AcbaC.00145.a

aspartyl-tRNA synthetase

CENTER ID: AcbaC.00145.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00145.a.B2.GE40736 truncation 1 2 270
AcbaC.00145.a.B3.GE40737 truncation 2 2 505
AcbaC.00145.a.B4.GE40738 truncatin 3 2 530
AcbaC.00145.a.B6.GE40740 truncation 5 2 568
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
GenBank: AKA30368.1
PATRIC ID: fig|470.1345.peg.568
PGAT: acinetobacter:ABUW_0598
RefSeq: WP_000986451.1
UniProt: N0A5J2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMRTHYCGSL TEAQIDQTVT LCGWVHRRRD HGGVIFLDMR DRDGLVQVVI DPDTPEAFAT ADKARSEYVL KITGRVRRRY EGTENPNMVS GQIEVLGKEI EVLAASETPP FPLNDDTINV SEEHRLKYRF LDIRRPEMLE RLRFRSKVTN LIRNYLDDHG FLDVETPILT RATPEGARDY LVPSRVQNGS FYALPQSPQL FKQLLMVGGI DRYYQIAKCF RDEDLRADRQ PEFTQIDIET SFLNDDDIMD LMEGMTVKLF NDLLGVKFEK FRRMPYSEAM RDYASDKPDL RIPLKLVDVA DLMQEVEFKV FAGPAKDPKG RIAALRVPGA GSLTRSQIDE YTKFVGIYGA KGLAYIKVNE IEKGIEGLQS PIVKFIEPIV MQLLERVGAE NGDIVFFGAD KAKIVNDAMG ALRVKIGHDL NLATCEWAPL WVVDFPMFEE TDDGKWTSVH HPFTLPKSSV EDVKSNPGEA LSVAYDMVLN GTEVGGGSLR IYTLEMQKAI FEALGISDEE AEEKFSFLLN ALRYGAPPHG GLAFGLDRLV MLMTGATSIR DVIAFPKTKT AECPLTQAPA PVEANQLRDL GIRLREQQKK EA
NT Sequence
ATGATGCGAA CTCATTACTG TGGCTCTTTA ACTGAAGCCC AAATCGACCA GACCGTTACA TTATGCGGTT GGGTACATCG TCGCCGTGAC CATGGCGGTG TTATTTTCCT TGATATGCGT GACCGTGATG GTCTCGTTCA AGTGGTTATT GACCCAGATA CTCCAGAAGC ATTTGCAACT GCAGATAAAG CACGTTCTGA ATATGTATTA AAAATTACAG GTCGTGTTCG TCGCCGTTAT GAAGGTACTG AAAACCCAAA TATGGTGAGT GGTCAAATCG AAGTTTTAGG CAAAGAAATC GAAGTTCTTG CTGCATCTGA AACTCCACCA TTCCCATTAA ATGACGACAC TATTAACGTG TCAGAAGAAC ACCGTTTAAA ATATCGTTTC TTGGACATCC GTCGTCCAGA AATGTTAGAG CGTTTACGCT TCCGTTCTAA AGTAACCAAC TTGATTCGTA ACTATTTAGA CGATCATGGT TTCTTAGACG TTGAAACTCC GATTTTAACT CGTGCAACCC CTGAAGGTGC GCGTGACTAT TTAGTACCAA GCCGTGTACA AAATGGTAGC TTCTATGCAC TTCCACAATC ACCACAATTG TTCAAACAAT TATTGATGGT TGGTGGTATT GATCGCTACT ACCAAATCGC GAAATGTTTC CGTGATGAAG ACTTACGTGC TGACCGTCAG CCTGAATTCA CTCAAATCGA CATTGAAACA TCGTTCTTAA ACGACGATGA CATTATGGAT TTGATGGAAG GCATGACTGT TAAATTATTT AACGACTTGC TTGGCGTAAA ATTTGAAAAA TTCCGCCGTA TGCCATACAG CGAAGCAATG CGTGATTATG CATCTGACAA ACCAGACTTG CGTATTCCGT TAAAACTCGT TGATGTTGCA GACTTAATGC AAGAAGTTGA GTTCAAAGTA TTTGCAGGTC CAGCTAAAGA CCCTAAAGGC CGTATTGCAG CACTTCGTGT ACCGGGCGCA GGTTCATTAA CACGTAGTCA AATTGATGAA TACACTAAAT TTGTCGGTAT CTACGGTGCA AAAGGCTTAG CGTACATTAA AGTCAATGAA ATCGAAAAAG GAATTGAAGG TCTTCAATCT CCTATCGTTA AATTCATCGA ACCAATCGTA ATGCAATTGC TTGAACGTGT TGGCGCTGAA AATGGCGATA TCGTATTCTT TGGTGCGGAC AAAGCTAAAA TTGTAAATGA TGCAATGGGT GCACTTCGTG TCAAGATCGG TCATGACTTG AACCTTGCGA CTTGTGAGTG GGCTCCACTT TGGGTTGTTG ACTTCCCAAT GTTTGAAGAA ACTGACGATG GCAAATGGAC TTCTGTACAC CATCCATTCA CATTACCTAA ATCTAGCGTA GAAGACGTGA AGAGCAACCC AGGCGAAGCA CTTTCTGTTG CTTACGACAT GGTATTGAAT GGTACTGAAG TTGGTGGTGG TTCACTTCGT ATTTACACAT TAGAAATGCA AAAAGCGATT TTTGAAGCGC TTGGTATTTC CGATGAAGAA GCAGAAGAAA AATTCAGCTT CTTACTCAAT GCGCTTCGTT ACGGCGCACC TCCTCACGGT GGTTTGGCAT TTGGTCTTGA CCGTTTAGTA ATGCTTATGA CTGGCGCGAC TTCAATTCGT GATGTTATTG CGTTCCCTAA AACGAAAACT GCTGAATGTC CACTTACTCA AGCACCGGCT CCTGTGGAAG CAAACCAGCT TCGTGACTTG GGCATCCGCT TACGTGAACA ACAAAAGAAA GAAGCTTAA
Details for AcbaC.00145.a.B2.GE40736
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR THYCGSLTEA QIDQTVTLCG WVHRRRDHGG VIFLDMRDRD GLVQVVIDPD TPEAFATADK ARSEYVLKIT GRVRRRYEGT ENPNMVSGQI EVLGKEIEVL AASETPPFPL NDDTINVSEE HRLKYRFLDI RRPEMLERLR FRSKVTNLIR NYLDDHGFLD VETPILTRAT PEGARDYLVP SRVQNGSFYA LPQSPQLFKQ LLMVGGIDRY YQIAKCFRDE DLRADRQPEF TQIDIETSFL NDDDIMDLME GMTVKLFNDL LGVKFEK
Validated NT Sequence
atcttactca cttatttttc aaattttacg ccaagcaagt cgttaaataa tttaacagtc atgccttcca tcaaatccat aatgtcatcg tcgtttaaga acgatgtttc aatgtcgatt tgagtgaatt caggctgacg gtcagcacgt aagtcttcat cacggaaaca tttcgcgatt tggtagtagc gatcaatacc accaaccatc aataattgtt tgaacaattg tggtgattgt ggaagtgcat agaagctacc attttgtaca cggcttggta ctaaatagtc acgcgcacct tcaggggttg cacgagttaa aatcggagtt tcaacgtcta agaaaccatg atcgtctaaa tagttacgaa tcaagttggt tactttagaa cggaagcgta aacgctctaa catttctgga cgacggatgt ccaagaaacg atattttaaa cggtgttctt ctgacacgtt aatagtgtcg tcatttaatg ggaatggtgg agtttcagat gcagcaagaa cttcgatttc tttgcctaaa acttcgattt gaccactcac catatttggg ttttcagtac cttcataacg gcgacgaaca cgacctgtaa tttttaatac atattcagaa cgtgctttat ctgcagttgc aaatgcttct ggagtatctg ggtcaataac cacttgaacg agaccatcac ggtcacgcat atcaaggaaa ataacaccgc catggtcacg gcgacgatgt acccaaccgc ataatgtaac ggtctggtcg atttgggctt cagttaaaga gccacagtaa tgagttcgca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMR THYCGSLTEA QIDQTVTLCG WVHRRRDHGG VIFLDMRDRD GLVQVVIDPD TPEAFATADK ARSEYVLKIT GRVRRRYEGT ENPNMVSGQI EVLGKEIEVL AASETPPFPL NDDTINVSEE HRLKYRFLDI RRPEMLERLR FRSKVTNLIR NYLDDHGFLD VETPILTRAT PEGARDYLVP SRVQNGSFYA LPQSPQLFKQ LLMVGGIDRY YQIAKCFRDE DLRADRQPEF TQIDIETSFL NDDDIMDLME GMTVKLFNDL LGVKFEK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa ctcattactg tggctcttta actgaagccc aaatcgacca gaccgttaca ttatgcggtt gggtacatcg tcgccgtgac catggcggtg ttattttcct tgatatgcgt gaccgtgatg gtctcgttca agtggttatt gacccagata ctccagaagc atttgcaact gcagataaag cacgttctga atatgtatta aaaattacag gtcgtgttcg tcgccgttat gaaggtactg aaaacccaaa tatggtgagt ggtcaaatcg aagttttagg caaagaaatc gaagttcttg ctgcatctga aactccacca ttcccattaa atgacgacac tattaacgtg tcagaagaac accgtttaaa atatcgtttc ttggacatcc gtcgtccaga aatgttagag cgtttacgct tccgttctaa agtaaccaac ttgattcgta actatttaga cgatcatggt ttcttagacg ttgaaactcc gattttaact cgtgcaaccc ctgaaggtgc gcgtgactat ttagtaccaa gccgtgtaca aaatggtagc ttctatgcac ttccacaatc accacaattg ttcaaacaat tattgatggt tggtggtatt gatcgctact accaaatcgc gaaatgtttc cgtgatgaag acttacgtgc tgaccgtcag cctgaattca ctcaaatcga cattgaaaca tcgttcttaa acgacgatga cattatggat ttgatggaag gcatgactgt taaattattt aacgacttgc ttggcgtaaa atttgaaaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for AcbaC.00145.a.B3.GE40737
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR THYCGSLTEA QIDQTVTLCG WVHRRRDHGG VIFLDMRDRD GLVQVVIDPD TPEAFATADK ARSEYVLKIT GRVRRRYEGT ENPNMVSGQI EVLGKEIEVL AASETPPFPL NDDTINVSEE HRLKYRFLDI RRPEMLERLR FRSKVTNLIR NYLDDHGFLD VETPILTRAT PEGARDYLVP SRVQNGSFYA LPQSPQLFKQ LLMVGGIDRY YQIAKCFRDE DLRADRQPEF TQIDIETSFL NDDDIMDLME GMTVKLFNDL LGVKFEKFRR MPYSEAMRDY ASDKPDLRIP LKLVDVADLM QEVEFKVFAG PAKDPKGRIA ALRVPGAGSL TRSQIDEYTK FVGIYGAKGL AYIKVNEIEK GIEGLQSPIV KFIEPIVMQL LERVGAENGD IVFFGADKAK IVNDAMGALR VKIGHDLNLA TCEWAPLWVV DFPMFEETDD GKWTSVHHPF TLPKSSVEDV KSNPGEALSV AYDMVLNGTE VGGGSLRIYT LEMQKAIFEA LG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa ctcattactg tggctcttta actgaagccc aaatcgacca gaccgttaca ttatgcggtt gggtacatcg tcgccgtgac catggcggtg ttattttcct tgatatgcgt gaccgtgatg gtctcgttca agtggttatt gacccagata ctccagaagc atttgcaact gcagataaag cacgttctga atatgtatta aaaattacag gtcgtgttcg tcgccgttat gaaggtactg aaaacccaaa tatggtgagt ggtcaaatcg aagttttagg caaagaaatc gaagttcttg ctgcatctga aactccacca ttcccattaa atgacgacac tattaacgtg tcagaagaac accgtttaaa atatcgtttc ttggacatcc gtcgtccaga aatgttagag cgtttacgct tccgttctaa agtaaccaac ttgattcgta actatttaga cgatcatggt ttcttagacg ttgaaactcc gattttaact cgtgcaaccc ctgaaggtgc gcgtgactat ttagtaccaa gccgtgtaca aaatggtagc ttctatgcac ttccacaatc accacaattg ttcaaacaat tattgatggt tggtggtatt gatcgctact accaaatcgc gaaatgtttc cgtgatgaag acttacgtgc tgaccgtcag cctgaattca ctcaaatcga cattgaaaca tcgttcttaa acgacgatga cattatggat ttgatggaag gcatgactgt taaattattt aacgacttgc ttggcgtaaa atttgaaaaa ttccgccgta tgccatacag cgaagcaatg cgtgattatg catctgacaa accagacttg cgtattccgt taaaactcgt tgatgttgca gacttaatgc aagaagttga gttcaaagta tttgcaggtc cagctaaaga ccctaaaggc cgtattgcag cacttcgtgt accgggcgca ggttcattaa cacgtagtca aattgatgaa tacactaaat ttgtcggtat ctacggtgca aaaggcttag cgtacattaa agtcaatgaa atcgaaaaag gaattgaagg tcttcaatct cctatcgtta aattcatcga accaatcgta atgcaattgc ttgaacgtgt tggcgctgaa aatggcgata tcgtattctt tggtgcggac aaagctaaaa ttgtaaatga tgcaatgggt gcacttcgtg tcaagatcgg tcatgacttg aaccttgcga cttgtgagtg ggctccactt tgggttgttg acttcccaat gtttgaagaa actgacgatg gcaaatggac ttctgtacac catccattca cattacctaa atctagcgta gaagacgtga agagcaaccc aggcgaagca ctttctgttg cttacgacat ggtattgaat ggtactgaag ttggtggtgg ttcacttcgt atttacacat tagaaatgca aaaagcgatt tttgaagcgc ttggttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.00145.a.B4.GE40738
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 61kDa
OBSERVED MW: 63kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR THYCGSLTEA QIDQTVTLCG WVHRRRDHGG VIFLDMRDRD GLVQVVIDPD TPEAFATADK ARSEYVLKIT GRVRRRYEGT ENPNMVSGQI EVLGKEIEVL AASETPPFPL NDDTINVSEE HRLKYRFLDI RRPEMLERLR FRSKVTNLIR NYLDDHGFLD VETPILTRAT PEGARDYLVP SRVQNGSFYA LPQSPQLFKQ LLMVGGIDRY YQIAKCFRDE DLRADRQPEF TQIDIETSFL NDDDIMDLME GMTVKLFNDL LGVKFEKFRR MPYSEAMRDY ASDKPDLRIP LKLVDVADLM QEVEFKVFAG PAKDPKGRIA ALRVPGAGSL TRSQIDEYTK FVGIYGAKGL AYIKVNEIEK GIEGLQSPIV KFIEPIVMQL LERVGAENGD IVFFGADKAK IVNDAMGALR VKIGHDLNLA TCEWAPLWVV DFPMFEETDD GKWTSVHHPF TLPKSSVEDV KSNPGEALSV AYDMVLNGTE VGGGSLRIYT LEMQKAIFEA LGISDEEAEE KFSFLLNALR YGAPPHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa ctcattactg tggctcttta actgaagccc aaatcgacca gaccgttaca ttatgcggtt gggtacatcg tcgccgtgac catggcggtg ttattttcct tgatatgcgt gaccgtgatg gtctcgttca agtggttatt gacccagata ctccagaagc atttgcaact gcagataaag cacgttctga atatgtatta aaaattacag gtcgtgttcg tcgccgttat gaaggtactg aaaacccaaa tatggtgagt ggtcaaatcg aagttttagg caaagaaatc gaagttcttg ctgcatctga aactccacca ttcccattaa atgacgacac tattaacgtg tcagaagaac accgtttaaa atatcgtttc ttggacatcc gtcgtccaga aatgttagag cgtttacgct tccgttctaa agtaaccaac ttgattcgta actatttaga cgatcatggt ttcttagacg ttgaaactcc gattttaact cgtgcaaccc ctgaaggtgc gcgtgactat ttagtaccaa gccgtgtaca aaatggtagc ttctatgcac ttccacaatc accacaattg ttcaaacaat tattgatggt tggtggtatt gatcgctact accaaatcgc gaaatgtttc cgtgatgaag acttacgtgc tgaccgtcag cctgaattca ctcaaatcga cattgaaaca tcgttcttaa acgacgatga cattatggat ttgatggaag gcatgactgt taaattattt aacgacttgc ttggcgtaaa atttgaaaaa ttccgccgta tgccatacag cgaagcaatg cgtgattatg catctgacaa accagacttg cgtattccgt taaaactcgt tgatgttgca gacttaatgc aagaagttga gttcaaagta tttgcaggtc cagctaaaga ccctaaaggc cgtattgcag cacttcgtgt accgggcgca ggttcattaa cacgtagtca aattgatgaa tacactaaat ttgtcggtat ctacggtgca aaaggcttag cgtacattaa agtcaatgaa atcgaaaaag gaattgaagg tcttcaatct cctatcgtta aattcatcga accaatcgta atgcaattgc ttgaacgtgt tggcgctgaa aatggcgata tcgtattctt tggtgcggac aaagctaaaa ttgtaaatga tgcaatgggt gcacttcgtg tcaagatcgg tcatgacttg aaccttgcga cttgtgagtg ggctccactt tgggttgttg acttcccaat gtttgaagaa actgacgatg gcaaatggac ttctgtacac catccattca cattacctaa atctagcgta gaagacgtga agagcaaccc aggcgaagca ctttctgttg cttacgacat ggtattgaat ggtactgaag ttggtggtgg ttcacttcgt atttacacat tagaaatgca aaaagcgatt tttgaagcgc ttggtatttc cgatgaagaa gcagaagaaa aattcagctt cttactcaat gcgcttcgtt acggcgcacc tcctcacggt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for AcbaC.00145.a.B6.GE40740
HARVESTED ON: 11/21/2016
SEQUENCED ON: 11/22/2016
EXPECTED MW: 65kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR THYCGSLTEA QIDQTVTLCG WVHRRRDHGG VIFLDMRDRD GLVQVVIDPD TPEAFATADK ARSEYVLKIT GRVRRRYEGT ENPNMVSGQI EVLGKEIEVL AASETPPFPL NDDTINVSEE HRLKYRFLDI RRPEMLERLR FRSKVTNLIR NYLDDHGFLD VETPILTRAT PEGARDYLVP SRVQNGSFYA LPQSPQLFKQ LLMVGGIDRY YQIAKCFRDE DLRADRQPEF TQIDIETSFL NDDDIMDLME GMTVKLFNDL LGVKFEKFRR MPYSEAMRDY ASDKPDLRIP LKLVDVADLM QEVEFKVFAG PAKDPKGRIA ALRVPGAGSL TRSQIDEYTK FVGIYGAKGL AYIKVNEIEK GIEGLQSPIV KFIEPIVMQL LERVGAENGD IVFFGADKAK IVNDAMGALR VKIGHDLNLA TCEWAPLWVV DFPMFEETDD GKWTSVHHPF TLPKSSVEDV KSNPGEALSV AYDMVLNGTE VGGGSLRIYT LEMQKAIFEA LGISDEEAEE KFSFLLNALR YGAPPHGGLA FGLDRLVMLM TGATSIRDVI AFPKTKTAEC PLTQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa ctcattactg tggctcttta actgaagccc aaatcgacca gaccgttaca ttatgcggtt gggtacatcg tcgccgtgac catggcggtg ttattttcct tgatatgcgt gaccgtgatg gtctcgttca agtggttatt gacccagata ctccagaagc atttgcaact gcagataaag cacgttctga atatgtatta aaaattacag gtcgtgttcg tcgccgttat gaaggtactg aaaacccaaa tatggtgagt ggtcaaatcg aagttttagg caaagaaatc gaagttcttg ctgcatctga aactccacca ttcccattaa atgacgacac tattaacgtg tcagaagaac accgtttaaa atatcgtttc ttggacatcc gtcgtccaga aatgttagag cgtttacgct tccgttctaa agtaaccaac ttgattcgta actatttaga cgatcatggt ttcttagacg ttgaaactcc gattttaact cgtgcaaccc ctgaaggtgc gcgtgactat ttagtaccaa gccgtgtaca aaatggtagc ttctatgcac ttccacaatc accacaattg ttcaaacaat tattgatggt tggtggtatt gatcgctact accaaatcgc gaaatgtttc cgtgatgaag acttacgtgc tgaccgtcag cctgaattca ctcaaatcga cattgaaaca tcgttcttaa acgacgatga cattatggat ttgatggaag gcatgactgt taaattattt aacgacttgc ttggcgtaaa atttgaaaaa ttccgccgta tgccatacag cgaagcaatg cgtgattatg catctgacaa accagacttg cgtattccgt taaaactcgt tgatgttgca gacttaatgc aagaagttga gttcaaagta tttgcaggtc cagctaaaga ccctaaaggc cgtattgcag cacttcgtgt accgggcgca ggttcattaa cacgtagtca aattgatgaa tacactaaat ttgtcggtat ctacggtgca aaaggcttag cgtacattaa agtcaatgaa atcgaaaaag gaattgaagg tcttcaatct cctatcgtta aattcatcga accaatcgta atgcaattgc ttgaacgtgt tggcgctgaa aatggcgata tcgtattctt tggtgcggac aaagctaaaa ttgtaaatga tgcaatgggt gcacttcgtg tcaagatcgg tcatgacttg aaccttgcga cttgtgagtg ggctccactt tgggttgttg acttcccaat gtttgaagaa actgacgatg gcaaatggac ttctgtacac catccattca cattacctaa atctagcgta gaagacgtga agagcaaccc aggcgaagca ctttctgttg cttacgacat ggtattgaat ggtactgaag ttggtggtgg ttcacttcgt atttacacat tagaaatgca aaaagcgatt tttgaagcgc ttggtatttc cgatgaagaa gcagaagaaa aattcagctt cttactcaat gcgcttcgtt acggcgcacc tcctcacggt ggtttggcat ttggtcttga ccgtttagta atgcttatga ctggcgcgac ttcaattcgt gatgttattg cgttccctaa aacgaaaact gctgaatgtc cacttactca agcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat