AcbaC.00980.a

phosphoserine aminotransferase

CENTER ID:	AcbaC.00980.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00980.a.B1.GE38670	full length		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00980.a.B1.PW37659	full length		1	359

External Resources

RESOURCE	REFERENCE ID
GenBank:	AKA30723.1
PATRIC ID:	fig\|470.1345.peg.934
PGAT:	acinetobacter:ABUW_0969
RefSeq:	WP_001203181.1
UniProt:	V5VC85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRAYNFCAGP AALPTAVLEK AQQELLDWQG KGLSIMEMSH RSADYVAVAE KAEADLRKLM NIPENYKVLF LQGGASLQFS AIPLNLLGKN NKADYIHTGI WSEKALKEAK RYGDINVVEA GIKVDGKFAI SEQSEWNLSD DAAYVHYADN ETIGGLQFAG VPDVKAPLVC DFSSSILSAP LDVSKFGLIY AGAQKNIGPA GLTIVIIRDD LLDQAKAEIP SILKYADQAK NGSMVNTPST YAWYLSGLVF EWLLEQGGVD AIHKVNLEKA QLLYGYIDSS DFYNNPIAIP NRSIMNVPFT LADEALEKQF LKEAEANHLL NLAGHRSVGG MRASIYNAVP LEGVQALIRF MDDFAKRNG

NT Sequence

ATGCGCGCGT ACAATTTCTG TGCTGGTCCT GCTGCATTAC CTACTGCCGT TTTAGAAAAA GCTCAACAAG AATTGTTGGA TTGGCAAGGT AAGGGTCTAT CCATCATGGA AATGAGTCAC CGTAGTGCTG ACTATGTTGC TGTTGCGGAA AAAGCTGAAG CAGATTTGCG TAAGCTCATG AATATTCCTG AAAACTATAA AGTGCTATTT TTACAAGGTG GCGCGTCATT ACAATTTTCA GCAATTCCTT TAAATCTGCT TGGAAAAAAT AACAAAGCAG ATTATATCCA TACTGGAATT TGGTCTGAAA AAGCTTTAAA AGAAGCAAAA CGTTATGGCG ATATTAATGT TGTAGAAGCG GGTATTAAAG TAGACGGTAA GTTCGCAATT TCTGAGCAAA GCGAATGGAA CTTGTCTGAC GATGCAGCTT ATGTCCATTA TGCTGATAAC GAAACAATTG GCGGTTTACA GTTTGCTGGC GTTCCTGATG TTAAAGCACC ACTGGTATGT GACTTCTCAT CAAGCATTTT ATCTGCTCCA CTTGATGTTT CGAAATTTGG TCTCATCTAT GCAGGTGCGC AAAAAAATAT TGGTCCTGCT GGTTTAACAA TTGTTATTAT CCGTGATGAC TTACTGGATC AAGCTAAAGC AGAGATTCCA AGCATTTTAA AATATGCCGA TCAAGCCAAG AATGGTTCTA TGGTAAATAC ACCATCGACT TATGCTTGGT ACTTGTCTGG TTTAGTATTT GAATGGTTAC TAGAGCAGGG TGGGGTAGAT GCTATCCATA AAGTTAACCT TGAAAAAGCT CAGTTACTCT ATGGTTATAT CGATTCAAGC GACTTTTATA ATAACCCGAT TGCCATTCCA AATCGTTCAA TTATGAATGT ACCATTTACT TTGGCAGATG AAGCTTTAGA AAAGCAATTC TTGAAAGAAG CTGAAGCAAA TCACTTATTG AACTTAGCAG GTCACCGCTC AGTAGGTGGT ATGCGTGCAA GTATTTATAA CGCTGTACCA TTAGAAGGCG TTCAGGCGCT TATCCGCTTT ATGGATGATT TTGCTAAACG TAATGGTTAA

Details for AcbaC.00980.a.B1.GE38670

HARVESTED ON:	12/9/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMR AYNFCAGPAA LPTAVLEKAQ QELLDWQGKG LSIMEMSHRS ADYVAVAEKA EADLRKLMNI PENYKVLFLQ GGASLQFSAI PLNLLGKNNK ADYIHTGIWS EKALKEAKRY GDINVVEAGI KVDGKFAISE QSEWNLSDDA AYVHYADNET IGGLQFAGVP DVKAPLVCDF SSSILSAPLD VSKFGLIYAG AQKNIGPAGL TIVIIRDDLL DQAKAEIPSI LKYADQAKNG SMVNTPSTYA WYLSGLVFEW LLEQGGVDAI HKVNLEKAQL LYGYIDSSDF YNNPIAIPNR SIMNVPFTLA DEALEKQFLK EAEANHLLNL AGHRSVGGMR ASIYNAVPLE GVQALIRF

Validated NT Sequence

ataaagcgga taagcgcctg aacgccttct aatggtacag cgttataaat acttgcacgc ataccaccta ctgagcggtg acctgctaag ttcaataagt gatttgcttc agcttctttc aagaattgct tttctaaagc ttcatctgcc aaagtaaatg gtacattcat aattgaacga tttggaatgg caatcgggtt attataaaag tcgcttgaat cgatataacc atagagtaac tgagcttttt caaggttaac tttatggata gcatctaccc caccctgctc tagtaaccat tcaaatacta aaccagacaa gtaccaagca taagtcgatg gtgtatttac catagaacca ttcttggctt gatcggcata ttttaaaatg cttggaatct ctgctttagc ttgatccagt aagtcatcac ggataataac aattgttaaa ccagcaggac caatattttt ttgcgcacct gcatagatga gaccaaattt cgaaacatca agtggagcag ataaaatgct tgatgagaag tcacatacca gtggtgcttt aacatcagga acgccagcaa actgtaaacc gccaattgtt tcgttatcag cataatggac ataagctgca tcgtcagaca agttccattc gctttgctca gaaattgcga acttaccgtc tactttaata cccgcttcta caacattaat atcgccataa cgttttgctt cttttaaagc tttttcagac caaattccag tatggatata atctgctttg ttattttttc caagcagatt taaaggaatt gctgaaaatt gtaatgacgc gccaccttgt aaaaatagca ctttatagtt ttcaggaata ttcatgagct tacgcaaatc tgcttcagct ttttccgcaa cagcaacata gtcagcacta cggtgactca tttccatgat ggatagaccc ttaccttgcc aatccaacaa ttcttgttga gctttttcta aaacggcagt aggtaatgca gcaggaccag cacagaaatt gtacgcgcgc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg cgtacaattt ctgtgctggt cctgctgcat tacctactgc cgttttagaa aaagctcaac aagaattgtt ggattggcaa ggtaagggtc tatccatcat ggaaatgagt caccgtagtg ctgactatgt tgctgttgcg gaaaaagctg aagcagattt gcgtaagctc atgaatattc ctgaaaacta taaagtgcta tttttacaag gtggcgcgtc attacaattt tcagcaattc ctttaaatct gcttggaaaa aataacaaag cagattatat ccatactgga atttggtctg aaaaagcttt aaaagaagca aaacgttatg gcgatattaa tgttgtagaa gcgggtatta aagtagacgg taagttcgca atttctgagc aaagcgaatg gaacttgtct gacgatgcag cttatgtcca ttatgctgat aacgaaacaa ttggcggttt acagtttgct ggcgttcctg atgttaaagc accactggta tgtgacttct catcaagcat tttatctgct ccacttgatg tttcgaaatt tggtctcatc tatgcaggtg cgcaaaaaaa tattggtcct gctggtttaa caattgttat tatccgtgat gacttactgg atcaagctaa agcagagatt ccaagcattt taaaatatgc cgatcaagcc aagaatggtt ctatggtaaa tacaccatcg acttatgctt ggtacttgtc tggtttagta tttgaatggt tactagagca gggtggggta gatgctatcc ataaagttaa ccttgaaaaa gctcagttac tctatggtta tatcgattca agcgactttt ataataaccc gattgccatt ccaaatcgtt caattatgaa tgtaccattt actttggcag atgaagcttt agaaaagcaa ttcttgaaag aagctgaagc aaatcactta ttgaacttag caggtcaccg ctcagtaggt ggtatgcgtg caagtattta taacgctgta ccattagaag gcgttcaggc gcttatccgc tttatggatg attttgctaa acgtaatggt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for AcbaC.00980.a.B1.PW37659

PURIFICATION DATe:	2/25/2015
CONCENTRATION:	26.5mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	16
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCG CGTACAATTT CTGTGCTGGT CCTGCTGCAT TACCTACTGC CGTTTTAGAA AAAGCTCAAC AAGAATTGTT GGATTGGCAA GGTAAGGGTC TATCCATCAT GGAAATGAGT CACCGTAGTG CTGACTATGT TGCTGTTGCG GAAAAAGCTG AAGCAGATTT GCGTAAGCTC ATGAATATTC CTGAAAACTA TAAAGTGCTA TTTTTACAAG GTGGCGCGTC ATTACAATTT TCAGCAATTC CTTTAAATCT GCTTGGAAAA AATAACAAAG CAGATTATAT CCATACTGGA ATTTGGTCTG AAAAAGCTTT AAAAGAAGCA AAACGTTATG GCGATATTAA TGTTGTAGAA GCGGGTATTA AAGTAGACGG TAAGTTCGCA ATTTCTGAGC AAAGCGAATG GAACTTGTCT GACGATGCAG CTTATGTCCA TTATGCTGAT AACGAAACAA TTGGCGGTTT ACAGTTTGCT GGCGTTCCTG ATGTTAAAGC ACCACTGGTA TGTGACTTCT CATCAAGCAT TTTATCTGCT CCACTTGATG TTTCGAAATT TGGTCTCATC TATGCAGGTG CGCAAAAAAA TATTGGTCCT GCTGGTTTAA CAATTGTTAT TATCCGTGAT GACTTACTGG ATCAAGCTAA AGCAGAGATT CCAAGCATTT TAAAATATGC CGATCAAGCC AAGAATGGTT CTATGGTAAA TACACCATCG ACTTATGCTT GGTACTTGTC TGGTTTAGTA TTTGAATGGT TACTAGAGCA GGGTGGGGTA GATGCTATCC ATAAAGTTAA CCTTGAAAAA GCTCAGTTAC TCTATGGTTA TATCGATTCA AGCGACTTTT ATAATAACCC GATTGCCATT CCAAATCGTT CAATTATGAA TGTACCATTT ACTTTGGCAG ATGAAGCTTT AGAAAAGCAA TTCTTGAAAG AAGCTGAAGC AAATCACTTA TTGAACTTAG CAGGTCACCG CTCAGTAGGT GGTATGCGTG CAAGTATTTA TAACGCTGTA CCATTAGAAG GCGTTCAGGC GCTTATCCGC TTTATGGATG ATTTTGCTAA ACGTAATGGT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT