SSGCID: Target Page

AcbaC.00852.a

small GTP-binding protein

CENTER ID:	AcbaC.00852.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00852.a.B1.GE38661	full length		1	469

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3308
GenBank:	AKA33069.1
PGAT:	acinetobacter:ABUW_3373
RefSeq:	WP_000805592.1
UniProt:	V5VGI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPVIALIGR PNVGKSTLFN QITKSRDALV ADFAGLTRDR KYGDATYQNK SFIVVDTGGI GESEGGIDNY MAEQSKTAIN EADIIIFVVD ARAGLLASDE QIARELRTLG KKIYLVANKV DGVHAEAALV EFYKLGMGEP LQVAASHGRG VQQMLEDVLQ DIPEDENPEE HDKDTGLRLA IIGRPNVGKS TLVNRLLGED RVVAFDQPGT TRDSIYIPFE REGRKYTLID TAGVRRKGKV DEMIEKFSIV KTLQAMKDAH VVVVVVDARE GIVEQDLHLI GYALEAGRAM VIAINKWDNM SEYDRKQCKL DVERRFDFIP WARIHLISAL HGTGVGELYP SIHRAYESAN LKVSPAKLTQ ILNDATDQHQ PPTVQGRRIK MRYAHMGGQN PPTIVIHGNK VDKTPADYRR YLENVFRKVY KLEGTPVKIE FKTSENPFEG RKSQVDERTA ARRRRYIQKF KKAEKKFKR

NT Sequence

ATGAAACCCG TTATTGCGCT CATTGGTCGT CCGAATGTCG GAAAATCAAC GTTATTTAAC CAGATTACCA AGAGTCGTGA TGCACTTGTA GCCGACTTCG CGGGTTTGAC TCGTGACCGT AAATATGGTG ATGCGACTTA TCAAAATAAG TCTTTCATTG TTGTTGATAC TGGCGGTATT GGTGAAAGTG AAGGCGGTAT CGATAACTAC ATGGCCGAGC AGTCGAAAAC AGCCATCAAT GAAGCAGATA TTATTATTTT TGTAGTTGAT GCGCGTGCCG GATTGTTAGC TTCCGATGAG CAAATTGCCC GTGAATTACG TACTTTAGGC AAGAAAATTT ATCTTGTTGC CAATAAGGTT GATGGTGTTC ATGCTGAAGC TGCTTTGGTT GAGTTCTACA AGCTTGGTAT GGGTGAACCA TTACAAGTTG CAGCGAGCCA TGGCCGTGGC GTACAACAAA TGCTTGAAGA TGTACTTCAA GATATTCCAG AAGATGAAAA CCCTGAAGAG CATGATAAAG ATACTGGCTT ACGTCTAGCA ATTATTGGTC GTCCGAACGT AGGTAAATCA ACGTTAGTAA ATCGCTTACT TGGTGAAGAC CGTGTGGTAG CGTTTGACCA ACCGGGTACA ACTCGTGACT CTATTTATAT TCCGTTCGAG CGTGAAGGTC GTAAATACAC TTTAATTGAT ACAGCAGGTG TACGCCGTAA AGGTAAAGTT GATGAAATGA TTGAGAAATT CTCAATTGTG AAGACTTTAC AAGCGATGAA AGATGCACAT GTGGTGGTGG TTGTAGTTGA CGCCCGTGAA GGTATTGTTG AGCAAGACTT ACATTTAATT GGCTATGCGC TGGAAGCTGG GCGTGCCATG GTCATTGCGA TCAATAAATG GGACAACATG AGTGAATACG ACCGCAAGCA GTGTAAGCTT GATGTTGAGC GCCGTTTTGA CTTTATTCCT TGGGCTCGAA TTCACCTTAT TTCTGCTCTA CATGGTACTG GGGTGGGTGA GTTATATCCA TCAATTCACC GTGCTTATGA GTCTGCAAAC TTAAAGGTAT CTCCTGCGAA ATTGACGCAG ATTTTGAATG ATGCAACTGA TCAGCATCAG CCGCCGACAG TTCAAGGTCG TCGTATTAAA ATGCGTTATG CACACATGGG TGGACAAAAT CCACCGACTA TTGTGATTCA CGGTAACAAG GTGGATAAGA CTCCGGCAGA TTATCGCCGT TATTTAGAGA ATGTGTTCCG TAAGGTTTAT AAGCTTGAAG GTACACCGGT TAAAATTGAA TTTAAGACTT CTGAAAACCC GTTTGAAGGC CGTAAGAGTC AGGTCGATGA GCGTACTGCT GCACGTCGTC GTCGTTATAT TCAGAAATTC AAAAAAGCAG AAAAGAAATT TAAACGTTAA

Details for AcbaC.00852.a.B1.GE38661

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/10/2014
EXPECTED MW:	54kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK PVIALIGRPN VGKSTLFNQI TKSRDALVAD FAGLTRDRKY GDATYQNKSF IVVDTGGIGE SEGGIDNYMA EQSKTAINEA DIIIFVVDAR AGLLASDEQI ARELRTLGKK IYLVANKVDG VHAEAALVEF YKLGMGEPLQ VAASHGRGVQ QMLEDVLQDI PEDENPEEHD KDTGLRLAII GRPNVGKSTL VNRLLGEDRV VAFDQPGTTR DSIYIPFERE GRKYTLIDTA GVRRKGKVDE MIEKFSIVKT LQAMKDAHVV VVVVDAREGI VEQDLHLIGY ALEAGRAMVI AINKWDNMSE YDRKQCKLDV ERRFDFIPWA RIHLISALHG TGVGELYPSI HRAYESANLK VSPAKLTQIL NDATDQHQPP TVQGRRIKMR YAHMGGQNPP TIVIHGNKVD KTPADYRRYL ENVFRKVYKL EGTPVKIEFK TSENPFEGRK SQVDERTAAR RRRYIQKFK

Validated NT Sequence

tttttgaatt tctgaatata acgacgacga cgtgcagcag tacgctcatc gacctgactc ttacggcctt caaacgggtt ttcagaagtc ttaaattcaa ttttaaccgg tgtaccttca agcttataaa ccttacggaa cacattctct aaataacggc gataatctgc cggagtctta tccaccttgt taccgtgaat cacaatagtc ggtggatttt gtccacccat gtgtgcataa cgcattttaa tacgacgacc ttgaactgtc ggcggctgat gctgatcagt tgcatcattc aaaatctgcg tcaatttcgc aggagatacc tttaagtttg cagactcata agcacggtga attgatggat ataactcacc caccccagta ccatgtagag cagaaataag gtgaattcga gcccaaggaa taaagtcaaa acggcgctca acatcaagct tacactgctt gcggtcgtat tcactcatgt tgtcccattt attgatcgca atgaccatgg cacgcccagc ttccagcgca tagccaatta aatgtaagtc ttgctcaaca ataccttcac gggcgtcaac tacaaccacc accacatgtg catctttcat cgcttgtaaa gtcttcacaa ttgagaattt ctcaatcatt tcatcaactt tacctttacg gcgtacacct gctgtatcaa ttaaagtgta tttacgacct tcacgctcga acggaatata aatagagtca cgagttgtac ccggttggtc aaacgctacc acacggtctt caccaagtaa gcgatttact aacgttgatt tacctacgtt cggacgacca ataattgcta gacgtaagcc agtatcttta tcatgctctt cagggttttc atcttctgga atatcttgaa gtacatcttc aagcatttgt tgtacgccac ggccatggct cgctgcaact tgtaatggtt cacccatacc aagcttgtag aactcaacca aagcagcttc agcatgaaca ccatcaacct tattggcaac aagataaatt ttcttgccta aagtacgtaa ttcacgggca atttgctcat cggaagctaa caatccggca cgcgcatcaa ctacaaaaat aataatatct gcttcattga tggctgtttt cgactgctcg gccatgtagt tatcgatacc gccttcactt tcaccaatac cgccagtatc aacaacaatg aaagacttat tttgataagt cgcatcacca tatttacggt cacgagtcaa acccgcgaag tcggctacaa gtgcatcacg actcttggta atctggttaa ataacgttga ttttccgaca ttcggacgac caatgagcgc aataacgggt ttcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac ccgttattgc gctcattggt cgtccgaatg tcggaaaatc aacgttattt aaccagatta ccaagagtcg tgatgcactt gtagccgact tcgcgggttt gactcgtgac cgtaaatatg gtgatgcgac ttatcaaaat aagtctttca ttgttgttga tactggcggt attggtgaaa gtgaaggcgg tatcgataac tacatggccg agcagtcgaa aacagccatc aatgaagcag atattattat ttttgtagtt gatgcgcgtg ccggattgtt agcttccgat gagcaaattg cccgtgaatt acgtacttta ggcaagaaaa tttatcttgt tgccaataag gttgatggtg ttcatgctga agctgctttg gttgagttct acaagcttgg tatgggtgaa ccattacaag ttgcagcgag ccatggccgt ggcgtacaac aaatgcttga agatgtactt caagatattc cagaagatga aaaccctgaa gagcatgata aagatactgg cttacgtcta gcaattattg gtcgtccgaa cgtaggtaaa tcaacgttag taaatcgctt acttggtgaa gaccgtgtgg tagcgtttga ccaaccgggt acaactcgtg actctattta tattccgttc gagcgtgaag gtcgtaaata cactttaatt gatacagcag gtgtacgccg taaaggtaaa gttgatgaaa tgattgagaa attctcaatt gtgaagactt tacaagcgat gaaagatgca catgtggtgg tggttgtagt tgacgcccgt gaaggtattg ttgagcaaga cttacattta attggctatg cgctggaagc tgggcgtgcc atggtcattg cgatcaataa atgggacaac atgagtgaat acgaccgcaa gcagtgtaag cttgatgttg agcgccgttt tgactttatt ccttgggctc gaattcacct tatttctgct ctacatggta ctggggtggg tgagttatat ccatcaattc accgtgctta tgagtctgca aacttaaagg tatctcctgc gaaattgacg cagattttga atgatgcaac tgatcagcat cagccgccga cagttcaagg tcgtcgtatt aaaatgcgtt atgcacacat gggtggacaa aatccaccga ctattgtgat tcacggtaac aaggtggata agactccggc agattatcgc cgttatttag agaatgtgtt ccgtaaggtt tataagcttg aaggtacacc ggttaaaatt gaatttaaga cttctgaaaa cccgtttgaa ggccgtaaga gtcaggtcga tgagcgtact gctgcacgtc gtcgtcgtta tattcagaaa ttcaaaaaag cagaaaagaa atttaaacgt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat