AcbaC.00206.a

phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase

CENTER ID: AcbaC.00206.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00206.a.B1.GE38644 full length 1 417
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1288.peg.2146
GenBank: AKA30301.1
PGAT: acinetobacter:ABUW_0529
RefSeq: WP_000006958.1
UniProt: V5V9S5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VSFDLSVIPH KNIVLAVTGG IAAYKSAILV RRLKDYGFDV RVVMTHGAQA FITPLTFQAL SGNPVHTELL DPEAEAGMGH IELARWADLL LVAPASCDTL AKFANGLADD LLSTLFLATK APVWVAPAMN QQMWAAKATQ RNLQTLVEDG VHVIMPDAGE QACGDVGLGR MPEPEEIARQ VAGYFHQAQR AIAEKFGLLA GKRVVITAGP TREAIDPVRY ISNHSTGKMG FALAAACYAA GAKVTLIAGP VSLDTPNGVQ RVNVASARQM LDVSMESLET GCDIFIATAA VADYRVAEVA EHKIKKAGDE LNVSLVKNPD IVATIAAQEK RPFMVGFAAE TQNVEEYAAG KLVAKKLDMI ACNDVSRADI GFASDENAMT VFFAESYHMK KRELEKASKQ EISQQLVEAI SDALRRK
NT Sequence
GTGAGCTTTG ATTTAAGTGT TATTCCTCAT AAAAACATTG TTTTAGCTGT TACAGGTGGG ATTGCTGCCT ATAAGAGTGC CATTTTGGTC CGTCGTCTTA AAGATTATGG TTTTGACGTT CGGGTTGTGA TGACACACGG GGCTCAAGCA TTTATTACAC CTCTAACTTT TCAGGCTCTT TCAGGAAATC CTGTACATAC CGAGCTTTTA GACCCTGAGG CTGAAGCTGG AATGGGCCAT ATTGAATTGG CTCGCTGGGC TGATTTATTA CTGGTTGCTC CAGCAAGTTG TGACACATTA GCTAAGTTTG CTAATGGTTT GGCAGATGAC TTACTCAGTA CTTTATTTTT AGCAACTAAA GCGCCTGTGT GGGTCGCTCC TGCGATGAAC CAGCAAATGT GGGCTGCAAA AGCAACTCAG CGTAATTTGC AAACTCTGGT TGAAGATGGC GTGCATGTGA TTATGCCAGA TGCAGGGGAG CAGGCGTGTG GTGATGTAGG CTTAGGCCGT ATGCCGGAAC CAGAAGAAAT TGCAAGGCAA GTGGCGGGTT ATTTCCATCA AGCCCAACGT GCAATTGCTG AAAAATTTGG GTTGTTGGCA GGTAAGCGAG TTGTAATTAC TGCTGGACCA ACCCGTGAAG CGATTGACCC TGTTCGTTAT ATCTCAAATC ATAGTACTGG AAAAATGGGC TTTGCTTTAG CTGCGGCATG TTATGCAGCA GGCGCAAAAG TTACGTTAAT TGCTGGTCCC GTAAGTTTAG ATACTCCAAA CGGTGTTCAA CGTGTAAATG TTGCTTCGGC TCGACAAATG TTAGATGTCA GTATGGAGTC GCTTGAGACT GGCTGTGACA TCTTTATTGC GACAGCGGCT GTAGCTGATT ATCGCGTTGC CGAAGTGGCA GAGCATAAAA TTAAGAAAGC AGGGGATGAG CTGAATGTTT CCCTTGTAAA AAATCCGGAT ATTGTGGCAA CGATTGCAGC TCAGGAAAAA CGTCCATTTA TGGTAGGCTT TGCTGCCGAA ACGCAGAATG TTGAAGAATA TGCAGCAGGG AAACTCGTTG CTAAAAAGCT AGATATGATT GCATGTAACG ATGTTTCTCG TGCTGATATT GGCTTTGCTT CTGATGAAAA TGCGATGACA GTATTTTTTG CTGAAAGTTA CCATATGAAA AAACGTGAGT TAGAAAAAGC ATCTAAGCAA GAAATTTCTC AACAATTGGT TGAAGCTATT TCGGATGCAT TACGCCGTAA ATAA
Details for AcbaC.00206.a.B1.GE38644
HARVESTED ON: 12/9/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV SFDLSVIPHK NIVLAVTGGI AAYKSAILVR RLKDYGFDVR VVMTHGAQAF ITPLTFQALS GNPVHTELLD PEAEAGMGHI ELARWADLLL VAPASCDTLA KFANGLADDL LSTLFLATKA PVWVAPAMNQ QMWAAKATQR NLQTLVEDGV HVIMPDAGEQ ACGDVGLGRM PEPEEIARQV AGYFHQAQRA IAEKFGLLAG KRVVITAGPT REAIDPVRYI SNHSTGKMGF ALAAACYAAG AKVTLIAGPV SLDTPNGVQR VNVASARQML DVSMESLETG CDIFIATAAV ADYRVAEVAE HKIKKAGDEL NVSLVKNPDI VATIAAQEKR PFMVGFAAET QNVEEYAAGK LVAKKLDMIA CNDVSRADIG FASDENAMTV FFAESYHMKK RELEKASKQE ISQQLVEA
Validated NT Sequence
tagcttcaac caattgttga gaaatttctt gcttagatgc tttttctaac tcacgttttt tcatatggta actttcagca aaaaatactg tcatcgcatt ttcatcagaa gcaaagccaa tatcagcacg agaaacatcg ttacatgcaa tcatatctag ctttttagca acgagtttcc ctgctgcata ttcttcaaca ttctgcgttt cggcagcaaa gcctaccata aatggacgtt tttcctgagc tgcaatcgtt gccacaatat ccggattttt tacaagggaa acattcagct catcccctgc tttcttaatt ttatgctctg ccacttcggc aacgcgataa tcagctacag ccgctgtcgc aataaagatg tcacagccag tctcaagcga ctccatactg acatctaaca tttgtcgagc cgaagcaaca tttacacgtt gaacaccgtt tggagtatct aaacttacgg gaccagcaat taacgtaact tttgcgcctg ctgcataaca tgccgcagct aaagcaaagc ccatttttcc agtactatga tttgagatat aacgaacagg gtcaatcgct tcacgggttg gtccagcagt aattacaact cgcttacctg ccaacaaccc aaatttttca gcaattgcac gttgggcttg atggaaataa cccgccactt gccttgcaat ttcttctggt tccggcatac ggcctaagcc tacatcacca cacgcctgct cccctgcatc tggcataatc acatgcacgc catcttcaac cagagtttgc aaattacgct gagttgcttt tgcagcccac atttgctggt tcatcgcagg agcgacccac acaggcgctt tagttgctaa aaataaagta ctgagtaagt catctgccaa accattagca aacttagcta atgtgtcaca acttgctgga gcaaccagta ataaatcagc ccagcgagcc aattcaatat ggcccattcc agcttcagcc tcagggtcta aaagctcggt atgtacagga tttcctgaaa gagcctgaaa agttagaggt gtaataaatg cttgagcccc gtgtgtcatc acaacccgaa cgtcaaaacc ataatcttta agacgacgga ccaaaatggc actcttatag gcagcaatcc cacctgtaac agctaaaaca atgtttttat gaggaataac acttaaatca aagctcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHVS FDLSVIPHKN IVLAVTGGIA AYKSAILVRR LKDYGFDVRV VMTHGAQAFI TPLTFQALSG NPVHTELLDP EAEAGMGHIE LARWADLLLV APASCDTLAK FANGLADDLL STLFLATKAP VWVAPAMNQQ MWAAKATQRN LQTLVEDGVH VIMPDAGEQA CGDVGLGRMP EPEEIARQVA GYFHQAQRAI AEKFGLLAGK RVVITAGPTR EAIDPVRYIS NHSTGKMGFA LAAACYAAGA KVTLIAGPVS LDTPNGVQRV NVASARQMLD VSMESLETGC DIFIATAAVA DYRVAEVAEH KIKKAGDELN VSLVKNPDIV ATIAAQEKRP FMVGFAAETQ NVEEYAAGKL VAKKLDMIAC NDVSRADIGF ASDENAMTVF FAESYHMKKR ELEKASKQEI SQQLVEAISD ALRRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagct ttgatttaag tgttattcct cataaaaaca ttgttttagc tgttacaggt gggattgctg cctataagag tgccattttg gtccgtcgtc ttaaagatta tggttttgac gttcgggttg tgatgacaca cggggctcaa gcatttatta cacctctaac ttttcaggct ctttcaggaa atcctgtaca taccgagctt ttagaccctg aggctgaagc tggaatgggc catattgaat tggctcgctg ggctgattta ttactggttg ctccagcaag ttgtgacaca ttagctaagt ttgctaatgg tttggcagat gacttactca gtactttatt tttagcaact aaagcgcctg tgtgggtcgc tcctgcgatg aaccagcaaa tgtgggctgc aaaagcaact cagcgtaatt tgcaaactct ggttgaagat ggcgtgcatg tgattatgcc agatgcaggg gagcaggcgt gtggtgatgt aggcttaggc cgtatgccgg aaccagaaga aattgcaagg caagtggcgg gttatttcca tcaagcccaa cgtgcaattg ctgaaaaatt tgggttgttg gcaggtaagc gagttgtaat tactgctgga ccaacccgtg aagcgattga ccctgttcgt tatatctcaa atcatagtac tggaaaaatg ggctttgctt tagctgcggc atgttatgca gcaggcgcaa aagttacgtt aattgctggt cccgtaagtt tagatactcc aaacggtgtt caacgtgtaa atgttgcttc ggctcgacaa atgttagatg tcagtatgga gtcgcttgag actggctgtg acatctttat tgcgacagcg gctgtagctg attatcgcgt tgccgaagtg gcagagcata aaattaagaa agcaggggat gagctgaatg tttcccttgt aaaaaatccg gatattgtgg caacgattgc agctcaggaa aaacgtccat ttatggtagg ctttgctgcc gaaacgcaga atgttgaaga atatgcagca gggaaactcg ttgctaaaaa gctagatatg attgcatgta acgatgtttc tcgtgctgat attggctttg cttctgatga aaatgcgatg acagtatttt ttgctgaaag ttaccatatg aaaaaacgtg agttagaaaa agcatctaag caagaaattt ctcaacaatt ggttgaagct atttcggatg cattacgccg taaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat