AcbaC.00169.a

replicative DNA helicase

CENTER ID: AcbaC.00169.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
AcbaC.00169.a.B1.GE38604 full length 1 481
AcbaC.00169.a.B3.GE40504 truncation 2 35 467
AcbaC.00169.a.B4.GE40505 truncation 3 19 358
AcbaC.00169.a.B5.GE40506 truncation 4 19 394
AcbaC.00169.a.B6.GE40507 truncation 5 46 481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1500
GenBank: AKA31283.1
PGAT: acinetobacter:ABUW_1545
RefSeq: WP_000075891.1
UniProt: V5VBE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQANATSLT LSSPTENKTS ESGQLKEFRT PPHNLAIEQA VLAALMTVAE SFEQVSDLLK ENDFYATRHK YIYRAIEKLA QENSPYDAVL VNDWLLKQNL LDAIGGEEYL MQLMADSPSS FYNLETYATK IKEFSTLRSM IKVSTEILQN AYDTKGRGVS EILDLAETSI FSLAEQHNNN KKDSGPKAIS SVTADVISKL DELSKLDGNI TGLTTGFLEL DNKTSGMQPG DLIIVAARPS MGKTTFAMNL VESVLQYNKL PALVFSMEMP ADSIAMRLIS AMGKVHQGHL RSGNLDADEW TKVTGTILQL QQMHLYIDDS SALPPTEVRA RARRIAKMHD GKIGCIMVDY LQLMKVPGMG DNRVGEISEI SRSLKALAKE MNCPVIALSQ LNRSLENRPN KRPVMSDLRE SGAIEQDADL IMFIYRDEVY NKESKEAGTA EIIIGKQRNG PIGTVRLAFE GQYTRFSNLS PEYYSQYDDE E
NT Sequence
ATGTCTCAGG CGAATGCCAC TTCTTTAACC CTTTCATCTC CAACAGAAAA TAAGACGAGT GAATCAGGCC AACTTAAAGA GTTTCGCACT CCCCCACACA ATTTAGCCAT TGAGCAAGCT GTACTTGCTG CGCTGATGAC CGTTGCTGAA TCTTTTGAAC AAGTCAGTGA CTTACTCAAA GAAAATGATT TTTATGCCAC TCGTCATAAA TATATTTATC GTGCAATTGA AAAATTAGCA CAGGAAAACT CACCGTATGA TGCGGTTTTG GTCAACGACT GGCTTTTAAA ACAAAATTTA CTCGATGCGA TTGGCGGTGA AGAATATTTG ATGCAGCTTA TGGCTGACTC ACCTTCGAGT TTCTATAATC TTGAAACTTA TGCGACTAAA ATTAAAGAAT TTTCTACACT ACGCAGTATG ATTAAGGTAA GTACTGAAAT TTTACAAAAT GCTTACGACA CAAAAGGTCG AGGTGTAAGT GAAATTTTAG ATTTAGCCGA AACGAGTATT TTCTCTTTAG CAGAACAACA TAACAACAAT AAAAAAGATT CTGGACCTAA AGCAATTAGT TCTGTAACTG CCGATGTAAT TAGTAAACTT GATGAACTTT CAAAATTAGA CGGAAATATT ACTGGTTTAA CGACTGGCTT CTTAGAGCTC GATAATAAAA CCTCTGGTAT GCAACCAGGT GATTTGATTA TTGTCGCAGC ACGTCCATCT ATGGGTAAAA CTACTTTCGC CATGAACTTG GTTGAAAGTG TTTTGCAATA CAATAAACTC CCAGCTCTCG TCTTCTCTAT GGAGATGCCA GCAGACTCTA TTGCGATGCG TCTCATTTCA GCTATGGGTA AAGTGCATCA AGGGCATTTA CGTTCAGGTA ATTTGGATGC TGATGAATGG ACTAAAGTAA CGGGTACCAT TCTGCAACTT CAACAAATGC ATTTGTATAT TGATGATTCC TCTGCCCTAC CACCTACTGA AGTTCGTGCG CGAGCTAGAC GTATCGCAAA AATGCATGAC GGTAAAATCG GCTGTATCAT GGTCGACTAT TTACAGTTGA TGAAAGTTCC GGGCATGGGT GACAACCGTG TTGGTGAGAT CTCGGAAATT TCACGTAGCT TAAAAGCTTT AGCTAAAGAA ATGAACTGTC CTGTGATTGC ACTATCTCAG CTTAACCGAA GTCTGGAAAA CCGACCGAAT AAACGTCCAG TAATGTCTGA CTTACGTGAA TCTGGTGCGA TTGAACAGGA TGCCGACTTA ATCATGTTTA TTTACCGTGA TGAAGTCTAT AACAAAGAAT CGAAAGAAGC AGGCACCGCA GAAATTATCA TCGGTAAACA GCGTAATGGT CCAATCGGTA CTGTTCGCCT TGCCTTTGAA GGCCAATATA CTCGTTTTAG TAATCTCTCT CCTGAGTATT ACAGCCAATA TGATGATGAA GAATAA
Details for AcbaC.00169.a.B1.GE38604
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS QANATSLTLS SPTENKTSES GQLKEFRTPP HNLAIEQAVL AALMTVAESF EQVSDLLKEN DFYATRHKYI YRAIEKLAQE NSPYDAVLVN DWLLKQNLLD AIGGEEYLMQ LMADSPSSFY NLETYATKIK EFSTLRSMIK VSTEILQNAY DTKGRGVSEI LDLAETSIFS LAEQHNNNKK DSGPKAISSV TADVISKLDE LSKLDGNITG LTTGFLELDN KTSGMQPGDL IIVAARPSMG KTTFAMNLVE SVLQYNKLPA LVFSMEMPAD SIAMRLISAM GKVHQGHLRS GNLDADEWTK VTGTILQLQQ MHLYIDDSSA LPPTEVRARA RRIAKMHDGK IGCIMVDYLQ LMKVPGMGDN RVGEISEISR SLKALAKEMN CPVIALSQLN RSLENRPNKR PVMSDLRESG AIEQDADLIM FIYRDEVYNK ESKEAGTAEI IIGKQRNGPI GTVRLAFEGQ YTRFSNLSPE Y
Validated NT Sequence
aatactcagg agagagatta ctaaaacgag tatattggcc ttcaaaggca aggcgaacag taccgattgg accattacgc tgtttaccga tgataatttc tgcggtgcct gcttctttcg attctttgtt atagacttca tcacggtaaa taaacatgat taagtcggca tcctgttcaa tcgcaccaga ttcacgtaag tcagacatta ctggacgttt attcggtcgg ttttccagac ttcggttaag ctgagatagt gcaatcacag gacagttcat ttctttagct aaagctttta agctacgtga aatttccgag atctcaccaa cacggttgtc acccatgccc ggaactttca tcaactgtaa atagtcgacc atgatacagc cgattttacc gtcatgcatt tttgcgatac gtctagctcg cgcacgaact tcagtaggtg gtagggcaga ggaatcatca atatacaaat gcatttgttg aagttgcaga atggtacccg ttactttagt ccattcatca gcatccaaat tacctgaacg taaatgccct tgatgcactt tacccatagc tgaaatgaga cgcatcgcaa tagagtctgc tggcatctcc atagagaaga cgagagctgg gagtttattg tattgcaaaa cactttcaac caagttcatg gcgaaagtag ttttacccat agatggacgt gctgcgacaa taatcaaatc acctggttgc ataccagagg ttttattatc gagctctaag aagccagtcg ttaaaccagt aatatttccg tctaattttg aaagttcatc aagtttacta attacatcgg cagttacaga actaattgct ttaggtccag aatctttttt attgttgtta tgttgttctg ctaaagagaa aatactcgtt tcggctaaat ctaaaatttc acttacacct cgaccttttg tgtcgtaagc attttgtaaa atttcagtac ttaccttaat catactgcgt agtgtagaaa attctttaat tttagtcgca taagtttcaa gattatagaa actcgaaggt gagtcagcca taagctgcat caaatattct tcaccgccaa tcgcatcgag taaattttgt tttaaaagcc agtcgttgac caaaaccgca tcatacggtg agttttcctg tgctaatttt tcaattgcac gataaatata tttatgacga gtggcataaa aatcattttc tttgagtaag tcactgactt gttcaaaaga ttcagcaacg gtcatcagcg cagcaagtac agcttgctca atggctaaat tgtgtggggg agtgcgaaac tctttaagtt ggcctgattc actcgtctta ttttctgttg gagatgaaag ggttaaagaa gtggcattcg cctgagacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS QANATSLTLS SPTENKTSES GQLKEFRTPP HNLAIEQAVL AALMTVAESF EQVSDLLKEN DFYATRHKYI YRAIEKLAQE NSPYDAVLVN DWLLKQNLLD AIGGEEYLMQ LMADSPSSFY NLETYATKIK EFSTLRSMIK VSTEILQNAY DTKGRGVSEI LDLAETSIFS LAEQHNNNKK DSGPKAISSV TADVISKLDE LSKLDGNITG LTTGFLELDN KTSGMQPGDL IIVAARPSMG KTTFAMNLVE SVLQYNKLPA LVFSMEMPAD SIAMRLISAM GKVHQGHLRS GNLDADEWTK VTGTILQLQQ MHLYIDDSSA LPPTEVRARA RRIAKMHDGK IGCIMVDYLQ LMKVPGMGDN RVGEISEISR SLKALAKEMN CPVIALSQLN RSLENRPNKR PVMSDLRESG AIEQDADLIM FIYRDEVYNK ESKEAGTAEI IIGKQRNGPI GTVRLAFEGQ YTRFSNLSPE YYSQYDDEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc aggcgaatgc cacttcttta accctttcat ctccaacaga aaataagacg agtgaatcag gccaacttaa agagtttcgc actcccccac acaatttagc cattgagcaa gctgtacttg ctgcgctgat gaccgttgct gaatcttttg aacaagtcag tgacttactc aaagaaaatg atttttatgc cactcgtcat aaatatattt atcgtgcaat tgaaaaatta gcacaggaaa actcaccgta tgatgcggtt ttggtcaacg actggctttt aaaacaaaat ttactcgatg cgattggcgg tgaagaatat ttgatgcagc ttatggctga ctcaccttcg agtttctata atcttgaaac ttatgcgact aaaattaaag aattttctac actacgcagt atgattaagg taagtactga aattttacaa aatgcttacg acacaaaagg tcgaggtgta agtgaaattt tagatttagc cgaaacgagt attttctctt tagcagaaca acataacaac aataaaaaag attctggacc taaagcaatt agttctgtaa ctgccgatgt aattagtaaa cttgatgaac tttcaaaatt agacggaaat attactggtt taacgactgg cttcttagag ctcgataata aaacctctgg tatgcaacca ggtgatttga ttattgtcgc agcacgtcca tctatgggta aaactacttt cgccatgaac ttggttgaaa gtgttttgca atacaataaa ctcccagctc tcgtcttctc tatggagatg ccagcagact ctattgcgat gcgtctcatt tcagctatgg gtaaagtgca tcaagggcat ttacgttcag gtaatttgga tgctgatgaa tggactaaag taacgggtac cattctgcaa cttcaacaaa tgcatttgta tattgatgat tcctctgccc taccacctac tgaagttcgt gcgcgagcta gacgtatcgc aaaaatgcat gacggtaaaa tcggctgtat catggtcgac tatttacagt tgatgaaagt tccgggcatg ggtgacaacc gtgttggtga gatctcggaa atttcacgta gcttaaaagc tttagctaaa gaaatgaact gtcctgtgat tgcactatct cagcttaacc gaagtctgga aaaccgaccg aataaacgtc cagtaatgtc tgacttacgt gaatctggtg cgattgaaca ggatgccgac ttaatcatgt ttatttaccg tgatgaagtc tataacaaag aatcgaaaga agcaggcacc gcagaaatta tcatcggtaa acagcgtaat ggtccaatcg gtactgttcg ccttgccttt gaaggccaat atactcgttt tagtaatctc tctcctgagt attacagcca atatgatgat gaagaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for AcbaC.00169.a.B3.GE40504
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLA IEQAVLAALM TVAESFEQVS DLLKENDFYA TRHKYIYRAI EKLAQENSPY DAVLVNDWLL KQNLLDAIGG EEYLMQLMAD SPSSFYNLET YATKIKEFST LRSMIKVSTE ILQNAYDTKG RGVSEILDLA ETSIFSLAEQ HNNNKKDSGP KAISSVTADV ISKLDELSKL DGNITGLTTG FLELDNKTSG MQPGDLIIVA ARPSMGKTTF AMNLVESVLQ YNKLPALVFS MEMPADSIAM RLISAMGKVH QGHLRSGNLD ADEWTKVTGT ILQLQQMHLY IDDSSALPPT EVRARARRIA KMHDGKIGCI MVDYLQLMKV PGMGDNRVGE ISEISRSLKA LAKEMNCPVI ALSQLNRSLE NRPNKRPVMS DLRESGAIEQ DADLIMFIYR DEVYNKESKE AGTAEIIIGK QRNGPIGTVR LAFEGQYTRF S
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttagcca ttgagcaagc tgtacttgct gcgctgatga ccgttgctga atcttttgaa caagtcagtg acttactcaa agaaaatgat ttttatgcca ctcgtcataa atatatttat cgtgcaattg aaaaattagc acaggaaaac tcaccgtatg atgcggtttt ggtcaacgac tggcttttaa aacaaaattt actcgatgcg attggcggtg aagaatattt gatgcagctt atggctgact caccttcgag tttctataat cttgaaactt atgcgactaa aattaaagaa ttttctacac tacgcagtat gattaaggta agtactgaaa ttttacaaaa tgcttacgac acaaaaggtc gaggtgtaag tgaaatttta gatttagccg aaacgagtat tttctcttta gcagaacaac ataacaacaa taaaaaagat tctggaccta aagcaattag ttctgtaact gccgatgtaa ttagtaaact tgatgaactt tcaaaattag acggaaatat tactggttta acgactggct tcttagagct cgataataaa acctctggta tgcaaccagg tgatttgatt attgtcgcag cacgtccatc tatgggtaaa actactttcg ccatgaactt ggttgaaagt gttttgcaat acaataaact cccagctctc gtcttctcta tggagatgcc agcagactct attgcgatgc gtctcatttc agctatgggt aaagtgcatc aagggcattt acgttcaggt aatttggatg ctgatgaatg gactaaagta acgggtacca ttctgcaact tcaacaaatg catttgtata ttgatgattc ctctgcccta ccacctactg aagttcgtgc gcgagctaga cgtatcgcaa aaatgcatga cggtaaaatc ggctgtatca tggtcgacta tttacagttg atgaaagttc cgggcatggg tgacaaccgt gttggtgaga tctcggaaat ttcacgtagc ttaaaagctt tagctaaaga aatgaactgt cctgtgattg cactatctca gcttaaccga agtctggaaa accgaccgaa taaacgtcca gtaatgtctg acttacgtga atctggtgcg attgaacagg atgccgactt aatcatgttt atttaccgtg atgaagtcta taacaaagaa tcgaaagaag caggcaccgc agaaattatc atcggtaaac agcgtaatgg tccaatcggt actgttcgcc ttgcctttga aggccaatat actcgtttta gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for AcbaC.00169.a.B4.GE40505
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHTS ESGQLKEFRT PPHNLAIEQA VLAALMTVAE SFEQVSDLLK ENDFYATRHK YIYRAIEKLA QENSPYDAVL VNDWLLKQNL LDAIGGEEYL MQLMADSPSS FYNLETYATK IKEFSTLRSM IKVSTEILQN AYDTKGRGVS EILDLAETSI FSLAEQHNNN KKDSGPKAIS SVTADVISKL DELSKLDGNI TGLTTGFLEL DNKTSGMQPG DLIIVAARPS MGKTTFAMNL VESVLQYNKL PALVFSMEMP ADSIAMRLIS AMGKVHQGHL RSGNLDADEW TKVTGTILQL QQMHLYIDDS SALPPTEVRA RARRIAKMHD GKIGCIMVDY LQLMKVPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgagtg aatcaggcca acttaaagag tttcgcactc ccccacacaa tttagccatt gagcaagctg tacttgctgc gctgatgacc gttgctgaat cttttgaaca agtcagtgac ttactcaaag aaaatgattt ttatgccact cgtcataaat atatttatcg tgcaattgaa aaattagcac aggaaaactc accgtatgat gcggttttgg tcaacgactg gcttttaaaa caaaatttac tcgatgcgat tggcggtgaa gaatatttga tgcagcttat ggctgactca ccttcgagtt tctataatct tgaaacttat gcgactaaaa ttaaagaatt ttctacacta cgcagtatga ttaaggtaag tactgaaatt ttacaaaatg cttacgacac aaaaggtcga ggtgtaagtg aaattttaga tttagccgaa acgagtattt tctctttagc agaacaacat aacaacaata aaaaagattc tggacctaaa gcaattagtt ctgtaactgc cgatgtaatt agtaaacttg atgaactttc aaaattagac ggaaatatta ctggtttaac gactggcttc ttagagctcg ataataaaac ctctggtatg caaccaggtg atttgattat tgtcgcagca cgtccatcta tgggtaaaac tactttcgcc atgaacttgg ttgaaagtgt tttgcaatac aataaactcc cagctctcgt cttctctatg gagatgccag cagactctat tgcgatgcgt ctcatttcag ctatgggtaa agtgcatcaa gggcatttac gttcaggtaa tttggatgct gatgaatgga ctaaagtaac gggtaccatt ctgcaacttc aacaaatgca tttgtatatt gatgattcct ctgccctacc acctactgaa gttcgtgcgc gagctagacg tatcgcaaaa atgcatgacg gtaaaatcgg ctgtatcatg gtcgactatt tacagttgat gaaagttccg ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.00169.a.B5.GE40506
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT SESGQLKEFR TPPHNLAIEQ AVLAALMTVA ESFEQVSDLL KENDFYATRH KYIYRAIEKL AQENSPYDAV LVNDWLLKQN LLDAIGGEEY LMQLMADSPS SFYNLETYAT KIKEFSTLRS MIKVSTEILQ NAYDTKGRGV SEILDLAETS IFSLAEQHNN NKKDSGPKAI SSVTADVISK LDELSKLDGN ITGLTTGFLE LDNKTSGMQP GDLIIVAARP SMGKTTFAMN LVESVLQYNK LPALVFSMEM PADSIAMRLI SAMGKVHQGH LRSGNLDADE WTKVTGTILQ LQQMHLYIDD SSALPPTEVR ARARRIAKMH DGKIGCIMXX YLQLMKVPGM GDNRVGEISE IXXXXXALAK EMN
Validated NT Sequence
atggctcacc accaccacca ccatatgacg agtgaatcag gccaacttaa agagtttcgc actcccccac acaatttagc cattgagcaa gctgtacttg ctgcgctgat gaccgttgct gaatcttttg aacaagtcag tgacttactc aaagaaaatg atttttatgc cactcgtcat aaatatattt atcgtgcaat tgaaaaatta gcacaggaaa actcaccgta tgatgcggtt ttggtcaacg actggctttt aaaacaaaat ttactcgatg cgattggcgg tgaagaatat ttgatgcagc ttatggctga ctcaccttcg agtttctata atcttgaaac ttatgcgact aaaattaaag aattttctac actacgcagt atgattaagg taagtactga aattttacaa aatgcttacg acacaaaagg tcgaggtgta agtgaaattt tagatttagc cgaaacgagt attttctctt tagcagaaca acataacaac aataaaaaag attctggacc taaagcaatt agttctgtaa ctgccgatgt aattagtaaa cttgatgaac tttcaaaatt agacggaaat attactggtt taacgactgg cttcttagag ctcgataata aaacctctgg tatgcaacca ggtgatttga ttattgtcgc agcacgtcca tctatgggta aaactacttt cgccatgaac ttggttgaaa gtgttttgca atacaataaa ctcccagctc tcgtcttctc tatggagatg ccagcagact ctattgcgat gcgtctcatt tcagctatgg gtaaagtgca tcaagggcat ttacgttcag gtaatttgga tgctgatgaa tggactaaag taacgggtac cattctgcaa cttcaacaaa tgcatttgta tattgatgat tcctctgccc taccacctac tgaagttcgt gcgcgagcta gacgtatcgc aaaaatgcat gacggtaaaa tcggctgtat catgnnnnac tatttacagt tgatgaaagt tccgggcatg ggtgacaacc gtgttggtga gatctcggaa atttnnnnnn nnnnnnnagc tttagcnaaa gaaatgaact gt
Expected Protein Sequence
MAHHHHHHTS ESGQLKEFRT PPHNLAIEQA VLAALMTVAE SFEQVSDLLK ENDFYATRHK YIYRAIEKLA QENSPYDAVL VNDWLLKQNL LDAIGGEEYL MQLMADSPSS FYNLETYATK IKEFSTLRSM IKVSTEILQN AYDTKGRGVS EILDLAETSI FSLAEQHNNN KKDSGPKAIS SVTADVISKL DELSKLDGNI TGLTTGFLEL DNKTSGMQPG DLIIVAARPS MGKTTFAMNL VESVLQYNKL PALVFSMEMP ADSIAMRLIS AMGKVHQGHL RSGNLDADEW TKVTGTILQL QQMHLYIDDS SALPPTEVRA RARRIAKMHD GKIGCIMVDY LQLMKVPGMG DNRVGEISEI SRSLKALAKE MNCPVIALSQ LNRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacgagtg aatcaggcca acttaaagag tttcgcactc ccccacacaa tttagccatt gagcaagctg tacttgctgc gctgatgacc gttgctgaat cttttgaaca agtcagtgac ttactcaaag aaaatgattt ttatgccact cgtcataaat atatttatcg tgcaattgaa aaattagcac aggaaaactc accgtatgat gcggttttgg tcaacgactg gcttttaaaa caaaatttac tcgatgcgat tggcggtgaa gaatatttga tgcagcttat ggctgactca ccttcgagtt tctataatct tgaaacttat gcgactaaaa ttaaagaatt ttctacacta cgcagtatga ttaaggtaag tactgaaatt ttacaaaatg cttacgacac aaaaggtcga ggtgtaagtg aaattttaga tttagccgaa acgagtattt tctctttagc agaacaacat aacaacaata aaaaagattc tggacctaaa gcaattagtt ctgtaactgc cgatgtaatt agtaaacttg atgaactttc aaaattagac ggaaatatta ctggtttaac gactggcttc ttagagctcg ataataaaac ctctggtatg caaccaggtg atttgattat tgtcgcagca cgtccatcta tgggtaaaac tactttcgcc atgaacttgg ttgaaagtgt tttgcaatac aataaactcc cagctctcgt cttctctatg gagatgccag cagactctat tgcgatgcgt ctcatttcag ctatgggtaa agtgcatcaa gggcatttac gttcaggtaa tttggatgct gatgaatgga ctaaagtaac gggtaccatt ctgcaacttc aacaaatgca tttgtatatt gatgattcct ctgccctacc acctactgaa gttcgtgcgc gagctagacg tatcgcaaaa atgcatgacg gtaaaatcgg ctgtatcatg gtcgactatt tacagttgat gaaagttccg ggcatgggtg acaaccgtgt tggtgagatc tcggaaattt cacgtagctt aaaagcttta gctaaagaaa tgaactgtcc tgtgattgca ctatctcagc ttaaccgaag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for AcbaC.00169.a.B6.GE40507
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT VAESFEQVSD LLKENDFYAT RHKYIYRAIE KXAQENSPYD AVLVNDWLLK QNLLDAIGXX EYLMQLMADS PSSFYNLETY ATKIKEFSTL RXMIKVSTEI LQNAYXTKGR GVSEILDLAE TSIFSLAEQH NNNKKDSGPK AISSVTADVI SKLDELSKLD GNITGLTTGF LELDNKTSGM QPGDLIIVAA RPSMGKTTFA MNLVESVLQY NKLPALVFSM EMPADSIAMR LISAMGKVHQ GHLRSGNLDA DEWTKVTGTI LQLQQMHLYI DDSSALPPTE VRARARRIAK MHDGKIGCIM VDYLQLMKVP GMGDNRVGEI SEISRSLKAL AKEMNCPVIA LSQLNRSLEN RPNKRPVMSD LREXGAIEQD AXLIMFIYRD EVYNKESKEA GTAEIIIGKQ RNGPIXXVRL AFEXXYTXXS NLSX
Validated NT Sequence
atggctcacc accaccacca ccatatgacc gttgctgaat cttttgaaca agtcagtgac ttactcaaag aaaatgattt ttatgccact cgtcataaat atatttatcg tgcaattgaa aaattngcac aggaaaactc accgtatgat gcggttttgg tcaacgactg gcttttaaaa caaaatttac tcgatgcgat tggcgnnnan gaatatttga tgcagcttat ggctgactca ccttcgagtt tctataatct tgaaacttat gcgactaaaa ttaaagaatt ttctacacta cgcnnnatga ttaaggtaag tactgaaatt ttacaaaatg cttacgncac aaaaggtcga ggtgtaagtg aaattttaga tttagccgaa acgagtattt tctctttagc agaacaacat aacaacaata aaaaagattc tggacctaaa gcaattagtt ctgtaactgc cgatgtaatt agtaaacttg atgaactttc aaaattagac ggaaatatta ctggtttaac gactggcttc ttagagctcg ataataaaac ctctggtatg caaccaggtg atttgattat tgtcgcagca cgtccatcta tgggtaaaac tactttcgcc atgaacttgg ttgaaagtgt tttgcaatac aataaactcc cagctctcgt cttctctatg gagatgccag cagactctat tgcgatgcgt ctcatttcag ctatgggtaa agtgcatcaa gggcatttac gttcaggtaa tttggatgct gatgaatgga ctaaagtaac gggtaccatt ctgcaacttc aacaaatgca tttgtatatt gatgattcct ctgccctacc acctactgaa gttcgtgcgc gagctagacg tatcgcaaaa atgcatgacg gtaaaatcgg ctgtatcatg gtcgactatt tacagttgat gaaagttccg ggcatgggtg acaaccgtgt tggtgagatc tcggaaattt cacgtagctt aaaagcttta gctaaagaaa tgaactgtcc tgtgattgca ctatctcagc ttaaccgaag tctggaaaac cgaccgaata aacgtccagt aatgtctgac ttacgtgaan nnggtgcgat tgaacaggat gccgncttaa tcatgtttat ttaccgtgat gaagtctata acaaagaatc gaaagaagca ggcaccgcag aaattatcat cggtaaacag cgtaatggtc caatcnnnnn tgttcgcctg gcctttgaan nnnantatac nnnnnnnagt aatctctctn nnna
Expected Protein Sequence
MAHHHHHHMT VAESFEQVSD LLKENDFYAT RHKYIYRAIE KLAQENSPYD AVLVNDWLLK QNLLDAIGGE EYLMQLMADS PSSFYNLETY ATKIKEFSTL RSMIKVSTEI LQNAYDTKGR GVSEILDLAE TSIFSLAEQH NNNKKDSGPK AISSVTADVI SKLDELSKLD GNITGLTTGF LELDNKTSGM QPGDLIIVAA RPSMGKTTFA MNLVESVLQY NKLPALVFSM EMPADSIAMR LISAMGKVHQ GHLRSGNLDA DEWTKVTGTI LQLQQMHLYI DDSSALPPTE VRARARRIAK MHDGKIGCIM VDYLQLMKVP GMGDNRVGEI SEISRSLKAL AKEMNCPVIA LSQLNRSLEN RPNKRPVMSD LRESGAIEQD ADLIMFIYRD EVYNKESKEA GTAEIIIGKQ RNGPIGTVRL AFEGQYTRFS NLSPEYYSQY DDEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ttgctgaatc ttttgaacaa gtcagtgact tactcaaaga aaatgatttt tatgccactc gtcataaata tatttatcgt gcaattgaaa aattagcaca ggaaaactca ccgtatgatg cggttttggt caacgactgg cttttaaaac aaaatttact cgatgcgatt ggcggtgaag aatatttgat gcagcttatg gctgactcac cttcgagttt ctataatctt gaaacttatg cgactaaaat taaagaattt tctacactac gcagtatgat taaggtaagt actgaaattt tacaaaatgc ttacgacaca aaaggtcgag gtgtaagtga aattttagat ttagccgaaa cgagtatttt ctctttagca gaacaacata acaacaataa aaaagattct ggacctaaag caattagttc tgtaactgcc gatgtaatta gtaaacttga tgaactttca aaattagacg gaaatattac tggtttaacg actggcttct tagagctcga taataaaacc tctggtatgc aaccaggtga tttgattatt gtcgcagcac gtccatctat gggtaaaact actttcgcca tgaacttggt tgaaagtgtt ttgcaataca ataaactccc agctctcgtc ttctctatgg agatgccagc agactctatt gcgatgcgtc tcatttcagc tatgggtaaa gtgcatcaag ggcatttacg ttcaggtaat ttggatgctg atgaatggac taaagtaacg ggtaccattc tgcaacttca acaaatgcat ttgtatattg atgattcctc tgccctacca cctactgaag ttcgtgcgcg agctagacgt atcgcaaaaa tgcatgacgg taaaatcggc tgtatcatgg tcgactattt acagttgatg aaagttccgg gcatgggtga caaccgtgtt ggtgagatct cggaaatttc acgtagctta aaagctttag ctaaagaaat gaactgtcct gtgattgcac tatctcagct taaccgaagt ctggaaaacc gaccgaataa acgtccagta atgtctgact tacgtgaatc tggtgcgatt gaacaggatg ccgacttaat catgtttatt taccgtgatg aagtctataa caaagaatcg aaagaagcag gcaccgcaga aattatcatc ggtaaacagc gtaatggtcc aatcggtact gttcgccttg cctttgaagg ccaatatact cgttttagta atctctctcc tgagtattac agccaatatg atgatgaaga atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat