AcbaC.00914.a

glyceraldehyde-3-phosphate dehydrogenase

CENTER ID: AcbaC.00914.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00914.a.B1.GE38665 full length 1 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00914.a.B1.PW37681 full length 1 485

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1288.peg.1246
GenBank: AKA30803.1
PGAT: acinetobacter:ABUW_1050
RefSeq: WP_000031228.1
UniProt: A0A059ZNN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VSKDTIIALH AEHQGRWKNR EEIAERMIAL IGQLYREKNI VVSVYGRSLI NRSVIQILKT HRRTRVVDVE LSVVNTFPIL EALAKVENIG SAEVDIGKLA VEYKEKGGDV DAFVAQAVES IKGSATSEQP KDVVLYGFGR IGRILARLII SQSGLGRGLS LKAIVVRKSS DGDLAKRASL LRRDSIHGTF AGTISVDEEN EAIIANGNFI KVIYASSPSE VDYTQYGIEN ALLIDNTGKW RDAEGLSQHL KCPGVARVVL TAPSKGEMKN VVFGVNNSDI LDEDKIISAA SCTTNAITPI LKVLDDKYKV LNGHVETVHS FTNDQNLIDN YHKADRRGRA ATLNMVITET GAAKAVAKAL PALKGKLTGN SVRVPTPNVS LAILNLTLDK EVDREEVNEY IRQISINSNL QGQIGYTNST EVVSSDFIGS RTAGVYDAQA TITSGNRLTA YVWYDNEVGY SCQVLRIAEQ MCGVSYKKIP AETNA
NT Sequence
GTGAGCAAAG ACACTATCAT CGCCCTGCAC GCTGAACACC AAGGCCGTTG GAAAAACCGT GAAGAAATCG CGGAACGTAT GATTGCATTA ATCGGTCAAT TATACCGTGA AAAAAATATT GTCGTATCTG TTTACGGTCG CTCTTTAATT AACCGTTCTG TTATTCAAAT TTTGAAAACT CACCGTCGTA CACGTGTTGT AGATGTTGAG CTTTCTGTAG TAAATACTTT CCCAATTTTA GAAGCTTTAG CAAAAGTTGA AAATATCGGT TCTGCAGAAG TTGATATCGG TAAACTTGCA GTTGAATATA AAGAAAAAGG CGGTGATGTT GACGCTTTTG TTGCTCAAGC TGTTGAGTCA ATTAAAGGTA GCGCAACTTC TGAACAACCT AAAGATGTAG TTCTTTACGG TTTTGGTCGT ATTGGCCGTA TCTTGGCTCG TTTAATCATT AGCCAATCAG GTTTAGGTCG TGGTTTAAGC CTTAAAGCGA TTGTTGTTCG TAAGTCTTCT GACGGTGACT TGGCTAAACG TGCTTCTTTA CTTCGTCGTG ACTCTATTCA CGGTACTTTC GCTGGTACAA TTTCTGTTGA TGAAGAAAAC GAAGCAATTA TCGCTAACGG TAACTTCATC AAAGTAATCT ATGCATCTAG CCCAAGCGAA GTAGACTATA CTCAATACGG TATTGAAAAC GCGCTTTTAA TCGACAACAC TGGTAAATGG CGTGATGCTG AAGGCCTAAG CCAACACTTG AAATGCCCAG GTGTTGCACG TGTTGTATTA ACTGCACCAA GTAAAGGCGA AATGAAAAAC GTTGTATTTG GTGTAAACAA CTCTGACATT TTAGATGAAG ACAAAATCAT CTCTGCTGCA AGTTGTACAA CAAATGCGAT CACTCCAATT CTTAAAGTAT TGGATGACAA ATATAAAGTT CTTAATGGTC ACGTTGAGAC AGTTCACTCA TTTACAAATG ACCAGAACTT GATCGATAAC TACCACAAAG CTGATCGTCG TGGTCGTGCT GCTACATTGA ACATGGTTAT TACTGAAACT GGTGCTGCTA AAGCTGTTGC TAAAGCATTG CCTGCGTTAA AAGGTAAGTT AACTGGTAAC TCTGTACGTG TTCCAACACC AAACGTATCT CTTGCGATTT TAAACTTAAC GCTTGATAAA GAAGTTGATC GTGAAGAAGT GAACGAATAC ATTCGTCAAA TTTCGATCAA CTCTAACCTT CAAGGTCAAA TCGGTTATAC CAACTCTACA GAAGTGGTAT CAAGCGACTT TATCGGTTCT CGTACTGCTG GTGTATATGA TGCTCAAGCA ACGATTACTT CTGGTAACCG CTTAACTGCA TATGTTTGGT ACGATAATGA AGTAGGTTAT AGCTGCCAAG TATTACGTAT TGCTGAGCAA ATGTGTGGCG TAAGCTATAA AAAAATTCCT GCAGAAACTA ACGCTTAA
Details for AcbaC.00914.a.B1.GE38665
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHH MVSKDTIIAL HAEHQGRWKN REEIAERMIA LIGQLYREKN IVVSVYGRSL INRSVIQILK THRRTRVVDV ELSVVNTFPI LEALAKVENI GSAEVDIGKL AVEYKEKGGD VDAFVAQAVE SIKGSATSEQ PKDVVLYGFG RIGRILARLI ISQSGLGRGL SLKAIVVRKS SDGDLAKRAS LLRRDSIHGT FAGTISVDEE NEAIIANGNF IKVIYASSPS EVDYTQYGIE NALLIDNTGK WRDAEGLSQH LKCPGVARVV LTAPSKGEMK NVVFGVNNSD ILDEDKIISA ASCTTNAITP ILKVLDDKYK VLNGHVETVH SFTNDQNLID NYHKADRRGR AATLNMVITE TGAAKAVAKA LPALKGKLTG NSVRVPTPNV SLAILNLTLD KEVDREEVNE YIRQISINSN LQGQIGYTNS TEVVSSDFIG SRTAGVYDAQ ATITSGNRLT AYVWYDNEVG YSCQVLRIAE QMCGVSYK
Validated NT Sequence
ttttttatag cttacgccac acatttgctc agcaatacgt aatacttggc agctataacc tacttcatta tcgtaccaaa catatgcagt taagcggtta ccagaagtaa tcgttgcttg agcatcatat acaccagcag tacgagaacc gataaagtcg cttgatacca cttctgtaga gttggtataa ccgatttgac cttgaaggtt agagttgatc gaaatttgac gaatgtattc gttcacttct tcacgatcaa cttctttatc aagcgttaag tttaaaatcg caagagatac gtttggtgtt ggaacacgta cagagttacc agttaactta ccttttaacg caggcaatgc tttagcaaca gctttagcag caccagtttc agtaataacc atgttcaatg tagcagcacg accacgacga tcagctttgt ggtagttatc gatcaagttc tggtcatttg taaatgagtg aactgtctca acgtgaccat taagaacttt atatttgtca tccaatactt taagaattgg agtgatcgca tttgttgtac aacttgcagc agagatgatt ttgtcttcat ctaaaatgtc agagttgttt acaccaaata caacgttttt catttcgcct ttacttggtg cagttaatac aacacgtgca acacctgggc atttcaagtg ttggcttagg ccttcagcat cacgccattt accagtgttg tcgattaaaa gcgcgttttc aataccgtat tgagtatagt ctacttcgct tgggctagat gcatagatta ctttgatgaa gttaccgtta gcgataattg cttcgttttc ttcatcaaca gaaattgtac cagcgaaagt accgtgaata gagtcacgac gaagtaaaga agcacgttta gccaagtcac cgtcagaaga cttacgaaca acaatcgctt taaggcttaa accacgacct aaacctgatt ggctaatgat taaacgagcc aagatacggc caatacgacc aaaaccgtaa agaactacat ctttaggttg ttcagaagtt gcgctacctt taattgactc aacagcttga gcaacaaaag cgtcaacatc accgcctttt tctttatatt caactgcaag tttaccgata tcaacttctg cagaaccgat attttcaact tttgctaaag cttctaaaat tgggaaagta tttactacag aaagctcaac atctacaaca cgtgtacgac ggtgagtttt caaaatttga ataacagaac ggttaattaa agagcgaccg taaacagata cgacaatatt tttttcacgg tataattgac cgattaatgc aatcatacgt tccgcgattt cttcacggtt tttccaacgg ccttggtgtt cagcgtgcag ggcgatgata gtgtctttgc tcaccatatg gtggtggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHVS KDTIIALHAE HQGRWKNREE IAERMIALIG QLYREKNIVV SVYGRSLINR SVIQILKTHR RTRVVDVELS VVNTFPILEA LAKVENIGSA EVDIGKLAVE YKEKGGDVDA FVAQAVESIK GSATSEQPKD VVLYGFGRIG RILARLIISQ SGLGRGLSLK AIVVRKSSDG DLAKRASLLR RDSIHGTFAG TISVDEENEA IIANGNFIKV IYASSPSEVD YTQYGIENAL LIDNTGKWRD AEGLSQHLKC PGVARVVLTA PSKGEMKNVV FGVNNSDILD EDKIISAASC TTNAITPILK VLDDKYKVLN GHVETVHSFT NDQNLIDNYH KADRRGRAAT LNMVITETGA AKAVAKALPA LKGKLTGNSV RVPTPNVSLA ILNLTLDKEV DREEVNEYIR QISINSNLQG QIGYTNSTEV VSSDFIGSRT AGVYDAQATI TSGNRLTAYV WYDNEVGYSC QVLRIAEQMC GVSYKKIPAE TNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagca aagacactat catcgccctg cacgctgaac accaaggccg ttggaaaaac cgtgaagaaa tcgcggaacg tatgattgca ttaatcggtc aattataccg tgaaaaaaat attgtcgtat ctgtttacgg tcgctcttta attaaccgtt ctgttattca aattttgaaa actcaccgtc gtacacgtgt tgtagatgtt gagctttctg tagtaaatac tttcccaatt ttagaagctt tagcaaaagt tgaaaatatc ggttctgcag aagttgatat cggtaaactt gcagttgaat ataaagaaaa aggcggtgat gttgacgctt ttgttgctca agctgttgag tcaattaaag gtagcgcaac ttctgaacaa cctaaagatg tagttcttta cggttttggt cgtattggcc gtatcttggc tcgtttaatc attagccaat caggtttagg tcgtggttta agccttaaag cgattgttgt tcgtaagtct tctgacggtg acttggctaa acgtgcttct ttacttcgtc gtgactctat tcacggtact ttcgctggta caatttctgt tgatgaagaa aacgaagcaa ttatcgctaa cggtaacttc atcaaagtaa tctatgcatc tagcccaagc gaagtagact atactcaata cggtattgaa aacgcgcttt taatcgacaa cactggtaaa tggcgtgatg ctgaaggcct aagccaacac ttgaaatgcc caggtgttgc acgtgttgta ttaactgcac caagtaaagg cgaaatgaaa aacgttgtat ttggtgtaaa caactctgac attttagatg aagacaaaat catctctgct gcaagttgta caacaaatgc gatcactcca attcttaaag tattggatga caaatataaa gttcttaatg gtcacgttga gacagttcac tcatttacaa atgaccagaa cttgatcgat aactaccaca aagctgatcg tcgtggtcgt gctgctacat tgaacatggt tattactgaa actggtgctg ctaaagctgt tgctaaagca ttgcctgcgt taaaaggtaa gttaactggt aactctgtac gtgttccaac accaaacgta tctcttgcga ttttaaactt aacgcttgat aaagaagttg atcgtgaaga agtgaacgaa tacattcgtc aaatttcgat caactctaac cttcaaggtc aaatcggtta taccaactct acagaagtgg tatcaagcga ctttatcggt tctcgtactg ctggtgtata tgatgctcaa gcaacgatta cttctggtaa ccgcttaact gcatatgttt ggtacgataa tgaagtaggt tatagctgcc aagtattacg tattgctgag caaatgtgtg gcgtaagcta taaaaaaatt cctgcagaaa ctaacgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for AcbaC.00914.a.B1.PW37681
PURIFICATION DATe: 3/25/2015
CONCENTRATION: 27mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 22
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHH MVSKDTIIAL HAEHQGRWKN REEIAERMIA LIGQLYREKN IVVSVYGRSL INRSVIQILK THRRTRVVDV ELSVVNTFPI LEALAKVENI GSAEVDIGKL AVEYKEKGGD VDAFVAQAVE SIKGSATSEQ PKDVVLYGFG RIGRILARLI ISQSGLGRGL SLKAIVVRKS SDGDLAKRAS LLRRDSIHGT FAGTISVDEE NEAIIANGNF IKVIYASSPS EVDYTQYGIE NALLIDNTGK WRDAEGLSQH LKCPGVARVV LTAPSKGEMK NVVFGVNNSD ILDEDKIISA ASCTTNAITP ILKVLDDKYK VLNGHVETVH SFTNDQNLID NYHKADRRGR AATLNMVITE TGAAKAVAKA LPALKGKLTG NSVRVPTPNV SLAILNLTLD KEVDREEVNE YIRQISINSN LQGQIGYTNS TEVVSSDFIG SRTAGVYDAQ ATITSGNRLT AYVWYDNEVG YSCQVLRIAE QMCGVSYK
Validated NT Sequence
ttttttatag cttacgccac acatttgctc agcaatacgt aatacttggc agctataacc tacttcatta tcgtaccaaa catatgcagt taagcggtta ccagaagtaa tcgttgcttg agcatcatat acaccagcag tacgagaacc gataaagtcg cttgatacca cttctgtaga gttggtataa ccgatttgac cttgaaggtt agagttgatc gaaatttgac gaatgtattc gttcacttct tcacgatcaa cttctttatc aagcgttaag tttaaaatcg caagagatac gtttggtgtt ggaacacgta cagagttacc agttaactta ccttttaacg caggcaatgc tttagcaaca gctttagcag caccagtttc agtaataacc atgttcaatg tagcagcacg accacgacga tcagctttgt ggtagttatc gatcaagttc tggtcatttg taaatgagtg aactgtctca acgtgaccat taagaacttt atatttgtca tccaatactt taagaattgg agtgatcgca tttgttgtac aacttgcagc agagatgatt ttgtcttcat ctaaaatgtc agagttgttt acaccaaata caacgttttt catttcgcct ttacttggtg cagttaatac aacacgtgca acacctgggc atttcaagtg ttggcttagg ccttcagcat cacgccattt accagtgttg tcgattaaaa gcgcgttttc aataccgtat tgagtatagt ctacttcgct tgggctagat gcatagatta ctttgatgaa gttaccgtta gcgataattg cttcgttttc ttcatcaaca gaaattgtac cagcgaaagt accgtgaata gagtcacgac gaagtaaaga agcacgttta gccaagtcac cgtcagaaga cttacgaaca acaatcgctt taaggcttaa accacgacct aaacctgatt ggctaatgat taaacgagcc aagatacggc caatacgacc aaaaccgtaa agaactacat ctttaggttg ttcagaagtt gcgctacctt taattgactc aacagcttga gcaacaaaag cgtcaacatc accgcctttt tctttatatt caactgcaag tttaccgata tcaacttctg cagaaccgat attttcaact tttgctaaag cttctaaaat tgggaaagta tttactacag aaagctcaac atctacaaca cgtgtacgac ggtgagtttt caaaatttga ataacagaac ggttaattaa agagcgaccg taaacagata cgacaatatt tttttcacgg tataattgac cgattaatgc aatcatacgt tccgcgattt cttcacggtt tttccaacgg ccttggtgtt cagcgtgcag ggcgatgata gtgtctttgc tcaccatatg gtggtggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHVS KDTIIALHAE HQGRWKNREE IAERMIALIG QLYREKNIVV SVYGRSLINR SVIQILKTHR RTRVVDVELS VVNTFPILEA LAKVENIGSA EVDIGKLAVE YKEKGGDVDA FVAQAVESIK GSATSEQPKD VVLYGFGRIG RILARLIISQ SGLGRGLSLK AIVVRKSSDG DLAKRASLLR RDSIHGTFAG TISVDEENEA IIANGNFIKV IYASSPSEVD YTQYGIENAL LIDNTGKWRD AEGLSQHLKC PGVARVVLTA PSKGEMKNVV FGVNNSDILD EDKIISAASC TTNAITPILK VLDDKYKVLN GHVETVHSFT NDQNLIDNYH KADRRGRAAT LNMVITETGA AKAVAKALPA LKGKLTGNSV RVPTPNVSLA ILNLTLDKEV DREEVNEYIR QISINSNLQG QIGYTNSTEV VSSDFIGSRT AGVYDAQATI TSGNRLTAYV WYDNEVGYSC QVLRIAEQMC GVSYKKIPAE TNA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCA AAGACACTAT CATCGCCCTG CACGCTGAAC ACCAAGGCCG TTGGAAAAAC CGTGAAGAAA TCGCGGAACG TATGATTGCA TTAATCGGTC AATTATACCG TGAAAAAAAT ATTGTCGTAT CTGTTTACGG TCGCTCTTTA ATTAACCGTT CTGTTATTCA AATTTTGAAA ACTCACCGTC GTACACGTGT TGTAGATGTT GAGCTTTCTG TAGTAAATAC TTTCCCAATT TTAGAAGCTT TAGCAAAAGT TGAAAATATC GGTTCTGCAG AAGTTGATAT CGGTAAACTT GCAGTTGAAT ATAAAGAAAA AGGCGGTGAT GTTGACGCTT TTGTTGCTCA AGCTGTTGAG TCAATTAAAG GTAGCGCAAC TTCTGAACAA CCTAAAGATG TAGTTCTTTA CGGTTTTGGT CGTATTGGCC GTATCTTGGC TCGTTTAATC ATTAGCCAAT CAGGTTTAGG TCGTGGTTTA AGCCTTAAAG CGATTGTTGT TCGTAAGTCT TCTGACGGTG ACTTGGCTAA ACGTGCTTCT TTACTTCGTC GTGACTCTAT TCACGGTACT TTCGCTGGTA CAATTTCTGT TGATGAAGAA AACGAAGCAA TTATCGCTAA CGGTAACTTC ATCAAAGTAA TCTATGCATC TAGCCCAAGC GAAGTAGACT ATACTCAATA CGGTATTGAA AACGCGCTTT TAATCGACAA CACTGGTAAA TGGCGTGATG CTGAAGGCCT AAGCCAACAC TTGAAATGCC CAGGTGTTGC ACGTGTTGTA TTAACTGCAC CAAGTAAAGG CGAAATGAAA AACGTTGTAT TTGGTGTAAA CAACTCTGAC ATTTTAGATG AAGACAAAAT CATCTCTGCT GCAAGTTGTA CAACAAATGC GATCACTCCA ATTCTTAAAG TATTGGATGA CAAATATAAA GTTCTTAATG GTCACGTTGA GACAGTTCAC TCATTTACAA ATGACCAGAA CTTGATCGAT AACTACCACA AAGCTGATCG TCGTGGTCGT GCTGCTACAT TGAACATGGT TATTACTGAA ACTGGTGCTG CTAAAGCTGT TGCTAAAGCA TTGCCTGCGT TAAAAGGTAA GTTAACTGGT AACTCTGTAC GTGTTCCAAC ACCAAACGTA TCTCTTGCGA TTTTAAACTT AACGCTTGAT AAAGAAGTTG ATCGTGAAGA AGTGAACGAA TACATTCGTC AAATTTCGAT CAACTCTAAC CTTCAAGGTC AAATCGGTTA TACCAACTCT ACAGAAGTGG TATCAAGCGA CTTTATCGGT TCTCGTACTG CTGGTGTATA TGATGCTCAA GCAACGATTA CTTCTGGTAA CCGCTTAACT GCATATGTTT GGTACGATAA TGAAGTAGGT TATAGCTGCC AAGTATTACG TATTGCTGAG CAAATGTGTG GCGTAAGCTA TAAAAAAATT CCTGCAGAAA CTAACGCTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT