AcbaC.01136.a
1-deoxy-D-xylulose 5-phosphate reductoisomerase
| CENTER ID: | AcbaC.01136.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01136.a.B1.GE38678 | full length | 1 | 398 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4ZN6
DEPOSITED: 5/4/2015
DETERMINATION: XRay
CLONE: AcbaC.01136.a.B1.GE38678
PROTEIN: AcbaC.01136.a.B1.PS02362
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA31475.1 |
| PATRIC ID: | fig|470.1345.peg.1695 |
| PGAT: | acinetobacter:ABUW_1739 |
| RefSeq: | WP_000194643.1 |
| UniProt: | A0A0D5YGN4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQSVCILGV TGSIGRSTLK ILGQHPDKYS VFAVSAHSRI SELVEICKQF RPKVVVVPEQ KIAELKTLFA QQNISDIDVL AGQEGLVDIA SHTDVDIVMA AIVGAAGLLP TLAAVKAGKR VLLANKEALV MSGEIMMQAA RDHQALLLPV DSEHNAIFQS LPHNYLQADR TGQPQLGVSK ILLTASGGPF LNHSLEQLVH VTPQQACKHP NWSMGQKISV DSATLMNKGL ELIEACHLFS ISEHFVTVVV HPQSIIHSMV QYVDGSTLAQ MGNPDMCTPI AHALAWPERL QTNVPALDLF EYSQLNFQAP DTQKFPALNL ARQAMRAGGL APTILNAANE IAVEAFLMER IGFTSIPQVV EHTLEKLENA AAESIECILD KDKVARSVAQ QYISSIGG
NT Sequence
ATGACACAAT CTGTTTGCAT ATTGGGTGTA ACCGGTTCTA TTGGACGAAG TACCTTAAAA ATTTTAGGTC AACATCCAGA TAAGTATTCA GTCTTTGCAG TTTCGGCGCA TAGTCGAATT TCTGAACTTG TTGAAATCTG CAAACAGTTT CGACCTAAAG TTGTTGTTGT TCCTGAACAG AAAATAGCTG AACTAAAAAC ACTATTTGCT CAGCAAAATA TATCGGATAT TGATGTTTTA GCTGGGCAAG AGGGTTTAGT CGATATCGCA TCTCATACAG ATGTGGATAT TGTTATGGCT GCAATTGTAG GGGCAGCTGG GCTGTTGCCA ACTTTAGCGG CTGTTAAGGC AGGTAAAAGA GTACTACTTG CCAACAAAGA AGCATTAGTT ATGTCTGGTG AAATCATGAT GCAAGCTGCT CGTGATCATC AGGCATTGTT ATTGCCAGTT GATTCGGAAC ATAATGCAAT TTTCCAATCA TTGCCTCATA ATTATTTACA AGCAGATAGA ACAGGGCAAC CACAGCTAGG TGTATCTAAA ATATTGCTTA CAGCATCTGG TGGTCCTTTT TTAAATCATT CCCTAGAACA ATTGGTTCAT GTAACACCGC AACAAGCATG TAAACATCCA AACTGGTCAA TGGGACAAAA AATTTCGGTT GACTCGGCTA CTCTTATGAA TAAAGGGTTA GAGTTAATTG AGGCTTGTCA TTTATTTTCA ATATCTGAAC ATTTTGTTAC AGTTGTTGTT CATCCACAAA GTATCATTCA CTCTATGGTG CAGTATGTTG ATGGCTCAAC ATTAGCACAA ATGGGTAACC CGGATATGTG TACTCCGATT GCACATGCAT TGGCATGGCC TGAGCGTTTA CAAACTAATG TTCCTGCTTT AGATTTATTT GAGTATTCTC AACTTAATTT TCAGGCACCG GATACCCAAA AATTTCCTGC ATTAAATTTG GCTAGACAAG CAATGCGTGC GGGAGGTTTG GCACCAACCA TTTTGAATGC AGCTAATGAA ATTGCAGTTG AAGCATTCTT AATGGAAAGG ATTGGATTTA CAAGTATTCC GCAAGTGGTA GAGCATACTT TGGAAAAATT AGAGAATGCA GCGGCTGAAA GCATCGAGTG CATTTTAGAT AAAGATAAAG TGGCCCGATC TGTAGCTCAG CAATATATAT CAAGTATTGG AGGCTGA
Details for AcbaC.01136.a.B1.GE38678
| HARVESTED ON: | 12/9/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT QSVCILGVTG SIGRSTLKIL GQHPDKYSVF AVSAHSRISE LVEICKQFRP KVVVVPEQKI AELKTLFAQQ NISDIDVLAG QEGLVDIASH TDVDIVMAAI VGAAGLLPTL AAVKAGKRVL LANKEALVMS GEIMMQAARD HQALLLPVDS EHNAIFQSLP HNYLQADRTG QPQLGVSKIL LTASGGPFLN HSLEQLVHVT PQQACKHPNW SMGQKISVDS ATLMNKGLEL IEACHLFSIS EHFVTVVVHP QSIIHSMVQY VDGSTLAQMG NPDMCTPIAH ALAWPERLQT NVPALDLFEY SQLNFQAPDT QKFPALNLAR QAMRAGGLAP TILNAANEIA VEAFLMERIG FTSIPQVVEH TLEKLENAAA ESIECILDKD KVARSVAQQY ISSIGG
Validated NT Sequence
atcttactca cttagcctcc aatacttgat atatattgct gagctacaga tcgggccact ttatctttat ctaaaatgca ctcgatgctt tcagccgctg cattctctaa tttttccaaa gtatgctcta ccacttgcgg aatacttgta aatccaatcc tttccattaa gaatgcttca actgcaattt cattagctgc attcaaaatg gttggtgcca aacctcccgc acgcattgct tgtctagcca aatttaatgc aggaaatttt tgggtatccg gtgcctgaaa attaagttga gaatactcaa ataaatctaa agcaggaaca ttagtttgta aacgctcagg ccatgccaat gcatgtgcaa tcggagtaca catatccggg ttacccattt gtgctaatgt tgagccatca acatactgca ccatagagtg aatgatactt tgtggatgaa caacaactgt aacaaaatgt tcagatattg aaaataaatg acaagcctca attaactcta accctttatt cataagagta gccgagtcaa ccgaaatttt ttgtcccatt gaccagtttg gatgtttaca tgcttgttgc ggtgttacat gaaccaattg ttctagggaa tgatttaaaa aaggaccacc agatgctgta agcaatattt tagatacacc tagctgtggt tgccctgttc tatctgcttg taaataatta tgaggcaatg attggaaaat tgcattatgt tccgaatcaa ctggcaataa caatgcctga tgatcacgag cagcttgcat catgatttca ccagacataa ctaatgcttc tttgttggca agtagtactc ttttacctgc cttaacagcc gctaaagttg gcaacagccc agctgcccct acaattgcag ccataacaat atccacatct gtatgagatg cgatatcgac taaaccctct tgcccagcta aaacatcaat atccgatata ttttgctgag caaatagtgt ttttagttca gctattttct gttcaggaac aacaacaact ttaggtcgaa actgtttgca gatttcaaca agttcagaaa ttcgactatg cgccgaaact gcaaagactg aatacttatc tggatgttga cctaaaattt ttaaggtact tcgtccaata gaaccggtta cacccaatat gcaaacagat tgtgtcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMT QSVCILGVTG SIGRSTLKIL GQHPDKYSVF AVSAHSRISE LVEICKQFRP KVVVVPEQKI AELKTLFAQQ NISDIDVLAG QEGLVDIASH TDVDIVMAAI VGAAGLLPTL AAVKAGKRVL LANKEALVMS GEIMMQAARD HQALLLPVDS EHNAIFQSLP HNYLQADRTG QPQLGVSKIL LTASGGPFLN HSLEQLVHVT PQQACKHPNW SMGQKISVDS ATLMNKGLEL IEACHLFSIS EHFVTVVVHP QSIIHSMVQY VDGSTLAQMG NPDMCTPIAH ALAWPERLQT NVPALDLFEY SQLNFQAPDT QKFPALNLAR QAMRAGGLAP TILNAANEIA VEAFLMERIG FTSIPQVVEH TLEKLENAAA ESIECILDKD KVARSVAQQY ISSIGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac aatctgtttg catattgggt gtaaccggtt ctattggacg aagtacctta aaaattttag gtcaacatcc agataagtat tcagtctttg cagtttcggc gcatagtcga atttctgaac ttgttgaaat ctgcaaacag tttcgaccta aagttgttgt tgttcctgaa cagaaaatag ctgaactaaa aacactattt gctcagcaaa atatatcgga tattgatgtt ttagctgggc aagagggttt agtcgatatc gcatctcata cagatgtgga tattgttatg gctgcaattg taggggcagc tgggctgttg ccaactttag cggctgttaa ggcaggtaaa agagtactac ttgccaacaa agaagcatta gttatgtctg gtgaaatcat gatgcaagct gctcgtgatc atcaggcatt gttattgcca gttgattcgg aacataatgc aattttccaa tcattgcctc ataattattt acaagcagat agaacagggc aaccacagct aggtgtatct aaaatattgc ttacagcatc tggtggtcct tttttaaatc attccctaga acaattggtt catgtaacac cgcaacaagc atgtaaacat ccaaactggt caatgggaca aaaaatttcg gttgactcgg ctactcttat gaataaaggg ttagagttaa ttgaggcttg tcatttattt tcaatatctg aacattttgt tacagttgtt gttcatccac aaagtatcat tcactctatg gtgcagtatg ttgatggctc aacattagca caaatgggta acccggatat gtgtactccg attgcacatg cattggcatg gcctgagcgt ttacaaacta atgttcctgc tttagattta tttgagtatt ctcaacttaa ttttcaggca ccggataccc aaaaatttcc tgcattaaat ttggctagac aagcaatgcg tgcgggaggt ttggcaccaa ccattttgaa tgcagctaat gaaattgcag ttgaagcatt cttaatggaa aggattggat ttacaagtat tccgcaagtg gtagagcata ctttggaaaa attagagaat gcagcggctg aaagcatcga gtgcatttta gataaagata aagtggcccg atctgtagct cagcaatata tatcaagtat tggaggctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at