AcbaC.00941.a

chromosomal replication initiator protein DnaA

CENTER ID: AcbaC.00941.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00941.a.B1.GE38667 full length 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1
GenBank: AKA29801.1
PGAT: acinetobacter:ABUW_0001
RefSeq: WP_000964768.1
UniProt: A0A059ZTB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLWTDCLTRL RQELSDNVFA MWIRPLVAEE VEGILRLYAP NPYWTRYIQE NHLELISILA EQLSEGRVRQ VEILVDSRPG SILSSSEQPA TTTAALQTAP IPQPAKVKRE PEPVANTAVS SKSSKKKLLN PQFTFSLFVE GRSNQMAAET CRKVLTQLGA SQHNPLFLYG PTGLGKTHLM QAVGNALLQA KPNARVMYMT SESFVQDFVS SLQKGKVEEF KKNCRSLDLL LVDDIHLLAG KEASLVEFFY TFNALLDESK QIILTSDRYP KELTELDPRL VSRFSWGLSV GVEPPDIETR IEILLKKAEN SGVDLPRNCA LFIAQQVVAN VRELEGALNK VVAISRFKGA PIDLDVVRES LKDVLAIRAR TISVENIQRV VSEYFRIPLK ELVGPKRTRI YARPRQLAMG LARELTGDSF PEIGMAFGGR DHSTVMHACE KVVSLREEDP IFDEDYKNLL RLLQS
NT Sequence
ATGCTTTGGA CAGACTGCTT AACTCGCTTG CGACAAGAGC TCTCTGATAA CGTCTTTGCG ATGTGGATTC GCCCTTTAGT AGCTGAAGAA GTAGAGGGGA TACTACGTCT CTATGCTCCT AATCCTTATT GGACGCGTTA TATTCAAGAG AATCATTTAG AGTTAATTTC TATATTGGCT GAACAATTGT CGGAAGGGCG GGTGCGTCAG GTTGAAATCT TGGTAGATTC TCGTCCTGGT AGTATTTTGT CCTCTAGTGA ACAGCCTGCA ACAACTACAG CAGCTTTACA AACTGCCCCT ATACCTCAAC CTGCTAAGGT TAAAAGAGAA CCGGAACCTG TTGCTAATAC TGCAGTTAGT TCTAAGAGTT CAAAAAAGAA ACTATTAAAT CCACAATTTA CTTTTTCACT ATTTGTTGAA GGCCGTTCTA ACCAAATGGC AGCAGAAACC TGTAGAAAAG TATTAACACA GTTAGGTGCT TCTCAACATA ACCCTTTGTT TTTATATGGC CCGACAGGTC TTGGTAAGAC TCACTTAATG CAAGCAGTTG GTAATGCCTT ACTGCAAGCG AAGCCGAATG CAAGAGTCAT GTATATGACT TCAGAAAGTT TTGTACAAGA TTTTGTGAGC TCATTACAAA AAGGAAAGGT AGAAGAGTTT AAGAAAAATT GCCGTTCTTT AGACTTGTTA TTAGTAGATG ATATTCATCT TTTGGCAGGA AAAGAAGCAA GCCTCGTTGA ATTCTTCTAT ACATTTAATG CCTTACTTGA TGAATCCAAA CAAATTATTT TAACGTCAGA TCGATATCCT AAAGAATTAA CAGAACTTGA TCCTCGATTG GTTTCTCGTT TTTCCTGGGG ACTGTCAGTA GGTGTTGAAC CACCTGATAT TGAAACACGA ATCGAAATTC TGCTTAAAAA AGCTGAAAAT AGTGGCGTTG ATTTACCTAG AAACTGTGCG TTATTTATTG CCCAACAAGT CGTAGCGAAC GTACGTGAAC TTGAGGGCGC ACTGAATAAA GTTGTCGCAA TTTCACGTTT TAAAGGTGCT CCAATTGACC TTGATGTCGT ACGGGAATCT TTAAAAGATG TTTTAGCGAT CCGTGCTCGT ACAATTAGTG TAGAAAATAT CCAGCGTGTA GTGAGTGAAT ATTTCCGAAT TCCATTAAAA GAGCTGGTAG GTCCAAAGCG TACCCGAATT TATGCTCGAC CACGTCAGTT GGCGATGGGG CTTGCCCGTG AATTAACGGG GGATAGTTTT CCTGAAATTG GAATGGCTTT TGGTGGGCGT GATCACAGTA CAGTGATGCA TGCTTGTGAA AAAGTCGTCA GTTTACGGGA AGAAGACCCA ATCTTTGATG AAGATTATAA GAACTTATTA CGTTTGCTTC AAAGTTAA
Details for AcbaC.00941.a.B1.GE38667
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 53kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML WTDCLTRLRQ ELSDNVFAMW IRPLVAEEVE GILRLYAPNP YWTRYIQENH LELISILAEQ LSEGRVRQVE ILVDSRPGSI LSSSEQPATT TAALQTAPIP QPAKVKREPE PVANTAVSSK SSKKKLLNPQ FTFSLFVEGR SNQMAAETCR KVLTQLGASQ HNPLFLYGPT GLGKTHLMQA VGNALLQAKP NARVMYMTSE SFVQDFVSSL QKGKVEEFKK NCRSLDLLLV DDIHLLAGKE ASLVEFFYTF NALLDESKQI ILTSDRYPKE LTELDPRLVS RFSWGLSVGV EPPDIETRIE ILLKKAENSG VDLPRNCALF IAQQVVANVR ELEGALNKVV AISRFKGAPI DLDVVRESLK DVLAIRARTI SVENIQRVVS EYFRIPLKEL VGPKRTRIYA RPRQLAMGLA RELTGDSFPE IGMAFGGRDH STVMHACEKV VSLREEDPIF DEDYK
Validated NT Sequence
ttcttataat cttcatcaaa gattgggtct tcttcccgta aactgacgac tttttcacaa gcatgcatca ctgtactgtg atcacgccca ccaaaagcca ttccaatttc aggaaaacta tcccccgtta attcacgggc aagccccatc gccaactgac gtggtcgagc ataaattcgg gtacgctttg gacctaccag ctcttttaat ggaattcgga aatattcact cactacacgc tggatatttt ctacactaat tgtacgagca cggatcgcta aaacatcttt taaagattcc cgtacgacat caaggtcaat tggagcacct ttaaaacgtg aaattgcgac aactttattc agtgcgccct caagttcacg tacgttcgct acgacttgtt gggcaataaa taacgcacag tttctaggta aatcaacgcc actattttca gcttttttaa gcagaatttc gattcgtgtt tcaatatcag gtggttcaac acctactgac agtccccagg aaaaacgaga aaccaatcga ggatcaagtt ctgttaattc tttaggatat cgatctgacg ttaaaataat ttgtttggat tcatcaagta aggcattaaa tgtatagaag aattcaacga ggcttgcttc ttttcctgcc aaaagatgaa tatcatctac taataacaag tctaaagaac ggcaattttt cttaaactct tctacctttc ctttttgtaa tgagctcaca aaatcttgta caaaactttc tgaagtcata tacatgactc ttgcattcgg cttcgcttgc agtaaggcat taccaactgc ttgcattaag tgagtcttac caagacctgt cgggccatat aaaaacaaag ggttatgttg agaagcacct aactgtgtta atacttttct acaggtttct gctgccattt ggttagaacg gccttcaaca aatagtgaaa aagtaaattg tggatttaat agtttctttt ttgaactctt agaactaact gcagtattag caacaggttc cggttctctt ttaaccttag caggttgagg tataggggca gtttgtaaag ctgctgtagt tgttgcaggc tgttcactag aggacaaaat actaccagga cgagaatcta ccaagatttc aacctgacgc acccgccctt ccgacaattg ttcagccaat atagaaatta actctaaatg attctcttga atataacgcg tccaataagg attaggagca tagagacgta gtatcccctc tacttcttca gctactaaag ggcgaatcca catcgcaaag acgttatcag agagctcttg tcgcaagcga gttaagcagt ctgtccaaag catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHML WTDCLTRLRQ ELSDNVFAMW IRPLVAEEVE GILRLYAPNP YWTRYIQENH LELISILAEQ LSEGRVRQVE ILVDSRPGSI LSSSEQPATT TAALQTAPIP QPAKVKREPE PVANTAVSSK SSKKKLLNPQ FTFSLFVEGR SNQMAAETCR KVLTQLGASQ HNPLFLYGPT GLGKTHLMQA VGNALLQAKP NARVMYMTSE SFVQDFVSSL QKGKVEEFKK NCRSLDLLLV DDIHLLAGKE ASLVEFFYTF NALLDESKQI ILTSDRYPKE LTELDPRLVS RFSWGLSVGV EPPDIETRIE ILLKKAENSG VDLPRNCALF IAQQVVANVR ELEGALNKVV AISRFKGAPI DLDVVRESLK DVLAIRARTI SVENIQRVVS EYFRIPLKEL VGPKRTRIYA RPRQLAMGLA RELTGDSFPE IGMAFGGRDH STVMHACEKV VSLREEDPIF DEDYKNLLRL LQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttt ggacagactg cttaactcgc ttgcgacaag agctctctga taacgtcttt gcgatgtgga ttcgcccttt agtagctgaa gaagtagagg ggatactacg tctctatgct cctaatcctt attggacgcg ttatattcaa gagaatcatt tagagttaat ttctatattg gctgaacaat tgtcggaagg gcgggtgcgt caggttgaaa tcttggtaga ttctcgtcct ggtagtattt tgtcctctag tgaacagcct gcaacaacta cagcagcttt acaaactgcc cctatacctc aacctgctaa ggttaaaaga gaaccggaac ctgttgctaa tactgcagtt agttctaaga gttcaaaaaa gaaactatta aatccacaat ttactttttc actatttgtt gaaggccgtt ctaaccaaat ggcagcagaa acctgtagaa aagtattaac acagttaggt gcttctcaac ataacccttt gtttttatat ggcccgacag gtcttggtaa gactcactta atgcaagcag ttggtaatgc cttactgcaa gcgaagccga atgcaagagt catgtatatg acttcagaaa gttttgtaca agattttgtg agctcattac aaaaaggaaa ggtagaagag tttaagaaaa attgccgttc tttagacttg ttattagtag atgatattca tcttttggca ggaaaagaag caagcctcgt tgaattcttc tatacattta atgccttact tgatgaatcc aaacaaatta ttttaacgtc agatcgatat cctaaagaat taacagaact tgatcctcga ttggtttctc gtttttcctg gggactgtca gtaggtgttg aaccacctga tattgaaaca cgaatcgaaa ttctgcttaa aaaagctgaa aatagtggcg ttgatttacc tagaaactgt gcgttattta ttgcccaaca agtcgtagcg aacgtacgtg aacttgaggg cgcactgaat aaagttgtcg caatttcacg ttttaaaggt gctccaattg accttgatgt cgtacgggaa tctttaaaag atgttttagc gatccgtgct cgtacaatta gtgtagaaaa tatccagcgt gtagtgagtg aatatttccg aattccatta aaagagctgg taggtccaaa gcgtacccga atttatgctc gaccacgtca gttggcgatg gggcttgccc gtgaattaac gggggatagt tttcctgaaa ttggaatggc ttttggtggg cgtgatcaca gtacagtgat gcatgcttgt gaaaaagtcg tcagtttacg ggaagaagac ccaatctttg atgaagatta taagaactta ttacgtttgc ttcaaagttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat