SSGCID: Target Page

AcbaC.00249.a

thymidylate synthase

CENTER ID:	AcbaC.00249.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00249.a.B1.GE38646	full length		1	280
AcbaC.00249.a.B1.GE43935	full length		1	280

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00249.a.B1.PW38945	full length		1	280

Structures

7TA9

DEPOSITED: 12/20/2021

DETERMINATION: XRay

Diffraction Image

CLONE: AcbaC.00249.a.B1.GE43935

PROTEIN: AcbaC.00249.a.B1.PW38945

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3356
GenBank:	AKA33116.1
PGAT:	acinetobacter:ABUW_3420
RefSeq:	WP_001203170.1
UniProt:	V5VIF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRAYLDLLQH ILDNGGDKGD RTGTGTRSVF GHQMRFDLSK GFPLLTTKKV HFRSIVIELL WFLKGDTNVK YLQDNKVTIW DEWATAEQTA RFGRPEHELG PVYGHQWRNF GATKNADGTY NQDGFDQIKW LINEIKTNPN SRRLIVSGWN PNEAGQVALP PCHTLFQFFV QDNKLSCQLY QRSADVFLGV PFNIASYALL THMIAQVCGL GVGDFVWTGG DTHLYANHFE QAKLQLTREP LPLCQLKLNP EVKDIFDFKF EDIEIVGYES HPAIKAPVAV

NT Sequence

ATGCGTGCAT ATTTAGACCT TCTACAACAT ATCCTTGATA ATGGCGGTGA CAAAGGCGAC CGTACAGGCA CAGGAACCCG TTCTGTATTT GGTCATCAAA TGCGTTTTGA TCTCTCTAAG GGTTTTCCTT TACTCACCAC AAAAAAAGTT CACTTTCGTT CTATCGTGAT TGAGTTACTT TGGTTCTTAA AAGGCGATAC TAACGTTAAG TACTTACAAG ACAATAAAGT CACCATTTGG GATGAATGGG CAACAGCAGA ACAAACTGCC CGTTTCGGCC GCCCAGAACA TGAGCTTGGC CCAGTTTATG GCCATCAATG GCGTAATTTC GGCGCAACTA AAAATGCCGA TGGTACTTAT AACCAAGATG GTTTTGACCA AATTAAATGG TTAATTAATG AAATTAAAAC CAACCCAAAT TCACGCCGTT TAATAGTCTC TGGCTGGAAC CCAAATGAGG CTGGACAAGT TGCTTTACCG CCTTGCCATA CGCTCTTCCA ATTCTTCGTA CAAGACAACA AGTTGTCATG CCAGCTTTAT CAACGTAGTG CTGACGTATT CTTAGGCGTA CCATTTAATA TTGCCAGCTA TGCCCTACTC ACCCATATGA TTGCTCAAGT GTGTGGCTTA GGTGTTGGTG ATTTTGTTTG GACAGGTGGC GATACGCATC TTTATGCCAA CCATTTTGAG CAGGCAAAAC TACAATTGAC GCGTGAGCCA TTACCACTTT GCCAATTAAA ACTTAATCCA GAAGTTAAAG ATATTTTCGA CTTCAAATTT GAAGATATCG AGATTGTAGG TTACGAGTCA CATCCGGCAA TTAAAGCACC AGTTGCAGTA TAA

Details for AcbaC.00249.a.B1.GE38646

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/10/2014
EXPECTED MW:	33kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR AYLDLLQHIL DNGGDKGDRT GTGTRSVFGH QMRFDLSKGF PLLTTKKVHF RSIVIELLWF LKGDTNVKYL QDNKVTIWDE WATAEQTARF GRPEHELGPV YGHQWRNFGA TKNADGTYNQ DGFDQIKWLI NEIKTNPNSR RLIVSGWNPN EAGQVALPPC HTLFQFFVQD NKLSCQLYQR SADVFLGVPF NIASYALLTH MIAQVCGLGV GDFVWTGGDT HLYANHFEQA KLQLTREPLP LCQLKLNPEV KDIFDFKFED IEIVGYESHP AIKAPVAV

Validated NT Sequence

atggctcacc accaccacca ccatatgcgt gcatatttag accttctaca acatatcctt gataatggcg gtgacaaagg cgaccgtaca ggcacaggaa cccgttctgt atttggtcat caaatgcgtt ttgatctctc taagggtttt cctttactca ccacaaaaaa agttcacttt cgttctatcg tgattgagtt actttggttc ttaaaaggcg atactaacgt taagtactta caagacaata aagtcaccat ttgggatgaa tgggcaacag cagaacaaac tgcccgtttc ggccgcccag aacatgagct tggcccagtt tatggccatc aatggcgtaa tttcggcgca actaaaaatg ccgatggtac ttataaccaa gatggttttg accaaattaa atggttaatt aatgaaatta aaaccaaccc aaattcacgc cgtttaatag tctctggctg gaacccaaat gaggctggac aagttgcttt accgccttgc catacgctct tccaattctt cgtacaagac aacaagttgt catgccagct ttatcaacgt agtgctgacg tattcttagg cgtaccattt aatattgcca gctatgccct actcacccat atgattgctc aagtgtgtgg cttaggtgtt ggtgattttg tttggacagg tggcgatacg catctttatg ccaaccattt tgagcaggca aaactacaat tgacgcgtga gccattacca ctttgccaat taaaacttaa tccagaagtt aaagatattt tcgacttcaa atttgaagat atcgagattg taggttacga gtcacatccg gcaattaaag caccagttgc agtataagtg agtaaga

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg catatttaga ccttctacaa catatccttg ataatggcgg tgacaaaggc gaccgtacag gcacaggaac ccgttctgta tttggtcatc aaatgcgttt tgatctctct aagggttttc ctttactcac cacaaaaaaa gttcactttc gttctatcgt gattgagtta ctttggttct taaaaggcga tactaacgtt aagtacttac aagacaataa agtcaccatt tgggatgaat gggcaacagc agaacaaact gcccgtttcg gccgcccaga acatgagctt ggcccagttt atggccatca atggcgtaat ttcggcgcaa ctaaaaatgc cgatggtact tataaccaag atggttttga ccaaattaaa tggttaatta atgaaattaa aaccaaccca aattcacgcc gtttaatagt ctctggctgg aacccaaatg aggctggaca agttgcttta ccgccttgcc atacgctctt ccaattcttc gtacaagaca acaagttgtc atgccagctt tatcaacgta gtgctgacgt attcttaggc gtaccattta atattgccag ctatgcccta ctcacccata tgattgctca agtgtgtggc ttaggtgttg gtgattttgt ttggacaggt ggcgatacgc atctttatgc caaccatttt gagcaggcaa aactacaatt gacgcgtgag ccattaccac tttgccaatt aaaacttaat ccagaagtta aagatatttt cgacttcaaa tttgaagata tcgagattgt aggttacgag tcacatccgg caattaaagc accagttgca gtatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for AcbaC.00249.a.B1.GE43935

HARVESTED ON:	12/3/2020
SEQUENCED ON:	12/16/2020
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

Details for AcbaC.00249.a.B1.PW38945

PURIFICATION DATe:	2/24/2021
CONCENTRATION:	34mg/ml
OBSERVED MW:	32kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

runs small on SDS-PAGE, was a requeue and did not behave the same on SEC the second go around

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTG CATATTTAGA CCTTCTACAA CATATCCTTG ATAATGGCGG TGACAAAGGC GACCGTACAG GCACAGGAAC CCGTTCTGTA TTTGGTCATC AAATGCGTTT TGATCTCTCT AAGGGTTTTC CTTTACTCAC CACAAAAAAA GTTCACTTTC GTTCTATCGT GATTGAGTTA CTTTGGTTCT TAAAAGGCGA TACTAACGTT AAGTACTTAC AAGACAATAA AGTCACCATT TGGGATGAAT GGGCAACAGC AGAACAAACT GCCCGTTTCG GCCGCCCAGA ACATGAGCTT GGCCCAGTTT ATGGCCATCA ATGGCGTAAT TTCGGCGCAA CTAAAAATGC CGATGGTACT TATAACCAAG ATGGTTTTGA CCAAATTAAA TGGTTAATTA ATGAAATTAA AACCAACCCA AATTCACGCC GTTTAATAGT CTCTGGCTGG AACCCAAATG AGGCTGGACA AGTTGCTTTA CCGCCTTGCC ATACGCTCTT CCAATTCTTC GTACAAGACA ACAAGTTGTC ATGCCAGCTT TATCAACGTA GTGCTGACGT ATTCTTAGGC GTACCATTTA ATATTGCCAG CTATGCCCTA CTCACCCATA TGATTGCTCA AGTGTGTGGC TTAGGTGTTG GTGATTTTGT TTGGACAGGT GGCGATACGC ATCTTTATGC CAACCATTTT GAGCAGGCAA AACTACAATT GACGCGTGAG CCATTACCAC TTTGCCAATT AAAACTTAAT CCAGAAGTTA AAGATATTTT CGACTTCAAA TTTGAAGATA TCGAGATTGT AGGTTACGAG TCACATCCGG CAATTAAAGC ACCAGTTGCA GTATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT