SSGCID: Target Page

AcbaC.00743.a

tryptophanyl-tRNA synthetase

CENTER ID:	AcbaC.00743.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00743.a.B1.GE38659	full length		1	337

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00743.a.B1.PW37651	full length		1	337

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.849
GenBank:	AKA30641.1
PGAT:	acinetobacter:ABUW_0881
RefSeq:	WP_000072941.1
UniProt:	V5V9Z8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSNVDQRPII LTGDRPTGQL HLGHFVGSLR SRVGLQDSHH QHLLLADAQA LTDNADNPDK VRRNILEVAL DYLAVGIDPT KTTICVQSCL PALNELTMLY LNFVTVARLE RNPTIKSEIQ MRGFERDIPA GFLCYPVAQA ADITAFKATV VPVGEDQIPM IEQTNEIVRR VNRQIGQDLL PECKALLSNM ARLPGFDGKA KMSKSLGNTI VLNASDKDIK KAVNAMYTDP NHLRIEDPGQ VEGNIVFTYL DAFDPNKEEV EELKAHYRRG GLGDGTVKKR LEGVLKELIT PIRERREELA KDPDYIMDVL RQGTDKCRII TQQTLDEVKD GLGLFKF

NT Sequence

ATGAGTAATG TTGATCAGCG TCCAATTATT TTGACAGGCG ACCGCCCAAC CGGACAACTT CACCTCGGGC ATTTTGTCGG TTCTTTGCGT TCTCGTGTAG GTCTACAGGA TAGCCATCAT CAACATCTCT TATTAGCAGA TGCACAAGCG TTAACAGACA ATGCCGATAA CCCAGATAAA GTGCGCCGTA ACATTTTAGA AGTTGCTCTA GACTATTTAG CGGTAGGCAT TGATCCAACT AAAACCACAA TTTGTGTACA GTCATGCTTG CCTGCACTGA ATGAGCTTAC AATGTTATAT CTTAACTTTG TAACTGTAGC TCGATTGGAA CGTAACCCAA CCATCAAATC TGAAATTCAA ATGCGTGGTT TTGAACGTGA TATTCCTGCC GGTTTCCTTT GCTACCCTGT AGCACAGGCA GCTGATATTA CGGCTTTTAA AGCGACTGTA GTACCAGTAG GTGAAGATCA AATTCCAATG ATTGAGCAAA CTAACGAAAT CGTGCGTCGT GTAAACCGAC AAATTGGTCA AGACCTTTTA CCTGAATGTA AAGCTTTGCT TTCTAATATG GCTCGTTTAC CTGGTTTTGA TGGTAAAGCG AAAATGTCTA AGTCATTAGG CAATACCATT GTTTTAAATG CTTCTGACAA AGACATCAAA AAAGCTGTAA ATGCGATGTA TACCGATCCA AATCATCTTC GCATCGAAGA TCCGGGCCAA GTTGAAGGAA ACATTGTATT TACTTATTTA GATGCATTTG ATCCAAATAA AGAAGAAGTT GAAGAGCTAA AAGCACACTA CCGCCGTGGT GGTCTTGGTG ATGGTACAGT TAAAAAACGT CTTGAAGGTG TACTAAAAGA ATTAATTACA CCTATACGTG AACGTCGTGA AGAACTTGCT AAAGATCCTG ACTATATTAT GGATGTTTTA CGCCAAGGTA CTGATAAATG TCGAATCATT ACCCAGCAGA CTTTGGACGA AGTTAAAGAT GGTTTAGGCT TATTTAAGTT CTAA

Details for AcbaC.00743.a.B1.GE38659

HARVESTED ON:	12/9/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS NVDQRPIILT GDRPTGQLHL GHFVGSLRSR VGLQDSHHQH LLLADAQALT DNADNPDKVR RNILEVALDY LAVGIDPTKT TICVQSCLPA LNELTMLYLN FVTVARLERN PTIKSEIQMR GFERDIPAGF LCYPVAQAAD ITAFKATVVP VGEDQIPMIE QTNEIVRRVN RQIGQDLLPE CKALLSNMAR LPGFDGKAKM SKSLGNTIVL NASDKDIKKA VNAMYTDPNH LRIEDPGQVE GNIVFTYLDA FDPNKEEVEE LKAHYRRGGL GDGTVKKRLE GVLKELITPI RERREELAKD PDYIMDVLRQ GTDKCRIITQ QTLDEV

Validated NT Sequence

tttaacttcg tccaaagtct gctgggtaat gattcgacat ttatcagtac cttggcgtaa aacatccata atatagtcag gatctttagc aagttcttca cgacgttcac gtataggtgt aattaattct tttagtacac cttcaagacg ttttttaact gtaccatcac caagaccacc acggcggtag tgtgctttta gctcttcaac ttcttcttta tttggatcaa atgcatctaa ataagtaaat acaatgtttc cttcaacttg gcccggatct tcgatgcgaa gatgatttgg atcggtatac atcgcattta cagctttttt gatgtctttg tcagaagcat ttaaaacaat ggtattgcct aatgacttag acattttcgc tttaccatca aaaccaggta aacgagccat attagaaagc aaagctttac attcaggtaa aaggtcttga ccaatttgtc ggtttacacg acgcacgatt tcgttagttt gctcaatcat tggaatttga tcttcaccta ctggtactac agtcgcttta aaagccgtaa tatcagctgc ctgtgctaca gggtagcaaa ggaaaccggc aggaatatca cgttcaaaac cacgcatttg aatttcagat ttgatggttg ggttacgttc caatcgagct acagttacaa agttaagata taacattgta agctcattca gtgcaggcaa gcatgactgt acacaaattg tggttttagt tggatcaatg cctaccgcta aatagtctag agcaacttct aaaatgttac ggcgcacttt atctgggtta tcggcattgt ctgttaacgc ttgtgcatct gctaataaga gatgttgatg atggctatcc tgtagaccta cacgagaacg caaagaaccg acaaaatgcc cgaggtgaag ttgtccggtt gggcggtcgc ctgtcaaaat aattggacgc tgatcaacat tactcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta atgttgatca gcgtccaatt attttgacag gcgaccgccc aaccggacaa cttcacctcg ggcattttgt cggttctttg cgttctcgtg taggtctaca ggatagccat catcaacatc tcttattagc agatgcacaa gcgttaacag acaatgccga taacccagat aaagtgcgcc gtaacatttt agaagttgct ctagactatt tagcggtagg cattgatcca actaaaacca caatttgtgt acagtcatgc ttgcctgcac tgaatgagct tacaatgtta tatcttaact ttgtaactgt agctcgattg gaacgtaacc caaccatcaa atctgaaatt caaatgcgtg gttttgaacg tgatattcct gccggtttcc tttgctaccc tgtagcacag gcagctgata ttacggcttt taaagcgact gtagtaccag taggtgaaga tcaaattcca atgattgagc aaactaacga aatcgtgcgt cgtgtaaacc gacaaattgg tcaagacctt ttacctgaat gtaaagcttt gctttctaat atggctcgtt tacctggttt tgatggtaaa gcgaaaatgt ctaagtcatt aggcaatacc attgttttaa atgcttctga caaagacatc aaaaaagctg taaatgcgat gtataccgat ccaaatcatc ttcgcatcga agatccgggc caagttgaag gaaacattgt atttacttat ttagatgcat ttgatccaaa taaagaagaa gttgaagagc taaaagcaca ctaccgccgt ggtggtcttg gtgatggtac agttaaaaaa cgtcttgaag gtgtactaaa agaattaatt acacctatac gtgaacgtcg tgaagaactt gctaaagatc ctgactatat tatggatgtt ttacgccaag gtactgataa atgtcgaatc attacccagc agactttgga cgaagttaaa gatggtttag gcttatttaa gttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for AcbaC.00743.a.B1.PW37651

PURIFICATION DATe:	2/10/2015
CONCENTRATION:	9mg/ml
OBSERVED MW:	39kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	24
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTA ATGTTGATCA GCGTCCAATT ATTTTGACAG GCGACCGCCC AACCGGACAA CTTCACCTCG GGCATTTTGT CGGTTCTTTG CGTTCTCGTG TAGGTCTACA GGATAGCCAT CATCAACATC TCTTATTAGC AGATGCACAA GCGTTAACAG ACAATGCCGA TAACCCAGAT AAAGTGCGCC GTAACATTTT AGAAGTTGCT CTAGACTATT TAGCGGTAGG CATTGATCCA ACTAAAACCA CAATTTGTGT ACAGTCATGC TTGCCTGCAC TGAATGAGCT TACAATGTTA TATCTTAACT TTGTAACTGT AGCTCGATTG GAACGTAACC CAACCATCAA ATCTGAAATT CAAATGCGTG GTTTTGAACG TGATATTCCT GCCGGTTTCC TTTGCTACCC TGTAGCACAG GCAGCTGATA TTACGGCTTT TAAAGCGACT GTAGTACCAG TAGGTGAAGA TCAAATTCCA ATGATTGAGC AAACTAACGA AATCGTGCGT CGTGTAAACC GACAAATTGG TCAAGACCTT TTACCTGAAT GTAAAGCTTT GCTTTCTAAT ATGGCTCGTT TACCTGGTTT TGATGGTAAA GCGAAAATGT CTAAGTCATT AGGCAATACC ATTGTTTTAA ATGCTTCTGA CAAAGACATC AAAAAAGCTG TAAATGCGAT GTATACCGAT CCAAATCATC TTCGCATCGA AGATCCGGGC CAAGTTGAAG GAAACATTGT ATTTACTTAT TTAGATGCAT TTGATCCAAA TAAAGAAGAA GTTGAAGAGC TAAAAGCACA CTACCGCCGT GGTGGTCTTG GTGATGGTAC AGTTAAAAAA CGTCTTGAAG GTGTACTAAA AGAATTAATT ACACCTATAC GTGAACGTCG TGAAGAACTT GCTAAAGATC CTGACTATAT TATGGATGTT TTACGCCAAG GTACTGATAA ATGTCGAATC ATTACCCAGC AGACTTTGGA CGAAGTTAAA GATGGTTTAG GCTTATTTAA GTTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT