AcbaC.00137.b
UDP-N-acetylmuramate--alanine ligase (MurC)
| CENTER ID: | AcbaC.00137.b |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00137.b.B4.GE40443 | truncation 3 | 10 | 482 | ||
| AcbaC.00137.b.B5.GE40444 | truncation 4 | 19 | 482 | ||
| AcbaC.00137.b.B6.GE40445 | truncation 5 | 29 | 482 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00137.b.B6.PS38212 | truncation 5 | 29 | 482 |
Structures
6CAU
DEPOSITED: 1/31/2018
DETERMINATION: XRay
CLONE: AcbaC.00137.b.B5.GE40444
PROTEIN: AcbaC.00137.b.B5.PD00479
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA29942.1 |
| PATRIC ID: | fig|470.1345.peg.143 |
| PGAT: | acinetobacter:ABUW_0147 |
| RefSeq: | WP_000075472.1 |
| UniProt: | V5V8V9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPTTAANQA KKLIKVPEMR RIKHIHFVGI GGAGMCGIAE VLANQGYKIS GSDIKASKTT QQLEENGIKV YIGHEAENIK NANVLVVSTA IDPENPEVKA AIEQRIPIVR RAEMLGELMR YRHGIAVAGT HGKTTTTSLL TTMLAEENLD PTYVIGGLLN STGVNAALGE SRFIVAEADE SDASFLYLQP MAAIVTNIDA DHMDTYEGSF DKLKDTFVQF LHNLPFYGLA VVCGDDANIR EILPRVGRPV ITYGFNEDND IRAIDVEQDG MRSHFTVLRK GREPLRLTIN QPGLHNVLNA LAAIGVATDE GVSDEAISRA LKGFSGVGRR FQVQGEFELG EGNVKLVDDY GHHPKEVEAT IKAARQSHPD RRLVMLFQPH RYSRTRDCFD DFIEVLSQVD QLLLLEVYPA GEKPIVGADS RTLARSIRLR GQVEPILIDP VEGNLQNIMQ NVLQPNDLLL TQGAGNVGAI SVELAQHHLY VK
NT Sequence
ATGTCTCCAA CAACAGCTGC GAACCAAGCA AAAAAACTGA TTAAAGTGCC TGAAATGCGC CGTATCAAAC ACATTCATTT TGTGGGAATT GGCGGTGCAG GTATGTGTGG TATTGCTGAG GTGTTAGCTA ACCAAGGATA TAAAATTTCC GGTTCAGATA TTAAGGCATC AAAAACCACA CAACAATTAG AAGAAAATGG CATTAAGGTC TATATCGGAC ACGAAGCAGA AAATATTAAA AATGCAAATG TGCTTGTCGT GTCAACAGCA ATTGACCCGG AAAATCCGGA AGTTAAAGCT GCCATTGAAC AACGTATTCC GATTGTACGT CGTGCAGAAA TGCTCGGTGA ACTTATGCGT TACCGTCATG GTATTGCTGT GGCAGGTACA CATGGTAAAA CTACAACCAC AAGTCTTTTG ACGACAATGT TGGCGGAAGA AAACCTCGAC CCAACTTATG TGATCGGTGG TTTACTTAAC AGTACAGGCG TAAATGCAGC ACTGGGTGAA AGCCGCTTTA TTGTTGCTGA AGCTGACGAA TCGGATGCAT CTTTCCTTTA TTTACAGCCA ATGGCAGCGA TTGTAACCAA CATTGATGCT GACCATATGG ATACTTATGA AGGCAGTTTC GATAAATTAA AAGATACCTT CGTACAGTTC CTTCATAACT TACCATTCTA CGGCTTGGCT GTGGTTTGTG GCGATGATGC GAACATCCGC GAGATTTTGC CACGTGTAGG CCGTCCGGTT ATTACTTATG GTTTTAATGA AGATAATGAC ATCCGTGCTA TCGATGTAGA GCAAGATGGT ATGCGTTCGC ACTTTACTGT TTTACGTAAA GGACGTGAGC CGTTACGTCT AACTATTAAT CAACCGGGTT TACATAACGT TTTAAATGCT TTGGCTGCAA TTGGTGTTGC AACTGATGAA GGCGTTTCGG ATGAAGCAAT TAGCCGTGCA TTAAAAGGTT TTAGTGGTGT TGGACGTCGC TTCCAAGTAC AAGGTGAATT TGAACTTGGT GAAGGGAATG TAAAATTAGT CGATGACTAT GGGCATCATC CTAAAGAAGT GGAAGCTACG ATTAAAGCTG CTCGTCAGAG CCATCCTGAT CGTCGTTTAG TTATGCTTTT CCAACCACAC CGTTACTCTC GTACACGTGA TTGTTTTGAT GACTTTATTG AAGTTTTATC TCAAGTCGAC CAATTATTAT TACTAGAAGT TTATCCGGCT GGGGAAAAAC CAATTGTTGG CGCAGATAGC CGTACTTTGG CGCGTAGTAT TCGCTTGCGT GGTCAGGTTG AACCGATTTT GATTGATCCG GTTGAAGGTA ATTTGCAAAA CATCATGCAA AATGTGTTAC AACCAAATGA CTTGTTATTA ACGCAAGGTG CAGGTAACGT GGGAGCAATT TCAGTAGAAC TTGCACAGCA CCATTTGTAT GTGAAATAA
Details for AcbaC.00137.b.B4.GE40443
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAK KLIKVPEMRR IKHIHFVGIG GAGMCGIAEV LANQGYKISG SDIKASKTTQ QLEENGIKVY IGHEAENIKN ANVLVVSTAI DPENPEVKAA IEQRIPIVRR AEMLGELMRY RHGIAVAGTH GKTTTTSLLT TMLAEENLDP TYVIGGLLNS TGVNAALGES RFIVAEADES DASFLYLQPM AAIVTNIDAD HMDTYEGSFD KLKDTFVQFL HNLPFYGLAV VCGDDANIRE ILPRVGRPVI TYGFNEDNDI RAIDVEQDGM RSHFTVLRKG REPLRLTINQ PGLHNVLNAL AAIGVATDEG VSDEAISRAL KGFSGVGRRF QVQGEFELGE GNVKLVDDYG HHPKEVEATI KAARQSHPDR RLVMLFQPHR YSRTRDCFDD FIEVLSQVDQ LLLLEVYPAG EKPIVGADSR TLARSIRLRG QVEPILIDPV EGNLQNIMQN VLQPNDLLLT QGAGNVGAIS VELAQHHLYV K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaaaaa aactgattaa agtgcctgaa atgcgccgta tcaaacacat tcattttgtg ggaattggcg gtgcaggtat gtgtggtatt gctgaggtgt tagctaacca aggatataaa atttccggtt cagatattaa ggcatcaaaa accacacaac aattagaaga aaatggcatt aaggtctata tcggacacga agcagaaaat attaaaaatg caaatgtgct tgtcgtgtca acagcaattg acccggaaaa tccggaagtt aaagctgcca ttgaacaacg tattccgatt gtacgtcgtg cagaaatgct cggtgaactt atgcgttacc gtcatggtat tgctgtggca ggtacacatg gtaaaactac aaccacaagt cttttgacga caatgttggc ggaagaaaac ctcgacccaa cttatgtgat cggtggttta cttaacagta caggcgtaaa tgcagcactg ggtgaaagcc gctttattgt tgctgaagct gacgaatcgg atgcatcttt cctttattta cagccaatgg cagcgattgt aaccaacatt gatgctgacc atatggatac ttatgaaggc agtttcgata aattaaaaga taccttcgta cagttccttc ataacttacc attctacggc ttggctgtgg tttgtggcga tgatgcgaac atccgcgaga ttttgccacg tgtaggccgt ccggttatta cttatggttt taatgaagat aatgacatcc gtgctatcga tgtagagcaa gatggtatgc gttcgcactt tactgtttta cgtaaaggac gtgagccgtt acgtctaact attaatcaac cgggtttaca taacgtttta aatgctttgg ctgcaattgg tgttgcaact gatgaaggcg tttcggatga agcaattagc cgtgcattaa aaggttttag tggtgttgga cgtcgcttcc aagtacaagg tgaatttgaa cttggtgaag ggaatgtaaa attagtcgat gactatgggc atcatcctaa agaagtggaa gctacgatta aagctgctcg tcagagccat cctgatcgtc gtttagttat gcttttccaa ccacaccgtt actctcgtac acgtgattgt tttgatgact ttattgaagt tttatctcaa gtcgaccaat tattattact agaagtttat ccggctgggg aaaaaccaat tgttggcgca gatagccgta ctttggcgcg tagtattcgc ttgcgtggtc aggttgaacc gattttgatt gatccggttg aaggtaattt gcaaaacatc atgcaaaatg tgttacaacc aaatgacttg ttattaacgc aaggtgcagg taacgtggga gcaatttcag tagaacttgc acagcaccat ttgtatgtga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for AcbaC.00137.b.B5.GE40444
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR RIKHIHFVGI GGAGMCGIAE VLANQGYKIS GSDIKASKTT QQLEENGIKV YIGHEAENIK NANVLVVSTA IDPENPEVKA AIEQRIPIVR RAEMLGELMR YRHGIAVAGT HGKTTTTSLL TTMLAEENLD PTYVIGGLLN STGVNAALGE SRFIVAEADE SDASFLYLQP MAAIVTNIDA DHMDTYEGSF DKLKDTFVQF LHNLPFYGLA VVCGDDANIR EILPRVGRPV ITYGFNEDND IRAIDVEQDG MRSHFTVLRK GREPLRLTIN QPGLHNVLNA LAAIGVATDE GVSDEAISRA LKGFSGVGRR FQVQGEFELG EGNVKLVDDY GHHPKEVEAT IKAARQSHPD RRLVMLFQPH RYSRTRDCFD DFIEVLSQVD QLLLLEVYPA GEKPIVGADS RTLARSIRLR GQVEPILIDP VEGNLQNIMQ NVLQPNDLLL TQGAGNVGAI SVELAQHHLY VK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gtatcaaaca cattcatttt gtgggaattg gcggtgcagg tatgtgtggt attgctgagg tgttagctaa ccaaggatat aaaatttccg gttcagatat taaggcatca aaaaccacac aacaattaga agaaaatggc attaaggtct atatcggaca cgaagcagaa aatattaaaa atgcaaatgt gcttgtcgtg tcaacagcaa ttgacccgga aaatccggaa gttaaagctg ccattgaaca acgtattccg attgtacgtc gtgcagaaat gctcggtgaa cttatgcgtt accgtcatgg tattgctgtg gcaggtacac atggtaaaac tacaaccaca agtcttttga cgacaatgtt ggcggaagaa aacctcgacc caacttatgt gatcggtggt ttacttaaca gtacaggcgt aaatgcagca ctgggtgaaa gccgctttat tgttgctgaa gctgacgaat cggatgcatc tttcctttat ttacagccaa tggcagcgat tgtaaccaac attgatgctg accatatgga tacttatgaa ggcagtttcg ataaattaaa agataccttc gtacagttcc ttcataactt accattctac ggcttggctg tggtttgtgg cgatgatgcg aacatccgcg agattttgcc acgtgtaggc cgtccggtta ttacttatgg ttttaatgaa gataatgaca tccgtgctat cgatgtagag caagatggta tgcgttcgca ctttactgtt ttacgtaaag gacgtgagcc gttacgtcta actattaatc aaccgggttt acataacgtt ttaaatgctt tggctgcaat tggtgttgca actgatgaag gcgtttcgga tgaagcaatt agccgtgcat taaaaggttt tagtggtgtt ggacgtcgct tccaagtaca aggtgaattt gaacttggtg aagggaatgt aaaattagtc gatgactatg ggcatcatcc taaagaagtg gaagctacga ttaaagctgc tcgtcagagc catcctgatc gtcgtttagt tatgcttttc caaccacacc gttactctcg tacacgtgat tgttttgatg actttattga agttttatct caagtcgacc aattattatt actagaagtt tatccggctg gggaaaaacc aattgttggc gcagatagcc gtactttggc gcgtagtatt cgcttgcgtg gtcaggttga accgattttg attgatccgg ttgaaggtaa tttgcaaaac atcatgcaaa atgtgttaca accaaatgac ttgttattaa cgcaaggtgc aggtaacgtg ggagcaattt cagtagaact tgcacagcac catttgtatg tgaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.00137.b.B6.GE40445
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGI GGAGMCGIAE VLANQGYKIS GSDIKASKTT QQLEENGIKV YIGHEAENIK NANVLVVSTA IDPENPEVKA AIEQRIPIVR RAEMLGELMR YRHGIAVAGT HGKTTTTSLL TTMLAEENLD PTYVIGGLLN STGVNAALGE SRFIVAEADE SDASFLYLQP MAAIVTNIDA DHMDTYEGSF DKLKDTFVQF LHNLPFYGLA VVCGDDANIR EILPRVGRPV ITYGFNEDND IRAIDVEQDG MRSHFTVLRK GREPLRLTIN QPGLHNVLNA LAAIGVATDE GVSDEAISRA LKGFSGVGRR FQVQGEFELG EGNVKLVDDY GHHPKEVEAT IKAARQSHPD RRLVMLFQPH RYSRTRDCFD DFIEVLSQVD QLLLLEVYPA GEKPIVGADS RTLARSIRLR GQVEPILIDP VEGNLQNIMQ NVLQPNDLLL TQGAGNVGAI SVELAQHHLY VK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggaattg gcggtgcagg tatgtgtggt attgctgagg tgttagctaa ccaaggatat aaaatttccg gttcagatat taaggcatca aaaaccacac aacaattaga agaaaatggc attaaggtct atatcggaca cgaagcagaa aatattaaaa atgcaaatgt gcttgtcgtg tcaacagcaa ttgacccgga aaatccggaa gttaaagctg ccattgaaca acgtattccg attgtacgtc gtgcagaaat gctcggtgaa cttatgcgtt accgtcatgg tattgctgtg gcaggtacac atggtaaaac tacaaccaca agtcttttga cgacaatgtt ggcggaagaa aacctcgacc caacttatgt gatcggtggt ttacttaaca gtacaggcgt aaatgcagca ctgggtgaaa gccgctttat tgttgctgaa gctgacgaat cggatgcatc tttcctttat ttacagccaa tggcagcgat tgtaaccaac attgatgctg accatatgga tacttatgaa ggcagtttcg ataaattaaa agataccttc gtacagttcc ttcataactt accattctac ggcttggctg tggtttgtgg cgatgatgcg aacatccgcg agattttgcc acgtgtaggc cgtccggtta ttacttatgg ttttaatgaa gataatgaca tccgtgctat cgatgtagag caagatggta tgcgttcgca ctttactgtt ttacgtaaag gacgtgagcc gttacgtcta actattaatc aaccgggttt acataacgtt ttaaatgctt tggctgcaat tggtgttgca actgatgaag gcgtttcgga tgaagcaatt agccgtgcat taaaaggttt tagtggtgtt ggacgtcgct tccaagtaca aggtgaattt gaacttggtg aagggaatgt aaaattagtc gatgactatg ggcatcatcc taaagaagtg gaagctacga ttaaagctgc tcgtcagagc catcctgatc gtcgtttagt tatgcttttc caaccacacc gttactctcg tacacgtgat tgttttgatg actttattga agttttatct caagtcgacc aattattatt actagaagtt tatccggctg gggaaaaacc aattgttggc gcagatagcc gtactttggc gcgtagtatt cgcttgcgtg gtcaggttga accgattttg attgatccgg ttgaaggtaa tttgcaaaac atcatgcaaa atgtgttaca accaaatgac ttgttattaa cgcaaggtgc aggtaacgtg ggagcaattt cagtagaact tgcacagcac catttgtatg tgaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.00137.b.B6.PS38212
| PURIFICATION DATe: | 2/28/2017 |
| CONCENTRATION: | 29.8mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer:20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHGI GGAGMCGIAE VLANQGYKIS GSDIKASKTT QQLEENGIKV YIGHEAENIK NANVLVVSTA IDPENPEVKA AIEQRIPIVR RAEMLGELMR YRHGIAVAGT HGKTTTTSLL TTMLAEENLD PTYVIGGLLN STGVNAALGE SRFIVAEADE SDASFLYLQP MAAIVTNIDA DHMDTYEGSF DKLKDTFVQF LHNLPFYGLA VVCGDDANIR EILPRVGRPV ITYGFNEDND IRAIDVEQDG MRSHFTVLRK GREPLRLTIN QPGLHNVLNA LAAIGVATDE GVSDEAISRA LKGFSGVGRR FQVQGEFELG EGNVKLVDDY GHHPKEVEAT IKAARQSHPD RRLVMLFQPH RYSRTRDCFD DFIEVLSQVD QLLLLEVYPA GEKPIVGADS RTLARSIRLR GQVEPILIDP VEGNLQNIMQ NVLQPNDLLL TQGAGNVGAI SVELAQHHLY VK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGAATTG GCGGTGCAGG TATGTGTGGT ATTGCTGAGG TGTTAGCTAA CCAAGGATAT AAAATTTCCG GTTCAGATAT TAAGGCATCA AAAACCACAC AACAATTAGA AGAAAATGGC ATTAAGGTCT ATATCGGACA CGAAGCAGAA AATATTAAAA ATGCAAATGT GCTTGTCGTG TCAACAGCAA TTGACCCGGA AAATCCGGAA GTTAAAGCTG CCATTGAACA ACGTATTCCG ATTGTACGTC GTGCAGAAAT GCTCGGTGAA CTTATGCGTT ACCGTCATGG TATTGCTGTG GCAGGTACAC ATGGTAAAAC TACAACCACA AGTCTTTTGA CGACAATGTT GGCGGAAGAA AACCTCGACC CAACTTATGT GATCGGTGGT TTACTTAACA GTACAGGCGT AAATGCAGCA CTGGGTGAAA GCCGCTTTAT TGTTGCTGAA GCTGACGAAT CGGATGCATC TTTCCTTTAT TTACAGCCAA TGGCAGCGAT TGTAACCAAC ATTGATGCTG ACCATATGGA TACTTATGAA GGCAGTTTCG ATAAATTAAA AGATACCTTC GTACAGTTCC TTCATAACTT ACCATTCTAC GGCTTGGCTG TGGTTTGTGG CGATGATGCG AACATCCGCG AGATTTTGCC ACGTGTAGGC CGTCCGGTTA TTACTTATGG TTTTAATGAA GATAATGACA TCCGTGCTAT CGATGTAGAG CAAGATGGTA TGCGTTCGCA CTTTACTGTT TTACGTAAAG GACGTGAGCC GTTACGTCTA ACTATTAATC AACCGGGTTT ACATAACGTT TTAAATGCTT TGGCTGCAAT TGGTGTTGCA ACTGATGAAG GCGTTTCGGA TGAAGCAATT AGCCGTGCAT TAAAAGGTTT TAGTGGTGTT GGACGTCGCT TCCAAGTACA AGGTGAATTT GAACTTGGTG AAGGGAATGT AAAATTAGTC GATGACTATG GGCATCATCC TAAAGAAGTG GAAGCTACGA TTAAAGCTGC TCGTCAGAGC CATCCTGATC GTCGTTTAGT TATGCTTTTC CAACCACACC GTTACTCTCG TACACGTGAT TGTTTTGATG ACTTTATTGA AGTTTTATCT CAAGTCGACC AATTATTATT ACTAGAAGTT TATCCGGCTG GGGAAAAACC AATTGTTGGC GCAGATAGCC GTACTTTGGC GCGTAGTATT CGCTTGCGTG GTCAGGTTGA ACCGATTTTG ATTGATCCGG TTGAAGGTAA TTTGCAAAAC ATCATGCAAA ATGTGTTACA ACCAAATGAC TTGTTATTAA CGCAAGGTGC AGGTAACGTG GGAGCAATTT CAGTAGAACT TGCACAGCAC CATTTGTATG TGAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT