AcbaC.00116.a

4-hydroxythreonine-4-phosphate dehydrogenase

CENTER ID: AcbaC.00116.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00116.a.B1.GE38587 full length 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00116.a.B1.PS02359 full length 1 324
External Resources
RESOURCE REFERENCE ID
GenBank: AKA30272.1
PATRIC ID: fig|470.1345.peg.472
PGAT: acinetobacter:ABUW_0495
UniProt: A0A0D5YD85
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VIVLPLYVTS GEPAGIGPDI CLSLAKRVDE RPVVVLADRN LLQQRAQKLG LDIKFLEYSG QAESSLQGEL YIEHVPLETI VVDGQLNAAN AAYVLEQLRR SADYAMSGKS VGVATAPVQK SVINDAGILF SGHTEYYQEF AGVERVVMML ATKTLRVALA TTHLPLRDVA DAITKERLHQ VIDILLHDLK TKFKINDPRV LVCGLNPHAG EDGYLGREEI DTINPVLESY RSQGVKLSLS LPADTLFTPE HLKNADAVLA MYHDQGLPVL KSQGFGEAIN ITLGLPFIRT SVDHGTALSL AGTGLAKSSS LNVAVDLALS LAAS
NT Sequence
GTGATTGTGC TGCCTTTATA TGTCACTTCC GGTGAACCTG CCGGTATTGG TCCAGATATT TGTTTAAGTC TGGCAAAACG GGTTGATGAA CGTCCTGTTG TGGTTTTGGC TGATCGAAAT TTATTACAAC AACGCGCTCA AAAGCTTGGT CTAGACATTA AATTTTTAGA GTATAGCGGA CAAGCTGAAT CGTCTTTGCA AGGCGAGCTT TATATAGAAC ATGTACCCCT AGAAACTATA GTCGTTGATG GACAGCTTAA TGCTGCTAAT GCCGCTTATG TATTAGAGCA ATTACGTCGC TCAGCTGATT ATGCTATGTC TGGTAAGAGC GTCGGTGTAG CCACTGCTCC TGTTCAAAAG TCGGTGATTA ATGATGCAGG GATTTTATTT AGTGGACATA CTGAATATTA TCAAGAATTT GCAGGCGTAG AGCGTGTTGT TATGATGCTG GCAACGAAAA CGTTGCGGGT GGCTCTGGCG ACCACTCATT TACCATTAAG AGATGTTGCA GATGCAATTA CCAAAGAACG CTTGCATCAA GTGATTGATA TTTTACTTCA CGACTTAAAA ACCAAGTTTA AAATTAATGA TCCTCGAGTC TTAGTATGTG GGTTAAATCC TCATGCAGGC GAAGATGGTT ATTTGGGACG TGAAGAGATT GATACGATCA ATCCGGTACT TGAGAGCTAC CGGTCACAGG GAGTTAAATT AAGTTTAAGT TTGCCAGCTG ATACTTTATT TACGCCTGAA CATCTTAAAA ATGCTGACGC TGTTCTTGCG ATGTACCATG ATCAAGGCTT ACCTGTGTTA AAATCACAGG GTTTTGGTGA AGCAATCAAT ATCACATTGG GCTTACCATT TATTCGAACT TCGGTCGATC ATGGTACTGC TCTGTCTTTA GCTGGGACAG GATTGGCAAA AAGCTCAAGT TTAAATGTCG CTGTCGATTT AGCTTTATCC TTGGCAGCGA GTTAA
Details for AcbaC.00116.a.B1.GE38587
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV IVLPLYVTSG EPAGIGPDIC LSLAKRVDER PVVVLADRNL LQQRAQKLGL DIKFLEYSGQ AESSLQGELY IEHVPLETIV VDGQLNAANA AYVLEQLRRS ADYAMSGKSV GVATAPVQKS VINDAGILFS GHTEYYQEFA GVERVVMMLA TKTLRVALAT THLPLRDVAD AITKERLHQV IDILLHDLKT KFKINDPRVL VCGLNPHAGE DGYLGREEID TINPVLESYR SQGVKLSLSL PADTLFTPEH LKNADAVLAM YHDQGLPVLK SQGFGEAINI TLGLPFIRTS VDHGTALSLA GTGLAKSSSL NVAVDXXXXL AAS
Validated NT Sequence
ttactcactt aactcgctgc caaggnnnnn nnnaaatcga cagcgacatt taaacttgag ctttttgcca atcctgtccc agctaaagac agagcagtac catgatcgac cgaagttcga ataaatggta agcccaatgt gatattgatt gcttcaccaa aaccctgtga ttttaacaca ggtaagcctt gatcatggta catcgcaaga acagcgtcag catttttaag atgttcaggc gtaaataaag tatcagctgg caaacttaaa cttaatttaa ctccctgtga ccggtagctc tcaagtaccg gattgatcgt atcaatctct tcacgtccca aataaccatc ttcgcctgca tgaggattta acccacatac taagactcga ggatcattaa ttttaaactt ggtttttaag tcgtgaagta aaatatcaat cacttgatgc aagcgttctt tggtaattgc atctgcaaca tctcttaatg gtaaatgagt ggtcgccaga gccacccgca acgttttcgt tgccagcatc ataacaacac gctctacgcc tgcaaattct tgataatatt cagtatgtcc actaaataaa atccctgcat cattaatcac cgacttttga acaggagcag tggctacacc gacgctctta ccagacatag cataatcagc tgagcgacgt aattgctcta atacataagc ggcattagca gcattaagct gtccatcaac gactatagtt tctaggggta catgttctat ataaagctcg ccttgcaaag acgattcagc ttgtccgcta tactctaaaa atttaatgtc tagaccaagc ttttgagcgc gttgttgtaa taaatttcga tcagccaaaa ccacaacagg acgttcatca acccgttttg ccagacttaa acaaatatct ggaccaatac cggcaggttc accggaagtg acatataaag gcagcacaat caccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHVI VLPLYVTSGE PAGIGPDICL SLAKRVDERP VVVLADRNLL QQRAQKLGLD IKFLEYSGQA ESSLQGELYI EHVPLETIVV DGQLNAANAA YVLEQLRRSA DYAMSGKSVG VATAPVQKSV INDAGILFSG HTEYYQEFAG VERVVMMLAT KTLRVALATT HLPLRDVADA ITKERLHQVI DILLHDLKTK FKINDPRVLV CGLNPHAGED GYLGREEIDT INPVLESYRS QGVKLSLSLP ADTLFTPEHL KNADAVLAMY HDQGLPVLKS QGFGEAINIT LGLPFIRTSV DHGTALSLAG TGLAKSSSLN VAVDLALSLA AS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgattg tgctgccttt atatgtcact tccggtgaac ctgccggtat tggtccagat atttgtttaa gtctggcaaa acgggttgat gaacgtcctg ttgtggtttt ggctgatcga aatttattac aacaacgcgc tcaaaagctt ggtctagaca ttaaattttt agagtatagc ggacaagctg aatcgtcttt gcaaggcgag ctttatatag aacatgtacc cctagaaact atagtcgttg atggacagct taatgctgct aatgccgctt atgtattaga gcaattacgt cgctcagctg attatgctat gtctggtaag agcgtcggtg tagccactgc tcctgttcaa aagtcggtga ttaatgatgc agggatttta tttagtggac atactgaata ttatcaagaa tttgcaggcg tagagcgtgt tgttatgatg ctggcaacga aaacgttgcg ggtggctctg gcgaccactc atttaccatt aagagatgtt gcagatgcaa ttaccaaaga acgcttgcat caagtgattg atattttact tcacgactta aaaaccaagt ttaaaattaa tgatcctcga gtcttagtat gtgggttaaa tcctcatgca ggcgaagatg gttatttggg acgtgaagag attgatacga tcaatccggt acttgagagc taccggtcac agggagttaa attaagttta agtttgccag ctgatacttt atttacgcct gaacatctta aaaatgctga cgctgttctt gcgatgtacc atgatcaagg cttacctgtg ttaaaatcac agggttttgg tgaagcaatc aatatcacat tgggcttacc atttattcga acttcggtcg atcatggtac tgctctgtct ttagctggga caggattggc aaaaagctca agtttaaatg tcgctgtcga tttagcttta tccttggcag cgagttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AcbaC.00116.a.B1.PS02359
PURIFICATION DATe: 3/17/2015
CONCENTRATION: 60.74mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV IVLPLYVTSG EPAGIGPDIC LSLAKRVDER PVVVLADRNL LQQRAQKLGL DIKFLEYSGQ AESSLQGELY IEHVPLETIV VDGQLNAANA AYVLEQLRRS ADYAMSGKSV GVATAPVQKS VINDAGILFS GHTEYYQEFA GVERVVMMLA TKTLRVALAT THLPLRDVAD AITKERLHQV IDILLHDLKT KFKINDPRVL VCGLNPHAGE DGYLGREEID TINPVLESYR SQGVKLSLSL PADTLFTPEH LKNADAVLAM YHDQGLPVLK SQGFGEAINI TLGLPFIRTS VDHGTALSLA GTGLAKSSSL NVAVDXXXXL AAS
Validated NT Sequence
ttactcactt aactcgctgc caaggnnnnn nnnaaatcga cagcgacatt taaacttgag ctttttgcca atcctgtccc agctaaagac agagcagtac catgatcgac cgaagttcga ataaatggta agcccaatgt gatattgatt gcttcaccaa aaccctgtga ttttaacaca ggtaagcctt gatcatggta catcgcaaga acagcgtcag catttttaag atgttcaggc gtaaataaag tatcagctgg caaacttaaa cttaatttaa ctccctgtga ccggtagctc tcaagtaccg gattgatcgt atcaatctct tcacgtccca aataaccatc ttcgcctgca tgaggattta acccacatac taagactcga ggatcattaa ttttaaactt ggtttttaag tcgtgaagta aaatatcaat cacttgatgc aagcgttctt tggtaattgc atctgcaaca tctcttaatg gtaaatgagt ggtcgccaga gccacccgca acgttttcgt tgccagcatc ataacaacac gctctacgcc tgcaaattct tgataatatt cagtatgtcc actaaataaa atccctgcat cattaatcac cgacttttga acaggagcag tggctacacc gacgctctta ccagacatag cataatcagc tgagcgacgt aattgctcta atacataagc ggcattagca gcattaagct gtccatcaac gactatagtt tctaggggta catgttctat ataaagctcg ccttgcaaag acgattcagc ttgtccgcta tactctaaaa atttaatgtc tagaccaagc ttttgagcgc gttgttgtaa taaatttcga tcagccaaaa ccacaacagg acgttcatca acccgttttg ccagacttaa acaaatatct ggaccaatac cggcaggttc accggaagtg acatataaag gcagcacaat caccatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHVI VLPLYVTSGE PAGIGPDICL SLAKRVDERP VVVLADRNLL QQRAQKLGLD IKFLEYSGQA ESSLQGELYI EHVPLETIVV DGQLNAANAA YVLEQLRRSA DYAMSGKSVG VATAPVQKSV INDAGILFSG HTEYYQEFAG VERVVMMLAT KTLRVALATT HLPLRDVADA ITKERLHQVI DILLHDLKTK FKINDPRVLV CGLNPHAGED GYLGREEIDT INPVLESYRS QGVKLSLSLP ADTLFTPEHL KNADAVLAMY HDQGLPVLKS QGFGEAINIT LGLPFIRTSV DHGTALSLAG TGLAKSSSLN VAVDLALSLA AS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGATTG TGCTGCCTTT ATATGTCACT TCCGGTGAAC CTGCCGGTAT TGGTCCAGAT ATTTGTTTAA GTCTGGCAAA ACGGGTTGAT GAACGTCCTG TTGTGGTTTT GGCTGATCGA AATTTATTAC AACAACGCGC TCAAAAGCTT GGTCTAGACA TTAAATTTTT AGAGTATAGC GGACAAGCTG AATCGTCTTT GCAAGGCGAG CTTTATATAG AACATGTACC CCTAGAAACT ATAGTCGTTG ATGGACAGCT TAATGCTGCT AATGCCGCTT ATGTATTAGA GCAATTACGT CGCTCAGCTG ATTATGCTAT GTCTGGTAAG AGCGTCGGTG TAGCCACTGC TCCTGTTCAA AAGTCGGTGA TTAATGATGC AGGGATTTTA TTTAGTGGAC ATACTGAATA TTATCAAGAA TTTGCAGGCG TAGAGCGTGT TGTTATGATG CTGGCAACGA AAACGTTGCG GGTGGCTCTG GCGACCACTC ATTTACCATT AAGAGATGTT GCAGATGCAA TTACCAAAGA ACGCTTGCAT CAAGTGATTG ATATTTTACT TCACGACTTA AAAACCAAGT TTAAAATTAA TGATCCTCGA GTCTTAGTAT GTGGGTTAAA TCCTCATGCA GGCGAAGATG GTTATTTGGG ACGTGAAGAG ATTGATACGA TCAATCCGGT ACTTGAGAGC TACCGGTCAC AGGGAGTTAA ATTAAGTTTA AGTTTGCCAG CTGATACTTT ATTTACGCCT GAACATCTTA AAAATGCTGA CGCTGTTCTT GCGATGTACC ATGATCAAGG CTTACCTGTG TTAAAATCAC AGGGTTTTGG TGAAGCAATC AATATCACAT TGGGCTTACC ATTTATTCGA ACTTCGGTCG ATCATGGTAC TGCTCTGTCT TTAGCTGGGA CAGGATTGGC AAAAAGCTCA AGTTTAAATG TCGCTGTCGA TTTAGCTTTA TCCTTGGCAG CGAGTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT