SSGCID: Target Page

AcbaC.00133.a

cysteinyl-tRNA synthetase

CENTER ID:	AcbaC.00133.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00133.a.B1.GE38590	full length		1	473
AcbaC.00133.a.B5.GE40442	truncation 4		6	470

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00133.a.B1.PS02345	full length		1	473

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.2604
GenBank:	AKA32372.1
PGAT:	acinetobacter:ABUW_2650
RefSeq:	WP_001182272.1
UniProt:	A0A0D5YJE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQPFVLYNSE QRKKVEFVPR KEGHIDMYVC GMTVYDYCHI GHARVMVAFD YIIRFLRSQG WKVRYIRNIT DIDDKIIKRA NENGETIQQL TTRFIDAMNE DAANLGCLAP DEAPKATEYI DQMQNMIGNL VNKGAAYPAS NGDVYFEVTK FEKYGRLSGR KLDDMQAGAS ERIDVEVEKK HPFDFVLWKH AKENEPSWAS PWGNGRPGWH IECSAMSTCC LGNHFDIHGG GSDLMFPHHE NEIAQSEAST GEQYVNYWMH VGFINVDGEK MSKSLGNFFT IRDVMEKFHP EVIRYFIVSS HYRSPVNFSD VALKEAKTSL TRFYHSFKAY QQVYGQTTTE ALDQSFIERF NNAMCDDFNT AEAMAVLFEL NKELNRAVKE EQADQATVLY STLRHLTNIL GLVQHNVDDF LKSDIGQDAL ALSDAEIEDF IQQRVDAKKA KDFAKADSIR QSLLEQGVVL EDTRQGTVWR RAD

NT Sequence

ATGCAACCGT TTGTTTTATA TAACTCTGAG CAACGAAAAA AAGTTGAATT TGTACCTCGC AAAGAAGGTC ACATCGATAT GTACGTCTGC GGTATGACCG TTTACGACTA CTGTCATATC GGGCATGCTC GAGTTATGGT TGCATTTGAC TACATTATCC GTTTTTTGCG TAGTCAAGGC TGGAAAGTCC GCTACATTCG CAACATTACC GATATCGATG ACAAAATCAT CAAACGTGCG AATGAGAATG GTGAAACAAT CCAACAGCTC ACCACGCGTT TCATCGATGC CATGAATGAA GATGCAGCGA ACTTAGGCTG TTTAGCACCA GATGAAGCAC CTAAAGCGAC TGAATATATT GACCAGATGC AAAATATGAT TGGTAATCTG GTAAATAAAG GCGCTGCTTA CCCTGCTTCA AACGGTGATG TGTATTTTGA AGTCACCAAA TTTGAAAAAT ATGGACGTCT CTCTGGCCGT AAGCTTGATG ACATGCAAGC TGGCGCAAGT GAGCGTATTG ATGTAGAAGT TGAAAAGAAA CATCCTTTTG ACTTTGTGCT TTGGAAACAT GCAAAAGAAA ATGAACCGTC TTGGGCATCC CCTTGGGGTA ATGGTCGTCC GGGTTGGCAC ATTGAATGTT CTGCAATGTC GACTTGCTGC CTAGGCAATC ACTTTGACAT TCATGGTGGC GGTTCAGATT TAATGTTCCC GCACCATGAA AATGAAATTG CGCAAAGTGA AGCTTCGACT GGTGAGCAAT ATGTAAACTA CTGGATGCAT GTTGGCTTTA TTAACGTTGA TGGTGAAAAG ATGTCTAAGT CTTTAGGCAA CTTCTTTACG ATTCGTGACG TAATGGAGAA ATTCCACCCA GAAGTGATTC GATACTTCAT TGTGTCTTCA CACTATCGTA GCCCTGTGAA CTTCTCTGAT GTAGCACTTA AAGAGGCAAA AACTTCTTTA ACTCGTTTCT ATCATTCATT TAAAGCTTAC CAACAAGTGT ACGGTCAAAC GACAACTGAA GCGCTTGATC AAAGCTTTAT TGAACGCTTT AACAATGCAA TGTGTGACGA TTTCAATACT GCCGAAGCAA TGGCTGTATT GTTTGAACTG AACAAAGAGT TAAACCGTGC TGTAAAAGAA GAGCAAGCTG ACCAAGCGAC TGTGCTTTAT TCGACATTAC GTCACCTCAC CAACATTTTA GGTTTGGTAC AACACAATGT AGACGATTTC TTAAAATCAG ATATTGGACA AGATGCACTT GCTTTGTCTG ATGCTGAAAT TGAAGATTTC ATTCAACAAC GTGTTGATGC GAAAAAAGCA AAAGACTTTG CTAAAGCAGA TAGCATTCGT CAGTCTTTAC TTGAGCAAGG CGTAGTTCTT GAAGACACTC GTCAAGGTAC AGTTTGGCGT CGTGCTGATT AA

Details for AcbaC.00133.a.B1.GE38590

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ PFVLYNSEQR KKVEFVPRKE GHIDMYVCGM TVYDYCHIGH ARVMVAFDYI IRFLRSQGWK VRYIRNITDI DDKIIKRANE NGETIQQLTT RFIDAMNEDA ANLGCLAPDE APKATEYIDQ MQNMIGNLVN KGAAYPASNG DVYFEVTKFE KYGRLSGRKL DDMQAGASER IDVEVEKKHP FDFVLWKHAK ENEPSWASPW GNGRPGWHIE CSAMSTCCLG NHFDIHGGGS DLMFPHHENE IAQSEASTGE QYVNYWMHVG FINVDGEKMS KSLGNFFTIR DVMEKFHPEV IRYFIVSSHY RSPVNFSDVA LKEAKTSLTR FYHSFKAYQQ VYGQTTTEAL DQSFIERFNN AMCDDFNTAE AMAVLFELNK ELNRAVKEEQ ADQATVLYST LRHLTNILGL VQHNVDDFLK SDIGQDALAL SDAEIEDFIQ QRVDAKKAKD FAKADSIRQS LLEQGVVLED TRQ

Validated NT Sequence

cttgacgagt gtcttcaaga actacgcctt gctcaagtaa agactgacga atgctatctg ctttagcaaa gtcttttgct tttttcgcat caacacgttg ttgaatgaaa tcttcaattt cagcatcaga caaagcaagt gcatcttgtc caatatctga ttttaagaaa tcgtctacat tgtgttgtac caaacctaaa atgttggtga ggtgacgtaa tgtcgaataa agcacagtcg cttggtcagc ttgctcttct tttacagcac ggtttaactc tttgttcagt tcaaacaata cagccattgc ttcggcagta ttgaaatcgt cacacattgc attgttaaag cgttcaataa agctttgatc aagcgcttca gttgtcgttt gaccgtacac ttgttggtaa gctttaaatg aatgatagaa acgagttaaa gaagtttttg cctctttaag tgctacatca gagaagttca cagggctacg atagtgtgaa gacacaatga agtatcgaat cacttctggg tggaatttct ccattacgtc acgaatcgta aagaagttgc ctaaagactt agacatcttt tcaccatcaa cgttaataaa gccaacatgc atccagtagt ttacatattg ctcaccagtc gaagcttcac tttgcgcaat ttcattttca tggtgcggga acattaaatc tgaaccgcca ccatgaatgt caaagtgatt gcctaggcag caagtcgaca ttgcagaaca ttcaatgtgc caacccggac gaccattacc ccaaggggat gcccaagacg gttcattttc ttttgcatgt ttccaaagca caaagtcaaa aggatgtttc ttttcaactt ctacatcaat acgctcactt gcgccagctt gcatgtcatc aagcttacgg ccagagagac gtccatattt ttcaaatttg gtgacttcaa aatacacatc accgtttgaa gcagggtaag cagcgccttt atttaccaga ttaccaatca tattttgcat ctggtcaata tattcagtcg ctttaggtgc ttcatctggt gctaaacagc ctaagttcgc tgcatcttca ttcatggcat cgatgaaacg cgtggtgagc tgttggattg tttcaccatt ctcattcgca cgtttgatga ttttgtcatc gatatcggta atgttgcgaa tgtagcggac tttccagcct tgactacgca aaaaacggat aatgtagtca aatgcaacca taactcgagc atgcccgata tgacagtagt cgtaaacggt cataccgcag acgtacatat cgatgtgacc ttctttgcga ggtacaaatt caactttttt tcgttgctca gagttatata aaacaaacgg ttgcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac cgtttgtttt atataactct gagcaacgaa aaaaagttga atttgtacct cgcaaagaag gtcacatcga tatgtacgtc tgcggtatga ccgtttacga ctactgtcat atcgggcatg ctcgagttat ggttgcattt gactacatta tccgtttttt gcgtagtcaa ggctggaaag tccgctacat tcgcaacatt accgatatcg atgacaaaat catcaaacgt gcgaatgaga atggtgaaac aatccaacag ctcaccacgc gtttcatcga tgccatgaat gaagatgcag cgaacttagg ctgtttagca ccagatgaag cacctaaagc gactgaatat attgaccaga tgcaaaatat gattggtaat ctggtaaata aaggcgctgc ttaccctgct tcaaacggtg atgtgtattt tgaagtcacc aaatttgaaa aatatggacg tctctctggc cgtaagcttg atgacatgca agctggcgca agtgagcgta ttgatgtaga agttgaaaag aaacatcctt ttgactttgt gctttggaaa catgcaaaag aaaatgaacc gtcttgggca tccccttggg gtaatggtcg tccgggttgg cacattgaat gttctgcaat gtcgacttgc tgcctaggca atcactttga cattcatggt ggcggttcag atttaatgtt cccgcaccat gaaaatgaaa ttgcgcaaag tgaagcttcg actggtgagc aatatgtaaa ctactggatg catgttggct ttattaacgt tgatggtgaa aagatgtcta agtctttagg caacttcttt acgattcgtg acgtaatgga gaaattccac ccagaagtga ttcgatactt cattgtgtct tcacactatc gtagccctgt gaacttctct gatgtagcac ttaaagaggc aaaaacttct ttaactcgtt tctatcattc atttaaagct taccaacaag tgtacggtca aacgacaact gaagcgcttg atcaaagctt tattgaacgc tttaacaatg caatgtgtga cgatttcaat actgccgaag caatggctgt attgtttgaa ctgaacaaag agttaaaccg tgctgtaaaa gaagagcaag ctgaccaagc gactgtgctt tattcgacat tacgtcacct caccaacatt ttaggtttgg tacaacacaa tgtagacgat ttcttaaaat cagatattgg acaagatgca cttgctttgt ctgatgctga aattgaagat ttcattcaac aacgtgttga tgcgaaaaaa gcaaaagact ttgctaaagc agatagcatt cgtcagtctt tacttgagca aggcgtagtt cttgaagaca ctcgtcaagg tacagtttgg cgtcgtgctg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for AcbaC.00133.a.B5.GE40442

HARVESTED ON:	8/10/2016
SEQUENCED ON:	8/11/2016
EXPECTED MW:	54kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLY NSEQRKKVEF VPRKEGHIDM YVCGMTVYDY CHIGHARVMV AFDYIIRFLR SQGWKVRYIR NITDIDDKII KRANENGETI QQLTTRFIDA MNEDAANLGC LAPDEAPKAT EYIDQMQNMI GNLVNKGAAY PASNGDVYFE VTKFEKYGRL SGRKLDDMQA GASERIDVEV EKKHPFDFVL WKHAKENEPS WASPWGNGRP GWHIECSAMS TCCLGNHFDI HGGGSDLMFP HHENEIAQSE ASTGEQYVNY WMHVGFINVD GEKMSKSLGN FFTIRDVMEK FHPEVIRYFI VSSHYRSPVN FSDVALKEAK TSLTRFYHSF KAYQQVYGQT TTEALDQSFI ERFNNAMCDD FNTAEAMAVL FELNKELNRA VKEEQADQAT VLYSTLRHLT NILGLVQHNV DDFLKSDIGQ DALALSDAEI EDFIQQRVDA KKAKDFAKAD SIRQSLLEQG VVLEDTRQGT VWR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttatata actctgagca acgaaaaaaa gttgaatttg tacctcgcaa agaaggtcac atcgatatgt acgtctgcgg tatgaccgtt tacgactact gtcatatcgg gcatgctcga gttatggttg catttgacta cattatccgt tttttgcgta gtcaaggctg gaaagtccgc tacattcgca acattaccga tatcgatgac aaaatcatca aacgtgcgaa tgagaatggt gaaacaatcc aacagctcac cacgcgtttc atcgatgcca tgaatgaaga tgcagcgaac ttaggctgtt tagcaccaga tgaagcacct aaagcgactg aatatattga ccagatgcaa aatatgattg gtaatctggt aaataaaggc gctgcttacc ctgcttcaaa cggtgatgtg tattttgaag tcaccaaatt tgaaaaatat ggacgtctct ctggccgtaa gcttgatgac atgcaagctg gcgcaagtga gcgtattgat gtagaagttg aaaagaaaca tccttttgac tttgtgcttt ggaaacatgc aaaagaaaat gaaccgtctt gggcatcccc ttggggtaat ggtcgtccgg gttggcacat tgaatgttct gcaatgtcga cttgctgcct aggcaatcac tttgacattc atggtggcgg ttcagattta atgttcccgc accatgaaaa tgaaattgcg caaagtgaag cttcgactgg tgagcaatat gtaaactact ggatgcatgt tggctttatt aacgttgatg gtgaaaagat gtctaagtct ttaggcaact tctttacgat tcgtgacgta atggagaaat tccacccaga agtgattcga tacttcattg tgtcttcaca ctatcgtagc cctgtgaact tctctgatgt agcacttaaa gaggcaaaaa cttctttaac tcgtttctat cattcattta aagcttacca acaagtgtac ggtcaaacga caactgaagc gcttgatcaa agctttattg aacgctttaa caatgcaatg tgtgacgatt tcaatactgc cgaagcaatg gctgtattgt ttgaactgaa caaagagtta aaccgtgctg taaaagaaga gcaagctgac caagcgactg tgctttattc gacattacgt cacctcacca acattttagg tttggtacaa cacaatgtag acgatttctt aaaatcagat attggacaag atgcacttgc tttgtctgat gctgaaattg aagatttcat tcaacaacgt gttgatgcga aaaaagcaaa agactttgct aaagcagata gcattcgtca gtctttactt gagcaaggcg tagttcttga agacactcgt caaggtacag tttggcgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for AcbaC.00133.a.B1.PS02345

PURIFICATION DATe:	2/18/2015
CONCENTRATION:	60.6mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC CGTTTGTTTT ATATAACTCT GAGCAACGAA AAAAAGTTGA ATTTGTACCT CGCAAAGAAG GTCACATCGA TATGTACGTC TGCGGTATGA CCGTTTACGA CTACTGTCAT ATCGGGCATG CTCGAGTTAT GGTTGCATTT GACTACATTA TCCGTTTTTT GCGTAGTCAA GGCTGGAAAG TCCGCTACAT TCGCAACATT ACCGATATCG ATGACAAAAT CATCAAACGT GCGAATGAGA ATGGTGAAAC AATCCAACAG CTCACCACGC GTTTCATCGA TGCCATGAAT GAAGATGCAG CGAACTTAGG CTGTTTAGCA CCAGATGAAG CACCTAAAGC GACTGAATAT ATTGACCAGA TGCAAAATAT GATTGGTAAT CTGGTAAATA AAGGCGCTGC TTACCCTGCT TCAAACGGTG ATGTGTATTT TGAAGTCACC AAATTTGAAA AATATGGACG TCTCTCTGGC CGTAAGCTTG ATGACATGCA AGCTGGCGCA AGTGAGCGTA TTGATGTAGA AGTTGAAAAG AAACATCCTT TTGACTTTGT GCTTTGGAAA CATGCAAAAG AAAATGAACC GTCTTGGGCA TCCCCTTGGG GTAATGGTCG TCCGGGTTGG CACATTGAAT GTTCTGCAAT GTCGACTTGC TGCCTAGGCA ATCACTTTGA CATTCATGGT GGCGGTTCAG ATTTAATGTT CCCGCACCAT GAAAATGAAA TTGCGCAAAG TGAAGCTTCG ACTGGTGAGC AATATGTAAA CTACTGGATG CATGTTGGCT TTATTAACGT TGATGGTGAA AAGATGTCTA AGTCTTTAGG CAACTTCTTT ACGATTCGTG ACGTAATGGA GAAATTCCAC CCAGAAGTGA TTCGATACTT CATTGTGTCT TCACACTATC GTAGCCCTGT GAACTTCTCT GATGTAGCAC TTAAAGAGGC AAAAACTTCT TTAACTCGTT TCTATCATTC ATTTAAAGCT TACCAACAAG TGTACGGTCA AACGACAACT GAAGCGCTTG ATCAAAGCTT TATTGAACGC TTTAACAATG CAATGTGTGA CGATTTCAAT ACTGCCGAAG CAATGGCTGT ATTGTTTGAA CTGAACAAAG AGTTAAACCG TGCTGTAAAA GAAGAGCAAG CTGACCAAGC GACTGTGCTT TATTCGACAT TACGTCACCT CACCAACATT TTAGGTTTGG TACAACACAA TGTAGACGAT TTCTTAAAAT CAGATATTGG ACAAGATGCA CTTGCTTTGT CTGATGCTGA AATTGAAGAT TTCATTCAAC AACGTGTTGA TGCGAAAAAA GCAAAAGACT TTGCTAAAGC AGATAGCATT CGTCAGTCTT TACTTGAGCA AGGCGTAGTT CTTGAAGACA CTCGTCAAGG TACAGTTTGG CGTCGTGCTG ATTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT