SSGCID: Target Page

AcbaC.00146.a

chorismate mutase

CENTER ID:	AcbaC.00146.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00146.a.B1.GE43933	full length		1	369

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1325
GenBank:	AKA31110.1
PGAT:	acinetobacter:ABUW_1365
RefSeq:	WP_000606364.1
UniProt:	V5VBH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MINDDQNKTT SLSLEQIRED IDSVDQQIQE LLNRRASLAE AVAKAKFASE ENPLFYRPER EAQVLRKVME RNQGPLSDVT MARLFREIMS ACLALEAPQS IAFLGPEGTF TQSAVLKHFG KDAVVRPLPT IDEVFREVEA GSAHYGVVPV ENSSEGIVNH TLDCFKTSNL NVIGEVELRI HHQFLVSENT RKDSIKQIYA HQQTLAQCRQ WLDAHYPGVE RVALNSNAEA ARRIRNEWHS AAIASDIAAG MYNLEILHSN IEDNPENTTR FLVIGREKIP QSGNDKTSLL ISAHDRAGAL LEILAPFAKH NISLTSIETR PALPEKWAYV FFIDLEGHID QENVAAAINE IRPMVKELRI LGSYPAAVL

NT Sequence

ATGATCAACG ATGATCAAAA CAAAACGACT TCTCTTAGTT TAGAACAAAT TCGCGAAGAT ATTGATAGCG TAGATCAACA GATTCAAGAG TTATTAAATC GCCGCGCAAG CCTTGCTGAA GCTGTTGCGA AAGCTAAATT TGCTTCTGAA GAGAATCCTT TGTTTTATCG TCCTGAACGT GAAGCACAAG TTTTACGTAA AGTTATGGAG CGCAATCAAG GGCCTTTATC TGACGTAACA ATGGCCCGTT TGTTCCGTGA AATTATGTCT GCTTGCCTTG CTTTAGAAGC TCCGCAAAGT ATTGCATTTT TAGGCCCAGA AGGTACTTTT ACTCAATCTG CCGTACTTAA ACATTTTGGT AAAGATGCGG TTGTCCGTCC GCTACCAACT ATTGATGAAG TATTCCGTGA AGTGGAAGCG GGTAGTGCGC ATTATGGTGT AGTTCCGGTT GAAAACTCAT CTGAAGGTAT CGTAAACCAT ACTTTAGATT GTTTTAAAAC TTCGAATTTA AATGTGATTG GTGAAGTTGA ATTGCGTATT CATCATCAAT TCCTTGTTTC TGAAAATACA CGTAAAGACA GTATTAAGCA GATTTATGCG CATCAGCAAA CTTTGGCTCA ATGTCGTCAG TGGTTAGACG CACATTATCC GGGTGTTGAG CGTGTTGCCT TAAACTCAAA TGCAGAAGCT GCACGCCGTA TTCGCAATGA ATGGCATTCA GCGGCGATTG CGTCCGATAT TGCAGCAGGT ATGTATAACC TTGAAATCTT GCATAGTAAT ATTGAAGATA ATCCAGAAAA TACCACTCGC TTCTTAGTGA TTGGCCGTGA AAAAATTCCA CAAAGTGGCA ATGACAAAAC TTCATTATTA ATTTCAGCGC ATGACCGTGC AGGCGCTTTA TTAGAAATTT TAGCGCCGTT TGCAAAACAT AATATTAGCT TAACGAGCAT TGAGACCCGT CCAGCATTAC CTGAAAAATG GGCATATGTA TTCTTTATTG ACTTAGAAGG TCATATCGAT CAGGAAAATG TGGCTGCGGC AATCAATGAG ATTCGTCCAA TGGTAAAAGA ACTTCGTATT TTAGGTTCTT ATCCTGCTGC TGTTCTTTAA

Details for AcbaC.00146.a.B1.GE43933

HARVESTED ON:	12/3/2020
SEQUENCED ON:	12/16/2020
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI NDDQNKTTSL SLEQIREDID SVDQQIQELL NRRASLAEAV AKAKFASEEN PLFYRPEREA QVLRKVMERN QGPLSDVTMA RLFREIMSAC LALEAPQSIA FLGPEGTFTQ SAVLKHFGKD AVVRPLPTID EVFREVEAGS AHYGVVPVEN SSEGIVNHTL DCFKTSNLNV IGEVELRIHH QFLVSENTRK DSIKQIYAHQ QTLAQCRQWL DAHYPGVERV ALNSNAEAAR RIRNEWHSAA IASDIAAGMY NLEILHSNIE DNPENTTRFL VIGREKIPQS GNDKTSLLIS AHDRAGALLE ILAPFAKHNI SLTSIETRPA LPEKWAYVFF IDLEGHIDQE NVAAAINEIR PMVKELRILG SYPAAVL

Validated NT Sequence

atcttactca cttaaagaac agcagcagga taagaaccta aaatacgaag ttcttttacc attggacgaa tctcattgat tgccgcagcc acattttcct gatcgatatg accttctaag tcaataaaga atacatatgc ccatttttca ggtaatgctg gacgggtctc aatgctcgtt aagctaatat tatgttttgc aaacggcgct aaaatttcta ataaagcgcc tgcacggtca tgcgctgaaa ttaataatga agttttgtca ttgccacttt gtggaatttt ttcacggcca atcactaaga agcgagtggt attttctgga ttatcttcaa tattactatg caagatttca aggttataca tacctgctgc aatatcggac gcaatcgccg ctgaatgcca ttcattgcga atacggcgtg cagcttctgc atttgagttt aaggcaacac gctcaacacc cggataatgt gcgtctaacc actgacgaca ttgagccaaa gtttgctgat gcgcataaat ctgcttaata ctgtctttac gtgtattttc agaaacaagg aattgatgat gaatacgcaa ttcaacttca ccaatcacat ttaaattcga agttttaaaa caatctaaag tatggtttac gataccttca gatgagtttt caaccggaac tacaccataa tgcgcactac ccgcttccac ttcacggaat acttcatcaa tagttggtag cggacggaca accgcatctt taccaaaatg tttaagtacg gcagattgag taaaagtacc ttctgggcct aaaaatgcaa tactttgcgg agcttctaaa gcaaggcaag cagacataat ttcacggaac aaacgggcca ttgttacgtc agataaaggc ccttgattgc gctccataac tttacgtaaa acttgtgctt cacgttcagg acgataaaac aaaggattct cttcagaagc aaatttagct ttcgcaacag cttcagcaag gcttgcgcgg cgatttaata actcttgaat ctgttgatct acgctatcaa tatcttcgcg aatttgttct aaactaagag aagtcgtttt gttttgatca tcgttgatca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca acgatgatca aaacaaaacg acttctctta gtttagaaca aattcgcgaa gatattgata gcgtagatca acagattcaa gagttattaa atcgccgcgc aagccttgct gaagctgttg cgaaagctaa atttgcttct gaagagaatc ctttgtttta tcgtcctgaa cgtgaagcac aagttttacg taaagttatg gagcgcaatc aagggccttt atctgacgta acaatggccc gtttgttccg tgaaattatg tctgcttgcc ttgctttaga agctccgcaa agtattgcat ttttaggccc agaaggtact tttactcaat ctgccgtact taaacatttt ggtaaagatg cggttgtccg tccgctacca actattgatg aagtattccg tgaagtggaa gcgggtagtg cgcattatgg tgtagttccg gttgaaaact catctgaagg tatcgtaaac catactttag attgttttaa aacttcgaat ttaaatgtga ttggtgaagt tgaattgcgt attcatcatc aattccttgt ttctgaaaat acacgtaaag acagtattaa gcagatttat gcgcatcagc aaactttggc tcaatgtcgt cagtggttag acgcacatta tccgggtgtt gagcgtgttg ccttaaactc aaatgcagaa gctgcacgcc gtattcgcaa tgaatggcat tcagcggcga ttgcgtccga tattgcagca ggtatgtata accttgaaat cttgcatagt aatattgaag ataatccaga aaataccact cgcttcttag tgattggccg tgaaaaaatt ccacaaagtg gcaatgacaa aacttcatta ttaatttcag cgcatgaccg tgcaggcgct ttattagaaa ttttagcgcc gtttgcaaaa cataatatta gcttaacgag cattgagacc cgtccagcat tacctgaaaa atgggcatat gtattcttta ttgacttaga aggtcatatc gatcaggaaa atgtggctgc ggcaatcaat gagattcgtc caatggtaaa agaacttcgt attttaggtt cttatcctgc tgctgttctt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat