SSGCID: Target Page

AcbaC.00164.a

arginyl-tRNA synthetase

CENTER ID:	AcbaC.00164.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00164.a.B1.GE38603	full length		1	596

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00164.a.B1.PW37644	full length		1	596

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3668
GenBank:	AKA33422.1
PGAT:	acinetobacter:ABUW_3747
RefSeq:	WP_001090284.1
UniProt:	V5VI16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTAIQAALD HAVQTLQQEG VLPSDWNNSS NLTRTKDRSH GDFASNIAMI GSKAAGMKPR DLAEKILAAL PEVADISKAE IAGPGFINFF LNADQRFAIL DQIQAQKESF GRSQSNAAKK IQVEFVSANP TSSLHVGHGR GAAYGMTVAN LLEATGAKVD REYYVNDAGR QMDILATSTY LRYLELLGQN LVFPKNAYQG DYVKEIAQGI IDKDGDAYVR EVANVYKDVP EDVQYAEELD SEGNKVVLSG DKEKHIDGLI ANSQQLLGEG YRVFHQAALH AILDDIKDDL ADFGVTFNQW FSEASLSAKI DEALETLDQR GFLYEKDGNI WFKSTEFGDE KDRVVKRRNG QTTYFASDIA YHLNKLQRGY TDLVDIWGSD HHGYISRVKA AIDAMGYDSK KLTVLLVQFV SLWRGGEMVQ MSSRSGQFVT LRDLRKEVGN DAARFYYVMR KSEQHIDFDL DLAVSQSKDN AVYYIQYAHA RICRMLEKAA STGLQFEVSA ARSHAARLSL DAETEILAKL AAYPDVVLRA ANAYEPHQVG NYLKELAALF HGWYNEHKVL SDDAELTQAR LLLSINVQQV LRNGLELLGV SAPEAM

NT Sequence

ATGAATACGG CAATACAAGC AGCTCTCGAC CATGCAGTGC AAACCCTTCA GCAAGAAGGT GTGCTTCCTT CGGATTGGAA TAATTCAAGC AATTTAACGC GCACCAAAGA CCGAAGCCAT GGCGACTTTG CTTCTAATAT TGCCATGATC GGCTCTAAAG CCGCTGGTAT GAAACCACGC GATTTAGCTG AGAAAATTCT CGCAGCGCTA CCAGAAGTTG CAGACATCAG CAAAGCTGAA ATCGCAGGCC CTGGTTTTAT CAATTTCTTT TTAAATGCAG ATCAACGTTT TGCAATTCTT GACCAGATTC AGGCTCAAAA AGAAAGCTTT GGTCGTAGCC AATCAAATGC AGCTAAAAAG ATTCAAGTTG AATTCGTTTC TGCAAACCCG ACGTCTAGCC TACACGTAGG TCATGGCCGT GGTGCTGCTT ATGGTATGAC TGTTGCAAAC TTGCTTGAAG CAACAGGTGC TAAAGTTGAT CGCGAATATT ATGTAAATGA TGCTGGCCGT CAAATGGATA TTTTGGCGAC TTCTACTTAT TTACGTTATT TGGAATTACT TGGACAAAAC CTTGTATTCC CTAAAAATGC ATATCAAGGC GATTACGTTA AAGAAATCGC ACAAGGCATC ATTGATAAAG ATGGCGATGC TTACGTTCGT GAAGTTGCGA ATGTTTATAA AGATGTACCA GAAGACGTGC AATATGCTGA AGAGCTAGAT AGCGAAGGCA ACAAAGTCGT ATTATCTGGC GACAAAGAAA AACACATTGA CGGTCTTATT GCCAACTCAC AACAATTATT AGGTGAAGGC TACCGCGTAT TCCATCAAGC CGCACTCCAT GCCATTTTAG ATGATATTAA AGATGACCTT GCTGACTTCG GCGTAACCTT TAATCAATGG TTTAGTGAAG CATCTCTTAG CGCAAAAATT GACGAAGCGT TAGAAACACT TGATCAACGC GGCTTCTTAT ATGAAAAAGA TGGCAACATC TGGTTTAAAT CGACTGAATT TGGTGATGAA AAAGACCGTG TTGTGAAGCG CCGTAATGGT CAAACAACTT ACTTTGCATC TGACATTGCA TATCACTTAA ATAAACTACA ACGTGGTTAT ACTGACCTTG TTGATATTTG GGGTTCTGAC CACCACGGTT ATATCTCTCG CGTAAAAGCT GCGATTGATG CAATGGGTTA TGACTCGAAA AAATTAACTG TTCTTTTAGT TCAGTTCGTA AGCTTATGGC GCGGTGGTGA GATGGTTCAA ATGTCATCTC GTTCAGGTCA GTTCGTGACT TTACGTGACT TGCGTAAAGA AGTCGGCAAT GATGCAGCTC GTTTCTACTA CGTGATGCGT AAAAGTGAAC AACACATTGA CTTTGACCTT GACCTTGCTG TTTCACAAAG TAAAGACAAT GCGGTGTATT ATATTCAATA CGCTCATGCA CGTATTTGCC GTATGCTAGA AAAAGCAGCA AGCACTGGCT TACAATTTGA AGTAAGTGCT GCTCGTTCAC ATGCTGCACG TTTATCACTT GATGCAGAAA CTGAAATTCT TGCTAAACTG GCTGCATATC CAGACGTTGT GTTACGTGCT GCTAATGCAT ATGAACCACA CCAAGTAGGT AACTACTTAA AAGAATTAGC TGCGCTATTC CACGGTTGGT ACAATGAACA CAAAGTTTTA AGTGATGATG CTGAACTTAC ACAAGCGCGC TTATTACTTT CAATCAATGT ACAGCAAGTA TTGCGCAATG GCTTAGAATT ACTCGGTGTT TCTGCACCTG AAGCCATGTA A

Details for AcbaC.00164.a.B1.GE38603

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN TAIQAALDHA VQTLQQEGVL PSDWNNSSNL TRTKDRSHGD FASNIAMIGS KAAGMKPRDL AEKILAALPE VADISKAEIA GPGFINFFLN ADQRFAILDQ IQAQKESFGR SQSNAAKKIQ VEFVSANPTS SLHVGHGRGA AYGMTVANLL EATGAKVDRE YYVNDAGRQM DILATSTYLR YLELLGQNLV FPKNAYQGDY VKEIAQGIID KDGDAYVREV ANVYKDVPED VQYAEELDSE GNKVVLSGDK EKHIDGLIAN SQQLLGEGYR VFHQAALHAI LDDIKDDLAD FGVTFNQWFS EASLSAKIDE ALETLDQRGF LYEKDGNIWF KSTEFGDEKD RVVKRRNGQT TYFASDIAYH LNKLQRGYTD LVDIWGSDHH GYISRVKAAI DAMGYDSKKL TVLLVQFVSL WRGGEMVQMS SRSGQFVTLR DLRKEVGNDA ARFYYVMRKS EQHIDFDLDL AVSQSKDNAV YYIQYAHARI CRMLEKAAST GLQFEVSAAR SHAARLSLDA ETEILAKLAA YPDVVLRAAN AYEPHQVGNY LKELAALFHG WYNEHKVLSD DAELTQARLL LSINVQQVLR NGLELLGVSA PEAM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata cggcaataca agcagctctc gaccatgcag tgcaaaccct tcagcaagaa ggtgtgcttc cttcggattg gaataattca agcaatttaa cgcgcaccaa agaccgaagc catggcgact ttgcttctaa tattgccatg atcggctcta aagccgctgg tatgaaacca cgcgatttag ctgagaaaat tctcgcagcg ctaccagaag ttgcagacat cagcaaagct gaaatcgcag gccctggttt tatcaatttc tttttaaatg cagatcaacg ttttgcaatt cttgaccaga ttcaggctca aaaagaaagc tttggtcgta gccaatcaaa tgcagctaaa aagattcaag ttgaattcgt ttctgcaaac ccgacgtcta gcctacacgt aggtcatggc cgtggtgctg cttatggtat gactgttgca aacttgcttg aagcaacagg tgctaaagtt gatcgcgaat attatgtaaa tgatgctggc cgtcaaatgg atattttggc gacttctact tatttacgtt atttggaatt acttggacaa aaccttgtat tccctaaaaa tgcatatcaa ggcgattacg ttaaagaaat cgcacaaggc atcattgata aagatggcga tgcttacgtt cgtgaagttg cgaatgttta taaagatgta ccagaagacg tgcaatatgc tgaagagcta gatagcgaag gcaacaaagt cgtattatct ggcgacaaag aaaaacacat tgacggtctt attgccaact cacaacaatt attaggtgaa ggctaccgcg tattccatca agccgcactc catgccattt tagatgatat taaagatgac cttgctgact tcggcgtaac ctttaatcaa tggtttagtg aagcatctct tagcgcaaaa attgacgaag cgttagaaac acttgatcaa cgcggcttct tatatgaaaa agatggcaac atctggttta aatcgactga atttggtgat gaaaaagacc gtgttgtgaa gcgccgtaat ggtcaaacaa cttactttgc atctgacatt gcatatcact taaataaact acaacgtggt tatactgacc ttgttgatat ttggggttct gaccaccacg gttatatctc tcgcgtaaaa gctgcgattg atgcaatggg ttatgactcg aaaaaattaa ctgttctttt agttcagttc gtaagcttat ggcgcggtgg tgagatggtt caaatgtcat ctcgttcagg tcagttcgtg actttacgtg acttgcgtaa agaagtcggc aatgatgcag ctcgtttcta ctacgtgatg cgtaaaagtg aacaacacat tgactttgac cttgaccttg ctgtttcaca aagtaaagac aatgcggtgt attatattca atacgctcat gcacgtattt gccgtatgct agaaaaagca gcaagcactg gcttacaatt tgaagtaagt gctgctcgtt cacatgctgc acgtttatca cttgatgcag aaactgaaat tcttgctaaa ctggctgcat atccagacgt tgtgttacgt gctgctaatg catatgaacc acaccaagta ggtaactact taaaagaatt agctgcgcta ttccacggtt ggtacaatga acacaaagtt ttaagtgatg atgctgaact tacacaagcg cgcttattac tttcaatcaa tgtacagcaa gtattgcgca atggcttaga attactcggt gtttctgcac ctgaagccat gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for AcbaC.00164.a.B1.PW37644

PURIFICATION DATe:	2/2/2015
CONCENTRATION:	29mg/ml
OBSERVED MW:	66kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA CGGCAATACA AGCAGCTCTC GACCATGCAG TGCAAACCCT TCAGCAAGAA GGTGTGCTTC CTTCGGATTG GAATAATTCA AGCAATTTAA CGCGCACCAA AGACCGAAGC CATGGCGACT TTGCTTCTAA TATTGCCATG ATCGGCTCTA AAGCCGCTGG TATGAAACCA CGCGATTTAG CTGAGAAAAT TCTCGCAGCG CTACCAGAAG TTGCAGACAT CAGCAAAGCT GAAATCGCAG GCCCTGGTTT TATCAATTTC TTTTTAAATG CAGATCAACG TTTTGCAATT CTTGACCAGA TTCAGGCTCA AAAAGAAAGC TTTGGTCGTA GCCAATCAAA TGCAGCTAAA AAGATTCAAG TTGAATTCGT TTCTGCAAAC CCGACGTCTA GCCTACACGT AGGTCATGGC CGTGGTGCTG CTTATGGTAT GACTGTTGCA AACTTGCTTG AAGCAACAGG TGCTAAAGTT GATCGCGAAT ATTATGTAAA TGATGCTGGC CGTCAAATGG ATATTTTGGC GACTTCTACT TATTTACGTT ATTTGGAATT ACTTGGACAA AACCTTGTAT TCCCTAAAAA TGCATATCAA GGCGATTACG TTAAAGAAAT CGCACAAGGC ATCATTGATA AAGATGGCGA TGCTTACGTT CGTGAAGTTG CGAATGTTTA TAAAGATGTA CCAGAAGACG TGCAATATGC TGAAGAGCTA GATAGCGAAG GCAACAAAGT CGTATTATCT GGCGACAAAG AAAAACACAT TGACGGTCTT ATTGCCAACT CACAACAATT ATTAGGTGAA GGCTACCGCG TATTCCATCA AGCCGCACTC CATGCCATTT TAGATGATAT TAAAGATGAC CTTGCTGACT TCGGCGTAAC CTTTAATCAA TGGTTTAGTG AAGCATCTCT TAGCGCAAAA ATTGACGAAG CGTTAGAAAC ACTTGATCAA CGCGGCTTCT TATATGAAAA AGATGGCAAC ATCTGGTTTA AATCGACTGA ATTTGGTGAT GAAAAAGACC GTGTTGTGAA GCGCCGTAAT GGTCAAACAA CTTACTTTGC ATCTGACATT GCATATCACT TAAATAAACT ACAACGTGGT TATACTGACC TTGTTGATAT TTGGGGTTCT GACCACCACG GTTATATCTC TCGCGTAAAA GCTGCGATTG ATGCAATGGG TTATGACTCG AAAAAATTAA CTGTTCTTTT AGTTCAGTTC GTAAGCTTAT GGCGCGGTGG TGAGATGGTT CAAATGTCAT CTCGTTCAGG TCAGTTCGTG ACTTTACGTG ACTTGCGTAA AGAAGTCGGC AATGATGCAG CTCGTTTCTA CTACGTGATG CGTAAAAGTG AACAACACAT TGACTTTGAC CTTGACCTTG CTGTTTCACA AAGTAAAGAC AATGCGGTGT ATTATATTCA ATACGCTCAT GCACGTATTT GCCGTATGCT AGAAAAAGCA GCAAGCACTG GCTTACAATT TGAAGTAAGT GCTGCTCGTT CACATGCTGC ACGTTTATCA CTTGATGCAG AAACTGAAAT TCTTGCTAAA CTGGCTGCAT ATCCAGACGT TGTGTTACGT GCTGCTAATG CATATGAACC ACACCAAGTA GGTAACTACT TAAAAGAATT AGCTGCGCTA TTCCACGGTT GGTACAATGA ACACAAAGTT TTAAGTGATG ATGCTGAACT TACACAAGCG CGCTTATTAC TTTCAATCAA TGTACAGCAA GTATTGCGCA ATGGCTTAGA ATTACTCGGT GTTTCTGCAC CTGAAGCCAT GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT