLPEYRETR SKAGDFLSLC KNTEFACEVT LQPLRRYDLD AAILFSDILT IPDALGLGLY FETGEGPKFH KTVRTEQDVA NLPKLNAKAD LDYVMNAVST IRSALGGQVP LIGFSGSPWT LATYMVEGGS SKEFRFTKQM MYAQPEVLHA LLDHLADSVI DYLNAQIDAG AQAIQIFDSW GGALAHREYV EFSLNYMKKI IAGLQREKDG RRIPVIVFTK GGGQWLEPMI TTGADALGLD WTTPLNTART TVAGRVALQG NLDPAVLYGS AASIEKAVKA MLDDAYANGE KTGYVANLGH GITQWVDPAQ PKIFVDTVHE YSAKYLG
NT Sequence
ATGACGACAC TTAAAAATGA TCGTTTCTTA CGTGCTTTAT TACGTGAGCC TGTAGATACC ACTCCAATAT GGATGATGCG CCAAGCAGGA CGTTATTTGC CTGAATATCG GGAAACACGC TCAAAAGCTG GTGACTTTCT ATCTTTATGC AAAAATACAG AATTTGCTTG TGAAGTGACT TTACAGCCTT TGCGTCGCTA CGATTTAGAC GCAGCAATTT TATTTTCAGA TATTTTAACT ATTCCAGACG CATTAGGACT TGGACTGTAT TTTGAAACTG GAGAAGGTCC GAAGTTTCAT AAAACGGTAC GTACTGAACA AGATGTGGCT AACTTACCTA AGCTTAATGC TAAAGCCGAT CTTGATTATG TCATGAACGC AGTAAGTACG ATTCGTTCTG CTTTAGGTGG ACAAGTTCCA CTGATTGGTT TTTCGGGTAG TCCTTGGACA CTGGCGACTT ATATGGTTGA AGGCGGTTCA AGCAAAGAGT TCCGTTTTAC CAAGCAAATG ATGTATGCGC AGCCAGAAGT TTTGCATGCG TTGCTCGACC ATTTAGCAGA TTCAGTCATT GATTACTTAA ATGCGCAAAT TGATGCAGGC GCTCAGGCAA TTCAAATCTT TGATAGTTGG GGTGGGGCTT TAGCACACCG TGAATATGTA GAGTTCTCTC TTAACTATAT GAAGAAGATT ATCGCGGGCT TACAACGTGA AAAAGATGGA CGCCGTATTC CAGTGATTGT GTTCACTAAA GGTGGCGGTC AATGGTTAGA ACCAATGATC ACGACAGGTG CCGATGCATT AGGGCTGGAC TGGACGACAC CATTAAATAC GGCTCGAACC ACGGTGGCAG GTCGTGTTGC CCTACAAGGG AACCTTGACC CGGCTGTTTT ATATGGTTCG GCGGCTTCAA TTGAAAAAGC TGTAAAAGCA ATGTTAGATG ATGCTTATGC AAACGGTGAG AAGACCGGTT ATGTTGCTAA CTTAGGGCAC GGTATTACCC AATGGGTAGA TCCGGCGCAG CCAAAAATCT TTGTAGATAC GGTACATGAA TATAGTGCTA AATATCTAGG ATAG
Details for AcbaC.01152.a.B1.GE38681
| HARVESTED ON: | 12/9/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMT TLKNDRFLRA LLREPVDTTP IWMMRQAGRY LPEYRETRSK AGDFLSLCKN TEFACEVTLQ PLRRYDLDAA ILFSDILTIP DALGLGLYFE TGEGPKFHKT VRTEQDVANL PKLNAKADLD YVMNAVSTIR SALGGQVPLI GFSGSPWTLA TYMVEGGSSK EFRFTKQMMY AQPEVLHALL DHLADSVIDY LNAQIDAGAQ AIQIFDSWGG ALAHREYVEF SLNYMKKIIA GLQREKDGRR IPVIVFTKGG GQWLEPMITT GADALGLDWT TPLNTARTTV AGRVALQGNL DPAVLYGSAA SIEKAVKAML DDAYANGEKT GYVANLGHGI TQWVDPAQPK IFVDTVH
Validated NT Sequence
tcatgtaccg tatctacaaa gatttttggc tgcgccggat ctacccattg ggtaataccg tgccctaagt tagcaacata accggtcttc tcaccgtttg cataagcatc atctaacatt gcttttacag ctttttcaat tgaagccgcc gaaccatata aaacagccgg gtcaaggttc ccttgtaggg caacacgacc tgccaccgtg gttcgagccg tatttaatgg tgtcgtccag tccagcccta atgcatcggc acctgtcgtg atcattggtt ctaaccattg accgccacct ttagtgaaca caatcactgg aatacggcgt ccatcttttt cacgttgtaa gcccgcgata atcttcttca tatagttaag agagaactct acatattcac ggtgtgctaa agccccaccc caactatcaa agatttgaat tgcctgagcg cctgcatcaa tttgcgcatt taagtaatca atgactgaat ctgctaaatg gtcgagcaac gcatgcaaaa cttctggctg cgcatacatc atttgcttgg taaaacggaa ctctttgctt gaaccgcctt caaccatata agtcgccagt gtccaaggac tacccgaaaa accaatcagt ggaacttgtc cacctaaagc agaacgaatc gtacttactg cgttcatgac ataatcaaga tcggctttag cattaagctt aggtaagtta gccacatctt gttcagtacg taccgtttta tgaaacttcg gaccttctcc agtttcaaaa tacagtccaa gtcctaatgc gtctggaata gttaaaatat ctgaaaataa aattgctgcg tctaaatcgt agcgacgcaa aggctgtaaa gtcacttcac aagcaaattc tgtatttttg cataaagata gaaagtcacc agcttttgag cgtgtttccc gatattcagg caaataacgt cctgcttggc gcatcatcca tattggagtg gtatctacag gctcacgtaa taaagcacgt aagaaacgat catttttaag tgtcgtcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT TLKNDRFLRA LLREPVDTTP IWMMRQAGRY LPEYRETRSK AGDFLSLCKN TEFACEVTLQ PLRRYDLDAA ILFSDILTIP DALGLGLYFE TGEGPKFHKT VRTEQDVANL PKLNAKADLD YVMNAVSTIR SALGGQVPLI GFSGSPWTLA TYMVEGGSSK EFRFTKQMMY AQPEVLHALL DHLADSVIDY LNAQIDAGAQ AIQIFDSWGG ALAHREYVEF SLNYMKKIIA GLQREKDGRR IPVIVFTKGG GQWLEPMITT GADALGLDWT TPLNTARTTV AGRVALQGNL DPAVLYGSAA SIEKAVKAML DDAYANGEKT GYVANLGHGI TQWVDPAQPK IFVDTVHEYS AKYLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cacttaaaaa tgatcgtttc ttacgtgctt tattacgtga gcctgtagat accactccaa tatggatgat gcgccaagca ggacgttatt tgcctgaata tcgggaaaca cgctcaaaag ctggtgactt tctatcttta tgcaaaaata cagaatttgc ttgtgaagtg actttacagc ctttgcgtcg ctacgattta gacgcagcaa ttttattttc agatatttta actattccag acgcattagg acttggactg tattttgaaa ctggagaagg tccgaagttt cataaaacgg tacgtactga acaagatgtg gctaacttac ctaagcttaa tgctaaagcc gatcttgatt atgtcatgaa cgcagtaagt acgattcgtt ctgctttagg tggacaagtt ccactgattg gtttttcggg tagtccttgg acactggcga cttatatggt tgaaggcggt tcaagcaaag agttccgttt taccaagcaa atgatgtatg cgcagccaga agttttgcat gcgttgctcg accatttagc agattcagtc attgattact taaatgcgca aattgatgca ggcgctcagg caattcaaat ctttgatagt tggggtgggg ctttagcaca ccgtgaatat gtagagttct ctcttaacta tatgaagaag attatcgcgg gcttacaacg tgaaaaagat ggacgccgta ttccagtgat tgtgttcact aaaggtggcg gtcaatggtt agaaccaatg atcacgacag gtgccgatgc attagggctg gactggacga caccattaaa tacggctcga accacggtgg caggtcgtgt tgccctacaa gggaaccttg acccggctgt tttatatggt tcggcggctt caattgaaaa agctgtaaaa gcaatgttag atgatgctta tgcaaacggt gagaagaccg gttatgttgc taacttaggg cacggtatta cccaatgggt agatccggcg cagccaaaaa tctttgtaga tacggtacat gaatatagtg ctaaatatct aggatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.01152.a.B1.PW37671
| PURIFICATION DATe: | 3/10/2015 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 20 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT TLKNDRFLRA LLREPVDTTP IWMMRQAGRY LPEYRETRSK AGDFLSLCKN TEFACEVTLQ PLRRYDLDAA ILFSDILTIP DALGLGLYFE TGEGPKFHKT VRTEQDVANL PKLNAKADLD YVMNAVSTIR SALGGQVPLI GFSGSPWTLA TYMVEGGSSK EFRFTKQMMY AQPEVLHALL DHLADSVIDY LNAQIDAGAQ AIQIFDSWGG ALAHREYVEF SLNYMKKIIA GLQREKDGRR IPVIVFTKGG GQWLEPMITT GADALGLDWT TPLNTARTTV AGRVALQGNL DPAVLYGSAA SIEKAVKAML DDAYANGEKT GYVANLGHGI TQWVDPAQPK IFVDTVH
Validated NT Sequence
tcatgtaccg tatctacaaa gatttttggc tgcgccggat ctacccattg ggtaataccg tgccctaagt tagcaacata accggtcttc tcaccgtttg cataagcatc atctaacatt gcttttacag ctttttcaat tgaagccgcc gaaccatata aaacagccgg gtcaaggttc ccttgtaggg caacacgacc tgccaccgtg gttcgagccg tatttaatgg tgtcgtccag tccagcccta atgcatcggc acctgtcgtg atcattggtt ctaaccattg accgccacct ttagtgaaca caatcactgg aatacggcgt ccatcttttt cacgttgtaa gcccgcgata atcttcttca tatagttaag agagaactct acatattcac ggtgtgctaa agccccaccc caactatcaa agatttgaat tgcctgagcg cctgcatcaa tttgcgcatt taagtaatca atgactgaat ctgctaaatg gtcgagcaac gcatgcaaaa cttctggctg cgcatacatc atttgcttgg taaaacggaa ctctttgctt gaaccgcctt caaccatata agtcgccagt gtccaaggac tacccgaaaa accaatcagt ggaacttgtc cacctaaagc agaacgaatc gtacttactg cgttcatgac ataatcaaga tcggctttag cattaagctt aggtaagtta gccacatctt gttcagtacg taccgtttta tgaaacttcg gaccttctcc agtttcaaaa tacagtccaa gtcctaatgc gtctggaata gttaaaatat ctgaaaataa aattgctgcg tctaaatcgt agcgacgcaa aggctgtaaa gtcacttcac aagcaaattc tgtatttttg cataaagata gaaagtcacc agcttttgag cgtgtttccc gatattcagg caaataacgt cctgcttggc gcatcatcca tattggagtg gtatctacag gctcacgtaa taaagcacgt aagaaacgat catttttaag tgtcgtcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMT TLKNDRFLRA LLREPVDTTP IWMMRQAGRY LPEYRETRSK AGDFLSLCKN TEFACEVTLQ PLRRYDLDAA ILFSDILTIP DALGLGLYFE TGEGPKFHKT VRTEQDVANL PKLNAKADLD YVMNAVSTIR SALGGQVPLI GFSGSPWTLA TYMVEGGSSK EFRFTKQMMY AQPEVLHALL DHLADSVIDY LNAQIDAGAQ AIQIFDSWGG ALAHREYVEF SLNYMKKIIA GLQREKDGRR IPVIVFTKGG GQWLEPMITT GADALGLDWT TPLNTARTTV AGRVALQGNL DPAVLYGSAA SIEKAVKAML DDAYANGEKT GYVANLGHGI TQWVDPAQPK IFVDTVHEYS AKYLG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA CACTTAAAAA TGATCGTTTC TTACGTGCTT TATTACGTGA GCCTGTAGAT ACCACTCCAA TATGGATGAT GCGCCAAGCA GGACGTTATT TGCCTGAATA TCGGGAAACA CGCTCAAAAG CTGGTGACTT TCTATCTTTA TGCAAAAATA CAGAATTTGC TTGTGAAGTG ACTTTACAGC CTTTGCGTCG CTACGATTTA GACGCAGCAA TTTTATTTTC AGATATTTTA ACTATTCCAG ACGCATTAGG ACTTGGACTG TATTTTGAAA CTGGAGAAGG TCCGAAGTTT CATAAAACGG TACGTACTGA ACAAGATGTG GCTAACTTAC CTAAGCTTAA TGCTAAAGCC GATCTTGATT ATGTCATGAA CGCAGTAAGT ACGATTCGTT CTGCTTTAGG TGGACAAGTT CCACTGATTG GTTTTTCGGG TAGTCCTTGG ACACTGGCGA CTTATATGGT TGAAGGCGGT TCAAGCAAAG AGTTCCGTTT TACCAAGCAA ATGATGTATG CGCAGCCAGA AGTTTTGCAT GCGTTGCTCG ACCATTTAGC AGATTCAGTC ATTGATTACT TAAATGCGCA AATTGATGCA GGCGCTCAGG CAATTCAAAT CTTTGATAGT TGGGGTGGGG CTTTAGCACA CCGTGAATAT GTAGAGTTCT CTCTTAACTA TATGAAGAAG ATTATCGCGG GCTTACAACG TGAAAAAGAT GGACGCCGTA TTCCAGTGAT TGTGTTCACT AAAGGTGGCG GTCAATGGTT AGAACCAATG ATCACGACAG GTGCCGATGC ATTAGGGCTG GACTGGACGA CACCATTAAA TACGGCTCGA ACCACGGTGG CAGGTCGTGT TGCCCTACAA GGGAACCTTG ACCCGGCTGT TTTATATGGT TCGGCGGCTT CAATTGAAAA AGCTGTAAAA GCAATGTTAG ATGATGCTTA TGCAAACGGT GAGAAGACCG GTTATGTTGC TAACTTAGGG CACGGTATTA CCCAATGGGT AGATCCGGCG CAGCCAAAAA TCTTTGTAGA TACGGTACAT GAATATAGTG CTAAATATCT AGGATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT