SSGCID: Target Page

AcbaC.00355.a

glycerol-3-phosphate dehydrogenase (NAD(P)+)

CENTER ID:	AcbaC.00355.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00355.a.B1.GE38650	full length		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00355.a.B1.PW37635	full length		1	357

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1341
GenBank:	AKA31126.1
PGAT:	acinetobacter:ABUW_1381
RefSeq:	WP_000807316.1
UniProt:	V5VBP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEFKFTDLV EPVAVDKKTA LRITVLGGGS FGTAMANLAA RNGCDTMIWI RDAETAEEIN KTHINKRYLP DFTLESSLRA VSDLEQAVCD RDIILVAIPS HSFRDVLKQI APYITAQAVV SLTKGVEAKT FSFMSDIIRE ELPEVPYGVL SGPNLAKEIM AGMPSGTVIA SDSELVRYAV QHALHSALFR VFGSDDVHGV ELGGALKNIY AVAMGIGAAY KIGENTKSMI LTRALAEMSR FAVKQGANPL TFLGLSGVGD LFATCNSPLS RNYQIGYALG SGKTLEQASK ELGQTAEGIN TIVQVRGKAQ ELDVYMPITN ALYEVIFEGA PPLNIALSLM KNGHRSDVEF VLPHHEV

NT Sequence

ATGGCTGAAT TTAAGTTTAC AGATTTGGTG GAACCTGTTG CGGTCGATAA AAAAACGGCA TTACGTATTA CCGTTTTAGG GGGCGGTAGC TTTGGTACAG CTATGGCAAA TTTAGCTGCT CGTAATGGCT GCGATACCAT GATCTGGATT CGTGATGCCG AAACAGCAGA AGAAATTAAT AAAACCCATA TTAATAAACG TTATTTACCA GACTTCACTT TAGAATCTTC ATTACGTGCA GTTTCTGATC TTGAGCAAGC GGTATGTGAT CGAGATATTA TTTTAGTTGC AATTCCAAGC CACTCATTCC GTGATGTTTT AAAACAAATA GCGCCTTATA TTACTGCACA AGCTGTGGTG TCTTTAACCA AAGGTGTTGA AGCTAAAACA TTTAGCTTCA TGAGTGATAT TATTCGTGAA GAATTACCAG AAGTGCCGTA TGGCGTATTG TCAGGACCAA ATCTCGCTAA AGAAATTATG GCAGGTATGC CTTCTGGTAC TGTAATTGCA AGTGATTCTG AACTCGTCCG TTATGCCGTA CAACATGCAC TTCATAGTGC GTTATTCCGC GTTTTTGGTA GCGATGATGT GCATGGAGTT GAGTTAGGCG GTGCGCTTAA GAATATTTAT GCCGTTGCTA TGGGAATTGG AGCAGCATAT AAAATTGGTG AAAATACCAA GAGTATGATT TTAACGCGTG CTTTGGCTGA AATGAGCCGT TTCGCGGTAA AACAAGGTGC AAACCCACTT ACGTTCTTAG GTTTATCTGG AGTAGGTGAT TTATTTGCGA CTTGTAATAG TCCATTAAGC CGTAATTATC AAATTGGTTA TGCGCTAGGT TCTGGTAAAA CGCTTGAACA AGCGAGTAAA GAATTAGGTC AAACTGCAGA AGGTATTAAC ACGATTGTTC AGGTGCGTGG CAAGGCACAA GAACTAGACG TATATATGCC AATTACCAAT GCTTTATATG AGGTCATTTT TGAAGGCGCT CCACCATTAA ATATTGCACT TTCTCTTATG AAAAATGGAC ACCGTAGTGA TGTTGAGTTT GTTTTACCTC ATCATGAAGT CTGA

Details for AcbaC.00355.a.B1.GE38650

HARVESTED ON:	12/9/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	40kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA EFKFTDLVEP VAVDKKTALR ITVLGGGSFG TAMANLAARN GCDTMIWIRD AETAEEINKT HINKRYLPDF TLESSLRAVS DLEQAVCDRD IILVAIPSHS FRDVLKQIAP YITAQAVVSL TKGVEAKTFS FMSDIIREEL PEVPYGVLSG PNLAKEIMAG MPSGTVIASD SELVRYAVQH ALHSALFRVF GSDDVHGVEL GGALKNIYAV AMGIGAAYKI GENTKSMILT RALAEMSRFA VKQGANPLTF LGLSGVGDLF ATCNSPLSRN YQIGYALGSG KTLEQASKEL GQTAEGINTI VQVRGKAQEL DVYMPITNAL YEVIFEGAPP LNIALSLMKN GHRSDVE

Validated NT Sequence

aactcaacat cactacggtg tccatttttc ataagagaaa gtgcaatatt taatggtgga gcgccttcaa aaatgacctc atataaagca ttggtaattg gcatatatac gtctagttct tgtgccttgc cacgcacctg aacaatcgtg ttaatacctt ctgcagtttg acctaattct ttactcgctt gttcaagcgt tttaccagaa cctagcgcat aaccaatttg ataattacgg cttaatggac tattacaagt cgcaaataaa tcacctactc cagataaacc taagaacgta agtgggtttg caccttgttt taccgcgaaa cggctcattt cagccaaagc acgcgttaaa atcatactct tggtattttc accaatttta tatgctgctc caattcccat agcaacggca taaatattct taagcgcacc gcctaactca actccatgca catcatcgct accaaaaacg cggaataacg cactatgaag tgcatgttgt acggcataac ggacgagttc agaatcactt gcaattacag taccagaagg catacctgcc ataatttctt tagcgagatt tggtcctgac aatacgccat acggcacttc tggtaattct tcacgaataa tatcactcat gaagctaaat gttttagctt caacaccttt ggttaaagac accacagctt gtgcagtaat ataaggcgct atttgtttta aaacatcacg gaatgagtgg cttggaattg caactaaaat aatatctcga tcacataccg cttgctcaag atcagaaact gcacgtaatg aagattctaa agtgaagtct ggtaaataac gtttattaat atgggtttta ttaatttctt ctgctgtttc ggcatcacga atccagatca tggtatcgca gccattacga gcagctaaat ttgccatagc tgtaccaaag ctaccgcccc ctaaaacggt aatacgtaat gccgtttttt tatcgaccgc aacaggttcc accaaatctg taaacttaaa ttcagccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg aatttaagtt tacagatttg gtggaacctg ttgcggtcga taaaaaaacg gcattacgta ttaccgtttt agggggcggt agctttggta cagctatggc aaatttagct gctcgtaatg gctgcgatac catgatctgg attcgtgatg ccgaaacagc agaagaaatt aataaaaccc atattaataa acgttattta ccagacttca ctttagaatc ttcattacgt gcagtttctg atcttgagca agcggtatgt gatcgagata ttattttagt tgcaattcca agccactcat tccgtgatgt tttaaaacaa atagcgcctt atattactgc acaagctgtg gtgtctttaa ccaaaggtgt tgaagctaaa acatttagct tcatgagtga tattattcgt gaagaattac cagaagtgcc gtatggcgta ttgtcaggac caaatctcgc taaagaaatt atggcaggta tgccttctgg tactgtaatt gcaagtgatt ctgaactcgt ccgttatgcc gtacaacatg cacttcatag tgcgttattc cgcgtttttg gtagcgatga tgtgcatgga gttgagttag gcggtgcgct taagaatatt tatgccgttg ctatgggaat tggagcagca tataaaattg gtgaaaatac caagagtatg attttaacgc gtgctttggc tgaaatgagc cgtttcgcgg taaaacaagg tgcaaaccca cttacgttct taggtttatc tggagtaggt gatttatttg cgacttgtaa tagtccatta agccgtaatt atcaaattgg ttatgcgcta ggttctggta aaacgcttga acaagcgagt aaagaattag gtcaaactgc agaaggtatt aacacgattg ttcaggtgcg tggcaaggca caagaactag acgtatatat gccaattacc aatgctttat atgaggtcat ttttgaaggc gctccaccat taaatattgc actttctctt atgaaaaatg gacaccgtag tgatgttgag tttgttttac ctcatcatga agtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for AcbaC.00355.a.B1.PW37635

PURIFICATION DATe:	1/20/2015
CONCENTRATION:	39mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	26
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTG AATTTAAGTT TACAGATTTG GTGGAACCTG TTGCGGTCGA TAAAAAAACG GCATTACGTA TTACCGTTTT AGGGGGCGGT AGCTTTGGTA CAGCTATGGC AAATTTAGCT GCTCGTAATG GCTGCGATAC CATGATCTGG ATTCGTGATG CCGAAACAGC AGAAGAAATT AATAAAACCC ATATTAATAA ACGTTATTTA CCAGACTTCA CTTTAGAATC TTCATTACGT GCAGTTTCTG ATCTTGAGCA AGCGGTATGT GATCGAGATA TTATTTTAGT TGCAATTCCA AGCCACTCAT TCCGTGATGT TTTAAAACAA ATAGCGCCTT ATATTACTGC ACAAGCTGTG GTGTCTTTAA CCAAAGGTGT TGAAGCTAAA ACATTTAGCT TCATGAGTGA TATTATTCGT GAAGAATTAC CAGAAGTGCC GTATGGCGTA TTGTCAGGAC CAAATCTCGC TAAAGAAATT ATGGCAGGTA TGCCTTCTGG TACTGTAATT GCAAGTGATT CTGAACTCGT CCGTTATGCC GTACAACATG CACTTCATAG TGCGTTATTC CGCGTTTTTG GTAGCGATGA TGTGCATGGA GTTGAGTTAG GCGGTGCGCT TAAGAATATT TATGCCGTTG CTATGGGAAT TGGAGCAGCA TATAAAATTG GTGAAAATAC CAAGAGTATG ATTTTAACGC GTGCTTTGGC TGAAATGAGC CGTTTCGCGG TAAAACAAGG TGCAAACCCA CTTACGTTCT TAGGTTTATC TGGAGTAGGT GATTTATTTG CGACTTGTAA TAGTCCATTA AGCCGTAATT ATCAAATTGG TTATGCGCTA GGTTCTGGTA AAACGCTTGA ACAAGCGAGT AAAGAATTAG GTCAAACTGC AGAAGGTATT AACACGATTG TTCAGGTGCG TGGCAAGGCA CAAGAACTAG ACGTATATAT GCCAATTACC AATGCTTTAT ATGAGGTCAT TTTTGAAGGC GCTCCACCAT TAAATATTGC ACTTTCTCTT ATGAAAAATG GACACCGTAG TGATGTTGAG TTTGTTTTAC CTCATCATGA AGTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT