AcbaC.00141.a

malonyl CoA-acyl carrier protein transacylase

CENTER ID:	AcbaC.00141.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00141.a.B1.GE38594	full length		1	328

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00141.a.B1.PW37642	full length		1	328

External Resources

RESOURCE	REFERENCE ID
GenBank:	AKA32816.1
PATRIC ID:	fig\|470.1345.peg.3054
PGAT:	acinetobacter:ABUW_3109
RefSeq:	WP_001274814.1
UniProt:	V5VFX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAKRLEQVA LATKTAFVFP GQGSQKVGML AELAEQFSGV GQTFAEASDA LGFDLWHIAQ SGEGLDQTEN TQPVLLTASI ALWRLWLDLG GLAPKYLAGH SLGEYSALVA AGSMSLADAV KLVNLRGKLM QNAVPQGEGA MAAILGLADD KVVELCQSVS ALGQGSVEAA NYNSQGQVVI AGSTASVQQV MALAKEQSAK AIALPVSVPS HCSLMKPAAE KFAEALEQTA IELPTIPVIQ NVNAGIATDV AQLRQALTAQ LYQSVQWTRT MQSLQDQGIQ YIVECGPGTV LSNLAKRLPN IEKAFSIDSK SKMEDALNAV LVAEGKIA

NT Sequence

ATGTCTGCTA AACGTCTTGA ACAAGTAGCT TTAGCCACAA AAACTGCATT TGTGTTTCCA GGTCAAGGTT CACAAAAAGT TGGTATGCTC GCTGAACTCG CAGAACAGTT TAGTGGAGTT GGGCAAACTT TTGCCGAAGC TTCAGACGCG CTAGGATTTG ATCTTTGGCA CATTGCACAA AGTGGTGAAG GTTTGGACCA AACAGAAAAT ACCCAGCCTG TATTATTAAC TGCCAGTATT GCTTTATGGC GTTTATGGTT AGATTTAGGT GGTTTAGCCC CTAAATACCT TGCAGGTCAC TCTTTAGGTG AATACAGCGC TTTAGTTGCA GCAGGTTCAA TGAGTTTGGC GGATGCAGTT AAGTTAGTTA ATTTGCGTGG AAAACTCATG CAAAATGCTG TTCCTCAAGG GGAAGGCGCT ATGGCTGCTA TTCTTGGTTT AGCTGATGAT AAAGTAGTTG AATTGTGCCA GAGCGTATCT GCATTAGGCC AAGGTTCAGT TGAAGCAGCA AACTATAATT CACAAGGTCA AGTTGTGATT GCTGGTAGTA CTGCATCTGT ACAGCAAGTT ATGGCATTGG CAAAAGAACA ATCTGCTAAA GCAATTGCTT TACCGGTTTC TGTTCCATCA CATTGTTCAT TGATGAAGCC GGCAGCAGAA AAATTTGCAG AAGCTTTGGA ACAAACTGCC ATTGAGCTAC CTACCATTCC TGTAATACAA AATGTCAACG CGGGAATTGC TACCGATGTT GCTCAATTAC GTCAGGCTTT AACAGCACAA TTGTACCAGT CTGTACAATG GACACGTACC ATGCAGTCTC TACAAGACCA AGGTATTCAG TACATTGTTG AGTGTGGCCC AGGTACAGTA TTGAGTAATC TCGCAAAGCG TTTACCAAAT ATTGAAAAAG CTTTTTCTAT TGATAGTAAG AGCAAAATGG AAGATGCCCT AAATGCTGTT TTAGTGGCAG AAGGAAAAAT TGCATGA

Details for AcbaC.00141.a.B1.GE38594

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMS AKRLEQVALA TKTAFVFPGQ GSQKVGMLAE LAEQFSGVGQ TFAEASDALG FDLWHIAQSG EGLDQTENTQ PVLLTASIAL WRLWLDLGGL APKYLAGHSL GEYSALVAAG SMSLADAVKL VNLRGKLMQN AVPQGEGAMA AILGLADDKV VELCQSVSAL GQGSVEAANY NSQGQVVIAG STASVQQVMA LAKEQSAKAI ALPVSVPSHC SLMKPAAEKF AEALEQTAIE LPTIPVIQNV NAGIATDVAQ LRQALTAQLY QSVQWTRTMQ SLQDQGIQYI VECGPGTVLS NLAKRLPNIE KAFSIDSKSK MEDALNAVXX XEGKI

Validated NT Sequence

tgcaattttt ccttcnnnnn nnnaaacagc atttagggca tcttccattt tgctcttact atcaatagaa aaagcttttt caatatttgg taaacgcttt gcgagattac tcaatactgt acctgggcca cactcaacaa tgtactgaat accttggtct tgtagagact gcatggtacg tgtccattgt acagactggt acaattgtgc tgttaaagcc tgacgtaatt gagcaacatc ggtagcaatt cccgcgttga cattttgtat tacaggaatg gtaggtagct caatggcagt ttgttccaaa gcttctgcaa atttttctgc tgccggcttc atcaatgaac aatgtgatgg aacagaaacc ggtaaagcaa ttgctttagc agattgttct tttgccaatg ccataacttg ctgtacagat gcagtactac cagcaatcac aacttgacct tgtgaattat agtttgctgc ttcaactgaa ccttggccta atgcagatac gctctggcac aattcaacta ctttatcatc agctaaacca agaatagcag ccatagcgcc ttccccttga ggaacagcat tttgcatgag ttttccacgc aaattaacta acttaactgc atccgccaaa ctcattgaac ctgctgcaac taaagcgctg tattcaccta aagagtgacc tgcaaggtat ttaggggcta aaccacctaa atctaaccat aaacgccata aagcaatact ggcagttaat aatacaggct gggtattttc tgtttggtcc aaaccttcac cactttgtgc aatgtgccaa agatcaaatc ctagcgcgtc tgaagcttcg gcaaaagttt gcccaactcc actaaactgt tctgcgagtt cagcgagcat accaactttt tgtgaacctt gacctggaaa cacaaatgca gtttttgtgg ctaaagctac ttgttcaaga cgtttagcag acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg ctaaacgtct tgaacaagta gctttagcca caaaaactgc atttgtgttt ccaggtcaag gttcacaaaa agttggtatg ctcgctgaac tcgcagaaca gtttagtgga gttgggcaaa cttttgccga agcttcagac gcgctaggat ttgatctttg gcacattgca caaagtggtg aaggtttgga ccaaacagaa aatacccagc ctgtattatt aactgccagt attgctttat ggcgtttatg gttagattta ggtggtttag cccctaaata ccttgcaggt cactctttag gtgaatacag cgctttagtt gcagcaggtt caatgagttt ggcggatgca gttaagttag ttaatttgcg tggaaaactc atgcaaaatg ctgttcctca aggggaaggc gctatggctg ctattcttgg tttagctgat gataaagtag ttgaattgtg ccagagcgta tctgcattag gccaaggttc agttgaagca gcaaactata attcacaagg tcaagttgtg attgctggta gtactgcatc tgtacagcaa gttatggcat tggcaaaaga acaatctgct aaagcaattg ctttaccggt ttctgttcca tcacattgtt cattgatgaa gccggcagca gaaaaatttg cagaagcttt ggaacaaact gccattgagc tacctaccat tcctgtaata caaaatgtca acgcgggaat tgctaccgat gttgctcaat tacgtcaggc tttaacagca caattgtacc agtctgtaca atggacacgt accatgcagt ctctacaaga ccaaggtatt cagtacattg ttgagtgtgg cccaggtaca gtattgagta atctcgcaaa gcgtttacca aatattgaaa aagctttttc tattgatagt aagagcaaaa tggaagatgc cctaaatgct gttttagtgg cagaaggaaa aattgcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for AcbaC.00141.a.B1.PW37642

PURIFICATION DATe:	2/2/2015
CONCENTRATION:	22.5mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	21
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG CTAAACGTCT TGAACAAGTA GCTTTAGCCA CAAAAACTGC ATTTGTGTTT CCAGGTCAAG GTTCACAAAA AGTTGGTATG CTCGCTGAAC TCGCAGAACA GTTTAGTGGA GTTGGGCAAA CTTTTGCCGA AGCTTCAGAC GCGCTAGGAT TTGATCTTTG GCACATTGCA CAAAGTGGTG AAGGTTTGGA CCAAACAGAA AATACCCAGC CTGTATTATT AACTGCCAGT ATTGCTTTAT GGCGTTTATG GTTAGATTTA GGTGGTTTAG CCCCTAAATA CCTTGCAGGT CACTCTTTAG GTGAATACAG CGCTTTAGTT GCAGCAGGTT CAATGAGTTT GGCGGATGCA GTTAAGTTAG TTAATTTGCG TGGAAAACTC ATGCAAAATG CTGTTCCTCA AGGGGAAGGC GCTATGGCTG CTATTCTTGG TTTAGCTGAT GATAAAGTAG TTGAATTGTG CCAGAGCGTA TCTGCATTAG GCCAAGGTTC AGTTGAAGCA GCAAACTATA ATTCACAAGG TCAAGTTGTG ATTGCTGGTA GTACTGCATC TGTACAGCAA GTTATGGCAT TGGCAAAAGA ACAATCTGCT AAAGCAATTG CTTTACCGGT TTCTGTTCCA TCACATTGTT CATTGATGAA GCCGGCAGCA GAAAAATTTG CAGAAGCTTT GGAACAAACT GCCATTGAGC TACCTACCAT TCCTGTAATA CAAAATGTCA ACGCGGGAAT TGCTACCGAT GTTGCTCAAT TACGTCAGGC TTTAACAGCA CAATTGTACC AGTCTGTACA ATGGACACGT ACCATGCAGT CTCTACAAGA CCAAGGTATT CAGTACATTG TTGAGTGTGG CCCAGGTACA GTATTGAGTA ATCTCGCAAA GCGTTTACCA AATATTGAAA AAGCTTTTTC TATTGATAGT AAGAGCAAAA TGGAAGATGC CCTAAATGCT GTTTTAGTGG CAGAAGGAAA AATTGCATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT