AcbaC.00189.a
dihydrodipicolinate reductase
| CENTER ID: | AcbaC.00189.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00189.a.B1.GE43934 | full length | 1 | 273 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
8D57
DEPOSITED: 6/4/2022
DETERMINATION: XRay
CLONE: AcbaC.00189.a.B1.GE43934
PROTEIN: AcbaC.00189.a.B1.PW38917
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|470.1345.peg.35 |
| GenBank: | AKA29834.1 |
| PGAT: | acinetobacter:ABUW_0039 |
| RefSeq: | WP_001271382.1 |
| UniProt: | A0A0D5YCZ0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAAPRIGIL GAGGRMGRIL IQAVQQAGYQ LGAAVVRPES TLIGADAGEL AGIGSIGVKL TGSLAEVLED CDVVIDFSTP AATSEHLKLC REAGVAIVIG TTGMSDEQKA ELDETAKHIP VVYAANYSVG VNVSIKLLEL AAKVFGDTVD IEVIEAHHRH KVDAPSGTAL MMGEAIADTL GRNLKEVAVY GREGHTGPRD RQTIGFETIR GGDIVGEHTV MFIGEGERVE VTHKATNRMN FAAGAVRAAA WVVGREARKY DMKDVLGLND VQV
NT Sequence
ATGTCAGCAG CTCCACGCAT TGGTATTTTG GGTGCAGGCG GCCGTATGGG TCGTATACTT ATTCAGGCAG TTCAACAAGC AGGTTATCAA TTGGGAGCCG CTGTTGTACG CCCAGAAAGT ACTTTAATCG GTGCTGATGC TGGTGAACTT GCAGGAATTG GTTCAATTGG AGTGAAGCTT ACCGGAAGCT TGGCAGAGGT TCTTGAAGAC TGTGATGTTG TAATTGACTT TTCGACTCCT GCAGCAACAT CTGAGCATTT AAAGCTATGT CGTGAAGCTG GTGTAGCTAT AGTGATTGGG ACAACGGGAA TGTCTGATGA ACAAAAAGCT GAGCTTGATG AAACGGCAAA ACATATACCT GTTGTATATG CTGCGAACTA CTCTGTTGGC GTAAATGTAT CAATCAAGTT ACTTGAGCTT GCAGCAAAAG TATTTGGCGA TACAGTAGAT ATTGAAGTGA TTGAGGCTCA TCACCGTCAT AAAGTAGATG CACCATCTGG TACTGCATTA ATGATGGGTG AAGCAATTGC GGATACGTTA GGCCGTAACT TAAAAGAAGT TGCTGTTTAT GGTCGTGAAG GGCATACCGG TCCACGTGAT CGTCAAACCA TTGGTTTCGA AACCATCCGT GGTGGCGATA TTGTAGGGGA ACATACGGTC ATGTTTATTG GTGAGGGTGA GCGTGTTGAA GTGACTCACA AAGCCACTAA CCGTATGAAC TTTGCTGCCG GTGCAGTACG AGCTGCTGCA TGGGTTGTGG GCCGTGAAGC GCGTAAATAT GACATGAAAG ATGTTTTAGG GTTGAACGAC GTACAGGTGT AA
Details for AcbaC.00189.a.B1.GE43934
| HARVESTED ON: | 12/3/2020 |
| SEQUENCED ON: | 12/16/2020 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 22 |
| PERCENT COVERAGE: | 109 |
Validated AA Sequence
MAHHHHHHMS AAPRIAVQQI DPHELKAWIK AQALDLGFAD CVIAKPDAQE QMPRFLEYLE RGYHADMTYL EENLEKRADP TLLVPGTKSI ICVRMNYLVE SPKPRYVPFE PNSAIIARYA RGRDYHKVMR GRLKTLATRI REKVGDFESR PFADSAPIFE KSLAESAGMG WTGKHTLLIH KKSGSFFVLG ELFTSLDLPF DEPATSHCGS CSACIDICPT QAIVEPYMLD ARRCIAYLTI EYKGIIAEEL RAGIGNRIFG CDDCQLICPW NSFAKTASIP DFNPRHGLDN ISLLDIWQWD GATFLANTE
Validated NT Sequence
atggctcacc accaccacca ccatatgtca gcagctccac gcattgcagt gcaacaaatt gatccacatg aattaaaagc atggatcaaa gctcaagcat tagacttggg ttttgctgat tgtgtcattg ccaaaccaga tgctcaagag caaatgccac gttttttaga ataccttgaa cgtggttatc atgcagatat gacctattta gaagaaaatc tggaaaagcg agcagatcca actttattgg tgcctggaac aaaaagtatt atttgcgtac gtatgaatta tttggtggaa tcgccaaaac cacgatatgt acctttcgaa ccaaactctg cgattattgc ccgttatgct cgtggtcgtg attaccataa ggtgatgcgt ggcagattaa aaacgttagc cacacgtatc cgtgaaaaag tgggagattt tgaatctcgc ccttttgcag actcggcacc tatttttgag aaatcattag ctgaaagtgc tgggatgggc tggacaggta aacatacttt actcattcat aaaaagtcag gttcattctt tgtcttaggt gaactattta catcacttga cttacctttt gatgaacctg ctacttctca ctgcggttct tgtagtgcgt gtattgatat ttgcccgaca caagcgattg tcgaacctta tatgttagat gctagacgct gtatcgctta tctcaccatc gaatataaag gaatcattgc agaagaatta cgtgcaggca ttggtaaccg gatatttggt tgtgatgact gccaattaat ttgcccgtgg aatagttttg ctaaaacagc gtctattccc gatttcaatc ctagacatgg tttagataat attagtctgc ttgatatttg gcagtgggac ggagctactt ttttagccaa tacagaaggc
Expected Protein Sequence
MAHHHHHHMS AAPRIGILGA GGRMGRILIQ AVQQAGYQLG AAVVRPESTL IGADAGELAG IGSIGVKLTG SLAEVLEDCD VVIDFSTPAA TSEHLKLCRE AGVAIVIGTT GMSDEQKAEL DETAKHIPVV YAANYSVGVN VSIKLLELAA KVFGDTVDIE VIEAHHRHKV DAPSGTALMM GEAIADTLGR NLKEVAVYGR EGHTGPRDRQ TIGFETIRGG DIVGEHTVMF IGEGERVEVT HKATNRMNFA AGAVRAAAWV VGREARKYDM KDVLGLNDVQ V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag cagctccacg cattggtatt ttgggtgcag gcggccgtat gggtcgtata cttattcagg cagttcaaca agcaggttat caattgggag ccgctgttgt acgcccagaa agtactttaa tcggtgctga tgctggtgaa cttgcaggaa ttggttcaat tggagtgaag cttaccggaa gcttggcaga ggttcttgaa gactgtgatg ttgtaattga cttttcgact cctgcagcaa catctgagca tttaaagcta tgtcgtgaag ctggtgtagc tatagtgatt gggacaacgg gaatgtctga tgaacaaaaa gctgagcttg atgaaacggc aaaacatata cctgttgtat atgctgcgaa ctactctgtt ggcgtaaatg tatcaatcaa gttacttgag cttgcagcaa aagtatttgg cgatacagta gatattgaag tgattgaggc tcatcaccgt cataaagtag atgcaccatc tggtactgca ttaatgatgg gtgaagcaat tgcggatacg ttaggccgta acttaaaaga agttgctgtt tatggtcgtg aagggcatac cggtccacgt gatcgtcaaa ccattggttt cgaaaccatc cgtggtggcg atattgtagg ggaacatacg gtcatgttta ttggtgaggg tgagcgtgtt gaagtgactc acaaagccac taaccgtatg aactttgctg ccggtgcagt acgagctgct gcatgggttg tgggccgtga agcgcgtaaa tatgacatga aagatgtttt agggttgaac gacgtacagg tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat