AcbaC.00157.b

DNA topoisomerase IV, A subunit

CENTER ID: AcbaC.00157.b
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00157.b.B1.GE38602 full length 1 739
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3616
GenBank: AKA33370.1
PGAT: acinetobacter:ABUW_3690
RefSeq: WP_000202252.1
UniProt: X2KZV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSLAHHATE NRSVAEFTEQ AYLNYAMYVI MDRALPHISD GLKPVQRRIV YAMSELGLKS SGKPKKSART VGDVLGKYHP HGDLACYEAM VLMAQPFSYR YPLIEGQGNW GSPDDPKSFA AMRYTEAKLS AYSELLLSEL GQGTSEWQDN FDGSLKEPIT LPARVPNILL NGTTGIAVGM ATDIPPHNLR EVVKGTIALI RNPQTSDEKL AEYIPAPDLP TKAEIITPPE ELLKIQTTGR GSYRMRAVYT IEKNEIVITE LPYQVSGSKV ITQIADQMQA KKLPLVVDVR DESDHENPTR LVIVLRSNRI DAEAVMSHLF ATTDLESSYR VNLNMIGEDG RPQVKSIRRI LLEWIEIRKK TVTRRLQYHL NRIEKRLHIL AGLLIAYLDI DTVIRIIREE DQPKPVLMEH FNIDEIQAEA ILELKLRHLA KLEEMEIRHE QDELSAKAAI IREQLENPES LKNLIIGELK EDAKKFGDER RSPIVARAEA VQIKEQDLMP AETVTVVLSE AGWVRAAKGA DVDAENLNYR AGDQYLSHAV GKTNQRVYFL DETGRSYALP ISNLPSARGL GDPLSSKLSP ASGVSFIQVY LDDDESELIA ASSAGYGFKT QTKQLDTNAK AGKTFLTVPD KAKALPLISA QNMTHLAVLS SAGRLLILDL AELPNLNKGK GNKLIQLEGK EQILSMTTLN LDEIIQVVAG QQHLKLKGDD LQKYMGKRAS KGQLLPRGYQ KANKLLIQR
NT Sequence
ATGACCAGCC TTGCGCATCA TGCGACAGAA AACCGCTCTG TAGCCGAATT TACTGAACAG GCTTACTTGA ATTATGCCAT GTACGTCATT ATGGACCGTG CATTGCCGCA TATCAGTGAT GGCTTAAAGC CCGTACAGCG CCGTATTGTC TATGCTATGA GCGAGCTAGG CTTAAAAAGC AGTGGCAAGC CAAAAAAATC AGCGCGTACA GTGGGTGATG TACTTGGTAA ATACCACCCA CATGGTGACT TGGCATGTTA TGAAGCCATG GTACTCATGG CTCAGCCATT TAGTTACCGC TATCCTTTAA TCGAAGGTCA GGGGAACTGG GGTTCACCAG ATGATCCTAA ATCTTTTGCT GCGATGCGTT ATACCGAAGC CAAACTCTCG GCTTATAGTG AATTATTGCT GAGCGAATTA GGTCAGGGCA CTAGCGAATG GCAAGATAAC TTTGATGGTT CTTTAAAAGA ACCGATCACT TTACCTGCAC GTGTACCTAA TATTCTTCTT AATGGTACGA CAGGTATTGC TGTCGGGATG GCAACTGATA TCCCGCCACA TAATTTGCGT GAAGTTGTAA AAGGCACAAT TGCTTTAATC CGTAATCCGC AAACCTCGGA CGAAAAATTA GCTGAATATA TTCCAGCTCC GGATTTACCA ACCAAAGCTG AAATTATTAC CCCGCCAGAA GAATTACTCA AAATCCAGAC CACTGGTCGT GGCAGTTACC GTATGCGAGC GGTATATACC ATTGAGAAAA ATGAAATCGT AATTACTGAG CTGCCATATC AAGTCTCTGG TTCTAAGGTA ATTACTCAAA TTGCTGACCA GATGCAGGCT AAAAAGCTGC CATTAGTCGT CGATGTGCGT GATGAGTCGG ATCATGAAAA CCCGACACGA CTCGTGATTG TACTGCGCTC TAACCGTATT GATGCGGAAG CAGTGATGAG TCACTTATTT GCGACCACCG ATTTAGAATC AAGCTATCGT GTCAATTTAA ACATGATTGG CGAAGATGGC CGTCCTCAGG TGAAATCAAT TCGTCGTATT TTGCTGGAAT GGATTGAGAT CCGTAAAAAA ACGGTAACTC GTCGTTTGCA GTACCATTTA AACCGTATTG AAAAGCGCCT GCATATTTTG GCAGGTCTTT TAATTGCTTA TCTCGATATT GATACAGTCA TTCGTATTAT TCGTGAAGAA GACCAGCCTA AGCCAGTCTT GATGGAACAC TTTAATATTG ATGAGATACA GGCCGAGGCG ATTTTAGAGC TTAAATTACG TCATTTGGCA AAGCTTGAAG AGATGGAAAT TCGTCATGAA CAAGATGAGC TTTCCGCGAA AGCTGCCATT ATTCGTGAAC AACTCGAAAA TCCTGAATCT TTAAAAAATT TGATTATTGG CGAATTAAAA GAAGATGCGA AAAAGTTCGG TGATGAGCGC CGTTCTCCAA TTGTTGCACG TGCTGAAGCG GTTCAAATTA AAGAACAGGA TTTAATGCCA GCCGAAACGG TAACAGTCGT GTTGTCTGAA GCAGGCTGGG TTCGTGCGGC AAAAGGTGCG GATGTGGATG CTGAAAATCT GAACTACCGT GCTGGGGACC AATATTTAAG TCATGCTGTC GGGAAAACCA ATCAGCGAGT TTACTTCCTT GATGAAACAG GGCGCAGCTA TGCCTTGCCA ATTAGTAACT TACCTTCAGC GAGAGGCTTG GGGGATCCAT TAAGTTCTAA ATTATCACCA GCAAGTGGCG TATCGTTTAT TCAGGTTTAT TTAGATGATG ATGAGTCTGA ATTGATTGCT GCAAGTTCGG CAGGTTATGG TTTTAAAACG CAAACCAAGC AATTAGATAC CAATGCGAAA GCCGGTAAGA CATTCTTAAC GGTTCCGGAT AAGGCAAAGG CTTTACCACT CATTTCTGCC CAAAACATGA CGCATCTGGC TGTACTGAGC TCAGCAGGGC GTTTGTTAAT TTTAGATTTG GCAGAACTAC CAAATTTAAA TAAAGGTAAA GGTAATAAGT TGATACAACT TGAAGGAAAA GAGCAAATTT TATCCATGAC AACCCTGAAC TTAGATGAAA TAATTCAGGT GGTTGCAGGT CAACAACATC TCAAATTAAA AGGTGATGAT CTACAAAAAT ACATGGGTAA ACGTGCTTCG AAAGGTCAGC TCTTACCACG TGGATATCAA AAAGCAAATA AACTGTTGAT TCAGAGATAA
Details for AcbaC.00157.b.B1.GE38602
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 83kDa
OBSERVED MW: 83kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT SLAHHATENR SVAEFTEQAY LNYAMYVIMD RALPHISDGL KPVQRRIVYA MSELGLKSSG KPKKSARTVG DVLGKYHPHG DLACYEAMVL MAQPFSYRYP LIEGQGNWGS PDDPKSFAAM RYTEAKLSAY SELLLSELGQ GTSEWQDNFD GSLKEPITLP ARVPNILLNG TTGIAVGMAT DIPPHNLREV VKGTIALIRN PQTSDEKLAE YIPAPDLPTK AEIITPPEEL LKIQTTGRGS YRMRAVYTIE KNEIVITELP YQVSGSKVIT QIADQMQAKK LPLVVDVRDE SDHENPTRLV IVLRSNRIDA EAVMSHLFAT TDLESSYRVN LNMIGEDGRP QVKSIRRILL EWIEIRKKTV TRRLQYHLNR IEKRLHILAG LLIAYLDIDT VIRIIREEDQ PKPVLMEHFN IDEIQAEAIL ELKLRHLAKL EEMEIRHEQD ELSAKAAIIR EQLENPESLK NLIIGELKED AKKFGDERRS PIVARAEAVQ IKEQDLMPAE TVTVVLSEAG WVRAAKGADV DAENLNYRAG DQYLSHAVGK TNQRVYFLDE TGRSYALPIS NLPSARGLGD PLSSKLSPAS GVSFIQVYLD DDESELIAAS SAGYGFKTQT KQLDTNAKAG KTFLTVPDKA KALPLISAQN MTHLAVLSSA GRLLILDLAE LPNLNKGKGN KLIQLEGKEQ ILSMTTLNLD EIIQVVAGQQ HLKLKGDDLQ KYMGKRASKG QLLPRGYQKA NKLLIQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca gccttgcgca tcatgcgaca gaaaaccgct ctgtagccga atttactgaa caggcttact tgaattatgc catgtacgtc attatggacc gtgcattgcc gcatatcagt gatggcttaa agcccgtaca gcgccgtatt gtctatgcta tgagcgagct aggcttaaaa agcagtggca agccaaaaaa atcagcgcgt acagtgggtg atgtacttgg taaataccac ccacatggtg acttggcatg ttatgaagcc atggtactca tggctcagcc atttagttac cgctatcctt taatcgaagg tcaggggaac tggggttcac cagatgatcc taaatctttt gctgcgatgc gttataccga agccaaactc tcggcttata gtgaattatt gctgagcgaa ttaggtcagg gcactagcga atggcaagat aactttgatg gttctttaaa agaaccgatc actttacctg cacgtgtacc taatattctt cttaatggta cgacaggtat tgctgtcggg atggcaactg atatcccgcc acataatttg cgtgaagttg taaaaggcac aattgcttta atccgtaatc cgcaaacctc ggacgaaaaa ttagctgaat atattccagc tccggattta ccaaccaaag ctgaaattat taccccgcca gaagaattac tcaaaatcca gaccactggt cgtggcagtt accgtatgcg agcggtatat accattgaga aaaatgaaat cgtaattact gagctgccat atcaagtctc tggttctaag gtaattactc aaattgctga ccagatgcag gctaaaaagc tgccattagt cgtcgatgtg cgtgatgagt cggatcatga aaacccgaca cgactcgtga ttgtactgcg ctctaaccgt attgatgcgg aagcagtgat gagtcactta tttgcgacca ccgatttaga atcaagctat cgtgtcaatt taaacatgat tggcgaagat ggccgtcctc aggtgaaatc aattcgtcgt attttgctgg aatggattga gatccgtaaa aaaacggtaa ctcgtcgttt gcagtaccat ttaaaccgta ttgaaaagcg cctgcatatt ttggcaggtc ttttaattgc ttatctcgat attgatacag tcattcgtat tattcgtgaa gaagaccagc ctaagccagt cttgatggaa cactttaata ttgatgagat acaggccgag gcgattttag agcttaaatt acgtcatttg gcaaagcttg aagagatgga aattcgtcat gaacaagatg agctttccgc gaaagctgcc attattcgtg aacaactcga aaatcctgaa tctttaaaaa atttgattat tggcgaatta aaagaagatg cgaaaaagtt cggtgatgag cgccgttctc caattgttgc acgtgctgaa gcggttcaaa ttaaagaaca ggatttaatg ccagccgaaa cggtaacagt cgtgttgtct gaagcaggct gggttcgtgc ggcaaaaggt gcggatgtgg atgctgaaaa tctgaactac cgtgctgggg accaatattt aagtcatgct gtcgggaaaa ccaatcagcg agtttacttc cttgatgaaa cagggcgcag ctatgccttg ccaattagta acttaccttc agcgagaggc ttgggggatc cattaagttc taaattatca ccagcaagtg gcgtatcgtt tattcaggtt tatttagatg atgatgagtc tgaattgatt gctgcaagtt cggcaggtta tggttttaaa acgcaaacca agcaattaga taccaatgcg aaagccggta agacattctt aacggttccg gataaggcaa aggctttacc actcatttct gcccaaaaca tgacgcatct ggctgtactg agctcagcag ggcgtttgtt aattttagat ttggcagaac taccaaattt aaataaaggt aaaggtaata agttgataca acttgaagga aaagagcaaa ttttatccat gacaaccctg aacttagatg aaataattca ggtggttgca ggtcaacaac atctcaaatt aaaaggtgat gatctacaaa aatacatggg taaacgtgct tcgaaaggtc agctcttacc acgtggatat caaaaagcaa ataaactgtt gattcagaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat