AcbaC.00119.a

D-alanine--D-alanine ligase B

CENTER ID:	AcbaC.00119.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00119.a.B1.GE38588	full length		1	308

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00119.a.B1.PW37650	full length		1	308

External Resources

RESOURCE	REFERENCE ID
GenBank:	AKA29943.1
PATRIC ID:	fig\|470.1345.peg.144
PGAT:	acinetobacter:ABUW_0148
RefSeq:	WP_000063665.1
UniProt:	A0A0D5YDD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

VSNATKFGKV AVLLGGKSAE RAVSLDSGQA VLDALLRSGV QAEAFDPQNR SVTELVNYDR AFIVLHGRGG EDGQIQGVLE WLNIPYTGTG VQGSAIGMDK VKTKQIWQGS DLPTAPYRII TKETDLDSVI AELGLPVIIK PVHEGSSVGM SKVEKAEDFA AAIEKATQHD AVVMAEKWIT GREFTISFLN GQPLPVIRLQ PPADVAFYDY EAKYQRNDVE YGIPCGLSET EEKKLQALCL RAFQAVGAEG WGRIDAMQDE QGNFWLLEVN TVPGMTSHSL VPKAAKAVGY SFDELCVAIL DQTLEGTA

NT Sequence

GTGTCAAATG CTACAAAATT CGGCAAAGTT GCCGTGTTAC TTGGTGGGAA ATCGGCTGAG CGTGCTGTGT CTTTAGATAG TGGTCAGGCA GTTTTGGACG CCTTGTTACG TTCAGGTGTT CAGGCTGAGG CATTTGATCC GCAAAACCGT AGCGTAACTG AACTTGTAAA CTATGACCGT GCATTTATTG TATTGCATGG TCGTGGTGGT GAAGACGGTC AAATCCAAGG TGTGCTTGAG TGGTTGAACA TCCCTTATAC AGGAACGGGT GTACAAGGTT CTGCCATTGG TATGGATAAA GTCAAAACCA AGCAAATCTG GCAAGGTAGT GATTTACCAA CCGCACCGTA TCGCATTATT ACTAAAGAAA CTGACTTGGA TTCAGTGATT GCTGAATTGG GGTTGCCTGT CATTATCAAG CCTGTACATG AAGGTTCAAG TGTAGGCATG AGTAAGGTTG AGAAAGCTGA AGATTTTGCG GCAGCAATTG AAAAGGCAAC TCAGCACGAT GCTGTAGTTA TGGCAGAAAA GTGGATTACA GGACGCGAAT TTACGATTTC ATTCTTAAAT GGTCAGCCTC TACCTGTTAT TCGTTTGCAG CCACCAGCAG ATGTTGCTTT CTATGACTAC GAAGCAAAAT ATCAACGTAA TGATGTTGAG TATGGCATTC CTTGTGGTCT TAGCGAAACT GAAGAGAAAA AACTACAAGC TCTATGTTTA CGCGCTTTTC AAGCCGTAGG TGCAGAAGGC TGGGGACGTA TTGATGCAAT GCAGGATGAG CAAGGTAACT TCTGGCTTTT AGAAGTAAAT ACTGTGCCAG GCATGACCAG TCATTCGCTT GTACCAAAGG CTGCTAAAGC AGTAGGTTAT AGCTTTGATG AATTGTGTGT TGCAATTCTT GATCAAACAT TGGAAGGTAC GGCTTAA

Details for AcbaC.00119.a.B1.GE38588

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV SNATKFGKVA VLLGGKSAER AVSLDSGQAV LDALLRSGVQ AEAFDPQNRS VTELVNYDRA FIVLHGRGGE DGQIQGVLEW LNIPYTGTGV QGSAIGMDKV KTKQIWQGSD LPTAPYRIIT KETDLDSVIA ELGLPVIIKP VHEGSSVGMS KVEKAEDFAA AIEKATQHDA VVMAEKWITG REFTISFLNG QPLPVIRLQP PADVAFYDYE AKYQRNDVEY GIPCGLSETE EKKLQALCLR AFQAVGAEGW GRIDAMQDEQ GNFWLLEVNT VPGMTSHSLV PKAAKAVGYS FDELCVAILX XXXGRYGLS

Validated NT Sequence

cacttaagcc gtaccttccn nnnnnnnnnn naagaattgc aacacacaat tcatcaaagc tataacctac tgctttagca gcctttggta caagcgaatg actggtcatg cctggcacag tatttacttc taaaagccag aagttacctt gctcatcctg cattgcatca atacgtcccc agccttctgc acctacggct tgaaaagcgc gtaaacatag agcttgtagt tttttctctt cagtttcgct aagaccacaa ggaatgccat actcaacatc attacgttga tattttgctt cgtagtcata gaaagcaaca tctgctggtg gctgcaaacg aataacaggt agaggctgac catttaagaa tgaaatcgta aattcgcgtc ctgtaatcca cttttctgcc ataactacag catcgtgctg agttgccttt tcaattgctg ccgcaaaatc ttcagctttc tcaaccttac tcatgcctac acttgaacct tcatgtacag gcttgataat gacaggcaac cccaattcag caatcactga atccaagtca gtttctttag taataatgcg atacggtgcg gttggtaaat cactaccttg ccagatttgc ttggttttga ctttatccat accaatggca gaaccttgta cacccgttcc tgtataaggg atgttcaacc actcaagcac accttggatt tgaccgtctt caccaccacg accatgcaat acaataaatg cacggtcata gtttacaagt tcagttacgc tacggttttg cggatcaaat gcctcagcct gaacacctga acgtaacaag gcgtccaaaa ctgcctgacc actatctaaa gacacagcac gctcagccga tttcccacca agtaacacgg caactttgcc gaattttgta gcatttgaca ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHVS NATKFGKVAV LLGGKSAERA VSLDSGQAVL DALLRSGVQA EAFDPQNRSV TELVNYDRAF IVLHGRGGED GQIQGVLEWL NIPYTGTGVQ GSAIGMDKVK TKQIWQGSDL PTAPYRIITK ETDLDSVIAE LGLPVIIKPV HEGSSVGMSK VEKAEDFAAA IEKATQHDAV VMAEKWITGR EFTISFLNGQ PLPVIRLQPP ADVAFYDYEA KYQRNDVEYG IPCGLSETEE KKLQALCLRA FQAVGAEGWG RIDAMQDEQG NFWLLEVNTV PGMTSHSLVP KAAKAVGYSF DELCVAILDQ TLEGTA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcaa atgctacaaa attcggcaaa gttgccgtgt tacttggtgg gaaatcggct gagcgtgctg tgtctttaga tagtggtcag gcagttttgg acgccttgtt acgttcaggt gttcaggctg aggcatttga tccgcaaaac cgtagcgtaa ctgaacttgt aaactatgac cgtgcattta ttgtattgca tggtcgtggt ggtgaagacg gtcaaatcca aggtgtgctt gagtggttga acatccctta tacaggaacg ggtgtacaag gttctgccat tggtatggat aaagtcaaaa ccaagcaaat ctggcaaggt agtgatttac caaccgcacc gtatcgcatt attactaaag aaactgactt ggattcagtg attgctgaat tggggttgcc tgtcattatc aagcctgtac atgaaggttc aagtgtaggc atgagtaagg ttgagaaagc tgaagatttt gcggcagcaa ttgaaaaggc aactcagcac gatgctgtag ttatggcaga aaagtggatt acaggacgcg aatttacgat ttcattctta aatggtcagc ctctacctgt tattcgtttg cagccaccag cagatgttgc tttctatgac tacgaagcaa aatatcaacg taatgatgtt gagtatggca ttccttgtgg tcttagcgaa actgaagaga aaaaactaca agctctatgt ttacgcgctt ttcaagccgt aggtgcagaa ggctggggac gtattgatgc aatgcaggat gagcaaggta acttctggct tttagaagta aatactgtgc caggcatgac cagtcattcg cttgtaccaa aggctgctaa agcagtaggt tatagctttg atgaattgtg tgttgcaatt cttgatcaaa cattggaagg tacggcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for AcbaC.00119.a.B1.PW37650

PURIFICATION DATe:	2/9/2015
CONCENTRATION:	22mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	24
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTCAA ATGCTACAAA ATTCGGCAAA GTTGCCGTGT TACTTGGTGG GAAATCGGCT GAGCGTGCTG TGTCTTTAGA TAGTGGTCAG GCAGTTTTGG ACGCCTTGTT ACGTTCAGGT GTTCAGGCTG AGGCATTTGA TCCGCAAAAC CGTAGCGTAA CTGAACTTGT AAACTATGAC CGTGCATTTA TTGTATTGCA TGGTCGTGGT GGTGAAGACG GTCAAATCCA AGGTGTGCTT GAGTGGTTGA ACATCCCTTA TACAGGAACG GGTGTACAAG GTTCTGCCAT TGGTATGGAT AAAGTCAAAA CCAAGCAAAT CTGGCAAGGT AGTGATTTAC CAACCGCACC GTATCGCATT ATTACTAAAG AAACTGACTT GGATTCAGTG ATTGCTGAAT TGGGGTTGCC TGTCATTATC AAGCCTGTAC ATGAAGGTTC AAGTGTAGGC ATGAGTAAGG TTGAGAAAGC TGAAGATTTT GCGGCAGCAA TTGAAAAGGC AACTCAGCAC GATGCTGTAG TTATGGCAGA AAAGTGGATT ACAGGACGCG AATTTACGAT TTCATTCTTA AATGGTCAGC CTCTACCTGT TATTCGTTTG CAGCCACCAG CAGATGTTGC TTTCTATGAC TACGAAGCAA AATATCAACG TAATGATGTT GAGTATGGCA TTCCTTGTGG TCTTAGCGAA ACTGAAGAGA AAAAACTACA AGCTCTATGT TTACGCGCTT TTCAAGCCGT AGGTGCAGAA GGCTGGGGAC GTATTGATGC AATGCAGGAT GAGCAAGGTA ACTTCTGGCT TTTAGAAGTA AATACTGTGC CAGGCATGAC CAGTCATTCG CTTGTACCAA AGGCTGCTAA AGCAGTAGGT TATAGCTTTG ATGAATTGTG TGTTGCAATT CTTGATCAAA CATTGGAAGG TACGGCTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT