SSGCID: Target Page

AcbaC.00983.a

signal recognition particle-docking protein FtsY

CENTER ID:	AcbaC.00983.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00983.a.B1.GE38671	full length		1	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1744
GenBank:	AKA31524.1
PGAT:	acinetobacter:ABUW_1789
RefSeq:	WP_001187344.1
UniProt:	A0A0D5YHX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQQQSNMQNK FLIDAEIGDD DVTLPNLPAI DVPIVESPVK QEVKAEEVVE AAPSTETEQP KSGFFGRMKE GLSKTRRNFT DGMVNILIGG KEIDDELLEE VEEQLLVADI GVDATKTIIT NLTERTARGD LIYSHSLYKA LQEELVALLA PRVKPLHIDP NKSPYVILMV GVNGVGKTTT IGKLAKRLQG EGKKVMLAAG DTFRAAATEQ LQIWGERNDI AVVAQGHGAD SASVIFDAFE SARAKGIDVL IADTAGRLHN KSNLMEELKK VKRVMQKIDA TAPHEIMLVV DAGTGQNAIN QVQEFDQAVG LTGITITKLD GTAKGGVLFN IASRTHVPIR FIGVGEKIDD LRPFSAKSFV AALFETEK

NT Sequence

ATGCAACAAC AATCGAATAT GCAAAATAAA TTCTTGATAG ATGCTGAGAT CGGGGATGAC GATGTCACAT TGCCTAATTT ACCTGCTATC GATGTTCCTA TCGTTGAGTC ACCTGTTAAG CAAGAAGTAA AAGCTGAAGA AGTTGTCGAG GCTGCACCGT CTACAGAAAC TGAGCAACCA AAATCTGGTT TCTTTGGGCG AATGAAAGAA GGCTTGAGCA AAACTCGTCG TAATTTCACC GATGGGATGG TTAATATCTT AATTGGTGGG AAAGAAATTG ATGATGAGTT GCTTGAAGAA GTCGAAGAGC AATTATTAGT TGCAGATATT GGTGTTGATG CAACAAAAAC GATTATTACC AACTTAACTG AACGTACTGC TCGTGGTGAT TTGATCTATT CACATTCACT TTATAAAGCC TTGCAAGAAG AGTTAGTTGC TTTGCTTGCT CCACGTGTGA AGCCTTTGCA TATTGACCCT AATAAATCAC CTTATGTGAT TTTAATGGTT GGGGTAAATG GTGTTGGAAA AACAACAACA ATTGGTAAAT TAGCGAAGCG TTTACAAGGT GAAGGCAAAA AAGTGATGTT GGCGGCAGGT GATACATTCC GTGCTGCTGC TACTGAACAA TTGCAAATCT GGGGTGAGCG TAACGATATT GCTGTAGTTG CTCAAGGTCA TGGTGCCGAT AGTGCATCAG TTATTTTTGA TGCTTTTGAA AGTGCGCGAG CTAAAGGTAT TGATGTACTG ATTGCAGATA CTGCGGGCCG TTTGCATAAC AAAAGTAATT TGATGGAAGA GCTTAAGAAA GTTAAGCGTG TTATGCAAAA AATTGATGCA ACAGCGCCTC ATGAAATTAT GTTGGTTGTA GATGCGGGTA CTGGGCAAAA TGCAATCAAC CAAGTACAAG AATTTGACCA GGCTGTCGGT TTAACAGGTA TTACCATTAC TAAGTTGGAT GGTACAGCAA AAGGTGGAGT ATTATTTAAT ATTGCTAGTC GTACTCATGT GCCAATTCGT TTCATTGGTG TGGGTGAAAA AATTGATGAT TTAAGACCAT TCTCGGCGAA ATCGTTTGTT GCAGCACTAT TTGAAACTGA AAAATAA

Details for AcbaC.00983.a.B1.GE38671

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/10/2014
EXPECTED MW:	41kDa
OBSERVED MW:	16kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ QQSNMQNKFL IDAEIGDDDV TLPNLPAIDV PIVESPVKQE VKAEEVVEAA PSTETEQPKS GFFGRMKEGL SKTRRNFTDG MVNILIGGKE IDDELLEEVE EQLLVADIGV DATKTIITNL TERTARGDLI YSHSLYKALQ EELVALLAPR VKPLHIDPNK SPYVILMVGV NGVGKTTTIG KLAKRLQGEG KKVMLAAGDT FRAAATEQLQ IWGERNDIAV VAQGHGADSA SVIFDAFESA RAKGIDVLIA DTAGRLHNKS NLMEELKKVK RVMQKIDATA PHEIMLVVDA GTGQNAINQV QEFDQAVGLT GITITKLDGT AKGGVLFNIA SRTHVPIRFI GVGEKIDDLR PFSAKSFV

Validated NT Sequence

caacaaacga tttcgccgag aatggtctta aatcatcaat tttttcaccc acaccaatga aacgaattgg cacatgagta cgactagcaa tattaaataa tactccacct tttgctgtac catccaactt agtaatggta atacctgtta aaccgacagc ctggtcaaat tcttgtactt ggttgattgc attttgccca gtacccgcat ctacaaccaa cataatttca tgaggcgctg ttgcatcaat tttttgcata acacgcttaa ctttcttaag ctcttccatc aaattacttt tgttatgcaa acggcccgca gtatctgcaa tcagtacatc aataccttta gctcgcgcac tttcaaaagc atcaaaaata actgatgcac tatcggcacc atgaccttga gcaactacag caatatcgtt acgctcaccc cagatttgca attgttcagt agcagcagca cggaatgtat cacctgccgc caacatcact tttttgcctt caccttgtaa acgcttcgct aatttaccaa ttgttgttgt ttttccaaca ccatttaccc caaccattaa aatcacataa ggtgatttat tagggtcaat atgcaaaggc ttcacacgtg gagcaagcaa agcaactaac tcttcttgca aggctttata aagtgaatgt gaatagatca aatcaccacg agcagtacgt tcagttaagt tggtaataat cgtttttgtt gcatcaacac caatatctgc aactaataat tgctcttcga cttcttcaag caactcatca tcaatttctt tcccaccaat taagatatta accatcccat cggtgaaatt acgacgagtt ttgctcaagc cttctttcat tcgcccaaag aaaccagatt ttggttgctc agtttctgta gacggtgcag cctcgacaac ttcttcagct tttacttctt gcttaacagg tgactcaacg ataggaacat cgatagcagg taaattaggc aatgtgacat cgtcatcccc gatctcagca tctatcaaga atttattttg catattcgat tgttgttgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac aacaatcgaa tatgcaaaat aaattcttga tagatgctga gatcggggat gacgatgtca cattgcctaa tttacctgct atcgatgttc ctatcgttga gtcacctgtt aagcaagaag taaaagctga agaagttgtc gaggctgcac cgtctacaga aactgagcaa ccaaaatctg gtttctttgg gcgaatgaaa gaaggcttga gcaaaactcg tcgtaatttc accgatggga tggttaatat cttaattggt gggaaagaaa ttgatgatga gttgcttgaa gaagtcgaag agcaattatt agttgcagat attggtgttg atgcaacaaa aacgattatt accaacttaa ctgaacgtac tgctcgtggt gatttgatct attcacattc actttataaa gccttgcaag aagagttagt tgctttgctt gctccacgtg tgaagccttt gcatattgac cctaataaat caccttatgt gattttaatg gttggggtaa atggtgttgg aaaaacaaca acaattggta aattagcgaa gcgtttacaa ggtgaaggca aaaaagtgat gttggcggca ggtgatacat tccgtgctgc tgctactgaa caattgcaaa tctggggtga gcgtaacgat attgctgtag ttgctcaagg tcatggtgcc gatagtgcat cagttatttt tgatgctttt gaaagtgcgc gagctaaagg tattgatgta ctgattgcag atactgcggg ccgtttgcat aacaaaagta atttgatgga agagcttaag aaagttaagc gtgttatgca aaaaattgat gcaacagcgc ctcatgaaat tatgttggtt gtagatgcgg gtactgggca aaatgcaatc aaccaagtac aagaatttga ccaggctgtc ggtttaacag gtattaccat tactaagttg gatggtacag caaaaggtgg agtattattt aatattgcta gtcgtactca tgtgccaatt cgtttcattg gtgtgggtga aaaaattgat gatttaagac cattctcggc gaaatcgttt gttgcagcac tatttgaaac tgaaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at