AcbaC.00137.c
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanyl ligase (MurF)
| CENTER ID: | AcbaC.00137.c |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00137.c.B1.GE38592 | full length | 1 | 466 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4ZIY
DEPOSITED: 4/28/2015
DETERMINATION: XRay
CLONE: AcbaC.00137.c.B1.GE38592
PROTEIN: AcbaC.00137.c.B1.PS02364
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30071.1 |
| PATRIC ID: | fig|470.1345.peg.272 |
| PGAT: | acinetobacter:ABUW_0285 |
| RefSeq: | WP_000557513.1 |
| UniProt: | A0A0D5YEC3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHTSTTSTVP LEPWTAQQLQ QATQGYWHKD QIPQTEIKRI LTDSRHAESG DAFLALKGER FDAHNFVAQV VANGCQVAIV ERPIDAEIAQ LVVADTRLAL GQLGAYRREQ NAQLKVIALT GSSGKTTTKE MLGSILSRLA PTLITRGNLN NDLGVPMMLL ELRKEHQYAV MELGANHQGE IDYTSKIVQP HVAGILNIGT AHLGEFGGRD GICRAKSEIY RHILPQGVAI VPQQDDFTAE IREAAKSHQI MSFGAGGDVF ATEIELLPQS ANFQLHTPQG SSFVRLPFAG EHNVQNATAA VAFALALGVS LEDIVKGLEQ AQGAKGRLNF IQKTPHLFID DTYNANPTSM RAAAQVLLQQ NGIKVMVMGD IGELGDSSWQ EHHDLGRDLA ELPLDHIVAV GQFASAALEG AGLHSTKLKA FQTQAEALPF LINLIQTHQP QSMSFLFKGS RFTHMETLMA DLMEKL
NT Sequence
ATGCATACTT CAACCACCAG TACCGTGCCT TTGGAACCGT GGACAGCTCA ACAATTACAA CAGGCAACAC AGGGTTACTG GCATAAAGAC CAGATCCCTC AAACTGAAAT TAAACGTATT TTGACTGACT CGCGTCATGC AGAAAGTGGG GATGCCTTTT TAGCACTAAA AGGTGAACGT TTCGACGCGC ATAATTTTGT CGCGCAAGTG GTTGCAAATG GTTGCCAAGT CGCTATTGTC GAGCGGCCTA TAGATGCAGA AATTGCTCAG CTTGTAGTTG CCGATACCCG TTTGGCTTTG GGGCAGCTTG GTGCGTATCG TCGTGAACAA AATGCTCAAT TGAAAGTAAT TGCTTTAACG GGCAGTAGTG GTAAAACCAC CACCAAAGAA ATGCTAGGAA GTATTTTGTC TCGTTTAGCA CCTACATTGA TTACCCGAGG CAATTTAAAT AATGACCTTG GTGTGCCGAT GATGTTGCTT GAGCTTCGAA AGGAGCATCA GTATGCGGTG ATGGAACTTG GTGCAAATCA TCAAGGTGAA ATTGATTACA CATCAAAAAT TGTTCAACCG CATGTTGCTG GCATTTTAAA TATTGGAACT GCTCATTTGG GTGAGTTCGG CGGGCGTGAT GGCATTTGCC GCGCTAAATC AGAAATCTAT CGCCATATTT TACCTCAGGG CGTTGCTATT GTTCCGCAAC AAGATGACTT TACCGCTGAG ATTCGTGAAG CAGCCAAAAG CCATCAAATC ATGAGCTTTG GTGCTGGTGG TGATGTCTTT GCTACAGAGA TTGAATTGTT GCCACAATCT GCCAATTTCC AGTTACATAC GCCTCAAGGT TCAAGTTTTG TAAGACTGCC GTTTGCGGGT GAGCACAATG TGCAAAACGC AACTGCAGCA GTAGCATTTG CTTTGGCGTT AGGTGTTAGC TTGGAAGATA TCGTAAAAGG TCTAGAGCAA GCACAAGGTG CAAAAGGTCG TTTGAATTTT ATTCAAAAAA CGCCTCATTT ATTTATTGAT GATACTTATA ACGCCAATCC AACATCTATG CGTGCTGCGG CACAAGTATT ACTTCAGCAA AATGGCATTA AAGTCATGGT GATGGGGGAT ATTGGGGAGT TAGGTGATAG CAGTTGGCAA GAACATCACG ATTTAGGTCG CGATCTTGCT GAGCTTCCTT TAGACCATAT TGTTGCAGTT GGGCAGTTTG CCTCAGCAGC TCTCGAAGGC GCAGGTCTTC ATTCTACTAA ATTGAAAGCA TTTCAGACTC AAGCTGAGGC GCTTCCATTT TTAATCAATC TAATCCAAAC ACATCAACCC CAGTCCATGA GTTTCCTGTT TAAAGGTTCT CGCTTTACTC ATATGGAAAC GTTGATGGCT GATTTGATGG AGAAACTTTA A
Details for AcbaC.00137.c.B1.GE38592
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMH TSTTSTVPLE PWTAQQLQQA TQGYWHKDQI PQTEIKRILT DSRHAESGDA FLALKGERFD AHNFVAQVVA NGCQVAIVER PIDAEIAQLV VADTRLALGQ LGAYRREQNA QLKVIALTGS SGKTTTKEML GSILSRLAPT LITRGNLNND LGVPMMLLEL RKEHQYAVME LGANHQGEID YTSKIVQPHV AGILNIGTAH LGEFGGRDGI CRAKSEIYRH ILPQGVAIVP QQDDFTAEIR EAAKSHQIMS FGAGGDVFAT EIELLPQSAN FQLHTPQGSS FVRLPFAGEH NVQNATAAVA FALALGVSLE DIVKGLEQAQ GAKGRLNFIQ KTPHLFIDDT YNANPTSMRA AAQVLLQQNG IKVMVMGDIG ELGDSSWQEH HDLGRDLAEL PLDHIVAVGQ FASAALEGAG LHSTKLKAFQ TQAEALPFLI NLIQTHQPQS MSFLFKGSRF THMET
Validated NT Sequence
caacgtttcc atatgagtaa agcgagaacc tttaaacagg aaactcatgg actggggttg atgtgtttgg attagattga ttaaaaatgg aagcgcctca gcttgagtct gaaatgcttt caatttagta gaatgaagac ctgcgccttc gagagctgct gaggcaaact gcccaactgc aacaatatgg tctaaaggaa gctcagcaag atcgcgacct aaatcgtgat gttcttgcca actgctatca cctaactccc caatatcccc catcaccatg actttaatgc cattttgctg aagtaatact tgtgccgcag cacgcataga tgttggattg gcgttataag tatcatcaat aaataaatga ggcgtttttt gaataaaatt caaacgacct tttgcacctt gtgcttgctc tagacctttt acgatatctt ccaagctaac acctaacgcc aaagcaaatg ctactgctgc agttgcgttt tgcacattgt gctcacccgc aaacggcagt cttacaaaac ttgaaccttg aggcgtatgt aactggaaat tggcagattg tggcaacaat tcaatctctg tagcaaagac atcaccacca gcaccaaagc tcatgatttg atggcttttg gctgcttcac gaatctcagc ggtaaagtca tcttgttgcg gaacaatagc aacgccctga ggtaaaatat ggcgatagat ttctgattta gcgcggcaaa tgccatcacg cccgccgaac tcacccaaat gagcagttcc aatatttaaa atgccagcaa catgcggttg aacaattttt gatgtgtaat caatttcacc ttgatgattt gcaccaagtt ccatcaccgc atactgatgc tcctttcgaa gctcaagcaa catcatcggc acaccaaggt cattatttaa attgcctcgg gtaatcaatg taggtgctaa acgagacaaa atacttccta gcatttcttt ggtggtggtt ttaccactac tgcccgttaa agcaattact ttcaattgag cattttgttc acgacgatac gcaccaagct gccccaaagc caaacgggta tcggcaacta caagctgagc aatttctgca tctataggcc gctcgacaat agcgacttgg caaccatttg caaccacttg cgcgacaaaa ttatgcgcgt cgaaacgttc accttttagt gctaaaaagg catccccact ttctgcatga cgcgagtcag tcaaaatacg tttaatttca gtttgaggga tctggtcttt atgccagtaa ccctgtgttg cctgttgtaa ttgttgagct gtccacggtt ccaaaggcac ggtactggtg gttgaagtat gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMH TSTTSTVPLE PWTAQQLQQA TQGYWHKDQI PQTEIKRILT DSRHAESGDA FLALKGERFD AHNFVAQVVA NGCQVAIVER PIDAEIAQLV VADTRLALGQ LGAYRREQNA QLKVIALTGS SGKTTTKEML GSILSRLAPT LITRGNLNND LGVPMMLLEL RKEHQYAVME LGANHQGEID YTSKIVQPHV AGILNIGTAH LGEFGGRDGI CRAKSEIYRH ILPQGVAIVP QQDDFTAEIR EAAKSHQIMS FGAGGDVFAT EIELLPQSAN FQLHTPQGSS FVRLPFAGEH NVQNATAAVA FALALGVSLE DIVKGLEQAQ GAKGRLNFIQ KTPHLFIDDT YNANPTSMRA AAQVLLQQNG IKVMVMGDIG ELGDSSWQEH HDLGRDLAEL PLDHIVAVGQ FASAALEGAG LHSTKLKAFQ TQAEALPFLI NLIQTHQPQS MSFLFKGSRF THMETLMADL MEKL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata cttcaaccac cagtaccgtg cctttggaac cgtggacagc tcaacaatta caacaggcaa cacagggtta ctggcataaa gaccagatcc ctcaaactga aattaaacgt attttgactg actcgcgtca tgcagaaagt ggggatgcct ttttagcact aaaaggtgaa cgtttcgacg cgcataattt tgtcgcgcaa gtggttgcaa atggttgcca agtcgctatt gtcgagcggc ctatagatgc agaaattgct cagcttgtag ttgccgatac ccgtttggct ttggggcagc ttggtgcgta tcgtcgtgaa caaaatgctc aattgaaagt aattgcttta acgggcagta gtggtaaaac caccaccaaa gaaatgctag gaagtatttt gtctcgttta gcacctacat tgattacccg aggcaattta aataatgacc ttggtgtgcc gatgatgttg cttgagcttc gaaaggagca tcagtatgcg gtgatggaac ttggtgcaaa tcatcaaggt gaaattgatt acacatcaaa aattgttcaa ccgcatgttg ctggcatttt aaatattgga actgctcatt tgggtgagtt cggcgggcgt gatggcattt gccgcgctaa atcagaaatc tatcgccata ttttacctca gggcgttgct attgttccgc aacaagatga ctttaccgct gagattcgtg aagcagccaa aagccatcaa atcatgagct ttggtgctgg tggtgatgtc tttgctacag agattgaatt gttgccacaa tctgccaatt tccagttaca tacgcctcaa ggttcaagtt ttgtaagact gccgtttgcg ggtgagcaca atgtgcaaaa cgcaactgca gcagtagcat ttgctttggc gttaggtgtt agcttggaag atatcgtaaa aggtctagag caagcacaag gtgcaaaagg tcgtttgaat tttattcaaa aaacgcctca tttatttatt gatgatactt ataacgccaa tccaacatct atgcgtgctg cggcacaagt attacttcag caaaatggca ttaaagtcat ggtgatgggg gatattgggg agttaggtga tagcagttgg caagaacatc acgatttagg tcgcgatctt gctgagcttc ctttagacca tattgttgca gttgggcagt ttgcctcagc agctctcgaa ggcgcaggtc ttcattctac taaattgaaa gcatttcaga ctcaagctga ggcgcttcca tttttaatca atctaatcca aacacatcaa ccccagtcca tgagtttcct gtttaaaggt tctcgcttta ctcatatgga aacgttgatg gctgatttga tggagaaact ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat