AcbaC.00157.a

DNA gyrase, A subunit

CENTER ID: AcbaC.00157.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00157.a.B1.GE38601 full length 1 904
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.924
GenBank: AKA30714.1
PGAT: acinetobacter:ABUW_0960
RefSeq: WP_000116445.1
UniProt: A0A0D5YFF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVSEIRPIA IEDELKHSYL DYAMSVIVSR ALPDVRDGLK PVHRRVLYAM HELGNDYNKA YKKSARVVGD VIGKYHPHGD LAVYETIVRM AQDFSLRYLL VDGQGNFGSI DGDSAAAMRY TEVRMTKLAH ELLADLEKDT VDWEDNYDGS ERIPEVLPTR VPNLLINGAA GIAVGMATNM APHNMTEVVN ACLAYADNPN ISIEGLMEYI TGPDFPTGGI IYGKSGIVDA YRTGKGRLHI RGKYHFEEDE KTGRTTIVFT EIPYQVNKAR VIERIAELVK EKKLEGISEL RDESDKEGMR IAIDLKRGEN AEVVVNNLFL NTQLENSFSI NMVCLDNGQP KLMNLKDIIA AFIRHRQEVV TRRTMFELRK ARERGHILEG LTVALANIDE IIETIKTSAN PAEARERLLA GEWAGGGVVA LLEKAGAISV RPDEIEGEDP NRPFGLSDSI YRLSPTQVGA ILELRLHRLT GLEQDKLHAE YTEILGQIAE LTAILNDFNL LMGVIREELA QVLQQYGDAR RTEIVESRVD FCREDLIPEE QVVLTVSQTG YAKTQPLSDY QAQRRGGRGK SATSMKDDDF IQHLIVASNH ATVLCFTNVG KVYRLKVFEV PQASRGAKGR PIVNLLPLDA TETVTAILPL TEFPENHYVF MATASGTVKR VELEQFANIR SNGLRAIELN EEDTLIGVAI TDGNQQIMLF SNEGKAIRFA ETDVRAMGRT AKGVRGMRVS FASSTLSEED ADVENDDSDD NDDSTDSSLV SRIVSLVVVP ETGEVLCASA NGYGKRTPVN DFPTKKRGGK GVIAIKTSER NGELVGAVSI DETKELLLIS DGGTLVRTRA AEVAMTGRNA QGVRLIRLSE EETLVGVVSI EAVEDEEELL EGEVDTTETD SEEAVSNNED TSEE
NT Sequence
ATGAGCGTAT CGGAAATCCG ACCGATTGCC ATTGAGGACG AACTCAAGCA TTCATATTTA GATTACGCGA TGAGTGTAAT TGTATCTCGT GCATTGCCGG ATGTGAGAGA CGGTCTTAAA CCTGTTCACC GTCGTGTGCT TTATGCCATG CACGAATTGG GCAATGACTA TAACAAAGCC TACAAGAAAT CTGCTCGTGT CGTTGGGGAC GTAATCGGTA AATATCACCC GCATGGTGAC TTAGCTGTTT ATGAAACCAT TGTTCGTATG GCTCAAGACT TTAGCTTACG TTATTTATTG GTTGATGGTC AGGGTAACTT CGGTTCGATC GATGGTGATA GCGCTGCGGC AATGCGTTAT ACCGAAGTCC GTATGACTAA GCTGGCACAT GAGCTTCTTG CAGATTTAGA AAAAGACACA GTTGACTGGG AAGATAACTA CGACGGTTCG GAACGTATCC CTGAAGTACT TCCGACACGT GTTCCAAACT TATTAATTAA CGGTGCTGCT GGTATTGCTG TAGGTATGGC AACTAACATG GCACCACACA ACATGACAGA AGTTGTGAAT GCTTGTTTGG CTTATGCTGA CAATCCGAAT ATCTCGATTG AAGGATTGAT GGAATACATT ACTGGTCCTG ACTTCCCTAC AGGCGGTATT ATTTACGGTA AATCAGGTAT TGTTGATGCC TACCGTACCG GTAAAGGTCG TTTACACATT CGTGGTAAAT ACCATTTCGA AGAAGATGAA AAGACAGGTC GTACAACCAT CGTCTTTACT GAAATTCCAT ATCAAGTAAA CAAAGCAAGA GTTATTGAAC GTATTGCCGA GTTAGTAAAA GAGAAAAAGC TTGAAGGTAT TTCAGAACTT CGTGATGAGT CTGATAAAGA AGGTATGCGT ATTGCAATTG ACTTGAAACG CGGTGAAAAC GCAGAAGTCG TTGTAAATAA CTTATTCTTA AATACCCAGC TTGAAAACTC ATTCAGCATC AACATGGTTT GTCTAGACAA TGGACAACCA AAATTGATGA ATCTAAAAGA TATTATTGCG GCATTTATTC GTCACCGCCA AGAAGTTGTG ACACGCCGTA CCATGTTCGA ATTACGTAAA GCACGTGAAC GTGGTCATAT CTTGGAAGGC TTGACAGTTG CCTTAGCCAA TATTGATGAA ATTATTGAAA CCATCAAAAC TTCTGCAAAC CCTGCTGAAG CGCGTGAGCG TTTACTTGCG GGTGAGTGGG CAGGTGGTGG CGTTGTTGCA CTACTTGAAA AAGCTGGTGC AATTTCTGTT CGCCCAGATG AAATTGAAGG TGAAGATCCA AATCGTCCAT TTGGTTTAAG TGATTCAATT TATCGTCTGT CACCAACACA AGTAGGCGCA ATTTTAGAAT TACGTTTACA CCGTTTAACT GGTCTTGAAC AAGACAAGTT ACATGCGGAA TATACTGAAA TTTTAGGTCA AATTGCTGAA CTTACTGCAA TTTTAAATGA CTTTAACTTG TTAATGGGTG TTATTCGCGA AGAGTTGGCA CAAGTTTTAC AACAATATGG CGATGCACGT CGTACCGAAA TTGTTGAATC TCGTGTGGAT TTCTGCCGTG AAGATTTAAT TCCTGAAGAG CAAGTGGTAT TAACGGTTTC GCAAACGGGT TATGCAAAAA CTCAACCTCT TTCAGACTAT CAGGCACAGC GCCGTGGTGG ACGTGGTAAG TCTGCAACCT CAATGAAAGA TGATGACTTT ATTCAACATC TGATTGTGGC ATCGAACCAT GCGACCGTAC TTTGCTTTAC CAATGTGGGT AAAGTGTACC GTCTGAAAGT ATTTGAAGTT CCTCAAGCAT CACGTGGGGC AAAAGGCCGT CCAATCGTGA ACTTGTTACC TCTAGATGCA ACAGAAACCG TAACCGCAAT CTTGCCGTTA ACCGAGTTCC CGGAAAACCA CTATGTGTTT ATGGCGACAG CTTCTGGTAC GGTTAAGCGT GTTGAGTTAG AGCAATTTGC AAACATTCGT TCAAATGGTC TACGTGCTAT TGAACTTAAT GAAGAAGATA CTTTAATTGG TGTTGCGATT ACTGATGGTA ATCAGCAAAT CATGTTGTTC TCTAACGAAG GTAAGGCAAT TCGTTTTGCT GAAACTGACG TACGTGCAAT GGGTCGTACA GCGAAAGGTG TACGCGGTAT GCGCGTGAGT TTTGCAAGCA GCACCTTAAG TGAAGAAGAT GCAGATGTTG AAAATGATGA TTCAGATGAT AATGATGATT CAACAGATTC AAGTCTAGTA AGTCGCATCG TATCGCTTGT TGTTGTACCT GAGACAGGCG AAGTACTGTG TGCGAGTGCC AACGGTTATG GTAAACGTAC TCCAGTAAAT GACTTCCCGA CCAAGAAACG TGGTGGTAAG GGTGTTATTG CGATCAAGAC AAGTGAACGT AACGGTGAGC TAGTTGGTGC AGTTTCTATT GATGAAACCA AAGAGTTATT ATTAATTTCT GATGGTGGTA CGCTTGTTCG TACGCGTGCT GCAGAAGTTG CAATGACAGG CCGTAATGCT CAAGGTGTTC GTCTGATCCG TTTAAGCGAA GAAGAAACGC TCGTTGGCGT AGTTTCAATT GAAGCTGTAG AAGACGAAGA AGAACTTCTT GAAGGTGAAG TAGATACGAC TGAAACTGAT AGCGAAGAAG CTGTATCTAA TAATGAAGAT ACTTCTGAAG AGTAA
Details for AcbaC.00157.a.B1.GE38601
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 101kDa
OBSERVED MW: 101kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS VSEIRPIAIE DELKHSYLDY AMSVIVSRAL PDVRDGLKPV HRRVLYAMHE LGNDYNKAYK KSARVVGDVI GKYHPHGDLA VYETIVRMAQ DFSLRYLLVD GQGNFGSIDG DSAAAMRYTE VRMTKLAHEL LADLEKDTVD WEDNYDGSER IPEVLPTRVP NLLINGAAGI AVGMATNMAP HNMTEVVNAC LAYADNPNIS IEGLMEYITG PDFPTGGIIY GKSGIVDAYR TGKGRLHIRG KYHFEEDEKT GRTTIVFTEI PYQVNKARVI ERIAELVKEK KLEGISELRD ESDKEGMRIA IDLKRGENAE VVVNNLFLNT QLENSFSINM VCLDNGQPKL MNLKDIIAAF IRHRQEVVTR RTMFELRKAR ERGHILEGLT VALANIDEII ETIKTSANPA EARERLLAGE WAGGGVVALL EKAGAISVRP DEIEGEDPNR PFGLSDSIYR LSPTQVGAIL ELRLHRLTGL EQDKLHAEYT EILGQIAELT AILNDFNLLM GVIREELAQV LQQYGDARRT EIVESRVDFC REDLIPEEQV VLTVSQTGYA KTQPLSDYQA QRRGGRGKSA TSMKDDDFIQ HLIVASNHAT VLCFTNVGKV YRLKVFEVPQ ASRGAKGRPI VNLLPLDATE TVTAILPLTE FPENHYVFMA TASGTVKRVE LEQFANIRSN GLRAIELNEE DTLIGVAITD GNQQIMLFSN EGKAIRFAET DVRAMGRTAK GVRGMRVSFA SSTLSEEDAD VENDDSDDND DSTDSSLVSR IVSLVVVPET GEVLCASANG YGKRTPVNDF PTKKRGGKGV IAIKTSERNG ELVGAVSIDE TKELLLISDG GTLVRTRAAE VAMTGRNAQG VRLIRLSEEE TLVGVVSIEA VEDEEELLEG EVDTTETDSE EAVSNNEDTS EE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tatcggaaat ccgaccgatt gccattgagg acgaactcaa gcattcatat ttagattacg cgatgagtgt aattgtatct cgtgcattgc cggatgtgag agacggtctt aaacctgttc accgtcgtgt gctttatgcc atgcacgaat tgggcaatga ctataacaaa gcctacaaga aatctgctcg tgtcgttggg gacgtaatcg gtaaatatca cccgcatggt gacttagctg tttatgaaac cattgttcgt atggctcaag actttagctt acgttattta ttggttgatg gtcagggtaa cttcggttcg atcgatggtg atagcgctgc ggcaatgcgt tataccgaag tccgtatgac taagctggca catgagcttc ttgcagattt agaaaaagac acagttgact gggaagataa ctacgacggt tcggaacgta tccctgaagt acttccgaca cgtgttccaa acttattaat taacggtgct gctggtattg ctgtaggtat ggcaactaac atggcaccac acaacatgac agaagttgtg aatgcttgtt tggcttatgc tgacaatccg aatatctcga ttgaaggatt gatggaatac attactggtc ctgacttccc tacaggcggt attatttacg gtaaatcagg tattgttgat gcctaccgta ccggtaaagg tcgtttacac attcgtggta aataccattt cgaagaagat gaaaagacag gtcgtacaac catcgtcttt actgaaattc catatcaagt aaacaaagca agagttattg aacgtattgc cgagttagta aaagagaaaa agcttgaagg tatttcagaa cttcgtgatg agtctgataa agaaggtatg cgtattgcaa ttgacttgaa acgcggtgaa aacgcagaag tcgttgtaaa taacttattc ttaaataccc agcttgaaaa ctcattcagc atcaacatgg tttgtctaga caatggacaa ccaaaattga tgaatctaaa agatattatt gcggcattta ttcgtcaccg ccaagaagtt gtgacacgcc gtaccatgtt cgaattacgt aaagcacgtg aacgtggtca tatcttggaa ggcttgacag ttgccttagc caatattgat gaaattattg aaaccatcaa aacttctgca aaccctgctg aagcgcgtga gcgtttactt gcgggtgagt gggcaggtgg tggcgttgtt gcactacttg aaaaagctgg tgcaatttct gttcgcccag atgaaattga aggtgaagat ccaaatcgtc catttggttt aagtgattca atttatcgtc tgtcaccaac acaagtaggc gcaattttag aattacgttt acaccgttta actggtcttg aacaagacaa gttacatgcg gaatatactg aaattttagg tcaaattgct gaacttactg caattttaaa tgactttaac ttgttaatgg gtgttattcg cgaagagttg gcacaagttt tacaacaata tggcgatgca cgtcgtaccg aaattgttga atctcgtgtg gatttctgcc gtgaagattt aattcctgaa gagcaagtgg tattaacggt ttcgcaaacg ggttatgcaa aaactcaacc tctttcagac tatcaggcac agcgccgtgg tggacgtggt aagtctgcaa cctcaatgaa agatgatgac tttattcaac atctgattgt ggcatcgaac catgcgaccg tactttgctt taccaatgtg ggtaaagtgt accgtctgaa agtatttgaa gttcctcaag catcacgtgg ggcaaaaggc cgtccaatcg tgaacttgtt acctctagat gcaacagaaa ccgtaaccgc aatcttgccg ttaaccgagt tcccggaaaa ccactatgtg tttatggcga cagcttctgg tacggttaag cgtgttgagt tagagcaatt tgcaaacatt cgttcaaatg gtctacgtgc tattgaactt aatgaagaag atactttaat tggtgttgcg attactgatg gtaatcagca aatcatgttg ttctctaacg aaggtaaggc aattcgtttt gctgaaactg acgtacgtgc aatgggtcgt acagcgaaag gtgtacgcgg tatgcgcgtg agttttgcaa gcagcacctt aagtgaagaa gatgcagatg ttgaaaatga tgattcagat gataatgatg attcaacaga ttcaagtcta gtaagtcgca tcgtatcgct tgttgttgta cctgagacag gcgaagtact gtgtgcgagt gccaacggtt atggtaaacg tactccagta aatgacttcc cgaccaagaa acgtggtggt aagggtgtta ttgcgatcaa gacaagtgaa cgtaacggtg agctagttgg tgcagtttct attgatgaaa ccaaagagtt attattaatt tctgatggtg gtacgcttgt tcgtacgcgt gctgcagaag ttgcaatgac aggccgtaat gctcaaggtg ttcgtctgat ccgtttaagc gaagaagaaa cgctcgttgg cgtagtttca attgaagctg tagaagacga agaagaactt cttgaaggtg aagtagatac gactgaaact gatagcgaag aagctgtatc taataatgaa gatacttctg aagagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat