AcbaC.01032.a
tyrosyl-tRNA synthetase
| CENTER ID: | AcbaC.01032.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01032.a.B1.GE38675 | full length | 1 | 410 | ||
| AcbaC.01032.a.B2.GE40446 | truncation1 | 11 | 410 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.01032.a.B1.PW37669 | full length | 1 | 410 | ||
| AcbaC.01032.a.B2.PW38226 | truncation1 | 11 | 410 |
Structures
6BQY
DEPOSITED: 11/29/2017
DETERMINATION: XRay
CLONE: AcbaC.01032.a.B2.GE40446
PROTEIN: AcbaC.01032.a.B2.PW38226
6BQZ
DEPOSITED: 11/29/2017
DETERMINATION: XRay
CLONE: AcbaC.01032.a.B2.GE40446
PROTEIN: AcbaC.01032.a.B2.PW38226
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA29814.1 |
| PATRIC ID: | fig|470.1345.peg.14 |
| PGAT: | acinetobacter:ABUW_0014 |
| UniProt: | V5V8J9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCLGFVMSNF LPAEEQLALI QRGTHEIISE EDLLKKLKEN RPLKIKAGFD PTAPDLHLGH TVLINKLKTF QDLGHEVTFL IGDYTAMIGD PTGKSATRPP LSREQVEANA KTYQEQVFKI LDPNKTKVRF NSEWFNQKSA ADLIQLASQQ TVSRMLERDD FTKRYNNHQP IAIHEFLYPL VQGYDSIALE ADVELGGTDQ TFNLLMGRTL QSRYGQESQV CITVPILEGL DGVNKMSKSL GNYIGVFDAP GAMYQKVLSM PDSLIERYFD LLSFKSLDEI KALLDEIAAG RNPQEVKRIL ALELVERFHD AEAAANAHKS AGNRITEGEV PADTPEVTIS RGEFGGEIFI ATILRVAGLN PNAAAAKDAV ARGAVKVDWN VVDASFSVKE NGTFIIQSGK KAIARVTFTD
NT Sequence
ATGTGTTTAG GTTTTGTGAT GTCAAATTTC TTGCCCGCCG AAGAACAGCT TGCCCTCATC CAACGAGGCA CGCACGAAAT TATTTCAGAA GAAGATTTAC TGAAAAAGCT TAAAGAGAAT CGTCCTCTTA AAATTAAAGC TGGTTTTGAC CCTACAGCTC CAGATTTACA TTTAGGACAT ACGGTTCTTA TTAACAAACT AAAAACGTTC CAAGACTTGG GCCATGAAGT TACCTTCTTA ATTGGTGACT ACACAGCGAT GATTGGTGAC CCAACGGGGA AAAGTGCAAC TCGTCCGCCG TTATCACGCG AACAAGTTGA AGCTAACGCT AAAACTTATC AAGAACAAGT TTTCAAAATT TTAGATCCGA ATAAAACAAA AGTACGTTTT AACTCAGAGT GGTTTAACCA AAAGTCTGCT GCGGATCTTA TTCAGTTGGC AAGCCAGCAA ACAGTATCAC GTATGTTAGA GCGTGATGAC TTCACTAAGC GTTATAACAA CCATCAGCCA ATTGCAATTC ATGAGTTCTT ATATCCTTTA GTTCAAGGCT ATGACTCTAT CGCACTTGAA GCAGACGTTG AGTTAGGTGG TACAGACCAA ACCTTTAACT TACTTATGGG CCGTACATTA CAAAGCCGTT ATGGCCAAGA ATCACAAGTA TGTATCACTG TACCAATTCT AGAAGGTTTA GATGGCGTAA ATAAAATGTC TAAATCTTTA GGTAACTATA TTGGTGTTTT TGATGCGCCA GGTGCAATGT ACCAAAAAGT ATTATCAATG CCTGACTCTT TAATCGAACG TTATTTCGAT TTATTAAGTT TTAAATCTCT AGATGAGATC AAAGCTTTAT TAGATGAAAT CGCGGCTGGT CGTAATCCAC AAGAAGTAAA ACGTATTCTT GCACTCGAGT TGGTTGAACG CTTCCATGAT GCTGAAGCTG CTGCCAATGC TCATAAGAGT GCTGGTAACC GTATTACAGA AGGTGAGGTG CCGGCAGATA CTCCTGAAGT AACTATTTCA CGTGGTGAGT TTGGTGGTGA AATCTTTATT GCCACGATTC TTCGCGTAGC AGGATTAAAT CCAAATGCAG CAGCTGCAAA AGATGCTGTT GCACGCGGTG CAGTAAAAGT AGACTGGAAT GTAGTAGATG CAAGTTTCTC TGTAAAAGAA AACGGGACTT TCATTATTCA GTCTGGGAAA AAAGCAATTG CACGTGTAAC TTTCACTGAC TAA
Details for AcbaC.01032.a.B1.GE38675
| HARVESTED ON: | 12/9/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMC LGFVMSNFLP AEEQLALIQR GTHEIISEED LLKKLKENRP LKIKAGFDPT APDLHLGHTV LINKLKTFQD LGHEVTFLIG DYTAMIGDPT GKSATRPPLS REQVEANAKT YQEQVFKILD PNKTKVRFNS EWFNQKSAAD LIQLASQQTV SRMLERDDFT KRYNNHQPIA IHEFLYPLVQ GYDSIALEAD VELGGTDQTF NLLMGRTLQS RYGQESQVCI TVPILEGLDG VNKMSKSLGN YIGVFDAPGA MYQKVLSMPD SLIERYFDLL SFKSLDEIKA LLDEIAAGRN PQEVKRILAL ELVERFHDAE AAANAHKSAG NRITEGEVPA DTPEVTISRG EFGGEIFIAT ILRVAGLNPN AAAAKDAVAR GAVKVDWNVV DASFSVKENG TFIIQSGKKA
Validated NT Sequence
attgcttttt tcccagactg aataatgaaa gtcccgtttt cttttacaga gaaacttgca tctactacat tccagtctac ttttactgca ccgcgtgcaa cagcatcttt tgcagctgct gcatttggat ttaatcctgc tacgcgaaga atcgtggcaa taaagatttc accaccaaac tcaccacgtg aaatagttac ttcaggagta tctgccggca cctcaccttc tgtaatacgg ttaccagcac tcttatgagc attggcagca gcttcagcat catggaagcg ttcaaccaac tcgagtgcaa gaatacgttt tacttcttgt ggattacgac cagccgcgat ttcatctaat aaagctttga tctcatctag agatttaaaa cttaataaat cgaaataacg ttcgattaaa gagtcaggca ttgataatac tttttggtac attgcacctg gcgcatcaaa aacaccaata tagttaccta aagatttaga cattttattt acgccatcta aaccttctag aattggtaca gtgatacata cttgtgattc ttggccataa cggctttgta atgtacggcc cataagtaag ttaaaggttt ggtctgtacc acctaactca acgtctgctt caagtgcgat agagtcatag ccttgaacta aaggatataa gaactcatga attgcaattg gctgatggtt gttataacgc ttagtgaagt catcacgctc taacatacgt gatactgttt gctggcttgc caactgaata agatccgcag cagacttttg gttaaaccac tctgagttaa aacgtacttt tgttttattc ggatctaaaa ttttgaaaac ttgttcttga taagttttag cgttagcttc aacttgttcg cgtgataacg gcggacgagt tgcacttttc cccgttgggt caccaatcat cgctgtgtag tcaccaatta agaaggtaac ttcatggccc aagtcttgga acgtttttag tttgttaata agaaccgtat gtcctaaatg taaatctgga gctgtagggt caaaaccagc tttaatttta agaggacgat tctctttaag ctttttcagt aaatcttctt ctgaaataat ttcgtgcgtg cctcgttgga tgagggcaag ctgttcttcg gcgggcaaga aatttgacat cacaaaacct aaacacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMC LGFVMSNFLP AEEQLALIQR GTHEIISEED LLKKLKENRP LKIKAGFDPT APDLHLGHTV LINKLKTFQD LGHEVTFLIG DYTAMIGDPT GKSATRPPLS REQVEANAKT YQEQVFKILD PNKTKVRFNS EWFNQKSAAD LIQLASQQTV SRMLERDDFT KRYNNHQPIA IHEFLYPLVQ GYDSIALEAD VELGGTDQTF NLLMGRTLQS RYGQESQVCI TVPILEGLDG VNKMSKSLGN YIGVFDAPGA MYQKVLSMPD SLIERYFDLL SFKSLDEIKA LLDEIAAGRN PQEVKRILAL ELVERFHDAE AAANAHKSAG NRITEGEVPA DTPEVTISRG EFGGEIFIAT ILRVAGLNPN AAAAKDAVAR GAVKVDWNVV DASFSVKENG TFIIQSGKKA IARVTFTD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgtt taggttttgt gatgtcaaat ttcttgcccg ccgaagaaca gcttgccctc atccaacgag gcacgcacga aattatttca gaagaagatt tactgaaaaa gcttaaagag aatcgtcctc ttaaaattaa agctggtttt gaccctacag ctccagattt acatttagga catacggttc ttattaacaa actaaaaacg ttccaagact tgggccatga agttaccttc ttaattggtg actacacagc gatgattggt gacccaacgg ggaaaagtgc aactcgtccg ccgttatcac gcgaacaagt tgaagctaac gctaaaactt atcaagaaca agttttcaaa attttagatc cgaataaaac aaaagtacgt tttaactcag agtggtttaa ccaaaagtct gctgcggatc ttattcagtt ggcaagccag caaacagtat cacgtatgtt agagcgtgat gacttcacta agcgttataa caaccatcag ccaattgcaa ttcatgagtt cttatatcct ttagttcaag gctatgactc tatcgcactt gaagcagacg ttgagttagg tggtacagac caaaccttta acttacttat gggccgtaca ttacaaagcc gttatggcca agaatcacaa gtatgtatca ctgtaccaat tctagaaggt ttagatggcg taaataaaat gtctaaatct ttaggtaact atattggtgt ttttgatgcg ccaggtgcaa tgtaccaaaa agtattatca atgcctgact ctttaatcga acgttatttc gatttattaa gttttaaatc tctagatgag atcaaagctt tattagatga aatcgcggct ggtcgtaatc cacaagaagt aaaacgtatt cttgcactcg agttggttga acgcttccat gatgctgaag ctgctgccaa tgctcataag agtgctggta accgtattac agaaggtgag gtgccggcag atactcctga agtaactatt tcacgtggtg agtttggtgg tgaaatcttt attgccacga ttcttcgcgt agcaggatta aatccaaatg cagcagctgc aaaagatgct gttgcacgcg gtgcagtaaa agtagactgg aatgtagtag atgcaagttt ctctgtaaaa gaaaacggga ctttcattat tcagtctggg aaaaaagcaa ttgcacgtgt aactttcact gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.01032.a.B2.GE40446
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLP AEEQLALIQR GTHEIISEED LLKKLKENRP LKIKAGFDPT APDLHLGHTV LINKLKTFQD LGHEVTFLIG DYTAMIGDPT GKSATRPPLS REQVEANAKT YQEQVFKILD PNKTKVRFNS EWFNQKSAAD LIQLASQQTV SRMLERDDFT KRYNNHQPIA IHEFLYPLVQ GYDSIALEAD VELGGTDQTF NLLMGRTLQS RYGQESQVCI TVPILEGLDG VNKMSKSLGN YIGVFDAPGA MYQKVLSMPD SLIERYFDLL SFKSLDEIKA LLDEIAAGRN PQEVKRILAL ELVERFHDAE AAANAHKSAG NRITEGEVPA DTPEVTISRG EFGGEIFIAT ILRVAGLNPN AAAAKDAVAR GAVKVDWNVV DASFSVKENG TFIIQSGKKA IARVTFTD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcccg ccgaagaaca gcttgccctc atccaacgag gcacgcacga aattatttca gaagaagatt tactgaaaaa gcttaaagag aatcgtcctc ttaaaattaa agctggtttt gaccctacag ctccagattt acatttagga catacggttc ttattaacaa actaaaaacg ttccaagact tgggccatga agttaccttc ttaattggtg actacacagc gatgattggt gacccaacgg ggaaaagtgc aactcgtccg ccgttatcac gcgaacaagt tgaagctaac gctaaaactt atcaagaaca agttttcaaa attttagatc cgaataaaac aaaagtacgt tttaactcag agtggtttaa ccaaaagtct gctgcggatc ttattcagtt ggcaagccag caaacagtat cacgtatgtt agagcgtgat gacttcacta agcgttataa caaccatcag ccaattgcaa ttcatgagtt cttatatcct ttagttcaag gctatgactc tatcgcactt gaagcagacg ttgagttagg tggtacagac caaaccttta acttacttat gggccgtaca ttacaaagcc gttatggcca agaatcacaa gtatgtatca ctgtaccaat tctagaaggt ttagatggcg taaataaaat gtctaaatct ttaggtaact atattggtgt ttttgatgcg ccaggtgcaa tgtaccaaaa agtattatca atgcctgact ctttaatcga acgttatttc gatttattaa gttttaaatc tctagatgag atcaaagctt tattagatga aatcgcggct ggtcgtaatc cacaagaagt aaaacgtatt cttgcactcg agttggttga acgcttccat gatgctgaag ctgctgccaa tgctcataag agtgctggta accgtattac agaaggtgag gtgccggcag atactcctga agtaactatt tcacgtggtg agtttggtgg tgaaatcttt attgccacga ttcttcgcgt agcaggatta aatccaaatg cagcagctgc aaaagatgct gttgcacgcg gtgcagtaaa agtagactgg aatgtagtag atgcaagttt ctctgtaaaa gaaaacggga ctttcattat tcagtctggg aaaaaagcaa ttgcacgtgt aactttcact gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.01032.a.B1.PW37669
| PURIFICATION DATe: | 3/9/2015 |
| CONCENTRATION: | 21mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC LGFVMSNFLP AEEQLALIQR GTHEIISEED LLKKLKENRP LKIKAGFDPT APDLHLGHTV LINKLKTFQD LGHEVTFLIG DYTAMIGDPT GKSATRPPLS REQVEANAKT YQEQVFKILD PNKTKVRFNS EWFNQKSAAD LIQLASQQTV SRMLERDDFT KRYNNHQPIA IHEFLYPLVQ GYDSIALEAD VELGGTDQTF NLLMGRTLQS RYGQESQVCI TVPILEGLDG VNKMSKSLGN YIGVFDAPGA MYQKVLSMPD SLIERYFDLL SFKSLDEIKA LLDEIAAGRN PQEVKRILAL ELVERFHDAE AAANAHKSAG NRITEGEVPA DTPEVTISRG EFGGEIFIAT ILRVAGLNPN AAAAKDAVAR GAVKVDWNVV DASFSVKENG TFIIQSGKKA
Validated NT Sequence
attgcttttt tcccagactg aataatgaaa gtcccgtttt cttttacaga gaaacttgca tctactacat tccagtctac ttttactgca ccgcgtgcaa cagcatcttt tgcagctgct gcatttggat ttaatcctgc tacgcgaaga atcgtggcaa taaagatttc accaccaaac tcaccacgtg aaatagttac ttcaggagta tctgccggca cctcaccttc tgtaatacgg ttaccagcac tcttatgagc attggcagca gcttcagcat catggaagcg ttcaaccaac tcgagtgcaa gaatacgttt tacttcttgt ggattacgac cagccgcgat ttcatctaat aaagctttga tctcatctag agatttaaaa cttaataaat cgaaataacg ttcgattaaa gagtcaggca ttgataatac tttttggtac attgcacctg gcgcatcaaa aacaccaata tagttaccta aagatttaga cattttattt acgccatcta aaccttctag aattggtaca gtgatacata cttgtgattc ttggccataa cggctttgta atgtacggcc cataagtaag ttaaaggttt ggtctgtacc acctaactca acgtctgctt caagtgcgat agagtcatag ccttgaacta aaggatataa gaactcatga attgcaattg gctgatggtt gttataacgc ttagtgaagt catcacgctc taacatacgt gatactgttt gctggcttgc caactgaata agatccgcag cagacttttg gttaaaccac tctgagttaa aacgtacttt tgttttattc ggatctaaaa ttttgaaaac ttgttcttga taagttttag cgttagcttc aacttgttcg cgtgataacg gcggacgagt tgcacttttc cccgttgggt caccaatcat cgctgtgtag tcaccaatta agaaggtaac ttcatggccc aagtcttgga acgtttttag tttgttaata agaaccgtat gtcctaaatg taaatctgga gctgtagggt caaaaccagc tttaatttta agaggacgat tctctttaag ctttttcagt aaatcttctt ctgaaataat ttcgtgcgtg cctcgttgga tgagggcaag ctgttcttcg gcgggcaaga aatttgacat cacaaaacct aaacacatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMC LGFVMSNFLP AEEQLALIQR GTHEIISEED LLKKLKENRP LKIKAGFDPT APDLHLGHTV LINKLKTFQD LGHEVTFLIG DYTAMIGDPT GKSATRPPLS REQVEANAKT YQEQVFKILD PNKTKVRFNS EWFNQKSAAD LIQLASQQTV SRMLERDDFT KRYNNHQPIA IHEFLYPLVQ GYDSIALEAD VELGGTDQTF NLLMGRTLQS RYGQESQVCI TVPILEGLDG VNKMSKSLGN YIGVFDAPGA MYQKVLSMPD SLIERYFDLL SFKSLDEIKA LLDEIAAGRN PQEVKRILAL ELVERFHDAE AAANAHKSAG NRITEGEVPA DTPEVTISRG EFGGEIFIAT ILRVAGLNPN AAAAKDAVAR GAVKVDWNVV DASFSVKENG TFIIQSGKKA IARVTFTD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGTT TAGGTTTTGT GATGTCAAAT TTCTTGCCCG CCGAAGAACA GCTTGCCCTC ATCCAACGAG GCACGCACGA AATTATTTCA GAAGAAGATT TACTGAAAAA GCTTAAAGAG AATCGTCCTC TTAAAATTAA AGCTGGTTTT GACCCTACAG CTCCAGATTT ACATTTAGGA CATACGGTTC TTATTAACAA ACTAAAAACG TTCCAAGACT TGGGCCATGA AGTTACCTTC TTAATTGGTG ACTACACAGC GATGATTGGT GACCCAACGG GGAAAAGTGC AACTCGTCCG CCGTTATCAC GCGAACAAGT TGAAGCTAAC GCTAAAACTT ATCAAGAACA AGTTTTCAAA ATTTTAGATC CGAATAAAAC AAAAGTACGT TTTAACTCAG AGTGGTTTAA CCAAAAGTCT GCTGCGGATC TTATTCAGTT GGCAAGCCAG CAAACAGTAT CACGTATGTT AGAGCGTGAT GACTTCACTA AGCGTTATAA CAACCATCAG CCAATTGCAA TTCATGAGTT CTTATATCCT TTAGTTCAAG GCTATGACTC TATCGCACTT GAAGCAGACG TTGAGTTAGG TGGTACAGAC CAAACCTTTA ACTTACTTAT GGGCCGTACA TTACAAAGCC GTTATGGCCA AGAATCACAA GTATGTATCA CTGTACCAAT TCTAGAAGGT TTAGATGGCG TAAATAAAAT GTCTAAATCT TTAGGTAACT ATATTGGTGT TTTTGATGCG CCAGGTGCAA TGTACCAAAA AGTATTATCA ATGCCTGACT CTTTAATCGA ACGTTATTTC GATTTATTAA GTTTTAAATC TCTAGATGAG ATCAAAGCTT TATTAGATGA AATCGCGGCT GGTCGTAATC CACAAGAAGT AAAACGTATT CTTGCACTCG AGTTGGTTGA ACGCTTCCAT GATGCTGAAG CTGCTGCCAA TGCTCATAAG AGTGCTGGTA ACCGTATTAC AGAAGGTGAG GTGCCGGCAG ATACTCCTGA AGTAACTATT TCACGTGGTG AGTTTGGTGG TGAAATCTTT ATTGCCACGA TTCTTCGCGT AGCAGGATTA AATCCAAATG CAGCAGCTGC AAAAGATGCT GTTGCACGCG GTGCAGTAAA AGTAGACTGG AATGTAGTAG ATGCAAGTTT CTCTGTAAAA GAAAACGGGA CTTTCATTAT TCAGTCTGGG AAAAAAGCAA TTGCACGTGT AACTTTCACT GACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for AcbaC.01032.a.B2.PW38226
| PURIFICATION DATe: | 3/14/2017 |
| CONCENTRATION: | 30mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLP AEEQLALIQR GTHEIISEED LLKKLKENRP LKIKAGFDPT APDLHLGHTV LINKLKTFQD LGHEVTFLIG DYTAMIGDPT GKSATRPPLS REQVEANAKT YQEQVFKILD PNKTKVRFNS EWFNQKSAAD LIQLASQQTV SRMLERDDFT KRYNNHQPIA IHEFLYPLVQ GYDSIALEAD VELGGTDQTF NLLMGRTLQS RYGQESQVCI TVPILEGLDG VNKMSKSLGN YIGVFDAPGA MYQKVLSMPD SLIERYFDLL SFKSLDEIKA LLDEIAAGRN PQEVKRILAL ELVERFHDAE AAANAHKSAG NRITEGEVPA DTPEVTISRG EFGGEIFIAT ILRVAGLNPN AAAAKDAVAR GAVKVDWNVV DASFSVKENG TFIIQSGKKA IARVTFTD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGCCCG CCGAAGAACA GCTTGCCCTC ATCCAACGAG GCACGCACGA AATTATTTCA GAAGAAGATT TACTGAAAAA GCTTAAAGAG AATCGTCCTC TTAAAATTAA AGCTGGTTTT GACCCTACAG CTCCAGATTT ACATTTAGGA CATACGGTTC TTATTAACAA ACTAAAAACG TTCCAAGACT TGGGCCATGA AGTTACCTTC TTAATTGGTG ACTACACAGC GATGATTGGT GACCCAACGG GGAAAAGTGC AACTCGTCCG CCGTTATCAC GCGAACAAGT TGAAGCTAAC GCTAAAACTT ATCAAGAACA AGTTTTCAAA ATTTTAGATC CGAATAAAAC AAAAGTACGT TTTAACTCAG AGTGGTTTAA CCAAAAGTCT GCTGCGGATC TTATTCAGTT GGCAAGCCAG CAAACAGTAT CACGTATGTT AGAGCGTGAT GACTTCACTA AGCGTTATAA CAACCATCAG CCAATTGCAA TTCATGAGTT CTTATATCCT TTAGTTCAAG GCTATGACTC TATCGCACTT GAAGCAGACG TTGAGTTAGG TGGTACAGAC CAAACCTTTA ACTTACTTAT GGGCCGTACA TTACAAAGCC GTTATGGCCA AGAATCACAA GTATGTATCA CTGTACCAAT TCTAGAAGGT TTAGATGGCG TAAATAAAAT GTCTAAATCT TTAGGTAACT ATATTGGTGT TTTTGATGCG CCAGGTGCAA TGTACCAAAA AGTATTATCA ATGCCTGACT CTTTAATCGA ACGTTATTTC GATTTATTAA GTTTTAAATC TCTAGATGAG ATCAAAGCTT TATTAGATGA AATCGCGGCT GGTCGTAATC CACAAGAAGT AAAACGTATT CTTGCACTCG AGTTGGTTGA ACGCTTCCAT GATGCTGAAG CTGCTGCCAA TGCTCATAAG AGTGCTGGTA ACCGTATTAC AGAAGGTGAG GTGCCGGCAG ATACTCCTGA AGTAACTATT TCACGTGGTG AGTTTGGTGG TGAAATCTTT ATTGCCACGA TTCTTCGCGT AGCAGGATTA AATCCAAATG CAGCAGCTGC AAAAGATGCT GTTGCACGCG GTGCAGTAAA AGTAGACTGG AATGTAGTAG ATGCAAGTTT CTCTGTAAAA GAAAACGGGA CTTTCATTAT TCAGTCTGGG AAAAAAGCAA TTGCACGTGT AACTTTCACT GACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT