AcbaC.00170.a
NADH-dependent enoyl-ACP reductase
| CENTER ID: | AcbaC.00170.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00170.a.B1.GE38605 | full length | 1 | 267 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00170.a.B1.PW37673 | full length | 1 | 267 |
Structures
4ZJU
DEPOSITED: 4/29/2015
DETERMINATION: XRay
CLONE: AcbaC.00170.a.B1.GE38605
PROTEIN: AcbaC.00170.a.B1.PW37673
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA33061.1 |
| PATRIC ID: | fig|470.1345.peg.3300 |
| PGAT: | acinetobacter:ABUW_3365 |
| RefSeq: | WP_002027450.1 |
| UniProt: | A0A077GA87 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQGLLAGKR FLIAGVASKL SIAYGIAQAL HREGAELAFT YPNEKLKKRV DEFAEQFGSK LVFPCDVAVD AEIDNAFAEL AKHWDGVDGV VHSIGFAPAH TLDGDFTDVT DRDGFKIAHD ISAYSFVAMA RAAKPLLQAR QGCLLTLTYQ GSERVMPNYN VMGMAKASLE AGVRYLASSL GVDGIRVNAI SAGPIRTLAA SGIKSFRKML DANEKVAPLK RNVTIEEVGN AALFLCSPWA SGITGEILYV DAGFNTVGMS QSMMDDE
NT Sequence
ATGACACAAG GACTTTTAGC AGGTAAACGC TTTTTGATTG CAGGCGTTGC TAGTAAATTA TCAATTGCTT ATGGTATTGC ACAAGCATTA CACCGTGAAG GTGCTGAGCT TGCATTTACT TATCCAAACG AAAAATTAAA AAAACGTGTA GATGAGTTTG CTGAGCAATT CGGTTCTAAG CTTGTGTTTC CATGTGATGT TGCCGTTGAT GCTGAAATTG ATAATGCATT TGCGGAACTT GCAAAACATT GGGACGGCGT AGATGGTGTT GTGCACTCAA TCGGCTTTGC ACCTGCACAC ACGCTTGATG GTGACTTCAC TGACGTGACT GACCGTGACG GTTTTAAAAT TGCTCATGAC ATTAGCGCAT ACAGCTTTGT TGCAATGGCA CGTGCTGCAA AACCTTTGTT ACAAGCTCGC CAAGGTTGTT TATTAACTTT GACTTACCAA GGTTCTGAGC GCGTTATGCC TAACTACAAC GTAATGGGTA TGGCAAAAGC TTCTTTAGAA GCTGGTGTTC GTTACTTGGC ATCAAGTTTG GGTGTTGACG GTATCCGTGT AAACGCAATT TCTGCGGGTC CAATTCGTAC TTTAGCTGCT TCTGGTATTA AATCTTTCCG TAAAATGTTA GATGCTAACG AAAAAGTTGC ACCGTTAAAG CGTAACGTAA CCATTGAAGA AGTGGGTAAT GCAGCGTTAT TCCTTTGCTC ACCTTGGGCT TCAGGTATTA CTGGTGAAAT TCTATATGTA GATGCTGGTT TCAATACTGT AGGTATGAGC CAATCTATGA TGGATGATGA ATAA
Details for AcbaC.00170.a.B1.GE38605
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT QGLLAGKRFL IAGVASKLSI AYGIAQALHR EGAELAFTYP NEKLKKRVDE FAEQFGSKLV FPCDVAVDAE IDNAFAELAK HWDGVDGVVH SIGFAPAHTL DGDFTDVTDR DGFKIAHDIS AYSFVAMARA AKPLLQARQG CLLTLTYQGS ERVMPNYNVM GMAKASLEAG VRYLASSLGV DGIRVNAISA GPIRTLAASG IKSFRKMLDA NEKVAPLKRN VTIEEVGNAA LFLCSPWASG ITGEILYVDA GFNTVGMSQX MMDDE
Validated NT Sequence
atcttactca cttattcatc atccatcata gnttggctca tacctacagt attgaaacca gcatctacat atagaatttc accagtaata cctgaagccc aaggtgagca aaggaataac gctgcattac ccacttcttc aatggttacg ttacgcttta acggtgcaac tttttcgtta gcatctaaca ttttacggaa agatttaata ccagaagcag ctaaagtacg aattggaccc gcagaaattg cgtttacacg gataccgtca acacccaaac ttgatgccaa gtaacgaaca ccagcttcta aagaagcttt tgccataccc attacgttgt agttaggcat aacgcgctca gaaccttggt aagtcaaagt taataaacaa ccttggcgag cttgtaacaa aggttttgca gcacgtgcca ttgcaacaaa gctgtatgcg ctaatgtcat gagcaatttt aaaaccgtca cggtcagtca cgtcagtgaa gtcaccatca agcgtgtgtg caggtgcaaa gccgattgag tgcacaacac catctacgcc gtcccaatgt tttgcaagtt ccgcaaatgc attatcaatt tcagcatcaa cggcaacatc acatggaaac acaagcttag aaccgaattg ctcagcaaac tcatctacac gtttttttaa tttttcgttt ggataagtaa atgcaagctc agcaccttca cggtgtaatg cttgtgcaat accataagca attgataatt tactagcaac gcctgcaatc aaaaagcgtt tacctgctaa aagtccttgt gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT QGLLAGKRFL IAGVASKLSI AYGIAQALHR EGAELAFTYP NEKLKKRVDE FAEQFGSKLV FPCDVAVDAE IDNAFAELAK HWDGVDGVVH SIGFAPAHTL DGDFTDVTDR DGFKIAHDIS AYSFVAMARA AKPLLQARQG CLLTLTYQGS ERVMPNYNVM GMAKASLEAG VRYLASSLGV DGIRVNAISA GPIRTLAASG IKSFRKMLDA NEKVAPLKRN VTIEEVGNAA LFLCSPWASG ITGEILYVDA GFNTVGMSQS MMDDE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac aaggactttt agcaggtaaa cgctttttga ttgcaggcgt tgctagtaaa ttatcaattg cttatggtat tgcacaagca ttacaccgtg aaggtgctga gcttgcattt acttatccaa acgaaaaatt aaaaaaacgt gtagatgagt ttgctgagca attcggttct aagcttgtgt ttccatgtga tgttgccgtt gatgctgaaa ttgataatgc atttgcggaa cttgcaaaac attgggacgg cgtagatggt gttgtgcact caatcggctt tgcacctgca cacacgcttg atggtgactt cactgacgtg actgaccgtg acggttttaa aattgctcat gacattagcg catacagctt tgttgcaatg gcacgtgctg caaaaccttt gttacaagct cgccaaggtt gtttattaac tttgacttac caaggttctg agcgcgttat gcctaactac aacgtaatgg gtatggcaaa agcttcttta gaagctggtg ttcgttactt ggcatcaagt ttgggtgttg acggtatccg tgtaaacgca atttctgcgg gtccaattcg tactttagct gcttctggta ttaaatcttt ccgtaaaatg ttagatgcta acgaaaaagt tgcaccgtta aagcgtaacg taaccattga agaagtgggt aatgcagcgt tattcctttg ctcaccttgg gcttcaggta ttactggtga aattctatat gtagatgctg gtttcaatac tgtaggtatg agccaatcta tgatggatga tgaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.00170.a.B1.PW37673
| PURIFICATION DATe: | 3/16/2015 |
| CONCENTRATION: | 25mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QGLLAGKRFL IAGVASKLSI AYGIAQALHR EGAELAFTYP NEKLKKRVDE FAEQFGSKLV FPCDVAVDAE IDNAFAELAK HWDGVDGVVH SIGFAPAHTL DGDFTDVTDR DGFKIAHDIS AYSFVAMARA AKPLLQARQG CLLTLTYQGS ERVMPNYNVM GMAKASLEAG VRYLASSLGV DGIRVNAISA GPIRTLAASG IKSFRKMLDA NEKVAPLKRN VTIEEVGNAA LFLCSPWASG ITGEILYVDA GFNTVGMSQX MMDDE
Validated NT Sequence
atcttactca cttattcatc atccatcata gnttggctca tacctacagt attgaaacca gcatctacat atagaatttc accagtaata cctgaagccc aaggtgagca aaggaataac gctgcattac ccacttcttc aatggttacg ttacgcttta acggtgcaac tttttcgtta gcatctaaca ttttacggaa agatttaata ccagaagcag ctaaagtacg aattggaccc gcagaaattg cgtttacacg gataccgtca acacccaaac ttgatgccaa gtaacgaaca ccagcttcta aagaagcttt tgccataccc attacgttgt agttaggcat aacgcgctca gaaccttggt aagtcaaagt taataaacaa ccttggcgag cttgtaacaa aggttttgca gcacgtgcca ttgcaacaaa gctgtatgcg ctaatgtcat gagcaatttt aaaaccgtca cggtcagtca cgtcagtgaa gtcaccatca agcgtgtgtg caggtgcaaa gccgattgag tgcacaacac catctacgcc gtcccaatgt tttgcaagtt ccgcaaatgc attatcaatt tcagcatcaa cggcaacatc acatggaaac acaagcttag aaccgaattg ctcagcaaac tcatctacac gtttttttaa tttttcgttt ggataagtaa atgcaagctc agcaccttca cggtgtaatg cttgtgcaat accataagca attgataatt tactagcaac gcctgcaatc aaaaagcgtt tacctgctaa aagtccttgt gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT QGLLAGKRFL IAGVASKLSI AYGIAQALHR EGAELAFTYP NEKLKKRVDE FAEQFGSKLV FPCDVAVDAE IDNAFAELAK HWDGVDGVVH SIGFAPAHTL DGDFTDVTDR DGFKIAHDIS AYSFVAMARA AKPLLQARQG CLLTLTYQGS ERVMPNYNVM GMAKASLEAG VRYLASSLGV DGIRVNAISA GPIRTLAASG IKSFRKMLDA NEKVAPLKRN VTIEEVGNAA LFLCSPWASG ITGEILYVDA GFNTVGMSQS MMDDE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC AAGGACTTTT AGCAGGTAAA CGCTTTTTGA TTGCAGGCGT TGCTAGTAAA TTATCAATTG CTTATGGTAT TGCACAAGCA TTACACCGTG AAGGTGCTGA GCTTGCATTT ACTTATCCAA ACGAAAAATT AAAAAAACGT GTAGATGAGT TTGCTGAGCA ATTCGGTTCT AAGCTTGTGT TTCCATGTGA TGTTGCCGTT GATGCTGAAA TTGATAATGC ATTTGCGGAA CTTGCAAAAC ATTGGGACGG CGTAGATGGT GTTGTGCACT CAATCGGCTT TGCACCTGCA CACACGCTTG ATGGTGACTT CACTGACGTG ACTGACCGTG ACGGTTTTAA AATTGCTCAT GACATTAGCG CATACAGCTT TGTTGCAATG GCACGTGCTG CAAAACCTTT GTTACAAGCT CGCCAAGGTT GTTTATTAAC TTTGACTTAC CAAGGTTCTG AGCGCGTTAT GCCTAACTAC AACGTAATGG GTATGGCAAA AGCTTCTTTA GAAGCTGGTG TTCGTTACTT GGCATCAAGT TTGGGTGTTG ACGGTATCCG TGTAAACGCA ATTTCTGCGG GTCCAATTCG TACTTTAGCT GCTTCTGGTA TTAAATCTTT CCGTAAAATG TTAGATGCTA ACGAAAAAGT TGCACCGTTA AAGCGTAACG TAACCATTGA AGAAGTGGGT AATGCAGCGT TATTCCTTTG CTCACCTTGG GCTTCAGGTA TTACTGGTGA AATTCTATAT GTAGATGCTG GTTTCAATAC TGTAGGTATG AGCCAATCTA TGATGGATGA TGAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT