AcbaC.00867.a
putative phosphomethylpyrimidine kinase
| CENTER ID: | AcbaC.00867.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00867.a.B1.GE38663 | full length | 1 | 255 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00867.a.B1.PW37632 | full length | 1 | 255 |
Structures
4YL5
DEPOSITED: 3/5/2015
DETERMINATION: XRay
CLONE: AcbaC.00867.a.B1.GE38663
PROTEIN: AcbaC.00867.a.B1.PW37632
4YWR
DEPOSITED: 3/20/2015
DETERMINATION: XRay
CLONE: AcbaC.00867.a.B1.GE38663
PROTEIN: AcbaC.00867.a.B1.PW37632
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
Characterization
of an Acinetobacter baumannii monofunctional phosphomethylpyrimidine kinase that is
inhibited by pyridoxal phosphate
Abendroth JA, Dranow DM, Edwards TE, Lorimer D, Myler PJ, French JB, Belfon KKJ, Sharma N, De Vitto H, Toay S, Willis S, Barrera G, Beyer O, Li TD, Hicks KA, Torelli AT
Biochemistry - 2024
PMID: 36475894; PMCID: PMC9927488
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA32812.1 |
| PATRIC ID: | fig|470.1288.peg.1549 |
| PGAT: | acinetobacter:ABUW_3105 |
| RefSeq: | WP_001247938.1 |
| UniProt: | A0A059ZJ96 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VRPTVLCFSG LDPSGGAGLQ ADIEAIGQSG AHAAIACTAL TIQNSQQVFG FEATSKELLL AQANAVVGDL PIKCVKSGML GTTDNIAALA EFLRAHPDYQ YVLDPVLVAN SGGSLGDQAT LVKAFVELIP LATLITPNTV ELRALTGVTD LDQATQKLFE MGAKAVLVKG GHEDTPDFIK NSLYIDGELA ASSTCPRLEG EYHGSGCSLA SFIAGRLALG DSLKIAVQHA ETWLFGVLKN AETPVLNGQK IPKRF
NT Sequence
GTGCGTCCAA CCGTACTTTG TTTCTCGGGT TTAGACCCTT CAGGTGGTGC AGGTTTACAA GCCGATATTG AAGCGATTGG ACAAAGTGGT GCACATGCAG CAATTGCATG TACAGCCCTC ACCATTCAGA ATTCTCAACA AGTATTTGGC TTTGAAGCAA CCTCAAAAGA ACTATTATTA GCCCAAGCAA ATGCTGTTGT AGGCGATTTA CCCATTAAAT GTGTTAAGTC TGGCATGCTA GGCACTACCG ATAATATTGC GGCTCTAGCC GAATTTTTAC GTGCTCATCC TGACTATCAA TATGTACTTG ATCCTGTTTT AGTTGCAAAC AGTGGCGGTT CGTTGGGAGA CCAAGCAACT TTAGTTAAAG CATTTGTGGA GCTTATTCCT CTAGCGACAC TCATTACTCC AAATACAGTT GAATTGCGCG CTTTAACAGG CGTTACCGAC TTAGATCAAG CAACCCAAAA GTTATTTGAA ATGGGTGCCA AGGCCGTATT GGTGAAAGGT GGACATGAAG ACACACCTGA CTTTATTAAA AATAGTTTAT ATATTGATGG CGAGCTTGCA GCAAGTAGTA CATGTCCGCG CCTTGAAGGT GAATATCATG GCTCAGGTTG TTCATTAGCG AGTTTTATTG CAGGTCGTTT AGCTCTAGGT GACTCTTTAA AAATTGCAGT TCAACATGCA GAAACATGGT TATTTGGTGT GTTAAAAAAT GCTGAAACAC CTGTACTCAA TGGACAAAAG ATTCCAAAAC GGTTTTAA
Details for AcbaC.00867.a.B1.GE38663
| HARVESTED ON: | 12/9/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV RPTVLCFSGL DPSGGAGLQA DIEAIGQSGA HAAIACTALT IQNSQQVFGF EATSKELLLA QANAVVGDLP IKCVKSGMLG TTDNIAALAE FLRAHPDYQY VLDPVLVANS GGSLGDQATL VKAFVELIPL ATLITPNTVE LRALTGVTDL DQATQKLFEM GAKAVLVKGG HEDTPDFIKN SLYIDGELAA SSTCPRLEGE YHGSGCSLAS FIAGRLALGD SLKIAVQHAE TWLFGVLKNA ETPVLNGXXX PKRF
Validated NT Sequence
atcttactca cttaaaaccg ttttggaann nnnnntccat tgagtacagg tgtttcagca ttttttaaca caccaaataa ccatgtttct gcatgttgaa ctgcaatttt taaagagtca cctagagcta aacgacctgc aataaaactc gctaatgaac aacctgagcc atgatattca ccttcaaggc gcggacatgt actacttgct gcaagctcgc catcaatata taaactattt ttaataaagt caggtgtgtc ttcatgtcca cctttcacca atacggcctt ggcacccatt tcaaataact tttgggttgc ttgatctaag tcggtaacgc ctgttaaagc gcgcaattca actgtatttg gagtaatgag tgtcgctaga ggaataagct ccacaaatgc tttaactaaa gttgcttggt ctcccaacga accgccactg tttgcaacta aaacaggatc aagtacatat tgatagtcag gatgagcacg taaaaattcg gctagagccg caatattatc ggtagtgcct agcatgccag acttaacaca tttaatgggt aaatcgccta caacagcatt tgcttgggct aataatagtt cttttgaggt tgcttcaaag ccaaatactt gttgagaatt ctgaatggtg agggctgtac atgcaattgc tgcatgtgca ccactttgtc caatcgcttc aatatcggct tgtaaacctg caccacctga agggtctaaa cccgagaaac aaagtacggt tggacgcacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHVR PTVLCFSGLD PSGGAGLQAD IEAIGQSGAH AAIACTALTI QNSQQVFGFE ATSKELLLAQ ANAVVGDLPI KCVKSGMLGT TDNIAALAEF LRAHPDYQYV LDPVLVANSG GSLGDQATLV KAFVELIPLA TLITPNTVEL RALTGVTDLD QATQKLFEMG AKAVLVKGGH EDTPDFIKNS LYIDGELAAS STCPRLEGEY HGSGCSLASF IAGRLALGDS LKIAVQHAET WLFGVLKNAE TPVLNGQKIP KRF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgtc caaccgtact ttgtttctcg ggtttagacc cttcaggtgg tgcaggttta caagccgata ttgaagcgat tggacaaagt ggtgcacatg cagcaattgc atgtacagcc ctcaccattc agaattctca acaagtattt ggctttgaag caacctcaaa agaactatta ttagcccaag caaatgctgt tgtaggcgat ttacccatta aatgtgttaa gtctggcatg ctaggcacta ccgataatat tgcggctcta gccgaatttt tacgtgctca tcctgactat caatatgtac ttgatcctgt tttagttgca aacagtggcg gttcgttggg agaccaagca actttagtta aagcatttgt ggagcttatt cctctagcga cactcattac tccaaataca gttgaattgc gcgctttaac aggcgttacc gacttagatc aagcaaccca aaagttattt gaaatgggtg ccaaggccgt attggtgaaa ggtggacatg aagacacacc tgactttatt aaaaatagtt tatatattga tggcgagctt gcagcaagta gtacatgtcc gcgccttgaa ggtgaatatc atggctcagg ttgttcatta gcgagtttta ttgcaggtcg tttagctcta ggtgactctt taaaaattgc agttcaacat gcagaaacat ggttatttgg tgtgttaaaa aatgctgaaa cacctgtact caatggacaa aagattccaa aacggttttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for AcbaC.00867.a.B1.PW37632
| PURIFICATION DATe: | 1/13/2015 |
| CONCENTRATION: | 22mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV RPTVLCFSGL DPSGGAGLQA DIEAIGQSGA HAAIACTALT IQNSQQVFGF EATSKELLLA QANAVVGDLP IKCVKSGMLG TTDNIAALAE FLRAHPDYQY VLDPVLVANS GGSLGDQATL VKAFVELIPL ATLITPNTVE LRALTGVTDL DQATQKLFEM GAKAVLVKGG HEDTPDFIKN SLYIDGELAA SSTCPRLEGE YHGSGCSLAS FIAGRLALGD SLKIAVQHAE TWLFGVLKNA ETPVLNGXXX PKRF
Validated NT Sequence
atcttactca cttaaaaccg ttttggaann nnnnntccat tgagtacagg tgtttcagca ttttttaaca caccaaataa ccatgtttct gcatgttgaa ctgcaatttt taaagagtca cctagagcta aacgacctgc aataaaactc gctaatgaac aacctgagcc atgatattca ccttcaaggc gcggacatgt actacttgct gcaagctcgc catcaatata taaactattt ttaataaagt caggtgtgtc ttcatgtcca cctttcacca atacggcctt ggcacccatt tcaaataact tttgggttgc ttgatctaag tcggtaacgc ctgttaaagc gcgcaattca actgtatttg gagtaatgag tgtcgctaga ggaataagct ccacaaatgc tttaactaaa gttgcttggt ctcccaacga accgccactg tttgcaacta aaacaggatc aagtacatat tgatagtcag gatgagcacg taaaaattcg gctagagccg caatattatc ggtagtgcct agcatgccag acttaacaca tttaatgggt aaatcgccta caacagcatt tgcttgggct aataatagtt cttttgaggt tgcttcaaag ccaaatactt gttgagaatt ctgaatggtg agggctgtac atgcaattgc tgcatgtgca ccactttgtc caatcgcttc aatatcggct tgtaaacctg caccacctga agggtctaaa cccgagaaac aaagtacggt tggacgcacc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHVR PTVLCFSGLD PSGGAGLQAD IEAIGQSGAH AAIACTALTI QNSQQVFGFE ATSKELLLAQ ANAVVGDLPI KCVKSGMLGT TDNIAALAEF LRAHPDYQYV LDPVLVANSG GSLGDQATLV KAFVELIPLA TLITPNTVEL RALTGVTDLD QATQKLFEMG AKAVLVKGGH EDTPDFIKNS LYIDGELAAS STCPRLEGEY HGSGCSLASF IAGRLALGDS LKIAVQHAET WLFGVLKNAE TPVLNGQKIP KRF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCGTC CAACCGTACT TTGTTTCTCG GGTTTAGACC CTTCAGGTGG TGCAGGTTTA CAAGCCGATA TTGAAGCGAT TGGACAAAGT GGTGCACATG CAGCAATTGC ATGTACAGCC CTCACCATTC AGAATTCTCA ACAAGTATTT GGCTTTGAAG CAACCTCAAA AGAACTATTA TTAGCCCAAG CAAATGCTGT TGTAGGCGAT TTACCCATTA AATGTGTTAA GTCTGGCATG CTAGGCACTA CCGATAATAT TGCGGCTCTA GCCGAATTTT TACGTGCTCA TCCTGACTAT CAATATGTAC TTGATCCTGT TTTAGTTGCA AACAGTGGCG GTTCGTTGGG AGACCAAGCA ACTTTAGTTA AAGCATTTGT GGAGCTTATT CCTCTAGCGA CACTCATTAC TCCAAATACA GTTGAATTGC GCGCTTTAAC AGGCGTTACC GACTTAGATC AAGCAACCCA AAAGTTATTT GAAATGGGTG CCAAGGCCGT ATTGGTGAAA GGTGGACATG AAGACACACC TGACTTTATT AAAAATAGTT TATATATTGA TGGCGAGCTT GCAGCAAGTA GTACATGTCC GCGCCTTGAA GGTGAATATC ATGGCTCAGG TTGTTCATTA GCGAGTTTTA TTGCAGGTCG TTTAGCTCTA GGTGACTCTT TAAAAATTGC AGTTCAACAT GCAGAAACAT GGTTATTTGG TGTGTTAAAA AATGCTGAAA CACCTGTACT CAATGGACAA AAGATTCCAA AACGGTTTTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT