AcbaC.00150.a
UDP-N-acetylglucosamine diphosphorylase/glucosamine-1- phosphate N-acetyltransferase
| CENTER ID: | AcbaC.00150.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00150.a.B1.GE38598 | full length | 1 | 454 | ||
| AcbaC.00150.a.B3.GE40743 | truncation 2 | 1 | 450 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00150.a.B3.PS38180 | truncation 2 | 1 | 450 | ||
| AcbaC.00150.a.B1.PW37634 | full length | 1 | 454 |
Structures
5VMK
DEPOSITED: 4/27/2017
DETERMINATION: XRay
CLONE: AcbaC.00150.a.B1.GE38598
PROTEIN: AcbaC.00150.a.B1.PW37634
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA29885.1 |
| PATRIC ID: | fig|470.1345.peg.86 |
| PGAT: | acinetobacter:ABUW_0090 |
| RefSeq: | WP_000108585.1 |
| UniProt: | A0A0D5YCC7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTTVIILAA GKGTRMRSQL PKVLQPLAGR PLLGHVIKTA KQLLAENIIT IYGHGGDHVK KTFAQENIQW VEQAEQLGTG HAVQMTLPVL PKDGISLILY GDVPLVRQTT LEQLIEVSNK TGIGMITLHV DNPTGYGRIV RQDGKIQAIV EHKDATEAQR QIQEINTGIY CVSNAKLHEW LPKLSNENAQ GEYYLTDIVA MAVADGLEIA SIQPELAFEV EGVNDRLQLA ALEREFQKQQ AKELMQQGVT FADPARFDLR GTVKVGHDVR IDVNVIIEGN CELGDFVEIG AGCILKNTTI AAGTKVQAYS VFDGAVVGEN TQIGPFARLR PGAKLANEVH IGNFVEVKNT TIGLGSKANH FTYLGDAEIG AESNIGAGTI TCNYDGANKH KTTIGDAVFI GSNSSLVAPV TIGNGATVGA GSVITKDVAE QSLSFERAQQ ISKANYQRPQ KLKK
NT Sequence
ATGTCAACTA CTGTCATTAT TCTTGCTGCA GGCAAAGGAA CGCGAATGCG TTCTCAGCTC CCTAAAGTAC TACAACCTTT AGCTGGTCGC CCGCTACTAG GACATGTTAT TAAAACAGCA AAACAACTAT TAGCAGAAAA CATCATTACC ATTTATGGAC ATGGTGGCGA TCACGTAAAA AAGACATTCG CACAAGAAAA TATTCAGTGG GTTGAACAAG CAGAACAGTT AGGGACCGGT CATGCGGTAC AGATGACTTT ACCGGTACTT CCTAAAGACG GCATTTCATT AATTTTATAC GGTGATGTTC CTTTAGTCCG TCAAACAACG CTTGAACAAC TGATTGAAGT ATCAAATAAA ACCGGTATTG GCATGATTAC TTTACATGTC GATAACCCAA CAGGTTATGG CCGTATTGTC CGCCAAGACG GTAAAATCCA GGCGATTGTT GAACATAAAG ACGCAACCGA AGCTCAGCGT CAAATTCAAG AAATTAACAC AGGTATTTAC TGTGTCAGCA ATGCAAAACT TCATGAGTGG TTGCCTAAGC TTTCAAATGA AAATGCACAA GGCGAATACT ATTTAACTGA TATCGTTGCG ATGGCTGTTG CTGATGGTCT AGAAATCGCT TCAATTCAAC CAGAGCTCGC ATTTGAAGTT GAAGGTGTAA ATGACCGCCT GCAACTTGCA GCACTTGAGC GGGAATTTCA GAAGCAACAA GCAAAAGAAC TTATGCAGCA AGGCGTAACT TTTGCTGATC CTGCGCGTTT TGATTTACGT GGTACAGTAA AAGTTGGTCA TGATGTTCGT ATTGATGTGA ATGTCATTAT TGAAGGTAAC TGTGAACTCG GTGACTTTGT CGAAATCGGT GCTGGCTGTA TCTTAAAAAA TACAACCATT GCAGCGGGCA CTAAAGTCCA AGCGTACAGC GTTTTTGATG GAGCAGTTGT TGGGGAAAAT ACCCAAATTG GTCCATTCGC ACGCTTACGT CCAGGTGCTA AATTGGCAAA CGAAGTACAT ATCGGCAACT TTGTTGAAGT CAAAAATACA ACGATCGGTC TAGGCAGTAA AGCTAACCAT TTCACCTATT TAGGTGATGC CGAGATCGGT GCTGAATCAA ATATTGGGGC AGGTACAATC ACCTGCAATT ATGATGGTGC AAATAAACAC AAAACCACTA TTGGCGATGC AGTTTTTATC GGTTCTAATA GCTCGCTGGT TGCGCCTGTG ACTATTGGTA ATGGAGCAAC AGTCGGTGCG GGTTCTGTGA TTACGAAAGA TGTGGCTGAA CAAAGTCTCT CCTTTGAGCG TGCTCAACAA ATTTCTAAAG CAAATTATCA ACGTCCCCAA AAGCTTAAAA AATAA
Details for AcbaC.00150.a.B1.GE38598
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS KANY
Validated NT Sequence
gataatttgc tttagaaatt tgttgagcac gctcaaagga gagactttgt tcagccacat ctttcgtaat cacagaaccc gcaccgactg ttgctccatt accaatagtc acaggcgcaa ccagcgagct attagaaccg ataaaaactg catcgccaat agtggttttg tgtttatttg caccatcata attgcaggtg attgtacctg ccccaatatt tgattcagca ccgatctcgg catcacctaa ataggtgaaa tggttagctt tactgcctag accgatcgtt gtatttttga cttcaacaaa gttgccgata tgtacttcgt ttgccaattt agcacctgga cgtaagcgtg cgaatggacc aatttgggta ttttccccaa caactgctcc atcaaaaacg ctgtacgctt ggactttagt gcccgctgca atggttgtat tttttaagat acagccagca ccgatttcga caaagtcacc gagttcacag ttaccttcaa taatgacatt cacatcaata cgaacatcat gaccaacttt tactgtacca cgtaaatcaa aacgcgcagg atcagcaaaa gttacgcctt gctgcataag ttcttttgct tgttgcttct gaaattcccg ctcaagtgct gcaagttgca ggcggtcatt tacaccttca acttcaaatg cgagctctgg ttgaattgaa gcgatttcta gaccatcagc aacagccatc gcaacgatat cagttaaata gtattcgcct tgtgcatttt catttgaaag cttaggcaac cactcatgaa gttttgcatt gctgacacag taaatacctg tgttaatttc ttgaatttga cgctgagctt cggttgcgtc tttatgttca acaatcgcct ggattttacc gtcttggcgg acaatacggc cataacctgt tgggttatcg acatgtaaag taatcatgcc aataccggtt ttatttgata cttcaatcag ttgttcaagc gttgtttgac ggactaaagg aacatcaccg tataaaatta atgaaatgcc gtctttagga agtaccggta aagtcatctg taccgcatga ccggtcccta actgttctgc ttgttcaacc cactgaatat tttcttgtgc gaatgtcttt tttacgtgat cgccaccatg tccataaatg gtaatgatgt tttctgctaa tagttgtttt gctgttttaa taacatgtcc tagtagcggg cgaccagcta aaggttgtag tactttaggg agctgagaac gcattcgcgt tcctttgcct gcagcaagaa taatgacagt agttgacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS KANYQRPQKL KK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa ctactgtcat tattcttgct gcaggcaaag gaacgcgaat gcgttctcag ctccctaaag tactacaacc tttagctggt cgcccgctac taggacatgt tattaaaaca gcaaaacaac tattagcaga aaacatcatt accatttatg gacatggtgg cgatcacgta aaaaagacat tcgcacaaga aaatattcag tgggttgaac aagcagaaca gttagggacc ggtcatgcgg tacagatgac tttaccggta cttcctaaag acggcatttc attaatttta tacggtgatg ttcctttagt ccgtcaaaca acgcttgaac aactgattga agtatcaaat aaaaccggta ttggcatgat tactttacat gtcgataacc caacaggtta tggccgtatt gtccgccaag acggtaaaat ccaggcgatt gttgaacata aagacgcaac cgaagctcag cgtcaaattc aagaaattaa cacaggtatt tactgtgtca gcaatgcaaa acttcatgag tggttgccta agctttcaaa tgaaaatgca caaggcgaat actatttaac tgatatcgtt gcgatggctg ttgctgatgg tctagaaatc gcttcaattc aaccagagct cgcatttgaa gttgaaggtg taaatgaccg cctgcaactt gcagcacttg agcgggaatt tcagaagcaa caagcaaaag aacttatgca gcaaggcgta acttttgctg atcctgcgcg ttttgattta cgtggtacag taaaagttgg tcatgatgtt cgtattgatg tgaatgtcat tattgaaggt aactgtgaac tcggtgactt tgtcgaaatc ggtgctggct gtatcttaaa aaatacaacc attgcagcgg gcactaaagt ccaagcgtac agcgtttttg atggagcagt tgttggggaa aatacccaaa ttggtccatt cgcacgctta cgtccaggtg ctaaattggc aaacgaagta catatcggca actttgttga agtcaaaaat acaacgatcg gtctaggcag taaagctaac catttcacct atttaggtga tgccgagatc ggtgctgaat caaatattgg ggcaggtaca atcacctgca attatgatgg tgcaaataaa cacaaaacca ctattggcga tgcagttttt atcggttcta atagctcgct ggttgcgcct gtgactattg gtaatggagc aacagtcggt gcgggttctg tgattacgaa agatgtggct gaacaaagtc tctcctttga gcgtgctcaa caaatttcta aagcaaatta tcaacgtccc caaaagctta aaaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.00150.a.B3.GE40743
| HARVESTED ON: | 11/21/2016 |
| SEQUENCED ON: | 11/22/2016 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS K
Validated NT Sequence
ctttagaaat ttgttgagca cgctcaaagg agagactttg ttcagccaca tctttcgtaa tcacagaacc cgcaccgact gttgctccat taccaatagt cacaggcgca accagcgagc tattagaacc gataaaaact gcatcgccaa tagtggtttt gtgtttattt gcaccatcat aattgcaggt gattgtacct gccccaatat ttgattcagc accgatctcg gcatcaccta aataggtgaa atggttagct ttactgccta gaccgatcgt tgtatttttg acttcaacaa agttgccgat atgtacttcg tttgccaatt tagcacctgg acgtaagcgt gcgaatggac caatttgggt attttcccca acaactgctc catcaaaaac gctgtacgct tggactttag tgcccgctgc aatggttgta ttttttaaga tacagccagc accgatttcg acaaagtcac cgagttcaca gttaccttca ataatgacat tcacatcaat acgaacatca tgaccaactt ttactgtacc acgtaaatca aaacgcgcag gatcagcaaa agttacgcct tgctgcataa gttcttttgc ttgttgcttc tgaaattccc gctcaagtgc tgcaagttgc aggcggtcat ttacaccttc aacttcaaat gcgagctctg gttgaattga agcgatttct agaccatcag caacagccat cgcaacgata tcagttaaat agtattcgcc ttgtgcattt tcatttgaaa gcttaggcaa ccactcatga agttttgcat tgctgacaca gtaaatacct gtgttaattt cttgaatttg acgctgagct tcggttgcgt ctttatgttc aacaatcgcc tggattttac cgtcttggcg gacaatacgg ccataacctg ttgggttatc gacatgtaaa gtaatcatgc caataccggt tttatttgat acttcaatca gttgttcaag cgttgtttga cggactaaag gaacatcacc gtataaaatt aatgaaatgc cgtctttagg aagtaccggt aaagtcatct gtaccgcatg accggtccct aactgttctg cttgttcaac ccactgaata ttttcttgtg cgaatgtctt ttttacgtga tcgccaccat gtccataaat ggtaatgatg ttttctgcta atagttgttt tgctgtttta ataacatgtc ctagtagcgg gcgaccagct aaaggttgta gtactttagg gagctgagaa cgcattcgcg ttcctttgcc tgcagcaaga ataatgacag tagttgacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS KANYQRPQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa ctactgtcat tattcttgct gcaggcaaag gaacgcgaat gcgttctcag ctccctaaag tactacaacc tttagctggt cgcccgctac taggacatgt tattaaaaca gcaaaacaac tattagcaga aaacatcatt accatttatg gacatggtgg cgatcacgta aaaaagacat tcgcacaaga aaatattcag tgggttgaac aagcagaaca gttagggacc ggtcatgcgg tacagatgac tttaccggta cttcctaaag acggcatttc attaatttta tacggtgatg ttcctttagt ccgtcaaaca acgcttgaac aactgattga agtatcaaat aaaaccggta ttggcatgat tactttacat gtcgataacc caacaggtta tggccgtatt gtccgccaag acggtaaaat ccaggcgatt gttgaacata aagacgcaac cgaagctcag cgtcaaattc aagaaattaa cacaggtatt tactgtgtca gcaatgcaaa acttcatgag tggttgccta agctttcaaa tgaaaatgca caaggcgaat actatttaac tgatatcgtt gcgatggctg ttgctgatgg tctagaaatc gcttcaattc aaccagagct cgcatttgaa gttgaaggtg taaatgaccg cctgcaactt gcagcacttg agcgggaatt tcagaagcaa caagcaaaag aacttatgca gcaaggcgta acttttgctg atcctgcgcg ttttgattta cgtggtacag taaaagttgg tcatgatgtt cgtattgatg tgaatgtcat tattgaaggt aactgtgaac tcggtgactt tgtcgaaatc ggtgctggct gtatcttaaa aaatacaacc attgcagcgg gcactaaagt ccaagcgtac agcgtttttg atggagcagt tgttggggaa aatacccaaa ttggtccatt cgcacgctta cgtccaggtg ctaaattggc aaacgaagta catatcggca actttgttga agtcaaaaat acaacgatcg gtctaggcag taaagctaac catttcacct atttaggtga tgccgagatc ggtgctgaat caaatattgg ggcaggtaca atcacctgca attatgatgg tgcaaataaa cacaaaacca ctattggcga tgcagttttt atcggttcta atagctcgct ggttgcgcct gtgactattg gtaatggagc aacagtcggt gcgggttctg tgattacgaa agatgtggct gaacaaagtc tctcctttga gcgtgctcaa caaatttcta aagcaaatta tcaacgtccc caatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.00150.a.B3.PS38180
| PURIFICATION DATe: | 1/18/2017 |
| CONCENTRATION: | 61.53mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS K
Validated NT Sequence
ctttagaaat ttgttgagca cgctcaaagg agagactttg ttcagccaca tctttcgtaa tcacagaacc cgcaccgact gttgctccat taccaatagt cacaggcgca accagcgagc tattagaacc gataaaaact gcatcgccaa tagtggtttt gtgtttattt gcaccatcat aattgcaggt gattgtacct gccccaatat ttgattcagc accgatctcg gcatcaccta aataggtgaa atggttagct ttactgccta gaccgatcgt tgtatttttg acttcaacaa agttgccgat atgtacttcg tttgccaatt tagcacctgg acgtaagcgt gcgaatggac caatttgggt attttcccca acaactgctc catcaaaaac gctgtacgct tggactttag tgcccgctgc aatggttgta ttttttaaga tacagccagc accgatttcg acaaagtcac cgagttcaca gttaccttca ataatgacat tcacatcaat acgaacatca tgaccaactt ttactgtacc acgtaaatca aaacgcgcag gatcagcaaa agttacgcct tgctgcataa gttcttttgc ttgttgcttc tgaaattccc gctcaagtgc tgcaagttgc aggcggtcat ttacaccttc aacttcaaat gcgagctctg gttgaattga agcgatttct agaccatcag caacagccat cgcaacgata tcagttaaat agtattcgcc ttgtgcattt tcatttgaaa gcttaggcaa ccactcatga agttttgcat tgctgacaca gtaaatacct gtgttaattt cttgaatttg acgctgagct tcggttgcgt ctttatgttc aacaatcgcc tggattttac cgtcttggcg gacaatacgg ccataacctg ttgggttatc gacatgtaaa gtaatcatgc caataccggt tttatttgat acttcaatca gttgttcaag cgttgtttga cggactaaag gaacatcacc gtataaaatt aatgaaatgc cgtctttagg aagtaccggt aaagtcatct gtaccgcatg accggtccct aactgttctg cttgttcaac ccactgaata ttttcttgtg cgaatgtctt ttttacgtga tcgccaccat gtccataaat ggtaatgatg ttttctgcta atagttgttt tgctgtttta ataacatgtc ctagtagcgg gcgaccagct aaaggttgta gtactttagg gagctgagaa cgcattcgcg ttcctttgcc tgcagcaaga ataatgacag tagttgacat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS KANYQRPQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA CTACTGTCAT TATTCTTGCT GCAGGCAAAG GAACGCGAAT GCGTTCTCAG CTCCCTAAAG TACTACAACC TTTAGCTGGT CGCCCGCTAC TAGGACATGT TATTAAAACA GCAAAACAAC TATTAGCAGA AAACATCATT ACCATTTATG GACATGGTGG CGATCACGTA AAAAAGACAT TCGCACAAGA AAATATTCAG TGGGTTGAAC AAGCAGAACA GTTAGGGACC GGTCATGCGG TACAGATGAC TTTACCGGTA CTTCCTAAAG ACGGCATTTC ATTAATTTTA TACGGTGATG TTCCTTTAGT CCGTCAAACA ACGCTTGAAC AACTGATTGA AGTATCAAAT AAAACCGGTA TTGGCATGAT TACTTTACAT GTCGATAACC CAACAGGTTA TGGCCGTATT GTCCGCCAAG ACGGTAAAAT CCAGGCGATT GTTGAACATA AAGACGCAAC CGAAGCTCAG CGTCAAATTC AAGAAATTAA CACAGGTATT TACTGTGTCA GCAATGCAAA ACTTCATGAG TGGTTGCCTA AGCTTTCAAA TGAAAATGCA CAAGGCGAAT ACTATTTAAC TGATATCGTT GCGATGGCTG TTGCTGATGG TCTAGAAATC GCTTCAATTC AACCAGAGCT CGCATTTGAA GTTGAAGGTG TAAATGACCG CCTGCAACTT GCAGCACTTG AGCGGGAATT TCAGAAGCAA CAAGCAAAAG AACTTATGCA GCAAGGCGTA ACTTTTGCTG ATCCTGCGCG TTTTGATTTA CGTGGTACAG TAAAAGTTGG TCATGATGTT CGTATTGATG TGAATGTCAT TATTGAAGGT AACTGTGAAC TCGGTGACTT TGTCGAAATC GGTGCTGGCT GTATCTTAAA AAATACAACC ATTGCAGCGG GCACTAAAGT CCAAGCGTAC AGCGTTTTTG ATGGAGCAGT TGTTGGGGAA AATACCCAAA TTGGTCCATT CGCACGCTTA CGTCCAGGTG CTAAATTGGC AAACGAAGTA CATATCGGCA ACTTTGTTGA AGTCAAAAAT ACAACGATCG GTCTAGGCAG TAAAGCTAAC CATTTCACCT ATTTAGGTGA TGCCGAGATC GGTGCTGAAT CAAATATTGG GGCAGGTACA ATCACCTGCA ATTATGATGG TGCAAATAAA CACAAAACCA CTATTGGCGA TGCAGTTTTT ATCGGTTCTA ATAGCTCGCT GGTTGCGCCT GTGACTATTG GTAATGGAGC AACAGTCGGT GCGGGTTCTG TGATTACGAA AGATGTGGCT GAACAAAGTC TCTCCTTTGA GCGTGCTCAA CAAATTTCTA AAGCAAATTA TCAACGTCCC CAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for AcbaC.00150.a.B1.PW37634
| PURIFICATION DATe: | 1/20/2015 |
| CONCENTRATION: | 29mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS KANY
Validated NT Sequence
gataatttgc tttagaaatt tgttgagcac gctcaaagga gagactttgt tcagccacat ctttcgtaat cacagaaccc gcaccgactg ttgctccatt accaatagtc acaggcgcaa ccagcgagct attagaaccg ataaaaactg catcgccaat agtggttttg tgtttatttg caccatcata attgcaggtg attgtacctg ccccaatatt tgattcagca ccgatctcgg catcacctaa ataggtgaaa tggttagctt tactgcctag accgatcgtt gtatttttga cttcaacaaa gttgccgata tgtacttcgt ttgccaattt agcacctgga cgtaagcgtg cgaatggacc aatttgggta ttttccccaa caactgctcc atcaaaaacg ctgtacgctt ggactttagt gcccgctgca atggttgtat tttttaagat acagccagca ccgatttcga caaagtcacc gagttcacag ttaccttcaa taatgacatt cacatcaata cgaacatcat gaccaacttt tactgtacca cgtaaatcaa aacgcgcagg atcagcaaaa gttacgcctt gctgcataag ttcttttgct tgttgcttct gaaattcccg ctcaagtgct gcaagttgca ggcggtcatt tacaccttca acttcaaatg cgagctctgg ttgaattgaa gcgatttcta gaccatcagc aacagccatc gcaacgatat cagttaaata gtattcgcct tgtgcatttt catttgaaag cttaggcaac cactcatgaa gttttgcatt gctgacacag taaatacctg tgttaatttc ttgaatttga cgctgagctt cggttgcgtc tttatgttca acaatcgcct ggattttacc gtcttggcgg acaatacggc cataacctgt tgggttatcg acatgtaaag taatcatgcc aataccggtt ttatttgata cttcaatcag ttgttcaagc gttgtttgac ggactaaagg aacatcaccg tataaaatta atgaaatgcc gtctttagga agtaccggta aagtcatctg taccgcatga ccggtcccta actgttctgc ttgttcaacc cactgaatat tttcttgtgc gaatgtcttt tttacgtgat cgccaccatg tccataaatg gtaatgatgt tttctgctaa tagttgtttt gctgttttaa taacatgtcc tagtagcggg cgaccagcta aaggttgtag tactttaggg agctgagaac gcattcgcgt tcctttgcct gcagcaagaa taatgacagt agttgacata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS TTVIILAAGK GTRMRSQLPK VLQPLAGRPL LGHVIKTAKQ LLAENIITIY GHGGDHVKKT FAQENIQWVE QAEQLGTGHA VQMTLPVLPK DGISLILYGD VPLVRQTTLE QLIEVSNKTG IGMITLHVDN PTGYGRIVRQ DGKIQAIVEH KDATEAQRQI QEINTGIYCV SNAKLHEWLP KLSNENAQGE YYLTDIVAMA VADGLEIASI QPELAFEVEG VNDRLQLAAL EREFQKQQAK ELMQQGVTFA DPARFDLRGT VKVGHDVRID VNVIIEGNCE LGDFVEIGAG CILKNTTIAA GTKVQAYSVF DGAVVGENTQ IGPFARLRPG AKLANEVHIG NFVEVKNTTI GLGSKANHFT YLGDAEIGAE SNIGAGTITC NYDGANKHKT TIGDAVFIGS NSSLVAPVTI GNGATVGAGS VITKDVAEQS LSFERAQQIS KANYQRPQKL KK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA CTACTGTCAT TATTCTTGCT GCAGGCAAAG GAACGCGAAT GCGTTCTCAG CTCCCTAAAG TACTACAACC TTTAGCTGGT CGCCCGCTAC TAGGACATGT TATTAAAACA GCAAAACAAC TATTAGCAGA AAACATCATT ACCATTTATG GACATGGTGG CGATCACGTA AAAAAGACAT TCGCACAAGA AAATATTCAG TGGGTTGAAC AAGCAGAACA GTTAGGGACC GGTCATGCGG TACAGATGAC TTTACCGGTA CTTCCTAAAG ACGGCATTTC ATTAATTTTA TACGGTGATG TTCCTTTAGT CCGTCAAACA ACGCTTGAAC AACTGATTGA AGTATCAAAT AAAACCGGTA TTGGCATGAT TACTTTACAT GTCGATAACC CAACAGGTTA TGGCCGTATT GTCCGCCAAG ACGGTAAAAT CCAGGCGATT GTTGAACATA AAGACGCAAC CGAAGCTCAG CGTCAAATTC AAGAAATTAA CACAGGTATT TACTGTGTCA GCAATGCAAA ACTTCATGAG TGGTTGCCTA AGCTTTCAAA TGAAAATGCA CAAGGCGAAT ACTATTTAAC TGATATCGTT GCGATGGCTG TTGCTGATGG TCTAGAAATC GCTTCAATTC AACCAGAGCT CGCATTTGAA GTTGAAGGTG TAAATGACCG CCTGCAACTT GCAGCACTTG AGCGGGAATT TCAGAAGCAA CAAGCAAAAG AACTTATGCA GCAAGGCGTA ACTTTTGCTG ATCCTGCGCG TTTTGATTTA CGTGGTACAG TAAAAGTTGG TCATGATGTT CGTATTGATG TGAATGTCAT TATTGAAGGT AACTGTGAAC TCGGTGACTT TGTCGAAATC GGTGCTGGCT GTATCTTAAA AAATACAACC ATTGCAGCGG GCACTAAAGT CCAAGCGTAC AGCGTTTTTG ATGGAGCAGT TGTTGGGGAA AATACCCAAA TTGGTCCATT CGCACGCTTA CGTCCAGGTG CTAAATTGGC AAACGAAGTA CATATCGGCA ACTTTGTTGA AGTCAAAAAT ACAACGATCG GTCTAGGCAG TAAAGCTAAC CATTTCACCT ATTTAGGTGA TGCCGAGATC GGTGCTGAAT CAAATATTGG GGCAGGTACA ATCACCTGCA ATTATGATGG TGCAAATAAA CACAAAACCA CTATTGGCGA TGCAGTTTTT ATCGGTTCTA ATAGCTCGCT GGTTGCGCCT GTGACTATTG GTAATGGAGC AACAGTCGGT GCGGGTTCTG TGATTACGAA AGATGTGGCT GAACAAAGTC TCTCCTTTGA GCGTGCTCAA CAAATTTCTA AAGCAAATTA TCAACGTCCC CAAAAGCTTA AAAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT