AcbaC.00888.a

adenylosuccinate synthetase

CENTER ID: AcbaC.00888.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00888.a.B1.GE43937 full length 1 439
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2698
GenBank: AKA32462.1
PGAT: acinetobacter:ABUW_2745
RefSeq: WP_000517792.1
UniProt: A0A0Q1T6Y1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKNVVVLGT QWGDEGKGKI VDLLTDQAAA VVRYQGGHNA GHTLVVGGKK TVLHLIPSGI LRENVLCLIG NGVVLSPAAL IEEMGILEAE GVPVKERLRI SPNCPLILPN HIALDQAREK KRGNAKIGTT GRGIGPAYED KVARRAVRVA DLVRGGAALE EKLAEMLELH NFQLTQFYGV EAVKFEDVLA LCNQWREVLA PLVIDVTKVL HDYRKEGKAV MFEGAQGSLL DIDHGTYPYV TSSNTTAGGV SSGSGMGPLH LDYVLGITKA YTTRVGAGPF PTELVYDAAS DSGDPIGKHL GTVGHEFGAS TGRQRRCGWF DAEILRRSVD VNSLSGICLT KLDVLDGLEE VKICVGYENT DSGCVGSSDA VSFECLKPIY ETMPGWSEST VGLTSIDQLP ANALAYVKRI EQLIECPIDI VSTGPDRAET IILRHPFSA
NT Sequence
ATGGGCAAAA ATGTTGTGGT ACTTGGTACC CAATGGGGCG ACGAAGGTAA AGGTAAAATC GTCGACCTGC TCACAGATCA GGCGGCTGCG GTAGTACGTT ATCAAGGCGG ACATAACGCA GGTCATACTC TTGTCGTAGG TGGTAAAAAG ACTGTATTAC ACCTCATTCC ATCAGGCATT TTACGTGAAA ACGTATTGTG CTTAATTGGC AATGGCGTGG TGCTTTCACC AGCAGCATTA ATTGAAGAAA TGGGAATTTT GGAAGCTGAG GGTGTTCCGG TTAAGGAGCG TCTACGCATT TCTCCAAACT GTCCACTTAT TTTGCCTAAC CACATTGCTC TTGATCAGGC ACGTGAGAAG AAACGTGGTA ACGCTAAAAT CGGTACTACA GGTCGTGGTA TCGGTCCTGC GTACGAAGAT AAAGTTGCGC GCCGTGCAGT GCGTGTTGCT GACCTTGTTC GTGGTGGTGC AGCGTTAGAA GAAAAACTAG CCGAAATGCT AGAGTTACAT AACTTCCAAT TGACTCAGTT CTACGGTGTA GAAGCGGTTA AATTTGAAGA CGTATTGGCT CTTTGCAACC AATGGCGTGA AGTTCTTGCT CCATTAGTGA TTGACGTAAC TAAAGTATTA CATGACTACC GCAAAGAAGG TAAGGCTGTA ATGTTTGAAG GTGCTCAAGG TTCACTTCTA GACATCGACC ACGGTACTTA TCCTTATGTT ACCAGCTCAA ATACAACTGC TGGTGGTGTA AGTTCTGGTT CAGGTATGGG TCCTTTACAT CTTGATTATG TATTAGGTAT TACGAAAGCT TATACAACAC GTGTAGGTGC TGGTCCGTTC CCAACTGAAT TGGTTTACGA TGCTGCAAGT GATTCTGGTG ACCCAATTGG TAAACATTTG GGTACTGTGG GTCATGAGTT CGGTGCTTCT ACTGGTCGAC AACGTCGTTG TGGTTGGTTT GATGCTGAAA TCTTACGCCG TTCTGTAGAT GTAAACTCAC TTTCAGGTAT TTGCTTAACT AAACTTGACG TTTTAGATGG TTTAGAAGAA GTGAAAATCT GTGTGGGTTA TGAAAATACA GATTCAGGTT GTGTAGGTTC TTCTGATGCG GTTTCTTTCG AATGCTTGAA ACCAATTTAT GAAACTATGC CAGGTTGGAG CGAGTCAACT GTTGGTTTAA CGAGCATTGA CCAATTACCT GCAAATGCTT TGGCTTATGT AAAACGTATT GAACAATTAA TCGAATGTCC GATTGATATC GTTTCAACTG GTCCAGACCG TGCAGAAACA ATCATTTTAC GTCATCCATT CTCTGCGTAA
Details for AcbaC.00888.a.B1.GE43937
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 47
PERCENT COVERAGE: 8
Validated AA Sequence
MAHHHHHHMA KMLWYLVPNG ATKVKVKSST CSQIRRLR
Validated NT Sequence
atcttactca cttacgcaga gaatggatga cgtaaaatga ttgtttctgc acggtctgga ccagttgaaa cgatatcaat cggacattcg attaattgtt caatacgttt tacataagcc aaagcatttg caggtaattg gtcaatgctc gttaaaccaa cagttgactc gctccaacct ggcatagttt cataaattgg tttcaagcat tcgaaagaaa ccgcatcaga agaacctaca caacctgaat ctgtattttc ataacccaca cagattttca cttcttctaa accatctaaa acgtcaagtt tagttaagca aatacctgaa agtgagttta catctacaga acggcgtaag atttcagcat caaaccaacc acaacgacgt tgtcgaccag tagaagcacc gaactcatga cccacagtac ccaaatgttt accaattggg tcaccagaat cacttgcagc atcgtaaacc aattcagttg ggaacggacc agcacctaca cgtgttgtat aagctttcgt aatacctaat acataatcaa gatgtaaagg acccatacct gaaccagaac ttacaccacc agcagttgta tttgagctgg taacataagg ataagtaccg tggtcgatgt ctagaagtga accttgagca ccttcaaaca ttacagcctt accttctttg cggtagtcat gtaatacttt agttacgtca atcactaatg gagcaagaac ttcacgccat tggttgcaaa gagccaatac gtcttcaaat ttaaccgctt ctacaccgta gaactgagtc aattggaagt tatgtaactc tagcatttcg gctagttttt cttctaacgc tgcaccacca cgaacaaggt cagcaacacg cactgcacgg cgcgcaactt tatcttcgta cgcaggaccg ataccacgac ctgtagtacc gattttagcg ttaccacgtt tcttctcacg tgcctgatca agagcaatgt ggttaggcaa aataagtgga cagtttggag aaatgcgtag acgctcctta accggaacac cctcagcttc caaaattccc atttcttcaa ttaatgctgc tggtgaaagc accacgccat tgccaattaa gcacaatacg ttttcacgta aaatgcctga tggaatgagg tgtaatacag tctttttacc acctacgaca agagtatgac ctgcgttatg tccgccttga taacgtacta ccgcagccgc ctgatctgtg agcaggtcga cgattttacc tttaccttcg tcgccccatt gggtaccaag taccacaaca tttttgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMG KNVVVLGTQW GDEGKGKIVD LLTDQAAAVV RYQGGHNAGH TLVVGGKKTV LHLIPSGILR ENVLCLIGNG VVLSPAALIE EMGILEAEGV PVKERLRISP NCPLILPNHI ALDQAREKKR GNAKIGTTGR GIGPAYEDKV ARRAVRVADL VRGGAALEEK LAEMLELHNF QLTQFYGVEA VKFEDVLALC NQWREVLAPL VIDVTKVLHD YRKEGKAVMF EGAQGSLLDI DHGTYPYVTS SNTTAGGVSS GSGMGPLHLD YVLGITKAYT TRVGAGPFPT ELVYDAASDS GDPIGKHLGT VGHEFGASTG RQRRCGWFDA EILRRSVDVN SLSGICLTKL DVLDGLEEVK ICVGYENTDS GCVGSSDAVS FECLKPIYET MPGWSESTVG LTSIDQLPAN ALAYVKRIEQ LIECPIDIVS TGPDRAETII LRHPFSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca aaaatgttgt ggtacttggt acccaatggg gcgacgaagg taaaggtaaa atcgtcgacc tgctcacaga tcaggcggct gcggtagtac gttatcaagg cggacataac gcaggtcata ctcttgtcgt aggtggtaaa aagactgtat tacacctcat tccatcaggc attttacgtg aaaacgtatt gtgcttaatt ggcaatggcg tggtgctttc accagcagca ttaattgaag aaatgggaat tttggaagct gagggtgttc cggttaagga gcgtctacgc atttctccaa actgtccact tattttgcct aaccacattg ctcttgatca ggcacgtgag aagaaacgtg gtaacgctaa aatcggtact acaggtcgtg gtatcggtcc tgcgtacgaa gataaagttg cgcgccgtgc agtgcgtgtt gctgaccttg ttcgtggtgg tgcagcgtta gaagaaaaac tagccgaaat gctagagtta cataacttcc aattgactca gttctacggt gtagaagcgg ttaaatttga agacgtattg gctctttgca accaatggcg tgaagttctt gctccattag tgattgacgt aactaaagta ttacatgact accgcaaaga aggtaaggct gtaatgtttg aaggtgctca aggttcactt ctagacatcg accacggtac ttatccttat gttaccagct caaatacaac tgctggtggt gtaagttctg gttcaggtat gggtccttta catcttgatt atgtattagg tattacgaaa gcttatacaa cacgtgtagg tgctggtccg ttcccaactg aattggttta cgatgctgca agtgattctg gtgacccaat tggtaaacat ttgggtactg tgggtcatga gttcggtgct tctactggtc gacaacgtcg ttgtggttgg tttgatgctg aaatcttacg ccgttctgta gatgtaaact cactttcagg tatttgctta actaaacttg acgttttaga tggtttagaa gaagtgaaaa tctgtgtggg ttatgaaaat acagattcag gttgtgtagg ttcttctgat gcggtttctt tcgaatgctt gaaaccaatt tatgaaacta tgccaggttg gagcgagtca actgttggtt taacgagcat tgaccaatta cctgcaaatg ctttggctta tgtaaaacgt attgaacaat taatcgaatg tccgattgat atcgtttcaa ctggtccaga ccgtgcagaa acaatcattt tacgtcatcc attctctgcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat