AcbaC.01066.a

5-enolpyruvylshikimate-3-phosphate synthase AroA

CENTER ID: AcbaC.01066.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.01066.a.B2.GE38677 mature protein 31 756
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1314.peg.3633
GenBank: AKA31111.1
PGAT: acinetobacter:ABUW_1366
UniProt: A0A090C137

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
LSKSKVEVMS QPLFKKVAFI GLGLIGSSLA RVIVAEKLAT TIVASTRSQK TLEDAKSLGL IQEGFSDPVE AVKGADLVVL ALPVRATQKV LEQIKPYLSE TTIVTDVGST KGNVVDAAKA VFGEDLPAGF VPGHPIAGSE HTGVHAGKVD LFANHKVILT PLPTSAEWAV EKLIQLWSAA KAEVICMDVA KHDEVLAHTS HLPHLMAFNL VEQLANREDN LDIFRYAAGG FRDFSRIAAS DPQMWHDIFF ANKTAILNAV DGFEKQLTVL RKLIENEDSH ALMGLLGHAQ AARQHFNHML AKKPLMEKNK VTQQFSILPG NKAFKGKFTV PGDKSVSHRS IMFGAIAEGT THVTGFLEGE DALATLQAFR DMGVSIEGPK NGEVTIHGVG MHGLKAPASA LYMGNSGTSM RLLSGMLSAQ KFDSVMTGDA SLSKRPMERI AKPLRLMGAQ IQTTGEKGTP PVSITGGQQL KGIQYDLPMA SAQVKSGILL AGLWAEGETS VTEPEPTRDH TERMLRAFGY DVKTEGNKIS LVGGGKLVGT NIQVPSDISS AAFFMVGAAI TEGADVVLEA VGINPTRTGV IEILKQMGAD LTVENERIAG GEPIADIHIK GSRTLKGIHM PEDQVPLAID EFPALFIAAA CAEGQTVLTG AAELRVKESD RIQVMADGLK IMGIDCTPTE DGIIIEGKGK SGDWSPIFAG GEIESHHDHR IAMSFSMAGL RTSGPITIHG TETVATSFPT FTELANRAGL TIEVSQ
NT Sequence
TTGAGCAAAA GTAAAGTTGA AGTTATGTCA CAACCCCTAT TTAAAAAAGT TGCATTTATT GGTTTAGGAC TCATTGGGTC GAGTTTGGCT CGCGTTATTG TTGCAGAAAA GTTGGCGACA ACTATTGTGG CATCTACACG TTCACAGAAA ACTTTAGAAG ATGCAAAATC ACTTGGCTTA ATACAAGAAG GGTTTTCCGA TCCTGTAGAA GCTGTGAAAG GGGCGGATTT AGTTGTTTTA GCCTTGCCAG TGCGTGCCAC TCAAAAAGTG CTTGAGCAAA TTAAACCTTA TTTGTCTGAA ACCACAATTG TAACTGACGT GGGAAGTACA AAAGGTAATG TCGTAGATGC AGCAAAAGCT GTATTTGGTG AAGATTTACC AGCAGGGTTT GTTCCCGGGC ATCCGATTGC GGGAAGTGAA CACACGGGTG TCCACGCAGG TAAGGTCGAT CTCTTTGCAA ATCACAAAGT GATTTTGACG CCATTACCAA CCAGTGCAGA ATGGGCAGTT GAGAAACTGA TTCAACTTTG GTCAGCTGCT AAAGCCGAAG TGATATGTAT GGATGTTGCC AAACATGATG AAGTTTTGGC GCATACCAGT CATTTACCGC ACTTAATGGC ATTTAATCTG GTCGAGCAAC TTGCAAACCG TGAAGATAAT CTTGATATTT TCCGTTATGC AGCGGGTGGT TTTCGTGACT TTTCACGAAT TGCTGCCAGT GATCCTCAAA TGTGGCATGA CATTTTCTTT GCCAATAAAA CAGCCATATT AAATGCTGTA GATGGCTTCG AGAAACAACT TACCGTACTT AGAAAATTGA TTGAAAACGA AGATTCTCAT GCATTAATGG GTTTACTCGG CCATGCTCAG GCAGCACGTC AGCATTTCAA TCATATGTTA GCCAAAAAAC CTTTAATGGA GAAAAATAAG GTGACACAGC AATTCAGTAT CTTACCGGGA AATAAGGCTT TTAAAGGTAA ATTTACCGTA CCGGGTGACA AATCTGTGTC ACATCGCTCC ATTATGTTTG GTGCTATTGC AGAAGGCACT ACGCATGTAA CAGGCTTCCT TGAAGGTGAA GATGCTTTAG CAACTTTACA AGCTTTCCGT GATATGGGTG TAAGCATTGA AGGCCCTAAA AATGGTGAAG TGACCATTCA TGGCGTGGGC ATGCATGGCT TAAAAGCACC AGCAAGTGCG TTGTATATGG GTAATTCTGG AACAAGCATG CGTTTGCTTT CAGGTATGCT TTCTGCACAA AAGTTTGATT CTGTTATGAC AGGTGATGCG TCACTTTCTA AACGTCCAAT GGAGCGTATT GCTAAACCAT TACGTTTAAT GGGTGCGCAA ATCCAAACGA CAGGTGAGAA AGGTACACCA CCTGTAAGCA TTACTGGTGG TCAGCAATTA AAAGGTATTC AATACGATTT ACCTATGGCA TCTGCTCAGG TGAAGTCGGG TATTTTACTT GCCGGTTTAT GGGCTGAAGG TGAAACTTCG GTAACTGAAC CGGAACCAAC ACGTGATCAT ACAGAGCGTA TGCTTCGTGC ATTTGGCTAT GATGTTAAAA CTGAAGGCAA TAAAATTTCC CTTGTGGGCG GCGGTAAGTT AGTGGGTACT AATATTCAAG TACCATCTGA TATTTCATCT GCTGCTTTCT TCATGGTGGG CGCTGCAATC ACTGAAGGCG CAGACGTTGT TCTGGAAGCT GTAGGTATTA ATCCAACACG TACTGGCGTA ATTGAAATTT TAAAACAGAT GGGTGCTGAC CTGACTGTTG AAAATGAGCG TATCGCTGGC GGAGAGCCTA TTGCTGATAT TCATATTAAA GGTTCACGTA CCCTTAAAGG CATTCACATG CCTGAAGACC AAGTACCTTT AGCAATTGAT GAATTCCCAG CCTTATTTAT TGCAGCGGCT TGTGCTGAAG GCCAAACAGT ATTAACAGGT GCAGCAGAGC TTCGCGTGAA AGAATCTGAC CGTATTCAAG TTATGGCAGA TGGCTTAAAA ATCATGGGCA TTGATTGCAC ACCTACTGAA GATGGCATCA TTATCGAAGG TAAAGGCAAA TCTGGTGATT GGTCACCAAT CTTTGCTGGT GGTGAAATTG AATCACATCA TGACCACCGT ATTGCCATGA GTTTTAGTAT GGCAGGTCTT CGTACTTCTG GTCCGATTAC AATTCACGGT ACTGAAACTG TTGCTACAAG TTTCCCAACT TTTACTGAGT TGGCGAATCG TGCAGGCTTA ACGATTGAAG TTAGCCAATA A
Details for AcbaC.01066.a.B2.GE38677
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 78kDa
OBSERVED MW: 78kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHRV IVAEKLATTI VASTRSQKTL EDAKSLGLIQ EGFSDPVEAV KGADLVVLAL PVRATQKVLE QIKPYLSETT IVTDVGSTKG NVVDAAKAVF GEDLPAGFVP GHPIAGSEHT GVHAGKVDLF ANHKVILTPL PTSAEWAVEK LIQLWSAAKA EVICMDVAKH DEVLAHTSHL PHLMAFNLVE QLANREDNLD IFRYAAGGFR DFSRIAASDP QMWHDIFFAN KTAILNAVDG FEKQLTVLRK LIENEDSHAL MGLLGHAQAA RQHFNHMLAK KPLMEKNKVT QQFSILPGNK AFKGKFTVPG DKSVSHRSIM FGAIAEGTTH VTGFLEGEDA LATLQAFRDM GVSIEGPKNG EVTIHGVGMH GLKAPASALY MGNSGTSMRL LSGMLSAQKF DSVMTGDASL SKRPMERIAK PLRLMGAQIQ TTGEKGTPPV SITGGQQLKG IQYDLPMASA QVKSGILLAG LWAEGETSVT EPEPTRDHTE RMLRAFGYDV KTEGNKISLV GGGKLVGTNI QVPSDISSAA FFMVGAAITE GADVVLEAVG INPTRTGVIE ILKQMGADLT VENERIAGGE PIADIHIKGS RTLKGIHMPE DQVPLAIDEF PALFIAAACA EGQTVLTGAA ELRVKESDRI QVMADGLKIM GIDCTPTEDG IIIEGKGKSG DWSPIFAGGE IESHHDHRIA MSFSMAGLRT SGPITIHGTE TVATSFPTFT ELANRAGLTI EVSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgtta ttgttgcaga aaagttggcg acaactattg tggcatctac acgttcacag aaaactttag aagatgcaaa atcacttggc ttaatacaag aagggttttc cgatcctgta gaagctgtga aaggggcgga tttagttgtt ttagccttgc cagtgcgtgc cactcaaaaa gtgcttgagc aaattaaacc ttatttgtct gaaaccacaa ttgtaactga cgtgggaagt acaaaaggta atgtcgtaga tgcagcaaaa gctgtatttg gtgaagattt accagcaggg tttgttcccg ggcatccgat tgcgggaagt gaacacacgg gtgtccacgc aggtaaggtc gatctctttg caaatcacaa agtgattttg acgccattac caaccagtgc agaatgggca gttgagaaac tgattcaact ttggtcagct gctaaagccg aagtgatatg tatggatgtt gccaaacatg atgaagtttt ggcgcatacc agtcatttac cgcacttaat ggcatttaat ctggtcgagc aacttgcaaa ccgtgaagat aatcttgata ttttccgtta tgcagcgggt ggttttcgtg acttttcacg aattgctgcc agtgatcctc aaatgtggca tgacattttc tttgccaata aaacagccat attaaatgct gtagatggct tcgagaaaca acttaccgta cttagaaaat tgattgaaaa cgaagattct catgcattaa tgggtttact cggccatgct caggcagcac gtcagcattt caatcatatg ttagccaaaa aacctttaat ggagaaaaat aaggtgacac agcaattcag tatcttaccg ggaaataagg cttttaaagg taaatttacc gtaccgggtg acaaatctgt gtcacatcgc tccattatgt ttggtgctat tgcagaaggc actacgcatg taacaggctt ccttgaaggt gaagatgctt tagcaacttt acaagctttc cgtgatatgg gtgtaagcat tgaaggccct aaaaatggtg aagtgaccat tcatggcgtg ggcatgcatg gcttaaaagc accagcaagt gcgttgtata tgggtaattc tggaacaagc atgcgtttgc tttcaggtat gctttctgca caaaagtttg attctgttat gacaggtgat gcgtcacttt ctaaacgtcc aatggagcgt attgctaaac cattacgttt aatgggtgcg caaatccaaa cgacaggtga gaaaggtaca ccacctgtaa gcattactgg tggtcagcaa ttaaaaggta ttcaatacga tttacctatg gcatctgctc aggtgaagtc gggtatttta cttgccggtt tatgggctga aggtgaaact tcggtaactg aaccggaacc aacacgtgat catacagagc gtatgcttcg tgcatttggc tatgatgtta aaactgaagg caataaaatt tcccttgtgg gcggcggtaa gttagtgggt actaatattc aagtaccatc tgatatttca tctgctgctt tcttcatggt gggcgctgca atcactgaag gcgcagacgt tgttctggaa gctgtaggta ttaatccaac acgtactggc gtaattgaaa ttttaaaaca gatgggtgct gacctgactg ttgaaaatga gcgtatcgct ggcggagagc ctattgctga tattcatatt aaaggttcac gtacccttaa aggcattcac atgcctgaag accaagtacc tttagcaatt gatgaattcc cagccttatt tattgcagcg gcttgtgctg aaggccaaac agtattaaca ggtgcagcag agcttcgcgt gaaagaatct gaccgtattc aagttatggc agatggctta aaaatcatgg gcattgattg cacacctact gaagatggca tcattatcga aggtaaaggc aaatctggtg attggtcacc aatctttgct ggtggtgaaa ttgaatcaca tcatgaccac cgtattgcca tgagttttag tatggcaggt cttcgtactt ctggtccgat tacaattcac ggtactgaaa ctgttgctac aagtttccca acttttactg agttggcgaa tcgtgcaggc ttaacgattg aagttagcca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat