AcbaC.00155.a

ATP-dependent DNA helicase

CENTER ID: AcbaC.00155.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00155.a.B1.GE38599 full length 1 679
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2994
GenBank: AKA32756.1
PGAT: acinetobacter:ABUW_3043
RefSeq: WP_000093035.1
UniProt: V5VG28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSASQLNNK QEEAMKYTQG PLLVLAGAGS GKTSVITRKI AYLVQHCRIP AHRITAMTFT NKAAREMKER VTKLLSREEA KGLSVSTFHT FGLNLLRLEL KNLPLKANFS ILDADDCKRI LMDLMHRDNL SGAESKELIA KAMKKISDWK NDLILPEQAH STCETPEDVQ FAHLYQLYER NLRAYNAVDF DDLIVMPTRL LQENAEVRDK WQNRVRYLLV DEYQDTNTAQ YILVKLLVGV MGQFTAVGDD DQSIYAWRGA KPENMALLKQ DFPNLHIIKL EQNYRSTSRI LKAANCVIQN NPHIFDKKLW SDKGHGEVIR IITCRNDDDE AERVVKDLLT HKLMNGKNWK DYAVLYRGNF QARVLETQLR QMQIPYKLSG GTSFFARAEI KDVMSYLRLI INPEDDSAFL RIINTPKRAI GPVTLEKLGL FAQENNLSLL GASSDQRLSM VLPKKAETQL HEFADFISTF TRELLEDDEP VPKVRQMMNE AGYIDYIREQ SATPAQEKSK LDNIENLFSS IQNLINRAED VDEKNIESVI RKLVLLDMLE QQQEEEDTDK VNLLTLHAAK GLEFPYVYIM GLEEELLPHK NSIAAETIEE ERRLMYVGIT RARQGLTLTL AEQRKNGGQM KQMTPSRFLD ELPQDELEWL GRKKKIAANV DPKEQAQQYL ANLKALLKR
NT Sequence
ATGTCATCTG CCAGTCAACT GAACAACAAG CAAGAAGAAG CCATGAAATA TACTCAGGGC CCTTTATTGG TGCTTGCTGG CGCTGGTTCA GGTAAAACAT CGGTAATTAC GCGTAAAATT GCCTATTTGG TGCAACATTG CCGTATTCCT GCGCATCGTA TTACAGCGAT GACCTTTACC AATAAAGCTG CACGTGAAAT GAAAGAGCGT GTCACTAAGC TTTTATCACG TGAAGAAGCA AAAGGTTTGT CCGTTTCGAC CTTCCATACT TTTGGTTTAA ATCTTTTACG TTTAGAACTT AAAAATTTAC CTTTAAAAGC TAATTTTTCG ATTTTGGATG CAGATGACTG TAAACGTATT TTGATGGATT TGATGCATCG CGATAATTTA TCGGGTGCAG AGAGTAAAGA ACTAATTGCC AAGGCAATGA AGAAAATTTC AGATTGGAAA AACGATCTGA TTTTGCCTGA ACAAGCACAT TCGACGTGCG AAACTCCTGA AGATGTCCAG TTTGCCCATC TTTATCAACT TTATGAACGT AATTTACGTG CCTACAATGC AGTTGATTTT GATGACCTCA TTGTTATGCC TACCCGCTTA TTGCAAGAAA ATGCAGAAGT ACGTGACAAA TGGCAAAACC GTGTTCGTTA CCTGCTAGTG GATGAGTATC AAGATACCAA CACCGCGCAA TATATTTTGG TGAAATTACT AGTTGGCGTA ATGGGGCAGT TTACTGCCGT AGGTGATGAT GACCAATCGA TTTATGCATG GCGTGGGGCT AAGCCTGAAA ACATGGCTTT GCTTAAACAG GATTTCCCAA ATCTACATAT TATTAAACTT GAGCAAAATT ACCGCTCAAC CAGCCGTATT TTGAAAGCTG CGAACTGTGT AATTCAAAAT AACCCGCATA TTTTTGATAA AAAATTGTGG AGTGATAAAG GCCATGGTGA GGTCATCAGA ATTATTACCT GTCGTAATGA TGATGATGAA GCAGAACGTG TAGTCAAAGA CTTACTGACT CATAAACTTA TGAACGGTAA GAACTGGAAA GATTACGCTG TATTGTACCG TGGTAACTTC CAGGCACGTG TATTAGAAAC ACAACTTCGC CAAATGCAGA TTCCGTACAA ACTCTCTGGC GGTACATCGT TCTTCGCTCG TGCAGAAATT AAAGATGTAA TGAGTTATTT ACGTCTTATT ATTAACCCAG AAGATGACAG TGCTTTCTTA CGTATTATTA ATACGCCTAA ACGTGCGATT GGCCCAGTTA CTCTTGAGAA GTTAGGCTTA TTTGCACAAG AAAATAATTT GTCTTTACTT GGTGCTTCTT CTGACCAGCG CTTAAGTATG GTGCTTCCTA AAAAGGCAGA AACTCAATTG CATGAGTTTG CTGACTTTAT CTCGACGTTT ACACGTGAGT TGTTGGAAGA TGATGAGCCT GTGCCTAAAG TTCGTCAAAT GATGAATGAA GCGGGTTATA TCGACTATAT TCGTGAGCAG TCCGCAACGC CTGCTCAAGA GAAAAGTAAG CTCGATAATA TCGAGAACTT GTTTAGCAGT ATTCAAAACT TAATTAATCG TGCTGAAGAT GTTGATGAAA AGAACATTGA AAGTGTGATT CGAAAACTAG TCTTGCTTGA TATGTTAGAG CAACAGCAAG AGGAAGAAGA CACTGATAAA GTGAACTTAC TTACTCTACA TGCCGCTAAA GGTTTGGAAT TCCCATATGT CTATATTATG GGTCTTGAAG AAGAGCTGTT GCCGCATAAG AACTCGATTG CTGCTGAAAC AATTGAAGAA GAACGCCGTC TTATGTACGT GGGAATTACC CGTGCACGTC AAGGCTTAAC TTTGACTTTG GCAGAGCAGC GTAAAAATGG TGGTCAAATG AAACAGATGA CTCCAAGCCG TTTCCTCGAT GAGCTTCCGC AAGATGAGCT CGAATGGTTA GGGCGTAAAA AGAAAATTGC AGCAAATGTT GATCCTAAAG AACAGGCTCA ACAATATTTG GCAAATTTAA AAGCTTTATT AAAGCGTTAA
Details for AcbaC.00155.a.B1.GE38599
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 79kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SASQLNNKQE EAMKYTQGPL LVLAGAGSGK TSVITRKIAY LVQHCRIPAH RITAMTFTNK AAREMKERVT KLLSREEAKG LSVSTFHTFG LNLLRLELKN LPLKANFSIL DADDCKRILM DLMHRDNLSG AESKELIAKA MKKISDWKND LILPEQAHST CETPEDVQFA HLYQLYERNL RAYNAVDFDD LIVMPTRLLQ ENAEVRDKWQ NRVRYLLVDE YQDTNTAQYI LVKLLVGVMG QFTAVGDDDQ SIYAWRGAKP ENMALLKQDF PNLHIIKLEQ NYRSTSRILK AANCVIQNNP HIFDKKLWSD KGHGEVIRII TCRNDDDEAE RVVKDLLTHK LMNGKNWKDY AVLYRGNFQA RVLETQLRQM QIPYKLSGGT SFFARAEIKD VMSYLRLIIN PEDDSAFLRI INTPKRAIGP VTLEKLGLFA QENNLSLLGA SSDQRLSMVL PKKAETQLHE FADFISTFTR ELLEDDEPVP KVRQMMNEAG YIDYIREQSA TPAQEKSKLD NIENLFSSIQ NLINRAEDVD EKNIESVIRK LVLLDMLEQQ QEEEDTDKVN LLTLHAAKGL EFPYVYIMGL EEELLPHKNS IAAETIEEER RLMYVGITRA RQGLTLTLAE QRKNGGQMKQ MTPSRFLDEL PQDELEWLGR KKKIAANVDP KEQAQQYLAN LKALLKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcat ctgccagtca actgaacaac aagcaagaag aagccatgaa atatactcag ggccctttat tggtgcttgc tggcgctggt tcaggtaaaa catcggtaat tacgcgtaaa attgcctatt tggtgcaaca ttgccgtatt cctgcgcatc gtattacagc gatgaccttt accaataaag ctgcacgtga aatgaaagag cgtgtcacta agcttttatc acgtgaagaa gcaaaaggtt tgtccgtttc gaccttccat acttttggtt taaatctttt acgtttagaa cttaaaaatt tacctttaaa agctaatttt tcgattttgg atgcagatga ctgtaaacgt attttgatgg atttgatgca tcgcgataat ttatcgggtg cagagagtaa agaactaatt gccaaggcaa tgaagaaaat ttcagattgg aaaaacgatc tgattttgcc tgaacaagca cattcgacgt gcgaaactcc tgaagatgtc cagtttgccc atctttatca actttatgaa cgtaatttac gtgcctacaa tgcagttgat tttgatgacc tcattgttat gcctacccgc ttattgcaag aaaatgcaga agtacgtgac aaatggcaaa accgtgttcg ttacctgcta gtggatgagt atcaagatac caacaccgcg caatatattt tggtgaaatt actagttggc gtaatggggc agtttactgc cgtaggtgat gatgaccaat cgatttatgc atggcgtggg gctaagcctg aaaacatggc tttgcttaaa caggatttcc caaatctaca tattattaaa cttgagcaaa attaccgctc aaccagccgt attttgaaag ctgcgaactg tgtaattcaa aataacccgc atatttttga taaaaaattg tggagtgata aaggccatgg tgaggtcatc agaattatta cctgtcgtaa tgatgatgat gaagcagaac gtgtagtcaa agacttactg actcataaac ttatgaacgg taagaactgg aaagattacg ctgtattgta ccgtggtaac ttccaggcac gtgtattaga aacacaactt cgccaaatgc agattccgta caaactctct ggcggtacat cgttcttcgc tcgtgcagaa attaaagatg taatgagtta tttacgtctt attattaacc cagaagatga cagtgctttc ttacgtatta ttaatacgcc taaacgtgcg attggcccag ttactcttga gaagttaggc ttatttgcac aagaaaataa tttgtcttta cttggtgctt cttctgacca gcgcttaagt atggtgcttc ctaaaaaggc agaaactcaa ttgcatgagt ttgctgactt tatctcgacg tttacacgtg agttgttgga agatgatgag cctgtgccta aagttcgtca aatgatgaat gaagcgggtt atatcgacta tattcgtgag cagtccgcaa cgcctgctca agagaaaagt aagctcgata atatcgagaa cttgtttagc agtattcaaa acttaattaa tcgtgctgaa gatgttgatg aaaagaacat tgaaagtgtg attcgaaaac tagtcttgct tgatatgtta gagcaacagc aagaggaaga agacactgat aaagtgaact tacttactct acatgccgct aaaggtttgg aattcccata tgtctatatt atgggtcttg aagaagagct gttgccgcat aagaactcga ttgctgctga aacaattgaa gaagaacgcc gtcttatgta cgtgggaatt acccgtgcac gtcaaggctt aactttgact ttggcagagc agcgtaaaaa tggtggtcaa atgaaacaga tgactccaag ccgtttcctc gatgagcttc cgcaagatga gctcgaatgg ttagggcgta aaaagaaaat tgcagcaaat gttgatccta aagaacaggc tcaacaatat ttggcaaatt taaaagcttt attaaagcgt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat