SSGCID: Target Page

AcbaC.01142.a

ribosomal protein L3

CENTER ID:	AcbaC.01142.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.01142.a.B1.GE38679	full length		1	212

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.392
GenBank:	AKA30192.1
PGAT:	acinetobacter:ABUW_0406
RefSeq:	WP_001982642.1
UniProt:	V5V9M8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIGLVGRKC GMTRIFTDAG VSVPVTVIEV DPNRITQIKT LETDGYQAVQ VTTGERRESR VTNAQKGHFA KAGVAAGRLV KEFRVTEAEL EGREVGGTIG VDLFTVGQIV DVTGQSKGKG FQGGVKRWNF RTQDATHGNS VSHRVLGSTG QNQTPGRVFK GKKMAGHLGD ERVTVQGLEI VSVDTERSVL VVKGAIPGAT GGDVIVRPTI KA

NT Sequence

ATGGCTATTG GTTTAGTCGG TCGCAAATGT GGTATGACTC GCATCTTTAC AGATGCTGGT GTTTCTGTAC CTGTTACAGT CATCGAAGTC GATCCAAACC GCATTACGCA AATCAAAACA CTTGAAACTG ATGGTTATCA AGCTGTTCAA GTAACTACTG GTGAACGTCG CGAGTCTCGC GTAACTAACG CTCAAAAAGG TCACTTCGCT AAAGCGGGTG TTGCTGCTGG TCGTTTAGTT AAAGAGTTTC GTGTTACTGA AGCTGAGCTT GAAGGCCGTG AAGTTGGCGG TACTATTGGC GTTGATTTGT TCACAGTTGG TCAAATTGTT GACGTAACTG GTCAATCAAA AGGTAAAGGT TTCCAAGGTG GTGTTAAACG TTGGAATTTC CGTACCCAAG ATGCTACTCA CGGTAACTCT GTTTCTCACC GTGTTTTAGG TTCTACAGGT CAAAACCAAA CTCCTGGACG CGTGTTCAAA GGCAAAAAAA TGGCTGGTCA CTTAGGTGAT GAACGCGTAA CAGTACAAGG TCTTGAAATC GTATCTGTTG ACACTGAACG TTCAGTTTTG GTTGTTAAGG GTGCAATTCC TGGTGCAACT GGCGGTGACG TTATCGTACG TCCTACCATC AAGGCCTGA

Details for AcbaC.01142.a.B1.GE38679

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/10/2014
EXPECTED MW:	23kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA IGLVGRKCGM TRIFTDAGVS VPVTVIEVDP NRITQIKTLE TDGYQAVQVT TGERRESRVT NAQKGHFAKA GVAAGRLVKE FRVTEAELEG REVGGTIGVD LFTVGQIVDV TGQSKGKGFQ GGVKRWNFRT QDATHGNSVS HRVLGSTGQN QTPGRVFKGK KMAGHLGDER VTVQGLEIVS VDTERSVLVV KGAIPGATGG DVIVRPTIKA

Validated NT Sequence

atcttactca cttaggcctt gatggtagga cgtacgataa cgtcaccgcc agttgcacca ggaattgcac ccttaacaac caaaactgaa cgttcagtgt caacagatac gatttcaaga ccttgtactg ttacgcgttc atcacctaag tgaccagcca tttttttgcc tttgaacacg cgtccaggag tttggttttg acctgtagaa cctaaaacac ggtgagaaac agagttaccg tgagtagcat cttgggtacg gaaattccaa cgtttaacac caccttggaa acctttacct tttgattgac cagttacgtc aacaatttga ccaactgtga acaaatcaac gccaatagta ccgccaactt cacggccttc aagctcagct tcagtaacac gaaactcttt aactaaacga ccagcagcaa cacccgcttt agcgaagtga cctttttgag cgttagttac gcgagactcg cgacgttcac cagtagttac ttgaacagct tgataaccat cagtttcaag tgttttgatt tgcgtaatgc ggtttggatc gacttcgatg actgtaacag gtacagaaac accagcatct gtaaagatgc gagtcatacc acatttgcga ccgactaaac caatagccat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta ttggtttagt cggtcgcaaa tgtggtatga ctcgcatctt tacagatgct ggtgtttctg tacctgttac agtcatcgaa gtcgatccaa accgcattac gcaaatcaaa acacttgaaa ctgatggtta tcaagctgtt caagtaacta ctggtgaacg tcgcgagtct cgcgtaacta acgctcaaaa aggtcacttc gctaaagcgg gtgttgctgc tggtcgttta gttaaagagt ttcgtgttac tgaagctgag cttgaaggcc gtgaagttgg cggtactatt ggcgttgatt tgttcacagt tggtcaaatt gttgacgtaa ctggtcaatc aaaaggtaaa ggtttccaag gtggtgttaa acgttggaat ttccgtaccc aagatgctac tcacggtaac tctgtttctc accgtgtttt aggttctaca ggtcaaaacc aaactcctgg acgcgtgttc aaaggcaaaa aaatggctgg tcacttaggt gatgaacgcg taacagtaca aggtcttgaa atcgtatctg ttgacactga acgttcagtt ttggttgtta agggtgcaat tcctggtgca actggcggtg acgttatcgt acgtcctacc atcaaggcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat