AcbaC.00935.a

cysteine desulfurase IscS

CENTER ID: AcbaC.00935.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00935.a.B1.GE38666 full length 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00935.a.B1.PW37658 full length 1 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2157
GenBank: AKA31930.1
PGAT: acinetobacter:ABUW_2202
RefSeq: WP_000828390.1
UniProt: V5VDL9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRPIYLDYA ATTPVDPQVA ERMMECLTFD GTFGNAASRS HAYGWQAEEK VEYAREQVAN LIKADPREIV WTSGATESDN LALKGVAQFY ASKGKHIITS KIEHKAVLDP CRELEEQGFE ITYLEPEPQT GLITPEMVKA ALRPDTILVS LMMVNNEIGT VTDVAAIGEL TRANKTFFHV DAAQAAGKVD IDLSTMKIDL MSFSAHKIYG PKGIGALYVR RSPRVRLKAQ IHGGGHERGM RSGTLATHQI VGMGEAFELA GKTMHAEQER IRKLRDKLWN GLQDLEQVFL NGHPTQNVAN YLNVSFNFVE GESLMMSLKD AAVSSGSACT SATLEPSYVL RALGLSDELA HSSIRFSFGK YTTEEDIDHV LTITKAAVEK LRELSPLWDM YKEGIDLSTV EWAEH
NT Sequence
ATGAAACGTC CAATTTATCT TGATTACGCA GCAACCACTC CAGTAGATCC GCAAGTTGCA GAACGTATGA TGGAGTGTTT AACTTTCGAC GGTACCTTTG GTAATGCTGC ATCTCGTTCC CACGCTTATG GCTGGCAGGC AGAAGAAAAA GTTGAATATG CACGTGAGCA AGTCGCAAAC TTAATTAAAG CTGACCCACG TGAAATCGTT TGGACTTCTG GTGCAACTGA ATCAGACAAC CTTGCTCTTA AAGGTGTTGC TCAATTCTAT GCATCTAAAG GCAAACATAT CATTACAAGT AAAATTGAAC ATAAAGCTGT TCTCGATCCT TGCCGTGAAT TGGAAGAACA AGGTTTTGAA ATTACTTATT TAGAGCCAGA ACCACAAACA GGTTTAATTA CTCCTGAAAT GGTTAAAGCT GCCCTTCGCC CAGATACTAT TCTTGTTTCT CTTATGATGG TAAACAATGA AATTGGTACA GTTACAGATG TAGCAGCGAT CGGTGAATTA ACACGTGCAA ATAAAACGTT TTTCCATGTA GATGCAGCAC AAGCAGCTGG TAAAGTCGAT ATCGACTTAT CTACTATGAA AATTGACCTA ATGAGTTTCT CTGCTCATAA AATTTATGGT CCAAAAGGTA TCGGCGCATT ATATGTACGT CGTAGCCCAC GTGTTCGTCT AAAAGCACAA ATTCATGGCG GTGGACATGA ACGTGGTATG CGTTCTGGTA CTTTAGCGAC TCACCAAATT GTTGGTATGG GTGAAGCTTT TGAACTTGCA GGCAAAACAA TGCATGCAGA ACAAGAACGT ATTCGTAAAC TTCGTGACAA ACTTTGGAAC GGTTTACAAG ACCTTGAACA AGTATTCTTA AATGGCCACC CAACTCAAAA CGTTGCTAAC TATTTAAACG TCAGCTTCAA CTTTGTTGAA GGTGAATCTT TAATGATGTC GCTTAAAGAC GCTGCTGTAT CAAGTGGTTC TGCTTGTACT TCTGCTACTT TAGAGCCTTC ATATGTTCTT CGCGCATTAG GTTTATCTGA TGAGCTAGCA CACAGCTCAA TTCGCTTTAG TTTTGGTAAA TACACGACTG AAGAAGATAT TGACCACGTG CTTACAATTA CCAAAGCCGC TGTTGAGAAA TTACGTGAAC TTTCTCCGCT TTGGGATATG TACAAAGAAG GTATCGATCT TTCTACAGTT GAATGGGCAG AACACTAA
Details for AcbaC.00935.a.B1.GE38666
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK RPIYLDYAAT TPVDPQVAER MMECLTFDGT FGNAASRSHA YGWQAEEKVE YAREQVANLI KADPREIVWT SGATESDNLA LKGVAQFYAS KGKHIITSKI EHKAVLDPCR ELEEQGFEIT YLEPEPQTGL ITPEMVKAAL RPDTILVSLM MVNNEIGTVT DVAAIGELTR ANKTFFHVDA AQAAGKVDID LSTMKIDLMS FSAHKIYGPK GIGALYVRRS PRVRLKAQIH GGGHERGMRS GTLATHQIVG MGEAFELAGK TMHAEQERIR KLRDKLWNGL QDLEQVFLNG HPTQNVANYL NVSFNFVEGE SLMMSLKDAA VSSGSACTSA TLEPSYVLRA LGLSDELAHS SIRFSFGKYT TEEDIDHVLT ITKAAVEKLR ELSPLWDMYK EGIDL
Validated NT Sequence
agaaagatcg ataccttctt tgtacatatc ccaaagcgga gaaagttcac gtaatttctc aacagcggct ttggtaattg taagcacgtg gtcaatatct tcttcagtcg tgtatttacc aaaactaaag cgaattgagc tgtgtgctag ctcatcagat aaacctaatg cgcgaagaac atatgaaggc tctaaagtag cagaagtaca agcagaacca cttgatacag cagcgtcttt aagcgacatc attaaagatt caccttcaac aaagttgaag ctgacgttta aatagttagc aacgttttga gttgggtggc catttaagaa tacttgttca aggtcttgta aaccgttcca aagtttgtca cgaagtttac gaatacgttc ttgttctgca tgcattgttt tgcctgcaag ttcaaaagct tcacccatac caacaatttg gtgagtcgct aaagtaccag aacgcatacc acgttcatgt ccaccgccat gaatttgtgc ttttagacga acacgtgggc tacgacgtac atataatgcg ccgatacctt ttggaccata aattttatga gcagagaaac tcattaggtc aattttcata gtagataagt cgatatcgac tttaccagct gcttgtgctg catctacatg gaaaaacgtt ttatttgcac gtgttaattc accgatcgct gctacatctg taactgtacc aatttcattg tttaccatca taagagaaac aagaatagta tctgggcgaa gggcagcttt aaccatttca ggagtaatta aacctgtttg tggttctggc tctaaataag taatttcaaa accttgttct tccaattcac ggcaaggatc gagaacagct ttatgttcaa ttttacttgt aatgatatgt ttgcctttag atgcatagaa ttgagcaaca cctttaagag caaggttgtc tgattcagtt gcaccagaag tccaaacgat ttcacgtggg tcagctttaa ttaagtttgc gacttgctca cgtgcatatt caactttttc ttctgcctgc cagccataag cgtgggaacg agatgcagca ttaccaaagg taccgtcgaa agttaaacac tccatcatac gttctgcaac ttgcggatct actggagtgg ttgctgcgta atcaagataa attggacgtt tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMK RPIYLDYAAT TPVDPQVAER MMECLTFDGT FGNAASRSHA YGWQAEEKVE YAREQVANLI KADPREIVWT SGATESDNLA LKGVAQFYAS KGKHIITSKI EHKAVLDPCR ELEEQGFEIT YLEPEPQTGL ITPEMVKAAL RPDTILVSLM MVNNEIGTVT DVAAIGELTR ANKTFFHVDA AQAAGKVDID LSTMKIDLMS FSAHKIYGPK GIGALYVRRS PRVRLKAQIH GGGHERGMRS GTLATHQIVG MGEAFELAGK TMHAEQERIR KLRDKLWNGL QDLEQVFLNG HPTQNVANYL NVSFNFVEGE SLMMSLKDAA VSSGSACTSA TLEPSYVLRA LGLSDELAHS SIRFSFGKYT TEEDIDHVLT ITKAAVEKLR ELSPLWDMYK EGIDLSTVEW AEH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac gtccaattta tcttgattac gcagcaacca ctccagtaga tccgcaagtt gcagaacgta tgatggagtg tttaactttc gacggtacct ttggtaatgc tgcatctcgt tcccacgctt atggctggca ggcagaagaa aaagttgaat atgcacgtga gcaagtcgca aacttaatta aagctgaccc acgtgaaatc gtttggactt ctggtgcaac tgaatcagac aaccttgctc ttaaaggtgt tgctcaattc tatgcatcta aaggcaaaca tatcattaca agtaaaattg aacataaagc tgttctcgat ccttgccgtg aattggaaga acaaggtttt gaaattactt atttagagcc agaaccacaa acaggtttaa ttactcctga aatggttaaa gctgcccttc gcccagatac tattcttgtt tctcttatga tggtaaacaa tgaaattggt acagttacag atgtagcagc gatcggtgaa ttaacacgtg caaataaaac gtttttccat gtagatgcag cacaagcagc tggtaaagtc gatatcgact tatctactat gaaaattgac ctaatgagtt tctctgctca taaaatttat ggtccaaaag gtatcggcgc attatatgta cgtcgtagcc cacgtgttcg tctaaaagca caaattcatg gcggtggaca tgaacgtggt atgcgttctg gtactttagc gactcaccaa attgttggta tgggtgaagc ttttgaactt gcaggcaaaa caatgcatgc agaacaagaa cgtattcgta aacttcgtga caaactttgg aacggtttac aagaccttga acaagtattc ttaaatggcc acccaactca aaacgttgct aactatttaa acgtcagctt caactttgtt gaaggtgaat ctttaatgat gtcgcttaaa gacgctgctg tatcaagtgg ttctgcttgt acttctgcta ctttagagcc ttcatatgtt cttcgcgcat taggtttatc tgatgagcta gcacacagct caattcgctt tagttttggt aaatacacga ctgaagaaga tattgaccac gtgcttacaa ttaccaaagc cgctgttgag aaattacgtg aactttctcc gctttgggat atgtacaaag aaggtatcga tctttctaca gttgaatggg cagaacactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for AcbaC.00935.a.B1.PW37658
PURIFICATION DATe: 2/25/2015
CONCENTRATION: 31mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 23
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK RPIYLDYAAT TPVDPQVAER MMECLTFDGT FGNAASRSHA YGWQAEEKVE YAREQVANLI KADPREIVWT SGATESDNLA LKGVAQFYAS KGKHIITSKI EHKAVLDPCR ELEEQGFEIT YLEPEPQTGL ITPEMVKAAL RPDTILVSLM MVNNEIGTVT DVAAIGELTR ANKTFFHVDA AQAAGKVDID LSTMKIDLMS FSAHKIYGPK GIGALYVRRS PRVRLKAQIH GGGHERGMRS GTLATHQIVG MGEAFELAGK TMHAEQERIR KLRDKLWNGL QDLEQVFLNG HPTQNVANYL NVSFNFVEGE SLMMSLKDAA VSSGSACTSA TLEPSYVLRA LGLSDELAHS SIRFSFGKYT TEEDIDHVLT ITKAAVEKLR ELSPLWDMYK EGIDL
Validated NT Sequence
agaaagatcg ataccttctt tgtacatatc ccaaagcgga gaaagttcac gtaatttctc aacagcggct ttggtaattg taagcacgtg gtcaatatct tcttcagtcg tgtatttacc aaaactaaag cgaattgagc tgtgtgctag ctcatcagat aaacctaatg cgcgaagaac atatgaaggc tctaaagtag cagaagtaca agcagaacca cttgatacag cagcgtcttt aagcgacatc attaaagatt caccttcaac aaagttgaag ctgacgttta aatagttagc aacgttttga gttgggtggc catttaagaa tacttgttca aggtcttgta aaccgttcca aagtttgtca cgaagtttac gaatacgttc ttgttctgca tgcattgttt tgcctgcaag ttcaaaagct tcacccatac caacaatttg gtgagtcgct aaagtaccag aacgcatacc acgttcatgt ccaccgccat gaatttgtgc ttttagacga acacgtgggc tacgacgtac atataatgcg ccgatacctt ttggaccata aattttatga gcagagaaac tcattaggtc aattttcata gtagataagt cgatatcgac tttaccagct gcttgtgctg catctacatg gaaaaacgtt ttatttgcac gtgttaattc accgatcgct gctacatctg taactgtacc aatttcattg tttaccatca taagagaaac aagaatagta tctgggcgaa gggcagcttt aaccatttca ggagtaatta aacctgtttg tggttctggc tctaaataag taatttcaaa accttgttct tccaattcac ggcaaggatc gagaacagct ttatgttcaa ttttacttgt aatgatatgt ttgcctttag atgcatagaa ttgagcaaca cctttaagag caaggttgtc tgattcagtt gcaccagaag tccaaacgat ttcacgtggg tcagctttaa ttaagtttgc gacttgctca cgtgcatatt caactttttc ttctgcctgc cagccataag cgtgggaacg agatgcagca ttaccaaagg taccgtcgaa agttaaacac tccatcatac gttctgcaac ttgcggatct actggagtgg ttgctgcgta atcaagataa attggacgtt tcatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMK RPIYLDYAAT TPVDPQVAER MMECLTFDGT FGNAASRSHA YGWQAEEKVE YAREQVANLI KADPREIVWT SGATESDNLA LKGVAQFYAS KGKHIITSKI EHKAVLDPCR ELEEQGFEIT YLEPEPQTGL ITPEMVKAAL RPDTILVSLM MVNNEIGTVT DVAAIGELTR ANKTFFHVDA AQAAGKVDID LSTMKIDLMS FSAHKIYGPK GIGALYVRRS PRVRLKAQIH GGGHERGMRS GTLATHQIVG MGEAFELAGK TMHAEQERIR KLRDKLWNGL QDLEQVFLNG HPTQNVANYL NVSFNFVEGE SLMMSLKDAA VSSGSACTSA TLEPSYVLRA LGLSDELAHS SIRFSFGKYT TEEDIDHVLT ITKAAVEKLR ELSPLWDMYK EGIDLSTVEW AEH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC GTCCAATTTA TCTTGATTAC GCAGCAACCA CTCCAGTAGA TCCGCAAGTT GCAGAACGTA TGATGGAGTG TTTAACTTTC GACGGTACCT TTGGTAATGC TGCATCTCGT TCCCACGCTT ATGGCTGGCA GGCAGAAGAA AAAGTTGAAT ATGCACGTGA GCAAGTCGCA AACTTAATTA AAGCTGACCC ACGTGAAATC GTTTGGACTT CTGGTGCAAC TGAATCAGAC AACCTTGCTC TTAAAGGTGT TGCTCAATTC TATGCATCTA AAGGCAAACA TATCATTACA AGTAAAATTG AACATAAAGC TGTTCTCGAT CCTTGCCGTG AATTGGAAGA ACAAGGTTTT GAAATTACTT ATTTAGAGCC AGAACCACAA ACAGGTTTAA TTACTCCTGA AATGGTTAAA GCTGCCCTTC GCCCAGATAC TATTCTTGTT TCTCTTATGA TGGTAAACAA TGAAATTGGT ACAGTTACAG ATGTAGCAGC GATCGGTGAA TTAACACGTG CAAATAAAAC GTTTTTCCAT GTAGATGCAG CACAAGCAGC TGGTAAAGTC GATATCGACT TATCTACTAT GAAAATTGAC CTAATGAGTT TCTCTGCTCA TAAAATTTAT GGTCCAAAAG GTATCGGCGC ATTATATGTA CGTCGTAGCC CACGTGTTCG TCTAAAAGCA CAAATTCATG GCGGTGGACA TGAACGTGGT ATGCGTTCTG GTACTTTAGC GACTCACCAA ATTGTTGGTA TGGGTGAAGC TTTTGAACTT GCAGGCAAAA CAATGCATGC AGAACAAGAA CGTATTCGTA AACTTCGTGA CAAACTTTGG AACGGTTTAC AAGACCTTGA ACAAGTATTC TTAAATGGCC ACCCAACTCA AAACGTTGCT AACTATTTAA ACGTCAGCTT CAACTTTGTT GAAGGTGAAT CTTTAATGAT GTCGCTTAAA GACGCTGCTG TATCAAGTGG TTCTGCTTGT ACTTCTGCTA CTTTAGAGCC TTCATATGTT CTTCGCGCAT TAGGTTTATC TGATGAGCTA GCACACAGCT CAATTCGCTT TAGTTTTGGT AAATACACGA CTGAAGAAGA TATTGACCAC GTGCTTACAA TTACCAAAGC CGCTGTTGAG AAATTACGTG AACTTTCTCC GCTTTGGGAT ATGTACAAAG AAGGTATCGA TCTTTCTACA GTTGAATGGG CAGAACACTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT