AcbaC.00177.a

Fe-S protein assembly chaperone HscA

CENTER ID: AcbaC.00177.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00177.a.B1.GE38606 full length 1 619
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2161
GenBank: AKA31934.1
PGAT: acinetobacter:ABUW_2206
RefSeq: WP_001196578.1
UniProt: A0A0D5YJ26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALLQIAEPG QSSAPHQHRI AIGIDLGTTH SLVATVLSGK PKVLNDVQNR RLLPSIVHYG DNTTHYGEEA KPFIIADPKN TIVSVKRFMG RSKADIKFQH PYELVGSENE MPAFETRAGR KTPVEISAEI LKQLKDRAED SLQNPVNGAV ITVPAYFDEA QRQATRDAAQ LAGLNVLRLL NEPTAAAVAY GLDQESNLAT DRNYVIYDLG GGTFDVSILR FSQGVFEVLA TGGHTALGGD DLDRLIVKWA KKQLNIDVLS DEDYAVFIVA ARQAKEQLST QDSVELKLLE ATLTLDRPTF ESIIQVALDK TISVCKRVLR DAKLELTDIQ NVVLVGGSTR SYAVQKAVRE VFAQEPLCTI NPDEVVAIGA SITANQLIGN SQDGSLLLDV TPLSLGLETM GGLVERLISR NTAIPVARRQ EFTTYQDGQT AMLIHVVQGE RDLVEHCRSL GRFVLHGIPP MTAGQARIEV TFQVDADGLL TVSAREATSG VQAHIDIKPS YGLSEADTER LLIEGFQHAE EDKNLRHLKE TKVEAERELE SLEQALKVDA DLLDEKQLDA LNSAKESLKA QLEGSDIQAI EHAVQQLKVH SDAFAALRMN RHIDHALKGT KLDDWSKSN
NT Sequence
ATGGCACTTT TGCAAATTGC TGAACCGGGT CAATCCAGTG CCCCACACCA ACATCGCATT GCGATTGGTA TTGACCTAGG AACAACACAC TCATTAGTTG CGACAGTGTT ATCAGGCAAA CCTAAAGTAC TCAATGATGT TCAAAATAGA AGATTACTTC CTTCTATCGT TCATTATGGG GACAATACAA CTCATTATGG TGAAGAAGCT AAACCGTTTA TCATTGCAGA TCCAAAAAAT ACAATTGTTT CTGTTAAACG ATTTATGGGC CGTTCAAAGG CAGATATTAA ATTTCAACAC CCTTATGAGT TAGTAGGCTC AGAAAATGAA ATGCCTGCTT TTGAAACCCG TGCTGGACGC AAAACACCAG TAGAAATCTC AGCTGAAATT TTAAAACAAT TAAAAGATCG CGCTGAAGAC AGCTTACAAA ATCCTGTTAA TGGAGCAGTA ATTACCGTTC CTGCATATTT CGATGAAGCT CAGCGTCAAG CAACTCGCGA TGCAGCTCAA CTTGCAGGTT TAAATGTATT ACGTTTATTA AATGAGCCTA CAGCTGCCGC TGTAGCTTAT GGATTAGATC AAGAAAGTAA TTTAGCGACC GACCGTAATT ATGTAATTTA TGACTTGGGT GGCGGTACTT TTGACGTATC TATCTTACGT TTTTCACAAG GCGTATTCGA AGTTTTAGCG ACTGGTGGTC ACACTGCTTT AGGTGGTGAT GACTTAGACC GTCTAATCGT AAAATGGGCT AAAAAACAAC TTAATATTGA TGTATTAAGT GATGAAGATT ATGCCGTATT TATTGTGGCA GCACGTCAAG CTAAAGAACA ATTATCAACT CAAGATTCAG TTGAATTAAA GTTACTCGAA GCAACTCTCA CACTAGATCG CCCTACATTT GAAAGCATTA TTCAAGTAGC ATTAGACAAA ACGATTAGTG TTTGTAAGCG TGTACTGCGT GATGCAAAAC TCGAGTTAAC CGATATTCAA AATGTTGTTT TGGTAGGTGG TTCTACTCGC TCTTATGCTG TTCAAAAAGC AGTACGGGAA GTTTTTGCCC AAGAACCTCT TTGCACGATC AACCCAGATG AAGTGGTGGC AATTGGCGCT TCTATTACAG CCAACCAGTT AATCGGTAAT AGCCAAGATG GCTCTCTTCT TTTAGATGTA ACTCCTCTTT CTCTTGGTCT AGAGACTATG GGAGGACTCG TTGAGCGCCT GATATCTCGT AATACAGCGA TTCCTGTTGC ACGTCGTCAA GAGTTCACCA CTTATCAAGA TGGTCAAACT GCTATGTTAA TTCATGTAGT TCAAGGCGAA CGTGATTTAG TTGAACATTG CCGTTCATTA GGTAGATTTG TACTTCATGG CATTCCACCA ATGACTGCAG GCCAAGCTCG TATTGAAGTC ACTTTCCAAG TTGATGCAGA TGGCTTACTC ACCGTTTCTG CTCGTGAAGC AACTTCTGGC GTGCAAGCTC ATATTGATAT CAAACCATCT TATGGTTTAT CTGAGGCAGA TACGGAACGC TTATTAATTG AAGGTTTCCA ACATGCCGAA GAGGACAAAA ATCTTCGTCA TTTAAAAGAA ACTAAAGTTG AAGCAGAACG TGAGCTTGAG TCTCTTGAGC AAGCATTAAA AGTAGATGCG GATTTACTTG ATGAAAAGCA GCTTGACGCA CTCAACTCTG CTAAGGAATC ATTAAAAGCT CAGCTTGAAG GCAGTGATAT TCAAGCAATT GAGCACGCAG TTCAACAACT TAAAGTACAT AGTGATGCTT TTGCAGCACT ACGGATGAAT CGACATATTG ACCATGCCTT AAAAGGCACG AAGCTCGATG ATTGGTCAAA ATCAAATTAA
Details for AcbaC.00177.a.B1.GE38606
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA LLQIAEPGQS SAPHQHRIAI GIDLGTTHSL VATVLSGKPK VLNDVQNRRL LPSIVHYGDN TTHYGEEAKP FIIADPKNTI VSVKRFMGRS KADIKFQHPY ELVGSENEMP AFETRAGRKT PVEISAEILK QLKDRAEDSL QNPVNGAVIT VPAYFDEAQR QATRDAAQLA GLNVLRLLNE PTAAAVAYGL DQESNLATDR NYVIYDLGGG TFDVSILRFS QGVFEVLATG GHTALGGDDL DRLIVKWAKK QLNIDVLSDE DYAVFIVAAR QAKEQLSTQD SVELKLLEAT LTLDRPTFES IIQVALDKTI SVCKRVLRDA KLELTDIQNV VLVGGSTRSY AVQKAVREVF AQEPLCTINP DEVVAIGASI TANQLIGNSQ DGSLLLDVTP LSLGLETMGG LVERLISRNT AIPVARRQEF TTYQDGQTAM LIHVVQGERD LVEHCRSLGR FVLHGIPPMT AGQARIEVTF QVDADGLLTV SAREATSGVQ AHIDIKPSYG LSEADTERLL IEGFQHAEED KNLRHLKETK VEAERELESL EQALKVDADL LDEKQLDALN SAKESLKAQL EGSDIQAIEH AVQQLKVHSD AFAALRMNRH IDHALKGTKL DDWSKSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac ttttgcaaat tgctgaaccg ggtcaatcca gtgccccaca ccaacatcgc attgcgattg gtattgacct aggaacaaca cactcattag ttgcgacagt gttatcaggc aaacctaaag tactcaatga tgttcaaaat agaagattac ttccttctat cgttcattat ggggacaata caactcatta tggtgaagaa gctaaaccgt ttatcattgc agatccaaaa aatacaattg tttctgttaa acgatttatg ggccgttcaa aggcagatat taaatttcaa cacccttatg agttagtagg ctcagaaaat gaaatgcctg cttttgaaac ccgtgctgga cgcaaaacac cagtagaaat ctcagctgaa attttaaaac aattaaaaga tcgcgctgaa gacagcttac aaaatcctgt taatggagca gtaattaccg ttcctgcata tttcgatgaa gctcagcgtc aagcaactcg cgatgcagct caacttgcag gtttaaatgt attacgttta ttaaatgagc ctacagctgc cgctgtagct tatggattag atcaagaaag taatttagcg accgaccgta attatgtaat ttatgacttg ggtggcggta cttttgacgt atctatctta cgtttttcac aaggcgtatt cgaagtttta gcgactggtg gtcacactgc tttaggtggt gatgacttag accgtctaat cgtaaaatgg gctaaaaaac aacttaatat tgatgtatta agtgatgaag attatgccgt atttattgtg gcagcacgtc aagctaaaga acaattatca actcaagatt cagttgaatt aaagttactc gaagcaactc tcacactaga tcgccctaca tttgaaagca ttattcaagt agcattagac aaaacgatta gtgtttgtaa gcgtgtactg cgtgatgcaa aactcgagtt aaccgatatt caaaatgttg ttttggtagg tggttctact cgctcttatg ctgttcaaaa agcagtacgg gaagtttttg cccaagaacc tctttgcacg atcaacccag atgaagtggt ggcaattggc gcttctatta cagccaacca gttaatcggt aatagccaag atggctctct tcttttagat gtaactcctc tttctcttgg tctagagact atgggaggac tcgttgagcg cctgatatct cgtaatacag cgattcctgt tgcacgtcgt caagagttca ccacttatca agatggtcaa actgctatgt taattcatgt agttcaaggc gaacgtgatt tagttgaaca ttgccgttca ttaggtagat ttgtacttca tggcattcca ccaatgactg caggccaagc tcgtattgaa gtcactttcc aagttgatgc agatggctta ctcaccgttt ctgctcgtga agcaacttct ggcgtgcaag ctcatattga tatcaaacca tcttatggtt tatctgaggc agatacggaa cgcttattaa ttgaaggttt ccaacatgcc gaagaggaca aaaatcttcg tcatttaaaa gaaactaaag ttgaagcaga acgtgagctt gagtctcttg agcaagcatt aaaagtagat gcggatttac ttgatgaaaa gcagcttgac gcactcaact ctgctaagga atcattaaaa gctcagcttg aaggcagtga tattcaagca attgagcacg cagttcaaca acttaaagta catagtgatg cttttgcagc actacggatg aatcgacata ttgaccatgc cttaaaaggc acgaagctcg atgattggtc aaaatcaaat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat